Li M.,Institute of Agricultural Science in the Regions along Yangtze River of Jiangsu |
Yu C.,Nantong University |
Wang Y.,Nantong University |
Li W.,Nantong University |
And 7 more authors.
Current Genomics | Year: 2014
Willows are one of the most important tree species for landscaping, biofuel and raw timber. Screening salttolerant willow varieties is an effective approach to balance wood supply and demand. However, more salt-tolerant willow varieties are required and little is known regarding the mechanism of salt tolerance at the gene expression level. In this paper, two willow varieties were studies in terms of their differences in salt-tolerances and mechanism of salt tolerance at the level of VP1 gene expression. The results showed that Salix L0911 (L0911) had higher biomass than Salix matsudana (SM), and salt injuries were less severe in L0911 than in SM. The activities of peroxidase and superoxide dismutase, as well as the contents of soluble protein and proline, were higher in L0911 than in SM, whereas the contents of Na+ and K+, as well as the Na+/K+ ratio, were lower in L0911 than in SM. Two VP1 genes (VP1.1 and VP1.2) cloned in L0911 and SM had similar sequences and structures. VP1.1 and VP1.2 belonged to different subgroups. Total expression levels of the VP1.1 gene in both roots and leaves of L0911 were higher than that in SM under normal conditions. Under salt stress, expression of VP1 in SM roots initially increased and then decreased, whereas the expression of VP1 in leaves of L0911 and SM, as well as in roots of L0911, decreased with increasing salt concentrations. This study increased our understanding of the salt-tolerance mechanism of willow and may facilitate the selection of salt-tolerant willow resources. © 2014 Bentham Science Publishers.
Wang Y.,Institute of Agricultural Science in the Regions Along Yangtze River of Jiangsu |
Yuan H.,Beijing Forestry University |
Li M.,Institute of Agricultural Science in the Regions Along Yangtze River of Jiangsu |
Li Y.,Institute of Agricultural Science in the Regions Along Yangtze River of Jiangsu |
And 4 more authors.
Israel Journal of Plant Sciences | Year: 2013
Willows are one of the most important landscaping, biofuel, and raw timber tree species in the world. Cultivating fast-growing, salt-tolerant willow varieties is an effective way to balance wood supply and demand in China. This paper presents a comparative evaluation of the phenotypic development and physiological responses of two willow varieties, Salix L0911 (L0911) and Salix matsudana (SM), to salt stress. L0911 developed more biomass than SM and demonstrated higher tolerance to long-term exposures to NaCl-salinity. The two varieties demonstrated similar trends of physiological changes under salt stress, but the extent of the induced responses were higher in L0911, suggesting the involvement of specific responses in the salt-tolerant mechanism. Specifically, the activities of superoxide dismutase, peroxidase, soluble proteins and proline contents were higher in L0911 compared to SM, and the content of malondialdehyde and Na+ and Na+/K+ ratios were lower, demonstrating that the improved tolerance to salinity in L0911 is based on antioxidant response and improved ion selectivity. © 2014 Taylor & Francis.