Institute of Agricultural Genetics

Hanoi, Vietnam

Institute of Agricultural Genetics

Hanoi, Vietnam
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Tanaka M.,Kagawa University | Van P.T.,Ehime University | Teixeira da Silva J.A.,Kagawa University | Ham L.H.,Institute of Agricultural Genetics
Biotechnology and Biotechnological Equipment | Year: 2010

The purpose of this study was to create a new magnetic field (MF) system which is simple, safe, easy to set up and saves space in a tissue culture room or greenhouse. We tested this MF system to study the effects of three different MF intensities (0.1 Tesla (T), 0.15 T and 0.2 T) combined with two polarities (North (N) and South (S) pole) on the proliferation of Phalaenopsis Gallant Beau 'George Vazquez' protocorm-like bodies (PLBs). Control explants that were not exposed to MFs tended to become necrotic, while The 0.2 T-N pole MF combination significantly increased the fresh and dry weights of PLB clusters, more than the observed for the controls and other treatments.

News Article | November 15, 2016

Asia’s only starch summit features topics concerning price impact on Asian Starch Markets by Simon Bentley, Managing Director of Commoditia who also examines the competitive starch raw materials - corn and cassava. He shares vital insights on the pockets of growth in Asia and the impact of feedstock price fall on processors looking to expand in the region. Lief Chang, Senior Analyst at Rabobank reflects on China’s Corn Reform Policy and what It means for its corn starch processing Industry. The highly competitive cassava and corn starch feedstock markets are examined in sessions on: As the world’s second largest cassava exporter, Vietnam is the apt venue and major focus for the summit with analysis of its New Policy Agenda for Developing the Cassava and Value Chain Industry presented by Ministry of Agriculture & Rural Development. Vedan – one of the leading players in Vietnam shares its perspective on ‘Tapioca Starch and Downstream Industries’ in the country, spotlighting on its modified starch and starch syrup development. ‘Biotechnology for Sustainable Cassava Production’ by Vietnam’s Institute of Agricultural Genetics and ‘Sustainable Production of Cassava and Challenges on Getting from Farm to Factory’ by CIAT are two important discussion topics at the January summit. Other value added topics are: Organized by Centre for Management Technology (CMT), an exhibition featuring Paglierani, VetterTec, G - FORCE ASIA and Trislot will be held alongside the summit. For more information about how to participate as delegate, sponsor or exhibitor, visit 6th Starch World Asia website or contact Ms. Huiyan at +65 6346 9113.

Nguyen T.H.N.,Leibniz University of Hanover | Nguyen T.H.N.,Institute of Agricultural Genetics | Schulz D.,Leibniz University of Hanover | Winkelmann T.,Leibniz University of Hanover | Debener T.,Leibniz University of Hanover
Plant Cell Reports | Year: 2017

Key Message: We analysed the capacity to regenerate adventitious shoots in 96 rose genotypes and found 88 SNP markers associated with QTLs, some of which are derived from candidate genes for shoot regeneration.Abstract: In an association panel of 96 rose genotypes previously analysed for petal colour, we conducted a genome-wide association study on the capacity of leaf petioles for direct shoot regeneration. Shoot regeneration rate and shoot ratio (number of shoots/total number of explants) were used as phenotypic descriptors for regeneration capacity. Two independent experiments were carried out with six replicates of ten explants each. We found significant variation between the genotypes ranging from 0.88 to 88.33% for the regeneration rate and from 0.008 to 1.2 for the shoot ratio, which exceeded the rates reported so far. Furthermore, we found 88 SNP markers associated with either the shoot regeneration rate or the shoot ratio. In this association analysis, we found 12 SNP markers from ESTs (expressed sequence tags) matching known candidate genes that are involved in shoot morphogenesis. The best markers explained more than 51% of the variance in the shoot regeneration rate and more than 0.65 of the variance in the shoot regeneration ratio between the homozygote marker classes. The genes underlying some of the best markers such as a GT-transcription factor or an LRR receptor-like protein kinase are novel candidate genes putatively involved in the observed phenotypic differences. The associated markers were mapped to the closely related genome of Fragaria vesca and revealed many distinct clusters, which also comprised the known candidate genes that functioned in the organogenesis of plant shoots. However, the validation of candidate genes and their functional relationship to shoot regeneration require further analysis in independent rose populations and functional analyses. © 2017 Springer-Verlag GmbH Germany

Tuteja N.,International Center for Genetic Engineering and Biotechnology | Banu Mst.S.A.,International Center for Genetic Engineering and Biotechnology | Huda K.Md.K.,International Center for Genetic Engineering and Biotechnology | Gill S.S.,MD University | And 4 more authors.
PLoS ONE | Year: 2014

Background: The DEAD-box helicases are required mostly in all aspects of RNA and DNA metabolism and they play a significant role in various abiotic stresses, including salinity. The p68 is an important member of the DEAD-box proteins family and, in animal system, it is involved in RNA metabolism including pre-RNA processing and splicing. In plant system, it has not been well characterized. Here we report the cloning and characterization of p68 from pea (Pisum sativum) and its novel function in salinity stress tolerance in plant. Results: The pea p68 protein self-interacts and is localized in the cytosol as well as the surrounding of cell nucleus. The transcript of pea p68 is upregulated in response to high salinity stress in pea. Overexpression of p68 driven by constitutive cauliflower mosaic virus-35S promoter in tobacco transgenic plants confers enhanced tolerances to salinity stress by improving the growth, photosynthesis and antioxidant machinery. Under stress treatment, pea p68 overexpressing tobacco accumulated higher K+ and lower Na+ level than the wild-type plants. Reactive oxygen species (ROS) accumulation was remarkably regulated by the overexpression of pea p68 under salinity stress conditions, as shown from TBARS content, electrolyte leakage, hydrogen peroxide accumulation and 8-OHdG content and antioxidant enzyme activities. Conclusions: To the best of our knowledge this is the first direct report, which provides the novel function of pea p68 helicase in salinity stress tolerance. The results suggest that p68 can also be exploited for engineering abiotic stress tolerance in crop plants of economic importance. © 2014 Tuteja et al.

Coudert Y.,Montpellier University | Perin C.,CIRAD - Agricultural Research for Development | Courtois B.,CIRAD - Agricultural Research for Development | Khong N.G.,Montpellier University | And 2 more authors.
Trends in Plant Science | Year: 2010

Cereals possess a fibrous root system that is mainly composed of crown roots that emerge postembryonically from the nodes of the stem. Because the root system is not directly accessible and consequently difficult to study, it remains a target for breeders to improve the ability of plants to exploit the mineral and water resources of the soil. Breeding for root architecture necessitates identifying the genetic determinants of root development. This research is now underway in cereals, particularly in rice, the monocot model species. In this review, we examine recent data identifying genes that govern root development in cereals, such as ARL1/CRL1 in rice and RTCS in maize which encodes a conserved lateral organ boundary domain transcription factor involved in crown root initiation and development in response to auxin. Finally, we discuss the detection and validation of root development quantitative trait loci. © 2010 Elsevier Ltd. All rights reserved.

Meldau D.G.,Max Planck Institute for Chemical Ecology | Meldau S.,Max Planck Institute for Chemical Ecology | Meldau S.,German Center for Integrative Biodiversity Research | Hoang L.H.,Institute of Agricultural Genetics | And 3 more authors.
Plant Cell | Year: 2013

Bacillus sp B55, a bacterium naturally associated with Nicotiana attenuata roots, promotes growth and survival of wild-type and, particularly, ethylene (ET)-insensitive 35S-ethylene response1 (etr1) N. attenuata plants, which heterologously express the mutant Arabidopsis thaliana receptor ETR1-1. We found that the volatile organic compound (VOC) blend emitted by B55 promotes seedling growth, which is dominated by the S-containing compound dimethyl disulfide (DMDS). DMDS was depleted from the headspace during cocultivation with seedlings in bipartite Petri dishes, and 35S was assimilated from the bacterial VOC bouquet and incorporated into plant proteins. In wild-type and 35S-etr1 seedlings grown under different sulfate (SO4 -2) supply conditions, exposure to synthetic DMDS led to genotype-dependent plant growth promotion effects. For the wild type, only S-starved seedlings benefited from DMDS exposure. By contrast, growth of 35S-etr1 seedlings, which we demonstrate to have an unregulated S metabolism, increased at all SO4 -2 supply rates. Exposure to B55 VOCs and DMDS rescued many of the growth phenotypes exhibited by ET-insensitive plants, including the lack of root hairs, poor lateral root growth, and low chlorophyll content. DMDS supplementation significantly reduced the expression of S assimilation genes, as well as Met biosynthesis and recycling. We conclude that DMDS by B55 production is a plant growth promotion mechanism that likely enhances the availability of reduced S, which is particularly beneficial for wild-type plants growing in S-deficient soils and for 35S-etr1 plants due to their impaired S uptake/assimilation/metabolism. © 2013 American Society of Plant Biologists. All rights reserved.

Meldau D.G.,Max Planck Institute for Chemical Ecology | Long H.H.,Max Planck Institute for Chemical Ecology | Long H.H.,Institute of Agricultural Genetics | Baldwin L.T.,Max Planck Institute for Chemical Ecology
Frontiers in Plant Science | Year: 2012

Many plants have intimate relationships with soil microbes, which improve the plant's growth and fitness through a variety of mechanisms. Bacillus sp. isolates are natural root-associated bacteria, isolated from Nicotiana attenuata plant roots growing in native soils. A particular isolate B55, was found to have dramatic plant growth promotion (PGP) effects on wild type (WT) and transgenic plants impaired in ethylene (ET) perception (35S-etr1), the genotype from which this bacterium was first isolated. B55 not only improves N. attenuata growth under in vitro, glasshouse, and field conditions, but it also "rescues" many of the deleterious phenotypes associated with ET insensitivity. Most notably, B55 dramatically increases the growth and survival of 35S-etr1 plants under field conditions. To our knowledge, this is the first demonstration of a PGP effect in a native plant-microbe association under natural conditions. Our study demonstrates that this facultative mutualistic plant-microbe interaction should be viewed as part of the plant's extended phenotype. Possible modalities of recruitment and mechanisms of PGP are discussed. © 2012 Meldau, Longand Baldwin.

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