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Souza G.M.,University of Sao Paulo | Berges H.,French National Institute for Agricultural Research | Bocs S.,CIRAD - Agricultural Research for Development | Casu R.,CSIRO | And 8 more authors.
Tropical Plant Biology | Year: 2011

Sugarcane cultivars derive from interspecific hybrids obtained by crossing Saccharum officinarum and Saccharum spontaneum and provide feedstock used worldwide for sugar and biofuel production. The importance of sugarcane as a bioenergy feedstock has increased interest in the generation of new cultivars optimised for energy production. Cultivar improvement has relied largely on traditional breeding methods, which may be limited by the complexity of inheritance in interspecific polyploid hybrids, and the time-consuming process of selection of plants with desired agronomic traits. In this sense, molecular genetics can assist in the process of developing improved cultivars by generating molecular markers that can be used in the breeding process or by introducing new genes into the sugarcane genome. For meeting each of these, and additional goals, biotechnologists would benefit from a reference genome sequence of a sugarcane cultivar. The sugarcane genome poses challenges that have not been addressed in any prior sequencing project, due to its highly polyploid and aneuploid genome structure with a complete set of homeologous genes predicted to range from 10 to 12 copies (alleles) and to include representatives from each of two different species. Although sugarcane's monoploid genome is about 1 Gb, its highly polymorphic nature represents another significant challenge for obtaining a genuine assembled monoploid genome. With a rich resource of expressed-sequence tag (EST) data in the public domain, the present article describes tools and strategies that may aid in the generation of a reference genome sequence. © 2011 Springer Science+Business Media, LLC.


De Souza A.L.M.,Federal University of Fluminense | De Mesquita E.D.F.M.,FV | Franco R.M.,FV | Fonseca A.B.M.,Institute Matematica e Estatistica | Calixto F.A.A.,Federal University of Fluminense
Revista Brasileira de Medicina Veterinaria | Year: 2015

Consumers are looking for products with quality and diversity. It is extremely important to promote quality control of food concerning to its microbiological characteristics and offering safe products to the population. In Rio de Janeiro state, a short-term shelf life product as fish is highly bought and consumed. And this group is the shark fillet, especially of the species Isurus oxyrinchus (Elasmobranchii: Lamnidae), popularly known as Shortfin Mako Shark. The meat of this type of shark is a distinguished and accepted product and has relatively low cost. That is why it's in the 5th place of the ranking among the imported fishes sold in Rio de Janeiro state supermarkets. Nowadays there is no legislation that can promote an identity standard and a quality of this product. The sensory analysis (test of acceptability and preference) of 17 samples of shark fresh fillet from markets of Niterói city was carried out, and also the intentions of purchasing the product samples by the panels. The sample number 7 had the highest overall average (6.02) and the vast majority of the shark fillet was accepted by the panels, confirming its consumer preference and commercialization in Niterói city, Rio de Janeiro. The objective of this work was to analyse the sensory evaluation of Shortfin Mako Shark meat (I. oxyrinchus), sold in Niterói city, Rio de Janeiro state, Brazil.


This is a study of the organization of public health services in the state of Rio de Janeiro concerning the diagnosis of potentially malignant disorders. Secondary data from the database of the first phase of the Program for Enhancement for Access to and Quality of Primary Care were used. The implementation of actions at different levels for cancer prevention, the availability of diagnostic support services and the organization of the care network were assessed. The results show that only 58.8% of oral health teams record and monitor suspect cases; that only 47.1% reported having preferential channels for referring patients and there is great variation in waiting times to confirm the diagnosis. Local managerial and regional support actions can improve the organization of the care network for oral cancer prevention in the state. © 2016, Associacao Brasileira de Pos - Graduacao em Saude Coletiva. All rights reserved.


Patavino G.M.,University of Sao Paulo | Patavino G.M.,Blood System Research Institute | Patavino G.M.,Institute Matematica e Estatistica | De Almeida-Neto C.,University of Sao Paulo | And 29 more authors.
Transfusion | Year: 2012

BACKGROUND: Brazilian blood centers ask candidate blood donors about the number of sexual partners in the past 12 months. Candidates who report a number over the limit are deferred. We studied the implications of this practice on blood safety. STUDY DESIGN AND METHODS: We analyzed demographic characteristics, number of heterosexual partners, and disease marker rates among 689,868 donations from three Brazilian centers between July 2007 and December 2009. Donors were grouped based on maximum number of partners allowed in the past 12 months for each center. Chi-square and logistic regression analysis were conducted to examine associations between demographic characteristics, number of sex partners, and individual and overall positive markers rates for human immunodeficiency virus (HIV), human T-lymphotropic virus Types 1 and 2, hepatitis B virus, hepatitis C virus, and syphilis. RESULTS: First-time, younger, and more educated donors were associated with a higher number of recent sexual partners, as was male sex in São Paulo and Recife (p < 0.001). Serologic markers for HIV and syphilis and overall were associated with multiple partners in São Paulo and Recife (p < 0.001), but not in Belo Horizonte (p = 0.05, p = 0.94, and p = 0.75, respectively). In logistic regression analysis, number of recent sexual partners was associated with positive serologic markers (adjusted odds ratio [AOR], 1.2-1.5), especially HIV (AOR, 1.9-4.4). CONCLUSIONS: Number of recent heterosexual partners was associated with HIV positivity and overall rates of serologic markers of sexually transmitted infections. The association was not consistent across centers, making it difficult to define the best cutoff value. These findings suggest the use of recent heterosexual contacts as a potentially important deferral criterion to improve blood safety in Brazil. © 2011 American Association of Blood Banks.


PubMed | Federal University of Fluminense and Institute Matematica e Estatistica
Type: Journal Article | Journal: Ciencia & saude coletiva | Year: 2016

This is a study of the organization of public health services in the state of Rio de Janeiro concerning the diagnosis of potentially malignant disorders. Secondary data from the database of the first phase of the Program for Enhancement for Access to and Quality of Primary Care were used. The implementation of actions at different levels for cancer prevention, the availability of diagnostic support services and the organization of the care network were assessed. The results show that only 58.8% of oral health teams record and monitor suspect cases; that only 47.1% reported having preferential channels for referring patients and there is great variation in waiting times to confirm the diagnosis. Local managerial and regional support actions can improve the organization of the care network for oral cancer prevention in the state.


Campos T.F.,Institute Matematica e Estatistica | Wechsler S.,Institute Matematica e Estatistica
AIP Conference Proceedings | Year: 2012

ThisAIP_114891524 work aims to present a simple explanation about the ABC, Approximate Bayesian Computation or likelihood-free methods, a sampler algorithm class made to sampling from a posteriori distribution π(θ|x) in an independent way of the likelihood function. Along of this paper is to present a couple of ABCalgorithms such as the ABCRejection and the ABCMCMC. These algorithms are also applied to the beta model and to the normal-gamma model, in order to generate an univariate posterior distribution and a bivariate distribution, respectively. © 2012 American Institute of Physics.


Nascimento R.S.,Federal University of Fluminense | Fonseca A.B.M.,Institute Matematica e Estatistica | Franco R.M.,UFF | Miranda Z.B.,UFF
Revista Brasileira de Ciencia Avicola | Year: 2015

The aim of this study was to evaluate bacteriological characteristics and shelf life of three formulations of ostrich sausages (linguiças), only differing in lean meat percentage: Formula 1, 100% ostrich meat; Formula 2, 75% ostrich meat + 25% pork; and Formula 3, 50% ostrich meat + 25% pork + 25% chicken. All linguiças were vacuum-packed and stored at 5 ± 2ºC. Mesophilic and psychrotrophic bacteria, sulfitereducing Clostridia, coagulase-positive Staphylococci, and Escherichia coli were enumerated and Salmonella spp were isolated and identified. Initial mesophilic and psychotropic bacteria counts were high. During storage time, sulfite-reducing Clostridia, coagulase-positive Staphylococci, and Escherichia coli counts never reached the tolerance limit established by the Brazilian legislation. However, Salmonella was isolated from a Formula 2 sample on day 1, therefore, it was considered inappropriate for consumption. The shelf lives of Formulas 1 and 3 were below 12 and 8 days, respectively. If initial bacterial counts had been lower, the shelf life of the evaluated formulas would probably be longer. This study showed that ostrich meat trimmings can be successfully used in the production of ostrich linguiças, and that the formula containing ostrich meat as the only source of lean meat presented the longest shelf life. Ó 2015, Fundacao APINCO de Ciencia e Tecnologia Avicolas. All rights reserved.


De Carvalho R.R.,Federal University of Fluminense | Miranda Z.B.,Federal University of Fluminense | Fonseca A.B.M.,Institute Matematica e Estatistica | De Mesquita E.De.F.M.,Federal University of Fluminense
Revista Brasileira de Medicina Veterinaria | Year: 2013

This research was based on a survey among meat consumers in the period from 2008 to 2009. It was used 913 questionnaires, 656 in Niteroi and 257 in San Francisco of Itabapoana, districts of Rio de Janeiro state, Brazil, selected by their development indices particularly the IDHM. The questionnaires consisted of 16 questions with subdivisions in some of them where it was inquired about the consumer profile, their habits, choices and knowledge of the meat as food. Data were treated statistically revealing that the most consumed meat in the two cities is bovine meat, with a percentage of 36.6% and 52.5%, followed by poultry with 34.6% and 23%, by fish with 2.6% and 7,8% and swine with insignificant rates of 0.3% and 0%, respectively for Niteroi and San Francisco of Itabapoana. Other data was measured: knowledge of competence and action of the sanitary inspection agencies in the three spheres of government. The result was threatening due to the misinformation of these issues with negative indices as follows: for SIF 51% and 72.8%, for SIE 83.2% and 96.9%, and for SIM 92% and 99.2%, respectively, concerning Niteroi and San Francisco of Itabapoana districts. However, Municipal Health Surveillance is presented as an activity rather internalized by the interviewers on who takes care of public health issues in the district.


Rodrigues F.R.,Federal University of Fluminense | Lopes V.G.S.,Federal University of Fluminense | Lopez C.L.,Federal University of Fluminense | Filho P.J.S.,Federal University of Fluminense | And 3 more authors.
Jornal Brasileiro de Patologia e Medicina Laboratorial | Year: 2011

Introduction: The decline in the number of autopsies at hospitals and university hospitals has been widely reported, initially in developed countries and afterwards in several developing countries including Brazil. The causes for this trend are multiple and complex, encompassing religious, family and medical aspects. Among the latter, we highlight diagnostic reasons, which are characterized by major technological advances in clinical imaging associated with the underlying assumption that all diagnoses can currently be made before death. Furthermore, there is a growing concern about malpractice lawsuits. Objective: To demonstrate the sharp decrease in the number of autopsies at a university hospital in Brazil. Material and methods: We evaluated the number of autopsies conducted at the Pathology Department of Antonio Pedro University Hospital (HUAP), Niterói, Rio de Janeiro, and a database spreadsheet was created at Microsoft Excel. Results: From 1966 to 2009, 23,813 autopsies were performed, comprising 12,702 adults and 11,111 fetuses. The highest number of autopsies occurred from 1966 to 1998, when 23,321 autopsies were performed, comprising 12,482 adults and 10,839 fetuses. Whereas, there was a dramatic decline from 1999 to 2009, when 492 autopsies were performed, including 220 adults and 272 fetuses. Conclusion: We believe that the main cause of this reduction is the lack of information among laypeople and medical professionals, who have neglected the fact that knowledge is ultimately acquired through study, investigation and practice.

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