Institute National des Recherches en Genie Rural

Ariana, Tunisia

Institute National des Recherches en Genie Rural

Ariana, Tunisia
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Elaloui M.,Institute National des Recherches en Genie Rural | Ghazghazi H.,Institute National des Recherches en Genie Rural | Ennajah A.,Institute National des Recherches en Genie Rural | Manaa S.,University of Tunis | And 3 more authors.
Natural Product Research | Year: 2016

The aim of this work was to evaluate some secondary metabolites, antioxidant activity of methanolic leaf extracts of five Ziziphus spina-christi provenances (INRGREF, Tozeur, Degueche, Nafta and Kebelli) and their allelopathic effects on Trigonella foenum-graecum and Lens culinaris. Leaves were collected during 2013 and 2014. Total phenols, flavonoids, tannins and antioxidant activity were evaluated using the Folin ciocalteux, Aluminum trichloride, vanillin and scavenging activity on 22-diphenyl-1-picrylhydrazyl (DPPH) radical methods, respectively. Total phenols, tannins and flavonoids were present, at levels of 57.41 mg GAE/g DW, 31.98 mg RE/g DW and 14.68 μg CE/g DW, respectively. The high antioxidant activity (0.086 μg/mL) was noted in kebelli provenance (2013). The highest germination, plumule and radicle lengths of tested species were observed in INRGREF provenance. Z. spina-christi leaf extracts may be suggested in foods and pharmaceutical industries. Leaf extracts could also provide a natural herbicide with a positive impact on the environment. © 2016 Informa UK Limited, trading as Taylor & Francis Group

Ghrab M.,Institute Of Lolivier | Zitouna R.,Institute National des Recherches en Genie Rural | Masmoudi M.M.,Institute National Agronomique Of Tunisie | Mechlia N.B.,Institute National Agronomique Of Tunisie
International Journal of Fruit Science | Year: 2016

Productivity of peach cultivars with different ripening seasons was studied under the growers’ conventional conditions of Tunisia. Experiments were performed in northern Tunisia (36° 41ʹ N, 10° 15ʹ E). Performances of Flordastar, Royal Glory, and Carnival as respectively early, mid-season, and late ripening cultivars were evaluated under the grower’s conventional irrigation programs. Fruit growth, yield, water supply, and water productivity were determined through four years. For the three cultivars, Flordastar, Royal Glory, and Carnival, fruit maturity was achieved at 650, 1100, and 2200 GDD, respectively. Average yields were 17.4, 29.8, and 41.1 t ha–1 and mean values of water productivity were 1.9, 3.1, and 3.6 kg fresh yield m–3 for early, mid-season, and late cultivars, respectively. Results of agronomic performances evaluation at the farm scale provided a reference on the behavior of early, mid-season, and late cultivars of peach in irrigated orchards in semi-arid conditions. © 2016 Taylor & Francis

Elaloui M.,Institute National des Recherches en Genie Rural | Laamouri A.,Institute National des Recherches en Genie Rural | Fabre J.,ENSIACET | Mathieu C.,ENSIACET | And 2 more authors.
Natural Product Research | Year: 2015

Ziziphus jujuba pulps are very much appreciated by the inhabitants and have been recently exported. This article reports on the chemical composition (amino acids, polyphenols and sugars) of the pulps of four Z. jujuba ecotypes (Choutrana, Mahdia, Mahres and Sfax). The major amino acids identified were proline, aspartic acid and glutamic acid. Among these, proline was the most abundant amino acid (17.4 mol). Considerable differences in total phenolic contents (15.85 mg/L) were found. Predominant phenols identified by using HPLC were rutin (1.09 mg/L) and chlorogenic acid (2.57 mg/100 g). Sugars isolated from Ziziphus pulps were found at a rate of 43.52%. Using HPLC method, three sugars from the pulp extract were identified: glucose, galactose and sucrose. The Mahdia ecotype was the richest in these sugars with 0.45, 136.51 and 113.28 mg/L, respectively. © 2015 Taylor & Francis.

Elaloui M.,Institute National des Recherches en Genie Rural | Laamouri A.,Institute National des Recherches en Genie Rural | Albouchi A.,Institute National des Recherches en Genie Rural | Cerny M.,ENSIACET | And 3 more authors.
Emirates Journal of Food and Agriculture | Year: 2014

Thirteen fatty acids were identified from the pulps of four Tunisian Ziziphus jujuba ecotypes (Sfax, Choutrana, Mahres and Mahdia), using capillary gaseous chromatography method. These oils presented 8.31% to 12.35% of dry weights. Compared to the other ecotypes, Mahres and Choutrana were the richest of the oleic acid. The palmitic acid was the most important compound of the Sfax ecotype. Unsaturated fatty acids ranged from 62.63% to 72.40% of the total fatty acids of each ecotype. So a ratio of the unsaturated/saturated (U/S) varied from 1.68 to 2.37. The β-sitosterol and the Stigmasterol were identified as major sterols. The β-sitosterol was the prominent component in the all ecotypes and the highest level (10.65 mg/100g) was noted in Choutrana ecotype. Stigmasterol was more abundant in Sfax (16.12 mg/100g) and Choutrana (4.67 mg/100g) ecotypes. The campesterol sterol was identified only in Sfax and Choutrana ecotypes being more important (2.4 mg/100g) in Choutrana pulps.

Gasmi-Boubaker A.,Institute National Agronomique Of Tunisie | Salah F.B.,Institute National Agronomique Of Tunisie | Hassen H.,Institute National Agronomique Of Tunisie | Bergaoui R.,Institute National Agronomique Of Tunisie | Khaldi A.,Institute National des Recherches en Genie Rural
EAAP Scientific Series | Year: 2011

An important objective in livestock feeding is to promote the use of local feeds and by-products in order to reduce feeding costs. Carob pulp had been frequently used for feeding ruminants in the Mediterranean areas. This study aimed to evaluate carob pulp as an alternative dietary energy source to barley in a diet for growing rabbits. Three dietary treatments designated as D0 (commercial feed without carob pulp), D8 (commercial feed incorporating 8% carob pulp) and D15 (commercial feed incorporating 15% carob pulp) were fed to 60 rabbits (20 rabbits per group) weaned at 28 d of age to evaluate growth performance (from 29 to 70 days of age). At the end of the growth trial, 10 rabbits were slaughtered in each group, and used for the evaluation of carcass traits. Another 15 rabbits (5 rabbits per group; 2,100±200 g) were used in a digestibility trial to determine digestibility coefficient of diets. No significant effect was detected on the final weight and the daily gain. The feed intake was higher in rabbits fed the commercial diet (D0) than in other rabbits (123.3, 109.5 and 119.1 g/d in rabbits fed the D0, D8 and D15, respectively; P<0.05). Similarly, feed conversion tended to be higher (3.33, 3.05 and 3.03 kg/kg in rabbits fed D0, D8 and D15, respectively; P<0.05), while dressing out percentage was significantly lower (57.67, 59.08 and 59.89% in rabbits fed D0, D8 and D15, respectively; P<0.05) in rabbits fed the commercial diet. There were small significant differences in digestibility of energy and organic matter between the 3 experimental diets. It can be concluded from the finding that incorporation of carob pulp at 15% inclusion level can replace barley grain as an energy source for growing rabbits.

Jaouadi W.,Institute National des Recherches en Genie Rural | Mechergul K.,Institute National des Recherches en Genie Rural | Gader G.,Direction Generale des Forets | Khouja M.L.,Institute National des Recherches en Genie Rural
Revue d'Ecologie (La Terre et la Vie) | Year: 2013

The present study was conducted in the Bouhcdma national park and its surroundings, located in the arid area of Tunisia and considered a Biosphere reserve by UNESCO in 1977; it is a protected area with special ecological characteristics and an important dynamics of space occupation.Thc park contains the only wooded steppe with Acacia tortilis (Forsk.) Hayne subsp raddiana (Savi.) Brcnan var. raddiana Brcnan in Tunisia. With the aim of safeguarding and management of the park, a better knowledge of the distribution patterns and dynamics of the vegetation of this zone makes it possible to consider programmes of restoration and sustainable management of the natural environment. Based on the interpretation of remote sensing data and use of SIG, the study was devoted to the elaboration of charts relating to the ecological systems present in 2005, and to the distribution of the steppe with Acacia tortilis according to physical factors such as slope, altitude, type and depth of the soil. Also, the production of seeds was studied to explain the effect of physical factors on the regeneration of the species. The analysis of soil occupation dynamics translates into a good distribution (7056 ha) of steppe with Acacia tortilis. The study shows that Acacia tortilis is located on 6242 ha at an altitude of 20 m to 150 m and on 814 ha at an altitude from 150 to 250 m. This species grows on 5815 ha with a weak slope (0 to 3 %), on 928 ha with a slope of 3 to 5 % and only on 313 ha with a slope > 5 %. The results show that the Acacia tortilis is regenerated on the not very advanced soil of hydrous contribution with a surface of 3832 ha, on rendzina with 1280 ha, the isohumic soil with a surface of 986 ha. Acacia tortilis colonizes the far from deep soil with a surface of 2796 ha, the fairly deep soil with a surface of 2175, the very deep soil with a surface of 1970 ha, the not very deep soil with a surface of 75 ha and the deep soil with a surface of 40 ha.

Medini H.,University of Monastir | Elaissi A.,University of Monastir | Khouja M.L.,Institute National des Recherches en Genie Rural | Piras A.,University of Cagliari | And 4 more authors.
Natural Product Research | Year: 2011

This study is designed to examine the chemical composition and antioxidant activity of the essential oil of Juniperus phoenicea L. ripe and unripe berries. GC and GC/MS analyses resulted in the detection of 42 components representing approximately 96.50-99.57% of the oils. Major components of the oils were-pinene (58.61-77.39%), camphene (0.67-9.31%), δ-3-carene (0-10.01%) and trans-verbenol (0-5.24%). Antioxidant activities were determined by two different test systems, DPPH and ABTS radical scavenging activities. In both systems ripe berries exhibited better activity potential than the unripe ones. © 2011 Taylor & Francis.

Hanene M.,University of Monastir | Ameur E.,University of Monastir | Larbi K.M.,Institute National des Recherches en Genie Rural | Piras A.,University of Cagliari | And 5 more authors.
Natural Product Research | Year: 2012

This study is outlined to probe the chemical composition of essential oil and in vitro antioxidant activity of Juniperus oxycedrus ssp. macrocarpa (S. & m.) Ball. and Juniperus oxycedrus L. ssp. rufescens (L. K.) berries, collected from four sites, according to their maturity phase. The chemical composition of the hydrodistilled essential oil was analysed by GC-MS. Forty-eight compounds were identified, accounting for approximately 79.8-98.9% of the oil. The main constituents were α-pinene, germacrene D, myrcene, abietadiene and cis-calamenene, their mean percentage vary according to their phenological stage. The antioxidant activity of the samples was determined by the ABTS and DPPH radical scavenging activities. Hawaria essential oil extracted from mature berries showed the highest antioxidant capacity. © 2011 Taylor & Francis.

Medini H.,University of Monastir | Elaissi A.,University of Monastir | Khouja M.L.,Institute National des Recherches en Genie Rural | Chraief I.,University of Monastir | And 4 more authors.
Chemistry and Biodiversity | Year: 2010

The essential oil composition of leaves of 60 individual trees of Juniperus oxycedrus L. growing in four locations in Tunisia harvested in three different seasons were investigated by GC and GC/MS. Seventy compounds were identified in the oils, and a relatively high variation in their contents were found. All the oils were dominated by terpenic hydrocarbons, with a-pinene (27.35-58.03%) as the main component, followed by geranyl acetone (13; 1.96-7.14%), 13-epimanoyl oxide (16; 1.35-6.95%), and eudesma-4(15),7-dien-1-ol (11; 1.39-4.18%). The 18 major oil components were processed by hierarchical clustering and principal component analysis (PCA) allowing to establish four groups, one divided into two subgroups, of populations according to the location and season of harvest. Their oils were differentiated by one compound or more, showing a clear seasonal and geographical polymorphism in their chemical composition allowing the identification of specific chemotypes. The pattern of geographic variation in the essential oil composition indicated that the oils of the populations from the continental site (Makthar) were clearly distinguished from those of the littoral localities (Tabarka, Hawaria, and Rimel). © 2010 Verlag Helvetica Chimica Acta AG.

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