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Tabbene O.,Center de Biotechnologie de la Technopole de Borj-Cedria | Azaiez S.,Center de Biotechnologie de la Technopole de Borj-Cedria | Di Grazia A.,University of Rome La Sapienza | Karkouch I.,Center de Biotechnologie de la Technopole de Borj-Cedria | And 7 more authors.
Journal of Applied Microbiology | Year: 2016

Aims: In this study, we evaluated the ability of the lipopeptide bacillomycin D and the antifungal drug amphotericin B as well as their combination, to inhibit Candida albicans biofilm formation and to accelerate keratinocyte cell migration. Methods and Results: The antibiofilm activity of bacillomycin D and its combination with amphotericin B was carried out by crystal violet colorimetric method. Our results have shown that, when combined together at low concentrations nontoxic to mammalian cells, corresponding to 1/32 MIC (0·39 μg ml-1) and 1/4 MIC (0·06 μg ml-1) for bacillomycin D and amphotericin B, respectively, a clear antibiofilm activity is manifested (95% inhibition of biofilm formation) along with a clear inhibition of germ tube formation. Moreover, the effect of both drugs on preformed biofilm of C. albicans strain was determined using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction assay. The combination of the two antifungal compounds at 0·39 and 1 μg ml-1 for bacillomycin D and amphotericin B, respectively, resulted in a clear enhancement of biofilm eradication compared to the results obtained with each drug alone. Furthermore, this combination was found to promote the closure of a gap produced in a monolayer of human keratinocytes. Conclusions: Bacillomycin D and its combination with amphotericin B display impressive anti-biofilm and wound-healing activities. Significance and Impact of the Study: Application of the lipopeptide bacillomycin D and the antifungal drug amphotericin B in medical devices may offer a promising alternative for topical treatment of Candida-associated infections in the setting of a wound. © 2016 The Society for Applied Microbiology. Source


Tabbene O.,Center de Biotechnologie de la Technopole de Borj-Cedria | Gharbi D.,Center de Biotechnologie de la Technopole de Borj-Cedria | Slimene I.B.,Center de Biotechnologie de la Technopole de Borj-Cedria | Elkahoui S.,Center de Biotechnologie de la Technopole de Borj-Cedria | And 5 more authors.
Applied Biochemistry and Biotechnology | Year: 2012

In the present study, we evaluated the antioxidant and the scavenging ability of C14, C15 and C16 bacillomycin D-like lipopeptides produced by B38 strain. They all displayed strong reducing power activity, hydroxyl and superoxide anion radicals scavenging activities and inhibition of lipid peroxidation. In addition, they were found to protect plasmid DNA damage from hydroxyl radical oxidation. Data suggested that their antioxidant potency can be attributed to the hydrophobic and aromatic side-chain groups of their amino acids as well as to the aliphatic chain of their beta amino fatty acids. Note that the hydrocarbon chain length did not interfere with the antioxidant power. Overall, such bacillomycin D lipopeptides which exhibit antioxidant and radical scavenging activities may be useful for cosmetic, therapeutic or pharmaceutical purposes in order to delay or prevent oxidative deterioration of manufactured products. © 2012 Springer Science+Business Media New York. Source


Chabir N.,Enig Associates, Inc. | Chabir N.,Laboratoire Of Biotechnologie Appliquee A Lamelioration Des Cultures | Ben Brahim N.,Institute National Of Recherches Agronomiques | Mars M.,Laboratoire Of Biotechnologie Appliquee A Lamelioration Des Cultures | Romdhane M.,Enig Associates, Inc.
Acta Horticulturae | Year: 2010

A comparative study between hydrodistillations with and without ultrasons, was carried out for the extraction of essential oils of Melaleuca armillaris leafs cultivated in Tunisia. We studied, for each method, the yield and the chemical composition of essential oils obtained. The chromatographic analysis of essential oils by GC-MS, shows that the majority compounds are the same for both oils: 1.8- cinéole, borneol, α-pinene, ?-terpinene. The extraction under ultrasons allows a better output to be obtained in a shorter time. The extraction of essential oils of Melaleuca armillaris under ultrasons is moreover more effective than that by simple hydrodistillation. Source


Gasmi-Boubaker A.,Institute National Agronomique Of Tunisie | Selmi H.,Center Des Recherches En Grandes Culture Of Beja | Mosquera Losada R.,University of Santiago de Compostela | Ben Youssef S.,Institute National Of Recherches Agronomiques | And 3 more authors.
Livestock Research for Rural Development | Year: 2012

The potential nutritional value of four species of leguminous forage (Hedysarum coronarium L, Medicago truncatula L, Vicia sativa L, Pisum sativum L) grown in Tunisia were harvested at 50 % of flowering stage and evaluated for their nutritive value by chemical composition and gas production technique. The content of organic matter ranged from 846.7 to 946.5g/kg dry matter (DM). The forage had high crude protein (195-216 g/kg DM) and variable neutral detergent fiber (NDF: 397- 432g/kg DM) contents. All species contained satisfactory levels of phosphorus, calcium, potassium, sodium, magnesium and iron. The gas production after 48 h incubation ranged from 45.6 to 65mL/200mg DM. Metabolisable energy (ME) and short chain fatty acids (SCFA) were different among the species, with the highest value recorded for Hedysarum coronarium L and Vicia sativa L. The result indicates that such legumes have the potential to be used to feed ruminants. Source


Debbabi O.S.,Banque Nationale de Genes | Bouhlal R.,Institute National Of Recherches Agronomiques | Abdelaali N.,Institute National Of Recherches Agronomiques | Mnasri S.,Banque Nationale de Genes | Mars M.,University of Sousse
International Journal of Fruit Science | Year: 2013

The aim of this study was to characterize the major orange cultivars from Tunisia and to study the genetic diversity within this fruit tree species. This work was conducted in the framework of the activities of the fruit tree network in the Tunisian National Gene Bank. The pomological diversity of 28 Tunisian sweet orange cultivars was analyzed based on 18 qualitative and quantitative traits. Different regions of Tunisia, where orange cultivation is prominent, were investigated. Principal components analysis conducted on quantitative traits showed an important degree of variability of about 94% for the three first principal components. Factorial analysis of correspondence was conducted for qualitative data and it showed 69% of the total diversity for the three first factors. A dendrogram was constructed based on average distances. Results showed that the geographic origin was not the determinant criteria for cultivar segregation. © 2013 Copyright Taylor and Francis Group, LLC. Source

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