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Sfairi Y.,Direction Provinciale des Eaux et Forets et de la lutte contre la Desertification | Sfairi Y.,Cadi Ayyad University | Lahcen O.,Direction Provinciale des Eaux et Forets et de la lutte contre la Desertification | Al Feddy M.N.,Institute National Of Recherche Agronomique | Abbad A.,Cadi Ayyad University
African Journal of Biotechnology | Year: 2012

Atlas cypress (Cupressus atlantica Gaussen) is an endemic coniferous medicinal species geographically restricted to the N'Fis valley river in the High Atlas Mountains in South-Western Morocco. Eight dormancy-breaking treatments, 5 NaCl concentrations, and 5 water potentials were tested on the germination of Altas cypress seeds that had been stored in a cold room for 5 years after collecting from Aghbar population. Hand scarification, gibberellic acid and hot water increased the seed germination percentage (up to 75%), and mechanical scarification and gibberellic acid (1000, 2000 ppm) induced a faster speed germination. Soaking in sulfuric acid (10%) did not improve the seed germination of C. atlantica. In addition, salinity higher than 160 mM NaCl and water potential below - 0.53 MPa drastically reduced seed germination. © 2012 Academic Journals.

Mulder N.J.,University of Cape Town | Adebiyi E.,Covenant University | Alami R.,Sanguine | Benkahla A.,Institute Pasteur Of Tunis | And 31 more authors.
Genome Research | Year: 2016

The application of genomics technologies to medicine and biomedical research is increasing in popularity, made possible by new high-throughput genotyping and sequencing technologies and improved data analysis capabilities. Some of the greatest genetic diversity among humans, animals, plants, and microbiota occurs in Africa, yet genomic research outputs from the continent are limited. The Human Heredity and Health in Africa (H3Africa) initiative was established to drive the development of genomic research for human health in Africa, and through recognition of the critical role of bioinformatics in this process, spurred the establishment of H3ABioNet, a pan-African bioinformatics network for H3Africa. The limitations in bioinformatics capacity on the continent have been a major contributory factor to the lack of notable outputs in highthroughput biology research. Although pockets of high-quality bioinformatics teams have existed previously, the majority of research institutions lack experienced faculty who can train and supervise bioinformatics students. H3ABioNet aims to address this dire need, specifically in the area of human genetics and genomics, but knock-on effects are ensuring this extends to other areas of bioinformatics. Here, we describe the emergence of genomics research and the development of bioinformatics in Africa through H3ABioNet.

Rhouma A.,Institute Of Lolivier | Helali F.,Laboratoire Of Quarantaine Ministere Of Lagriculture | Chettaoui M.,Institute Of Lolivier | Hajjouji M.,Laboratoire Of Quarantaine Ministere Of Lagriculture | Hajlaoui M.R.,Institute National Of Recherche Agronomique
Plant Disease | Year: 2014

In the spring of 2012 and 2013, symptoms similar to those of fire blight were observed on pear trees (Pyrus communis cv. Alexandrine, Williams) in Tunisia at flowering stages. Disease symptoms appeared in 2012 in the region of Mornag and in the following year extended to the regions of Manouba and Tebourba. More recently, the disease was observed in the regions of Bizerte, Zaghouan, and Beja. The percentages of orchard areas that had infected plants varied from 10 to 40%. Some orchards in Mornag region exhibited more than 75% disease incidence. Symptoms were observed on flowers and young shoots. Blighted blossoms appeared wilted, shriveled, and brown, and dead flowers remained on the stems. Infected shoots wilted rapidly and often formed shepherd's crooks at their tips. Samples of diseased young shoots and flowers were subjected to pathogen isolation and identification. Bacteria were isolated from washed tissues on King's B medium (KB) (1). Colonies with morphology similar to that of Erwinia amylovora were purified by sub-culturing on KB. The strains were first characterized based on morphology and biochemical tests (1). Sixteen strains produced white colonies on KB, were gram-negative, did not produce a fluorescent pigment on KB, did not grow at 35°C, and induced a hypersensitive reaction when infiltrated into tobacco leaves (cv. Xanthi). These strains were identified as E. amylovora by double-antibody sandwich indirect-ELISA and immunofluorescene microscopy using a polyclonal antibody (2) and nested PCR targeting the pEA29 plasmid (3). Pathogenicity was tested using a detached-fruit assay (1). Each strain was inoculated onto three pear fruit (cv. Alexandrine) wounded with a scalpel dipped in a 109 CFU/ml bacterial suspension. The inoculated fruit were incubated at 25°C and 80% relative humidity in plates with sterile 1% agar. Negative controls consisted of fruit wounded with a scalpel dipped in sterile distilled water. Seven days after inoculation, symptoms of discoloration, browning, and production of bacterial ooze appeared at the inoculated points. No symptoms developed on negative controls. Reisolation of bacteria yielded colonies with characteristics of E. amylovora. Purified amplicons from nested PCR were sequenced (KF302525, KF302526) and a BLAST search of the GenBank database revealed 98% homology with E. amylovora strain HF560643.1. © The American Phytopathological Society.

Hue T.,Institute Agronomique neo Caledonien | Hurlin J.-C.,Institute Agronomique neo Caledonien | Teurlai M.,Institute Of Recherche Pour Le Developpement | Naves M.,Institute National Of Recherche Agronomique
Tropical Animal Health and Production | Year: 2014

The comparison of resistance to natural tick infestation by Rhipicephalus microplus (Canestrini, 1887) of crossbred Senepol × Limousin and purebred Limousin cattle was investigated. The Senepol breed, originated from St Croix Island in the Caribbean is considered as a Bos taurus breed adapted to tropical conditions. Despite its B. taurus genetic background, it is believed to have a good tick resistance, but this resistance has never been assessed previously. Tick counts under natural infestation were carried out to investigate the difference of susceptibility between crossbred Senepol × Limousin and purebred Limousin cattle. Mixed-effect models were used to assess the effect of the breed on the number of ticks. Results show that Senepol × Limousin are five times less infested by ticks than purebred Limousin. These results underline the opportunity to use Senepol cattle for crossing with susceptible B. taurus breeds in tick infested areas, to combine tick resistance with beef production abilities. © 2013 Springer Science+Business Media Dordrecht.

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