Institute National Des Science Applique Es Et Of Technology

Tunis, Tunisia

Institute National Des Science Applique Es Et Of Technology

Tunis, Tunisia
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Bouanane-Darenfed A.,University of Science and Technology Houari Boumediene | Hania W.B.,Aix - Marseille University | Hania W.B.,Institute National Des Science Applique Es Et Of Technology | Hacene H.,University of Science and Technology Houari Boumediene | And 3 more authors.
International Journal of Systematic and Evolutionary Microbiology | Year: 2013

A hyperthermophilic anaerobic bacterium, designated D2C22T, was isolated from the hydrothermal hot spring of Guelma in north-east Algeria. The isolate was a Gram-stain-positive, non-sporulating, non-motile rod, appearing singly or in pairs (0.3-0.4×8.0-9.0 mm). Strain D2C22T grew anaerobically at 45-85 °C (optimum 65 °C), at pH 5-9 (optimum pH 6.8) and with 0-20 g NaCl l-1. Strain D2C22T used glucose, galactose, lactose, fructose, ribose, xylose, arabinose, maltose, cellobiose, mannose, melibiose, sucrose, xylan and pyruvate (only in the presence of yeast extract or biotrypticase) as electron donors. The end products from glucose fermentation were acetate, lactate, CO2 and H2. Nitrate, nitrite, thiosulfate, elemental sulfur, sulfate and sulfite were not used as electron acceptors. The predominant cellular fatty acids were iso-C15: 0 and iso-C17: 0. The DNA G+C content was 41.6 mol%. Phylogenetic analysis of the 16S rRNA gene sequence indicated that strain D2C22T was most closely related to Caldicoprobacter oshimai JW/HY-331T, Caldicoprobacter algeriensis TH7C1T and Acetomicrobium faecale DSM 20678T (95.5, 95.5 and 95.3% 16S rRNA gene sequence similarity, respectively). Based on phenotypic, phylogenetic and chemotaxonomic characteristics, strain D2C22T is proposed to be a representative of a novel species of the genus Caldicoprobacter within the order Clostridiales, for which the name Caldicoprobacter guelmensis sp. nov. is proposed. The type strain is D2C22T (=DSM 24605T=JCM 17646T). © 2013 IUMS.


Fadhlaoui K.,Aix - Marseille University | Fadhlaoui K.,Institute National Des Science Applique Es Et Of Technology | Hania W.B.,Aix - Marseille University | Hania W.B.,Institute National Des Science Applique Es Et Of Technology | And 5 more authors.
International Journal of Systematic and Evolutionary Microbiology | Year: 2015

A novel anaerobic, mesophilic, slightly halophilic sulfate-reducing bacterium, designated strain Khaled BD4T, was isolated from waters of a Tunisian thermal spring. Cells were vibrio-shaped or sigmoids (5–7×1–1.5µm) and occurred singly or in pairs. Strain Khaled BD4T was Gram-stainnegative, motile and non-sporulated. It grew at 25–45 °C (optimum 37 °C), at pH 5.5–8.3 (optimum pH 7.0) and with 0.5–8% NaCl (optimum 3%). It required vitamins or yeast extract for growth. Sulfate, thiosulfate, sulfite and elemental sulfur served as terminal electron acceptors, but not fumarate, nitrate or nitrite. Strain Khaled BD4T utilized H2 in the presence of 2 mM acetate (carbon source), but also lactate, formate, pyruvate and fumarate in the presence of sulfate. Lactate was incompletely oxidized to acetate. Amongst substrates used, only pyruvate was fermented. Desulfoviridin and c-type cytochrome were present. The G+C content of the DNA was 54.6 mol%. The main fatty acids were anteiso-C15: 0, iso-C18: 0, iso-C17: 0 and iso-C14: 0. Phylogenetic analysis of the 16S rRNA gene sequence indicated that strain Khaled BD4T had Desulfovibrio giganteus DSM 4123T (96.7% similarity) as its closest phylogenetic relative. On the basis of 16S rRNA gene sequence comparisons together with genetic and physiological characteristics, strain Khaled BD4T is assigned to a novel bacterial species, for which the name Desulfovibrio biadhensis sp. nov. is proposed. The type strain is Khaled BD4T (=DSM 28904T=JCM 30146T). © 2015 IUMS.

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