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Alvarez B.,Institute Nacional Of Invest Y Tecnologia Agraria Y Alimentaria Inia | Martinez P.,Institute Nacional Of Invest Y Tecnologia Agraria Y Alimentaria Inia | Yuste M.,Institute Nacional Of Invest Y Tecnologia Agraria Y Alimentaria Inia | Poderoso T.,Institute Nacional Of Invest Y Tecnologia Agraria Y Alimentaria Inia | And 4 more authors.
Developmental and Comparative Immunology | Year: 2014

Secondary lymphoid organ macrophages are involved in the establishment of innate and acquired immunity. Here, we have isolated and characterized porcine lymph node and spleen CD169+ and spleen CD163+ macrophages. Lymph node and spleen CD169+ macrophages can be both identified as CD172a+SLA-DRhiCD80/86hiCD14intTLR2+TLR4+. On the other side, spleen CD163+ macrophages are CD172a+SLA-DRintCD80/86intCD14-/loTLR2intTLR4int. In addition, these macrophages can be subdivided based on the expression of CD11R1 or CD11R3. Lymph node CD169+ macrophages phagocytozed polystyrene microspheres more efficiently than spleen CD163+ and CD169+ macrophages. All macrophages exhibited low capacity to take up and process the soluble antigen DQ-OVA. Finally, spleen CD163+ macrophages displayed the highest ability to present lysozyme to CD4+ T cells in a secondary in vitro response, followed by lymph node and spleen CD169+ macrophages. © 2013 Elsevier Ltd. Source

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