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Medini H.,University of Monastir | Elaissi A.,University of Monastir | Khouja M.L.,Institute National des Recherches en Genie Rural | Piras A.,University of Cagliari | And 4 more authors.
Natural Product Research | Year: 2011

This study is designed to examine the chemical composition and antioxidant activity of the essential oil of Juniperus phoenicea L. ripe and unripe berries. GC and GC/MS analyses resulted in the detection of 42 components representing approximately 96.50-99.57% of the oils. Major components of the oils were-pinene (58.61-77.39%), camphene (0.67-9.31%), δ-3-carene (0-10.01%) and trans-verbenol (0-5.24%). Antioxidant activities were determined by two different test systems, DPPH and ABTS radical scavenging activities. In both systems ripe berries exhibited better activity potential than the unripe ones. © 2011 Taylor & Francis.

Gasmi-Boubaker A.,Institute National Agronomique Of Tunisie | Salah F.B.,Institute National Agronomique Of Tunisie | Hassen H.,Institute National Agronomique Of Tunisie | Bergaoui R.,Institute National Agronomique Of Tunisie | Khaldi A.,Institute National des Recherches en Genie Rural
EAAP Scientific Series | Year: 2011

An important objective in livestock feeding is to promote the use of local feeds and by-products in order to reduce feeding costs. Carob pulp had been frequently used for feeding ruminants in the Mediterranean areas. This study aimed to evaluate carob pulp as an alternative dietary energy source to barley in a diet for growing rabbits. Three dietary treatments designated as D0 (commercial feed without carob pulp), D8 (commercial feed incorporating 8% carob pulp) and D15 (commercial feed incorporating 15% carob pulp) were fed to 60 rabbits (20 rabbits per group) weaned at 28 d of age to evaluate growth performance (from 29 to 70 days of age). At the end of the growth trial, 10 rabbits were slaughtered in each group, and used for the evaluation of carcass traits. Another 15 rabbits (5 rabbits per group; 2,100±200 g) were used in a digestibility trial to determine digestibility coefficient of diets. No significant effect was detected on the final weight and the daily gain. The feed intake was higher in rabbits fed the commercial diet (D0) than in other rabbits (123.3, 109.5 and 119.1 g/d in rabbits fed the D0, D8 and D15, respectively; P<0.05). Similarly, feed conversion tended to be higher (3.33, 3.05 and 3.03 kg/kg in rabbits fed D0, D8 and D15, respectively; P<0.05), while dressing out percentage was significantly lower (57.67, 59.08 and 59.89% in rabbits fed D0, D8 and D15, respectively; P<0.05) in rabbits fed the commercial diet. There were small significant differences in digestibility of energy and organic matter between the 3 experimental diets. It can be concluded from the finding that incorporation of carob pulp at 15% inclusion level can replace barley grain as an energy source for growing rabbits.

Hanene M.,University of Monastir | Ameur E.,University of Monastir | Larbi K.M.,Institute National des Recherches en Genie Rural | Piras A.,University of Cagliari | And 5 more authors.
Natural Product Research | Year: 2012

This study is outlined to probe the chemical composition of essential oil and in vitro antioxidant activity of Juniperus oxycedrus ssp. macrocarpa (S. & m.) Ball. and Juniperus oxycedrus L. ssp. rufescens (L. K.) berries, collected from four sites, according to their maturity phase. The chemical composition of the hydrodistilled essential oil was analysed by GC-MS. Forty-eight compounds were identified, accounting for approximately 79.8-98.9% of the oil. The main constituents were α-pinene, germacrene D, myrcene, abietadiene and cis-calamenene, their mean percentage vary according to their phenological stage. The antioxidant activity of the samples was determined by the ABTS and DPPH radical scavenging activities. Hawaria essential oil extracted from mature berries showed the highest antioxidant capacity. © 2011 Taylor & Francis.

Medini H.,University of Monastir | Elaissi A.,University of Monastir | Khouja M.L.,Institute National des Recherches en Genie Rural | Chraief I.,University of Monastir | And 4 more authors.
Chemistry and Biodiversity | Year: 2010

The essential oil composition of leaves of 60 individual trees of Juniperus oxycedrus L. growing in four locations in Tunisia harvested in three different seasons were investigated by GC and GC/MS. Seventy compounds were identified in the oils, and a relatively high variation in their contents were found. All the oils were dominated by terpenic hydrocarbons, with a-pinene (27.35-58.03%) as the main component, followed by geranyl acetone (13; 1.96-7.14%), 13-epimanoyl oxide (16; 1.35-6.95%), and eudesma-4(15),7-dien-1-ol (11; 1.39-4.18%). The 18 major oil components were processed by hierarchical clustering and principal component analysis (PCA) allowing to establish four groups, one divided into two subgroups, of populations according to the location and season of harvest. Their oils were differentiated by one compound or more, showing a clear seasonal and geographical polymorphism in their chemical composition allowing the identification of specific chemotypes. The pattern of geographic variation in the essential oil composition indicated that the oils of the populations from the continental site (Makthar) were clearly distinguished from those of the littoral localities (Tabarka, Hawaria, and Rimel). © 2010 Verlag Helvetica Chimica Acta AG.

Elaloui M.,Institute National des Recherches en Genie Rural | Laamouri A.,Institute National des Recherches en Genie Rural | Fabre J.,ENSIACET | Mathieu C.,ENSIACET | And 2 more authors.
Natural Product Research | Year: 2015

Ziziphus jujuba pulps are very much appreciated by the inhabitants and have been recently exported. This article reports on the chemical composition (amino acids, polyphenols and sugars) of the pulps of four Z. jujuba ecotypes (Choutrana, Mahdia, Mahres and Sfax). The major amino acids identified were proline, aspartic acid and glutamic acid. Among these, proline was the most abundant amino acid (17.4 mol). Considerable differences in total phenolic contents (15.85 mg/L) were found. Predominant phenols identified by using HPLC were rutin (1.09 mg/L) and chlorogenic acid (2.57 mg/100 g). Sugars isolated from Ziziphus pulps were found at a rate of 43.52%. Using HPLC method, three sugars from the pulp extract were identified: glucose, galactose and sucrose. The Mahdia ecotype was the richest in these sugars with 0.45, 136.51 and 113.28 mg/L, respectively. © 2015 Taylor & Francis.

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