Institute Investigaciones Of Viandas Tropicales Inivit

Santo Domingo, Cuba

Institute Investigaciones Of Viandas Tropicales Inivit

Santo Domingo, Cuba
SEARCH FILTERS
Time filter
Source Type

Posada-Perez L.,University "Marta Abreu" of Las Villas | Montesinos Y.P.,University "Marta Abreu" of Las Villas | Guerra D.G.,Institute Investigaciones Of Viandas Tropicales Inivit | Daniels D.,University of Belize | Gomez-Kosky R.,Institute Investigaciones Of La Cana Of Azucar Inica
In Vitro Cellular and Developmental Biology - Plant | Year: 2017

A critical factor in somatic embryogenesis protocols in papaya (Carica papaya L.) has been incomplete germination of somatic embryos due to formation of a basal callus, which prevents the emission of the radicle. This work aims to achieve complete germination of somatic embryos in liquid and semi-solid culture media. The effect of the culture conditions on germination of somatic embryos using the RITA® temporary immersion system were evaluated as well as the effect of phloroglucinol on germination of somatic embryos in semi-solid culture medium. The results of using the RITA® culture medium with a combination of 0.02 μM BAP and 2.90 μM gibberellic acid had a good response for total germination (100%) but somatic embryos had only partial germination with 400 mg fresh mass. However, the optimum inoculum density was 200 mg fresh mass of somatic embryos which produced 100% total germination and 95% somatic embryos with complete germination. Also, it was possible to achieve complete germination of somatic embryos with low callus formation (13%) using phloroglucinol at a concentration of 475.8 μM on semi-solid culture medium. This is the first report of two biotechnological strategies for complete germination of plants from somatic embryos in the papaya cultivar `Maradol Roja´. © 2017 The Society for In Vitro Biology


Beovides Y.,Institute Investigaciones Of Viandas Tropicales Inivit | Fregene M.,Donald Danforth Plant Science Center | Gutierrez J.P.,Aereo | Milian M.D.,Institute Investigaciones Of Viandas Tropicales Inivit | And 10 more authors.
Biotechnology, Agronomy and Society and Environment | Year: 2015

A total of 36 microsatellites (SSR) markers were used to analyze the genetic diversity of 163 accessions of cultivated cassava (Manihot esculenta Crantz), 94 accessions of them from the Cuban Cassava Germplasm Collection and 69 genotypes from different countries and conserved at the International Center for Tropical Agriculture (Colombia). This study was carried out to determine genetic diversity within and between all accessions to promote their better use and conservation strategies. Thirty-four of those markers were used for the genetic diversity study based on their higher polymorphism. The Cuban cultivars showed the highest average allele number per loci with 5.8 and 100% of the loci were polymorphic, as well as those from Guatemala. The average proportion of individual heterozygocity observed (HO) was high (0.5918 ± 0.0351), while the highest HO rates were observed in groups of genotypes from Cuba (0.6016) and Tanzania (0.6459). The total heterozygocity (HT) was high (0.6538 ± 0.1770), but only 7.4% (GST = 0.0740 ± 0.0377) was due to differences between the five countries studied. Genetic differentiation coefficients (estimated by F-statistics) were low to moderate (FST > 0.04) and 17 unique alleles with low frequency were found in Cuban cultivars. The results provide the first molecular characterization of Cuban cassava genotypes and showed a wide diversity among landraces from Cuba. Application of this valuable information can be used for genetic diversity conservation and genotype identification studies for the genetic breeding program of cassava. © 2015, FAC UNIV SCIENCES AGRONOMIQUES GEMBLOUX .All Rights Reserved.

Loading Institute Investigaciones Of Viandas Tropicales Inivit collaborators
Loading Institute Investigaciones Of Viandas Tropicales Inivit collaborators