Institute National Of Recherches En Genie Rural Eaux Et Forets

Ariana, Tunisia

Institute National Of Recherches En Genie Rural Eaux Et Forets

Ariana, Tunisia
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Ismail A.,Bizerte | Ismail A.,Institute National Of Recherches En Genie Rural Eaux Et Forets | Ismail A.,Center de Biotechnologie de la Technopole de Borj-Cedria | Ismail A.,Institute Superieur Deducation Et Of Formation Continue | And 12 more authors.
Current Bioactive Compounds | Year: 2012

The essential oil composition of Juniperus phoniceae, Pistacia vera and Pistacia terebinthus was analyzed by GC and GC-MS analysis and their bioherbicidal activity was evaluated against four weeds species, Sinapis arvensis, Trifolium campestre (dicots), Lolium rigidium and Phalaris canariensis (monocots). Qualitative and quantitative differences between oils were observed. All oils were rich in monoterpene hydrocarbons, the major constituents of J. phoniceae were α -pinene (49%) and α -terpinene (8%) and the major components in P. vera and P. terebinthus were α-pinene (16-19%), α-terpinene (32-41%) and limonene (4-25%). Tested oils strongly inhibited the germination and seedling growth of all weeds, in a dose dependent manner with dicots weeds being significantly more sensitive than monocots, indeed, at the dose of 4μl/ml, germination of S. arvensis and T. campestre was totally inhibited, while for the same dose, germination and seedling growth of L. rigidium and P. canariensis were partially reduced. Essential oils increased the level of proline and caused a severe electrolyte leakage from the roots of all tested weeds indicating membrane disruption and loss of integrity. Our results show that essential oils of forestry trees have an important phytotoxic effect on seed germination and seedling growth of weeds. The phytotoxic activity of the essential oils may be attributed to their relatively high content of monoterpene hydrocarbons. It can be suggested that these essential oils have the potential to be used as a bioherbicide. © 2012 Bentham Science Publishers


Amri I.,University of Carthage | Amri I.,Institute National Of Recherches En Genie Rural Eaux Et Forets | Lamia H.,Institute National Of Recherches En Genie Rural Eaux Et Forets | Gargouri S.,French National Institute for Agricultural Research | And 5 more authors.
Natural Product Communications | Year: 2011

Essential oils isolated from needles of Pinus patula by hydrodistillation were analyzed by gas chromatography-flame ionization detection (GC-FID) and gas chromatography mass spectrometry (GC-MS). Thirty-eight compounds were identified, representing 98.3% of the total oil. The oil was rich in monoterpene hydrocarbons (62.4%), particularly α-pinene (35.2%) and β-phellandrene (19.5%). The in vitro antifungal assay showed that P. patula oil significantly inhibited the growth of 9 plant pathogenic fungi. The oil, when tested on Sinapis arvensis, Lolium rigidum, Phalaris canariensis and Trifolium campestre, completely inhibited seed germination and seedling growth of all species. Our preliminary results showed that P. patula essential oil could be valorized for the control of weeds and fungal plant diseases.


Kaderi M.,Institute National Agronomique Of Tunisie Inat | Kaderi M.,Institute National Of Recherches En Genie Rural Eaux Et Forets | Ben Hamouda G.,Institute National Of Recherches En Genie Rural Eaux Et Forets | Zaeir H.,Institute National Of Recherches En Genie Rural Eaux Et Forets | And 2 more authors.
Phytotherapie | Year: 2015

Ceratonia siliqua L. is a tree belonging to the family of Fabaceae. From the Middle East, the carob is an important ecologically industrial and ornamental tree. This fabaceae is known for its medicinal and therapeutic virtues such as the pharmacological activities of the phytochemical compounds founded in the biological extracts of the plant. © 2014, Springer-Verlag France.


Ayadi R.,Institute National Of Recherches En Genie Rural Eaux Et Forets | Hamrouni L.,Institute National Of Recherches En Genie Rural Eaux Et Forets | Hanana M.,Center de Biotechnologie de la Technopole de Borj-Cedria | Bouzid S.,Faculte des science de Tunis | And 2 more authors.
Industrial Crops and Products | Year: 2011

The present study report a protocol for the efficient in vitro propagation of kenaf (Hibiscus cannabinus L., an industrial crop having high cellulosic fiber content) on hormone free MS medium using the shoot apex and nodal explants. Shoot tips and nodes were isolated from 15 days old seedlings cultivated on MS medium. Different combinations and concentrations of auxin/cytokinin were used and added to the MS medium to assess the shoot and root induction of theses explants. Several subcultures were drived in order to enhance the multiplication rate. Healthy and well developed in vitro propagated shoots were transferred for acclimatization under greenhouse conditions in pots filled with different substrates (sand. +. compost or perlite). Our results showed that shoots could elongate and root within 4-6 weeks on MS basal medium without any callus formation. However, addition of growth regulators to the MS medium leaded to a decrease in shoot and root induction rates. Indeed, the highest shoot regeneration frequency (90.5%) was obtained on MS control medium. Elongated shoots were transferred onto the same hormone free MS medium using five subcultures where the multiplication rate reached the highest value (3.66) at the fifth and last step. The in vitro rooted plantlets were acclimatized in greenhouse and successfully transplanted to natural conditions with 70% survival. © 2010 Elsevier B.V.


Youzbachi N.,University of Tunis | Elfalleh W.,Laboratoire dAridoculture et des Cultures Oasiennes | Tlili N.,University of Tunis | Gregoire S.,French National Institute for Agricultural Research | And 7 more authors.
Journal of the Science of Food and Agriculture | Year: 2012

Background: In order to investigate new sources of dietary phytochemicals, recent studies have focused on underexploited seeds. In this study the total lipid contents, fatty acid profiles and levels of soluble proteins, minerals and antioxidants in seeds from 12 Acacia cyanophylla ecotypes commonly grown in Tunisia were determined. Results: Total lipids averaged 101.7 g kg -1 on a dry weight basis. Linoleic (61.11-65.45% of total fatty acid content), oleic (19.67-22.85%) and palmitic (9.18-9.98%) acids were the principal fatty acids. Smaller proportions of stearic (1.49-1.82%), vaccenic (1.13-2.05%) and palmitoleic (0.34-0.58%) acids were also quantified. Proteins (by Kjeldahl assay) averaged 107.2 g kg -1 on a dry weight basis. Total phenolics averaged 1.91 g gallic acid equivalent kg -1 dry weight (DW) and total flavonoids averaged 0.40 g rutin equivalent kg -1 DW. The free radical-scavenging activity determined by 2,2-diphenyl-1-picrylhydrazyl assay averaged 0.59 mmol L -1 Trolox equivalent antioxidant capacity (TEAC), while that determined by 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) assay averaged 0.28 mmol L -1 TEAC. Conclusion: The findings of this study confirm the presence of ω6 fatty acids at high levels in A. cyanophylla seeds. These metabolites could be used as such and/or extracted for the formulation of supplements and/or ingredients to provide a ratio close to the ideal for the ω3/ω6 balance. © 2012 Society of Chemical Industry.


Nasri N.,University of Tunis | Elfalleh W.,Institute des Regions Arides | Tlili N.,University of Tunis | Martine L.,University of Burgundy | And 4 more authors.
JAOCS, Journal of the American Oil Chemists' Society | Year: 2013

Seeds from 12 Acacia cyanophylla ecotypes, harvested in Tunisia, were examined for their seed oil contents of carotenoids, tocopherols and phytosterols. The average carotenoid content (lutein and zeaxanthin) was ca. 102 mg kg-1 of total extracted lipids. Lutein (ca. 97 mg kg-1 of total extracted lipids) was usually more abundant than zeaxanthin (ca. 5 mg kg-1 of total extracted lipids). The mean total tocopherol content was ca. 704 mg kg-1 of total extracted lipids. The main isomer was α-tocopherol, with more than 75 % of total tocopherols (ca. 528 mg kg -1 of total extracted lipids), followed by γ-tocopherol (ca. 168 mg kg-1 of total extracted lipids) and δ-tocopherol (ca. 86 mg kg-1 of total lipids). High levels of phytosterols (ca. 7.8 g kg-1 of total extracted lipids) were detected, among which β-sitosterol was the most abundant (47 %). All these results highlight the richness of carotenoids, tocopherols and sterols in A. cyanophylla seed oil, and imply that this species might constitute a potential resource for the development of functional foods. © 2012 AOCS.


Hannachi H.,Laboratoire dAridoculture et cultures oasiennes | Elfalleh W.,Laboratoire dAridoculture et cultures oasiennes | Ennajeh I.,Laboratoire dAridoculture et cultures oasiennes | Laajel M.,Laboratoire dAridoculture et cultures oasiennes | And 4 more authors.
Journal of Medicinal Plant Research | Year: 2011

This study reported investigations on phytochemical screening and antioxidants activities of seeds from seven Acacia species. Storage proteins and mineral contents were determined. The seed extracts of Acacia species were evaluated for their total phenols, flavonoids, carotenoids contents and total antioxidant capacity assessed by 2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and 2,2- diphenyl-1- picrylhydrazyl (DPPH) assays. Total protein content ranged from 99.49 (Acacia cyclops) to 142.77 (Acacia cyanaphylla) mg/g dry weight basis (DW) according to the Acacia species, having an average of 120.41 mg/g DW. The albumin constitutes the major fraction presenting 31.11%, followed by globulin (27.36%), prolamin (22.27%) and glutelin (19.27%). The Acacia seeds were rich in minerals. The total phenolic compounds (TPC) determinate ranged from 154.47 (A. cyclops) to 1217.99 (Acacia horrida) with an average of 632.40 mg gallic acid equivalents/100 g DW. The total flavonoid contents have an average of 11.21 mg rutin equivalents/100 g DW, and the carotenoids mean is 4.23 mg/kg. The Acacia seed extracts have an antioxidant activity reflected by the ABTS and DPPH tests. The Duncan's test showed significant difference of proteinic, phenolics, flavonoid and carotenoid contents according to the Acacia species. Therefore, natural components from Acacia seeds could be incorporated as food ingredient, without causing detrimental effects to the food's palatability and the functional ingredient's efficacy. © 2011 Academic Journals.


Ghazghazi H.,Institute National Dagronomie Of Tunis | Miguel M.G.,University of Algarve | Miguel M.G.,University of Lisbon | Hasnaoui B.,Institute Sylvo Pastoral | And 5 more authors.
African Journal of Biotechnology | Year: 2010

The antioxidant activity of leaf extracts of Rosa canina from diverse localities of Tunisia were evaluated by ABTS and DPPH methods, whereas in those of essential oils and carotenoids extracts such activity was determined only by the ABTS method. Total phenols determined by the Folin method revealed that at Aindraham, samples showed a great variability of phenol content in contrast to those from Feija. After chemical analysis of the essential oils by gas chromatography (GC) and gas chromatography coupled to mass spectra (GC-MS), revealed that the oils of Feija were predominantly composed of palmitic acid, vitispirane, linoleic acid, lauric acid, myristic acid and phytol acetate, while in those samples from Aindraham predominated vitispirane, palmitic acid, linoleic acid and phytol acetate. Higher concentrations of β-carotene and lycopene were found in the samples from Aindraham after determination by high performance liquid chromatography (HPLC). All samples possess antioxidant activity, nevertheless much more significant in phenol extracts in contrast to the carotenoid extracts, which possess the lowest activity. © 2010 Academic Journals.


Nasri N.,University of Tunis | Nasri N.,Institute National Of Recherches En Genie Rural Eaux Et Forets | Elfalleh W.,Institute des Regions Arides | Tlili N.,University of Tunis | And 4 more authors.
Lipids in Health and Disease | Year: 2012

Background: Oilseed samples from four Acacia species (A. cyclops, A. ligulata, A. salicina and A. cyanophylla) were analyzed in order to evaluate the potential nutritional value of their unexploited seeds. Methods: Samples were collected from different Tunisian geographic locations. Seed oils were extracted and carotenoids, tocopherols and sterols were analyzed using chromatographic methods. Results: The studied Acacia seeds seem to be quite rich in lipids (from 6% to 12%). All Acacia species contain mainly the xanthophylls zeaxanthin and lutein compounds: from ca. 38 mg.kg-1 of total lipids (A. cyclops) to ca. 113 mg.kg-1 of total lipids (A. cyanophylla). Total tocopherols varied from ca. 221 mg.kg-1 of total lipids (A. cyclops) to ca. 808 mg.kg-1 of total lipids (A. ligulata). Sterols are highly present and their contents ranged between ca. 7 g. kg-1 of total lipids (A. salicina) and 11 g. kg-1 of total lipids (A. cyclops). Conclusion: This study highlights that these unexploited seeds might have a potential nutritional value and encourages researchers to more explore and find developments for these plants for healthy purposes. © 2012 Nasri et al.; licensee BioMed Central Ltd.


Tlili N.,Tunis el Manar University | Elfalleh W.,Institute Des Regions Arides Of Medenine | Hannachi H.,Institute Des Regions Arides Of Medenine | Yahia Y.,Institute Des Regions Arides Of Medenine | And 3 more authors.
International Journal of Food Properties | Year: 2013

Medicinal plants contain high levels of natural antioxidants and exhibited strong antioxidant activity. In order to find new sources of natural antioxidants, a screening of natural antioxidants from some Tunisian medicinal plants commonly associated with treatment of some diseases were conducted. The total phenolic compounds, flavonoids, anthocyanins, and hydrolysables tannins were measured, and the antioxidant capacities were evaluated using DPPH and Trolox equivalent antioxidant capacity assays. Results prove that there are important quantities of these compounds in Tunisian medicinal plants. A positive linear correlation between Trolox equivalent antioxidant capacity and the phenolic compound contents suggested that these compounds were a major contributor of antioxidant activity of these plants. The results from this study will help us understand the antioxidant capacity profiles of these medicinal plants, and also investigate new sources of natural antioxidants. Copyright © 2013 Taylor & Francis Group, LLC.

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