Institute Mountain and Sea

Makarska, Croatia

Institute Mountain and Sea

Makarska, Croatia

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Vidic D.,University of Sarajevo | Cavar S.,University of Sarajevo | Cavar S.,University of Ljubljana | Solic M.E.,Institute Mountain and Sea | Maksimovic M.,University of Sarajevo
Natural Product Communications | Year: 2010

Hydrodistilled essential oil and the corresponding headspace volatiles of Bosnian wild growing Thymus praecox ssp. polytrichus (A. Kern. Ex Borbàs) Jalas and Thymus praecox ssp. skorpilii (Velen.) Jalas were subjected to capillary GC-MS analysis. This work presents a detailed essential oil analysis of these two rare Thymus subspecies from Bosnia, as well as the very first report on their headspace composition. Eighty-seven volatile constituents were identified in four samples. Two alcohols were the major constituents in the essential oil of T. praecox ssp. polytrichus, a monoterpene, linalool (13.9%), and a sesquiterpene, (E)-nerolidol (10.4%), while linalyl acetate (36.7%) and linalool (22.7%) were the most abundant volatiles in the corresponding headspace sample. Oxygenated monoterpenes (57.5%) predominate in the essential oil of T. praecox ssp. skorpilii with linalyl acetate (28.7%) and linalool (14.4%) as the main representatives. Headspace sample of this subspecies also showed richness in linalyl acetate (52.4%), while the second most abundant compound was α-pinene (14.5%), a monoterpene hydrocarbon.


Avar S.,University of Sarajevo | Avar S.,University of Ljubljana | Maksimovic M.,University of Sarajevo | Vidic D.,University of Sarajevo | Solic M.E.,Institute Mountain and Sea
Pharmaceutical Biology | Year: 2010

Stachys menthifolia Vis. (Lamiaceae) is an endemic species from the Balkan Peninsula spread throughout Albania, Greece, Montenegro, and Croatia. This article presents the first investigation of the essential oil composition of this species from Croatia. Aerial parts of the plant were collected from three different natural habitats in the region of Biokovo Mountain. The studied populations showed similarity in qualitative, but not in quantitative, composition of their essential oils. Hydrodistilled volatile oil obtained from the plant material of S. menthifolia was subjected to gas chromatographic analysis coupled to mass spectrometry. More than 100 compounds were identified in the three samples, representing 86.890.8% of the total oil. The terpene profile of S. menthifolia is characterized by a high content of oxygenated sesquiterpenes (48.458.9%) and diterpene hydrocarbons (3.525.2%), with 8-α-acetoxyelemol (6.921.3%), abietatriene (3.521.1%), and 4′-methoxyacetophenone (4.517.0%) as the main constituents. © 2010 Informa UK Ltd.


Valles J.,University of Barcelona | Basic N.,University of Sarajevo | Bogunic F.,University of Sarajevo | Bourge M.,French National Center for Scientific Research | And 7 more authors.
Botanica Serbica | Year: 2014

The first assessments, done by flow cytometry, of nuclear DNA amount for five genera and 30 species of angiosperms (three monocots, 27 eudicots) from the western Balkan Peninsula, including eight taxa with some degree of endemism to this area, are presented here. These data complement the substantial existing information on plant genome size in this region, now accounting for 670 species and subspecies studied for this character. © 2014 Institute of Botany and Botanical Garden Jevremovac, Belgrade.


Fridlender A.,Aix - Marseille University | Pustahija F.,University of Sarajevo | Solic M.E.,Institute Mountain and Sea | Abadzic S.,National Museum of Bosnia and Herzegovina | And 4 more authors.
Botanica Serbica | Year: 2014

It is difficult to distinguish hysteranthous Colchicum species growing in Dalmatia and Bosnia and Herzegovina, even the two most common taxa (C. autumnale and C. neapolitanum subsp. visianii). There are no clear vegetative characteristics suitable for good identification of these species. The nuclear DNA values, however, reveal differences between these two species and also among other taxa. Genome size was studied for the first time in: C. hungaricum Janka (2C = 5.38 ± 0.3 pg); two Dalmatian endemics C. neapolitanum (Ten.) Ten. subsp. kochii (Parl.) Fridl. (2C = 10.9 ± 0.3 pg) and C. neapolitanum (Ten.) Ten. subsp. visianii (Parl.) Fridl. (2C = 10.41 ± 0.32 pg) and for Italian C. neapolitanum (Ten.) Ten. subsp. neapolitanum (2C = 10.0 ± 0.1 pg) endemic to Basilicata - Campania. Genome size of C. autumnale L. from Bosnia and Herzegovina (2C = 6.17 ± 0.31 pg) does not differ from other west European populations. Based on this important character and also on morphological studies, we propose taxonomic revision of some taxa. As genome sizes of the two generally confused species C. neapolitanum subsp. visianii and C. autumnale are very different, flow cytometry is useful for identification, conservation inventories and management concerning these two taxa. © 2014 Institute of Botany and Botanical Garden Jevremovac, Belgrade.


Siljak-Yakovlev S.,University Paris - Sud | Siljak-Yakovlev S.,University of Sarajevo | Pustahija F.,University Paris - Sud | Pustahija F.,University of Sarajevo | And 6 more authors.
Advanced Science Letters | Year: 2010

Nuclear DNA content or genome size is an important character in evaluation of biodiversity. The presence of different biogeographic plant groups, such as the remnants of Tertiary relictual, glacial and postglacial flora, makes the Balkans region a natural laboratory for evolutionary studies for many plant groups. Given the paucity of information about genome size for the Balkan flora we have determined the DNA content of 343 taxa, of which 242 values are novel, mainly from Bosnia and Herzegovina, Croatia, FYR Macedonia, Monte-Negro and Serbia. Generally, the DNA 2C-values have been estimated in several populations, and if these results were similar only one population is presented. In several taxa the presence of B chromosomes, polyploidy and hybridization events led to significant genome size variation at intraspecific level. In these cases the values of several populations are presented. The 2C DNA content and base composition (GC%) were assessed by flow cytometry and chromosome number was determined using standard methods. Genome size of studied species ranged from 1C = 0.14 pg for Selaginella helvetica to 48.00 pg for Fritillaria gracilis. Using Leitch's criteria 49% of these taxa belong to the group of very small C-values, 24% to small, 19% to medium values, 6.7% to large, and 1.2% to very large genome size. Typically, the last two groups consist of gymnosperm and monocot species. These are the first 2C-values for one family, 53 genera (9 monocots and 44 eudicots) and 242 taxa at specific (210), intraspecific (27) and hybrid (5) levels. © 2010 American Scientific Publishers.


Cavar Zeljkovic S.,Palacky University | Solic M.E.,Institute Mountain and Sea | Maksimovic M.,University of Sarajevo
Natural Product Research | Year: 2015

Helichrysum italicum (Roth) G. Don is a flowering plant of the family Asteraceae. It is rich in oil that is used for different medicinal purposes and in fragrance industry. Volatile profile of four populations of H. italicum, collected from natural habitat in Dalmatia (Croatia), was analysed by capillary GC-MS. Sample from BraČ Island had α-trans-bergamotene (10.2%) and β-acoradiene (10.1%) as the majors, whereas sample collected on Biokovo Mt. was rich in neryl acetate (8.1%). β-Acoradiene was also the main constituent of sample collected near Tijarica, whereas rosifoliol (8.5%) was the most abundant constituent in sample collected near Makarska. Presented results show the influence of environmental conditions on chemical differentiation of the volatiles of H. italicum from Croatia. © 2015 Taylor and Francis.


Sanchez-Jimenez I.,Institute Botanic Of Barcelona Ibb Csic Icub | Hidalgo O.,Ohio University | Hidalgo O.,University of Barcelona | Canela M.A.,University of Navarra | And 4 more authors.
Plant Systematics and Evolution | Year: 2012

This work focuses on the representatives of genus Echinops (Asteraceae, Cardueae) in the Aegean and Balkan regions, from the perspective of their genome evolution. Chromosome numbers were determined by orcein staining in 14 populations of nine taxa, and DNA contents were assessed by flow cytometry in 24 populations of nine taxa. A molecular phylogeny based on the internal transcribed spacer (ITS) and trnL-trnF and including first sequences for two taxa (Echinops sphaerocephalus subsp. taygeteus and E. spinosissimus subsp. neumayeri) provided a framework for discussing genome changes. From a methodological point of view, similar C-DNA value estimates were obtained when measuring, for a same population, fresh leaves from adult plants collected in the field and from cultivated seedlings. Conversely, despite giving the appearance of being correct (e. g., low coefficient of variation), genome size assessed using silica gel-preserved material differs significantly from values obtained for the same populations with fresh material. Nevertheless, silica gel-preserved material may still provide rough estimates of genome size for, e. g., inferring ploidy level. Suitable-non-silica gel-based-DNA amounts assessed for 23 populations range from 2C = 6. 52 pg (E. spinosissimus subsp. neumayeri) to 2C = 9.37 pg (E. bannaticus). Chromosome counts were established for the first time for Echinops graecus (2n = 32), E. sphaerocephalus subsp. albidus (2n = 32), E. sphaerocephalus subsp. taygeteus (2n = ca. 30), and E. spinosissimus subsp. neumayeri (2n = 28). Genome size and chromosome number are confirmed as crucial parameters for deciphering lineage diversification within the genus Echinops. © 2012 Springer-Verlag.

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