Institute Oceanografia IO

FURG, Brazil

Institute Oceanografia IO

FURG, Brazil

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Santos R.A.,Grande Rio University | Tesser M.B.,Institute Oceanografia IO | Monserrat J.M.,Grande Rio University
Fish Physiology and Biochemistry | Year: 2016

Lipoic acid (LA) is an antioxidant that also favors glucose uptake in mammals. Until now, there are no studies evaluating the potential effect of this molecule on glycemic control in fish. It was evaluated LA effects on glucose uptake in common carp Cyprinus carpio fed with carbohydrate diets from two carbohydrate sources: glucose (GLU) and starch (STA), and supplemented or not with LA, being the diets: +GLU/−LA (GLU); +GLU/+LA (GLU + LA); +STA/−LA (STA); and +STA/+LA (STA + LA). Carp juveniles (6.5 ± 0.1 g) were fed with each diet ad libitum 4 times a day, during 68 days. Muscle glycogen concentration was higher (p < 0.05) in GLU and GLU + LA than in STA and STA + LA groups. On fish fed with starch, muscle cholesterol and triglyceride concentrations were higher (p < 0.05) in fish fed diets supplemented with LA. Muscle protein levels were higher in fish fed with LA, independent of the diet carbohydrate source. Lipid peroxidation was significantly reduced (p < 0.05) in fish muscle on fish fed the STA + LA diets when compared with the STA diet. Our findings indicate that LA modulates lipid, proteins and carbohydrate metabolism together with the well-known antioxidant effect. Also, LA showed to enhance starch utilization taking into account muscle cholesterol and triglyceride levels. © 2016 Springer Science+Business Media Dordrecht


da Silva Martins A.C.,Grande Rio University | Artigas Flores J.,Grande Rio University | Porto C.,Grande Rio University | Wasielesky Junior W.,Grande Rio University | And 2 more authors.
Marine and Freshwater Behaviour and Physiology | Year: 2015

Shrimp (Litopenaeus vannamei) reared in a conventional seawater (SW) aquarium system SW were compared with those raised in a biofloc technology (BFT) system. After 30 days, the L. vannamei shrimp were euthanized and samples of gills, hepatopancreas and muscle were dissected. Statistical analysis was performed using bi-factorial ANOVA, with the factors of the treatment (shrimp reared in SW or in a BFT system) and organs (gills, hepatopancreas and muscles). No differences (p > 0.05) in glutathione-S-transferase activity were observed between shrimp reared in SW and shrimp reared in BFT (p < 0.05). Glutathione levels were lower (p < 0.05) in the gills and hepatopancreas of shrimp reared in the BFT system, suggesting changes in the antioxidant composition of these organs. Lipid peroxidation levels were higher in the hepatopancreas than in muscle in shrimp reared in SW (p < 0.05) and this difference was not observed in organisms reared in the BFT system (p > 0.05). Overall, the results showed that rearing shrimp in the BFT system altered both their antioxidant and oxidative damage responses. This indicates that some microbial communities in BFT systems can influence the redox state of L. vannamei. © 2015 Taylor & Francis.


Kutter M.T.,Grande Rio University | Romano L.A.,Grande Rio University | Romano L.A.,Institute Oceanografia IO | Ventura-Lima J.,Grande Rio University | And 5 more authors.
Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology | Year: 2014

Lipoic acid (LA) is a disulfide-containing compound derived from octanoic acid that is synthesized in mitochondria. This molecule acts as a co-factor for mitochondrial enzymes that catalyze oxidative decarboxylation reactions. Several antioxidant properties of LA enable it to be considered as an "ideal antioxidant", having diverse benefits that allow it to deal with environmental or biological stress. Some of the effects induced by LA in aquatic organisms render it suitable for use in aquaculture. However, it is necessary to determine the appropriate dose(s) to be used with different species and even organs to maximize the beneficial antioxidant and detoxifying effects and to minimize the pro-oxidant toxic effects. This review analyzes and compiles existing data from aquatic organisms in which both benefits and drawbacks of LA have been described. © 2014 Elsevier Inc.


Kutter M.T.,Grande Rio University | Monserrat J.M.,Grande Rio University | Monserrat J.M.,Institute Ciencias Biologicas ICB | Tesser M.B.,Grande Rio University | Tesser M.B.,Institute Oceanografia IO
Aquaculture | Year: 2012

This study evaluated the effect of four doses of α-lipoic acid (LA) from dietary supplementation on growth performance, body composition and anti-oxidant status in the fish Trachinotus marginatus. The fish fed for 42days with a diet containing different concentrations of LA: 0mgLAkg -1 dry food, 316.4mgLAkg -1 dry food, 524mgLAkg -1 dry food, 890.4mgLAkg -1 dry food and 1367.3mgLAkg -1 dry food. High doses (890.4 and 1367.3mgLAkg -1) of dietary LA significantly reduced the growth (p<0.05), the specific growth rate and the protein efficiency ratio. When administered in high doses of LA, supplementation significantly decreased the feed intake (p<0.05), although the feed conversion ratio increased (p<0.05) in these groups. The carcass composition exhibited the highest lipid values in the control group and in the fish exposed to 316.4 and 524mgLAkg -1 dry food (p<0.05). The LA dietary supplementation increased the brain activity of glutathione-S-transferase (GST) when administered at a dose of 890.4mgLAkg -1 dry food, and the lipid peroxidation in muscle was reduced (at all LA doses) (p<0.05). The results obtained regarding growth, antioxidant status and lipid and protein metabolism indicated that LA could be supplemented in diets for T. marginatus at doses between 316.4 and 524mgLAkg -1 dry food. © 2012 Elsevier B.V.


Gonzalez-Durruthy M.,Grande Rio University | Werhli A.V.,Grande Rio University | Cornetet L.,Grande Rio University | Machado K.S.,Grande Rio University | And 6 more authors.
RSC Advances | Year: 2016

Interactions between the single walled carbon nanotube (SWCNT) family and a mitochondrial ADP/ATP carrier (ANT-1) were evaluated using constitutional (functional groups, number of carbon atoms, etc.) and electronic nanodescriptors defined by (n, m)-Hamada indexes (armchair, zig-zag and chiral). The Free Energy of Binding (FEB) was determined by molecular docking simulation and the results showed that FEB was statistically more negative (p < 0.05), following the order SWCNT-COOH > SWCNT-OH > SWCNT, suggesting that polar groups favor the anchorage to ANT-1. In this regard, it was showed that key ANT-1 amino acids (Arg 79, Asn 87, Lys 91, Arg 187, Arg 234 and Arg 279) responsible for ADP-transport were conserved in ANT-1 from different species examined to predict SWCNT interactions, including shrimp Litopenaeus vannamei and fish Danio rerio commonly employed in ecotoxicology. The SWCNT-ANT-1 inter-atomic distances for the key ANT-1 amino acids were similar to that with carboxyatractyloside, a classical inhibitor of ANT-1. Significant linear relationships between FEB and n-Hamada index were found for zig-zag SWCNT and SWCNT-COOH (R2 = 0.95 in both cases). A Perturbation Theory-Nano-Quantitative Structure-Binding Relationship (PT-NQSBR) model was fitted that was able to distinguish between strong (FEB < -14.7 kcal mol-1) and weak (FEB ≥ -14.7 kcal mol-1) SWCNT-ANT-1 interactions. A simple ANT-1-inhibition respiratory assay employing mitochondria suspension from L. vannamei, showed good accordance with the predicted model. These results indicate that this methodology can be employed in massive virtual screenings and used for making regulatory decisions in nanotoxicology. © The Royal Society of Chemistry 2016.


Martins A.C.B.,Grande Rio University | Kinas P.G.,Grande Rio University | Kinas P.G.,Institute Matematica | Marangoni J.C.,Grande Rio University | And 4 more authors.
Aquatic Ecology | Year: 2015

The present study characterizes the fish assemblage in the surf zone of Cassino Beach, Rio Grande, Brazil, and analyzes temporal fluctuations in richness and abundance of these species in medium (months) and long terms (years), associating them also with abiotic covariates. Data were collected monthly between 1996 and 2012 at two locations. Bayesian generalized additive models (GAMs) were used as statistical tool, placing this study among few that have used Bayesian GAMs in Ecology. Our results show a decrease in both species richness and abundance of the most representative species, over the last 16 years, but no significant distinction between locations. Water temperature and salinity along with seasonality were the statistically most influential explanatory covariates to describe fluctuations in richness and abundance. Higher discharge rates of the three main rivers that flow into Patos Lagoon (Jacuí, Taquari, Camaquã) were associated with increased richness and abundance of some species in the assemblage. Hence, our findings show that medium- and long-term fluctuations in richness and species abundance are controlled by abiotic factors related to seasonal cycles (temperature) and productivity of the ecosystem. Long-term changes seem to be also related to man-induced factors and climate change; but further research is needed. © 2015, Springer Science+Business Media Dordrecht.


Ferreira J.L.R.,Grande Rio University | Ferreira J.L.R.,Institute Ciencias Biologicas ICB | Ferreira J.L.R.,Brazilian National Council for Scientific and Technological Development | Lonne M.N.,University of Buenos Aires | And 10 more authors.
Aquatic Toxicology | Year: 2014

Compounds from the nanotechnology industry, such as carbon-based nanomaterials, are strong candidates to contaminate aquatic environments because their production and disposal have exponentially grown in a few years. Previous evidence shows that fullerene C60, a carbon nanomaterial, can facilitate the intake of metals or PAHs both in vivo and in vitro, potentially amplifying the deleterious effects of these toxicants in organisms. The present work aimed to investigate the effects of fullerene C60 in a Danio rerio (zebrafish) hepatocyte cell lineage exposed to benzo[a]pyrene (BaP) in terms of cell viability, oxidative stress parameters and BaP intracellular accumulation. Additionally, a computational docking was performed to investigate the interaction of the fullerene C60 molecule with the detoxificatory and antioxidant enzyme πGST. Fullerene C60 provoked a significant (p<0.05) loss in cellular viability when co-exposed with BaP at 0.01, 0.1 and 1.0μg/L, and induced an increase (p<0.05) in BaP accumulation in the cells after 3 and 4h of exposure. The levels of reactive oxygen species (ROS) in the cells exposed to BaP were diminished (p<0.05) by the fullerene addition, and the increase of the GST activity observed in the BaP-only treated cells was reduced to the basal levels by co-exposure to fullerene. However, despite the potential of the fullerene molecule to inhibit π GST activity, demonstrated by the computational docking, the nanomaterial did not significantly (p>0.05) alter the enzyme activity when added to GST purified extracts from the zebrafish hepatocyte cells. These results show that fullerene C60 can increase the intake of BaP into the cells, decreasing cell viability and impairing the detoxificatory response by phase II enzymes, such as GST, and this latter effect should be occurring at the transcriptional level. © 2013 Elsevier B.V.


Enamorado A.D.,Grande Rio University | Martins A.C.,Grande Rio University | Flores J.A.,Grande Rio University | Tesser M.B.,Grande Rio University | And 5 more authors.
Comparative Biochemistry and Physiology -Part A : Molecular and Integrative Physiology | Year: 2015

The current study aimed to evaluate the influence of lipoic acid (LA) supplementation (439.84 ± 6.71. mg LA/kg feed) on antioxidants responses throughout the time in intestine, liver and muscle of juvenile common carp Cyprinus carpio. Two experimental groups were fed during four weeks with a diet with or without LA. Glutathione-S-transferase (GST) activity, glutathione (GSH) content, antioxidant capacity against peroxyl radicals (ACAP) and lipid peroxidation (TBARS) were evaluated in these organs. Also, a technique to measure protein disulfide bonds and sulfhydryl groups was optimized for intestine samples. GST activity was significantly higher (p. <0.05) in intestine after two weeks of supplementation. GSH content was also significantly higher (p. <0.05) in intestine, liver and muscle of fish fed with LA after two and three weeks, respectively. Total capacity antioxidant against peroxyl radicals was significantly increased (p. <0.05) in the muscle of animals fed with LA after the fourth week. Concentration of disulfide bonds was higher in the intestine of fish fed with LA but this group also showed higher concentration of sulfhydryl groups (p. <0.05). It is concluded that supplementation with LA is a safe strategy to induce antioxidant responses and improves the antioxidant status in different organs of common carp. Two week of supplementation are required to induce antioxidant responses in intestine and liver and three week for muscle. © 2015 Elsevier Inc.


PubMed | Grande Rio University and Institute Oceanografia IO
Type: Journal Article | Journal: Fish physiology and biochemistry | Year: 2016

Lipoic acid (LA) is an antioxidant that also favors glucose uptake in mammals. Until now, there are no studies evaluating the potential effect of this molecule on glycemic control in fish. It was evaluated LA effects on glucose uptake in common carp Cyprinus carpio fed with carbohydrate diets from two carbohydrate sources: glucose (GLU) and starch (STA), and supplemented or not with LA, being the diets: +GLU/-LA (GLU); +GLU/+LA (GLU+LA); +STA/-LA (STA); and +STA/+LA (STA+LA). Carp juveniles (6.50.1g) were fed with each diet ad libitum 4 times a day, during 68days. Muscle glycogen concentration was higher (p<0.05) in GLU and GLU+LA than in STA and STA+LA groups. On fish fed with starch, muscle cholesterol and triglyceride concentrations were higher (p<0.05) in fish fed diets supplemented with LA. Muscle protein levels were higher in fish fed with LA, independent of the diet carbohydrate source. Lipid peroxidation was significantly reduced (p<0.05) in fish muscle on fish fed the STA+LA diets when compared with the STA diet. Our findings indicate that LA modulates lipid, proteins and carbohydrate metabolism together with the well-known antioxidant effect. Also, LA showed to enhance starch utilization taking into account muscle cholesterol and triglyceride levels.


PubMed | National University of Luján, Grande Rio University, University of Buenos Aires, Federal University of Santa Maria and Institute Oceanografia IO
Type: | Journal: Aquatic toxicology (Amsterdam, Netherlands) | Year: 2014

Compounds from the nanotechnology industry, such as carbon-based nanomaterials, are strong candidates to contaminate aquatic environments because their production and disposal have exponentially grown in a few years. Previous evidence shows that fullerene C60, a carbon nanomaterial, can facilitate the intake of metals or PAHs both in vivo and in vitro, potentially amplifying the deleterious effects of these toxicants in organisms. The present work aimed to investigate the effects of fullerene C60 in a Danio rerio (zebrafish) hepatocyte cell lineage exposed to benzo[a]pyrene (BaP) in terms of cell viability, oxidative stress parameters and BaP intracellular accumulation. Additionally, a computational docking was performed to investigate the interaction of the fullerene C60 molecule with the detoxificatory and antioxidant enzyme GST. Fullerene C60 provoked a significant (p<0.05) loss in cellular viability when co-exposed with BaP at 0.01, 0.1 and 1.0 g/L, and induced an increase (p<0.05) in BaP accumulation in the cells after 3 and 4h of exposure. The levels of reactive oxygen species (ROS) in the cells exposed to BaP were diminished (p<0.05) by the fullerene addition, and the increase of the GST activity observed in the BaP-only treated cells was reduced to the basal levels by co-exposure to fullerene. However, despite the potential of the fullerene molecule to inhibit GST activity, demonstrated by the computational docking, the nanomaterial did not significantly (p>0.05) alter the enzyme activity when added to GST purified extracts from the zebrafish hepatocyte cells. These results show that fullerene C60 can increase the intake of BaP into the cells, decreasing cell viability and impairing the detoxificatory response by phase II enzymes, such as GST, and this latter effect should be occurring at the transcriptional level.

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