Quantification of methadone and its metabolite 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine in third instar larvae of lucilia sericata (Diptera: Calliphoridae) using liquid chromatography-tandem mass spectrometry
Gosselin M.,Institute National Of Criminalistique Et Of Criminologie |
Gosselin M.,University of Mons |
Fernandez M.D.M.R.,Institute National Of Criminalistique Et Of Criminologie |
Wille S.M.R.,Institute National Of Criminalistique Et Of Criminologie |
And 4 more authors.
Journal of Analytical Toxicology | Year: 2010
Entomotoxicology studies the application of toxicological analysis on necrophageous insects present on human remains. This paper describes the development and validation of a sensitive liquid chromatography-tandem mass spectrometry method for quantification of methadone and its main metabolite, 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine (EDDP), in developmental stages of Lucilia sericata. One single larva was pulverized in a disposable vial and then extracted with 1-chlorobutane. After evaporation of the organic layer, samples were reconstituted in the mobile phase. Chromatographic separation was achieved on a NUCLEODUR® Sphinx RP column with a liquid chromatographic gradient (0.1% formic acid and methanol), ensuring the elution of methadone and EDDP within 15 min. The method was fully validated according to international guidelines. The use of liquid-liquid extraction was demonstrated to be effective (matrix effect < 27% and recovery > 66%). The method was linear over the dynamic range (10-400 pg/mg larva) with excellent within- and between-run precision and bias (CV% < 5%). The lower limit of quantification was fixed at 10 pg/mg larva. No instability of the extracted samples was observed in the autosampler after three freeze/thaw cycles and after two months at -20°C. The validated method was applied to third instar larvae of Lucilia sericata reared on beef heart spiked with 4 μg/g methadone and on a postmortem methadone overdose case. The validation and actual sample analysis showed that the method is sensitive, rugged, precise, accurate, and well-suited for routine analysis of methadone and EDDP in a single larva obtained from forensic cases.
Braet Y.,Institute National Of Criminalistique Et Of Criminologie |
Bourguignon L.,Institute National Of Criminalistique Et Of Criminologie |
Vanpoucke S.,Institute National Of Criminalistique Et Of Criminologie |
Drome V.,Institute National Of Criminalistique Et Of Criminologie |
Hubrecht F.,Institute National Of Criminalistique Et Of Criminologie
Forensic Science International | Year: 2015
The calliphorid Cynomya mortuorum (L., 1761) is a forensically important species mainly found in the Palearctic region. Knowledge about its biology and ecology is scarce. Thermal constants as well as developmental time were studied at constant and variable regimes of 5 average temperatures: 14, 16, 18, 20 and 22. °C, respectively. Total developmental time varied between 15.82. ±. 0.40 days at 22. °C and 28.67. ±. 2.38 days at 14. °C, for the constant regime, and between 16.05. ±. 0.67 days at 22. °C and 32.79. ±. 1.77 days at 14. °C, for the variable regime. No significant differences were observed between ADD, and threshold at the constant (ADD: 277.39. ±. 14.78 DD; lower threshold: 4.72. °C) and variable regimes (275.99. ±. 14.16 DD; lower threshold: 5.05. °C). © 2015 Elsevier Ireland Ltd.
PubMed | Institute National Of Criminalistique Et Of Criminologie
Type: | Journal: Biodiversity data journal | Year: 2015
The calliphorid Cynomyamortuorum (L., 1761) is a species of forensic interest, present mainly in the Palaearctic Region. Nearly nothing is known about its life history.We provide here the first data regarding pupal weight evolution during the pupal stage, female fertility and life expectancy of the species. At 22C under a variable regime of temperatures, the egg-to-adult development time was an average of 18.05 0.72 and 18.47 0.67 days for females and males, respectively, in the control group. The pupal stage represented 56.7% of the total development. The development time from egg to adult and the duration of the pupal stage were significantly longer for males than for females. The measurement of pupal weight at the start of the pupal period revealed that female pupae were significantly lighter than male pupae by nearly 20%. This difference between the sexes was also observed for the dry weight of adults. An average decrease of 8.75% was observed throughout the first 8 days of the pupal stage, after which most adults started to emerge. The tested females produced an average of 176.13 66.62 eggs throughout the egg-laying period. The average lifespan after emergence was 12.10 4.09 days for females and 12.60 2.95 days for males, with a median of 12.50 days for both sexes.