Institute Investigaciones Of Sanidad Vegetal

Havana, Cuba

Institute Investigaciones Of Sanidad Vegetal

Havana, Cuba
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Javer-Higginson E.,Institute Investigaciones Of Sanidad Vegetal | Acina-Mambole I.,CIRAD | Gonzalez J.E.,Institute Investigaciones Of Viandas Tropicales | Font C.,Institute Investigaciones Of Sanidad Vegetal | And 4 more authors.
European Journal of Plant Pathology | Year: 2014

Banana streak viruses (BSV) belong to the genus Badnavirus of the family Caulimoviridae. They cause banana streak disease on banana and plantains worldwide. The recent detection of BSV in Cuba has prompted a nationwide research effort focused on the occurrence, prevalence and diversity of BSV species on dessert type banana on the island. Indexing by multiplex immunocapture-PCR (M-IC-PCR) performed on samples collected throughout the country showed that the overall prevalence of Banana streak OL virus, Banana streak GF virus and Banana streak IM virus is low in Musa acuminata genotypes in Cuba. However, the prevalence of BSV species Mysore (BSMYV) was surprisingly high in samples of cv Yangambi km5 collected from distinct and distant locations. The presence in Cuba of an as yet unreported BSV species was also investigated, showing that Banana steak VN virus is also present in Musa acuminata genotypes. © 2013 KNPV.

Gonzalez Rizo A.,Institute Medicina Tropical Pedro Kouri Ipk | Menendez Diaz Z.,Institute Medicina Tropical Pedro Kouri Ipk | Garcia Garcia I.,Institute Medicina Tropical Pedro Kouri Ipk | Anaya Martinez J.,Institute Medicina Tropical Pedro Kouri Ipk | And 5 more authors.
Revista Cubana de Medicina Tropical | Year: 2016

Introduction: Bacillus thuringiensis is a sporulated gram-positive bacterium, well known mainly for its pathogenic action against insects. Some strains synthesize thermostable nucleoside analog secondary metabolites identified as beta-exotoxins. The World Health Organization has recommended that beta-exotoxin-producing Bacillus thuringiensis strains not be used for insect control, due to their unspecific range of action and their toxicity to mammalian cells. Objective: Detect by means of bioassays the presence of beta-exotoxins in Cuban isolates of Bacillus thuringiensis with entomopathogenic activity against Aedes aegypti (Linneaus, 1762). Methods: Aaedes aegypti eggs were put in 500 mL water at 37 °C until eclosion. Next, 66 mg fish flour were added to 10 mL of the supernatant of the autoclaved culture. Living individuals were counted daily for 7 days. Statistical processing of mortality data was conducted with one-way analysis of variance (ANOVA) and Tukey's multiple comparison. The differences found were considered to be statistically significant for p< 0.05. The presence of beta-exotoxins was directly confirmed by spectrophotometry. Results: The presence of beta-exotoxins was detected by the two methods applied in only one isolate (A51). Conclusions: Absence of beta-exotoxins in 11 isolates with marked entomopathogenic activity against Aedes aegypti confirms their potential for the development of new biolarvicides. © 2016, Editorial Ciencias Medicas. All rights reserved.

Milishnikov A.N.,RAS A.N. Severtsov Institute of Ecology and Evolution | Borotto R.,Institute Investigaciones Of Sanidad Vegetal | Orlov V.N.,RAS A.N. Severtsov Institute of Ecology and Evolution
Russian Journal of Genetics | Year: 2010

The work attempts to explain the previously discovered phenomenon of excessive taxonomic status of the species and subspecies of Cuban hutias relative to their protein genetic divergence. The reasons underlying the fragmentary distribution of the species belonging to the family Capromyidae over the Antilles and Bahamas is also considered. Samples of four geographically distant hutia (genera Capromys and Mysateles) populations inhabiting different biotypes were assessed according to 32 allozyme loci. It has been demonstrated that the interpopulation and subspecies differentiation in the gene frequencies in these genera is very low. As for the pattern of polymorphism (Shannon's measure), the populations and subspecies within these genera are well differentiated and adequate to their morphological differentiation. This suggests that the genetic divergence pattern of hutias in post-Pleistoce ne was predominantly determined by selection on the background of a relatively rapid formation of multiple morphotypes. It is assumed that the evolution within the family Capromyidae in the earlier and the later periods was associated with the geomorphological and climatic events in the history of the Earth. This standpoint provides for explaining the phenomena of both the excessive taxonomic status of the Cuban Capromyinae and the fragmentary distribution of these species over the Antilles and Bahamas, amazing at a first glance. © Pleiades Publishing, Inc., 2010.

Furrazola E.,Institute Ecologia y Sistematica | Covacevich F.,CONICET | Torres-Arias Y.,Institute Ecologia y Sistematica | Rodriguez-Rodriguez R.M.,Institute Ecologia y Sistematica | And 4 more authors.
Revista de Biologia Tropical | Year: 2015

Despite the ubiquity and importance of indigenous arbuscular mycorrhizal fungi (AMF) for plant ecosystems; functioning of indigenus mycorrhizal symbiosis (IMS) and related environmental factors at coastal Caribbean ecosystems remains still scarce. In order to determine functionality of IMS under contrasting land uses and wet seasons from Cuba, the influence of the water stress on some AMF functionality parameters from a semi-natural savannah (NS), a recovered savannah (RS) and an agro-ecosystem (AG) from the Managed Floristic Reserve San Ubaldo-Sabanalamar, Pinar del Rio, Cuba were assessed during two-years. Soil and root samples were collected in April and October, during the dry and wet seasons, respectively, in 2008 and 2010. Four plots in each ecosystem were selected, and five soil sub-samples were randomly collected, bulked, mixed homogeneously and used as the composite sample per plot. The host plant root biomass, arbuscular mycorrhizal colonization of the host plant, density of the intraradical and extraradical AMF mycelia, fungal endophyte biomass and AMF spore density were assessed. The host plant root biomass increased in the NS environment during the dry season, and approximately 12.85g root/dm3 dry soil was recorded. The colonization degree were significantly higher in all environments during the wet season of the second year, with means ranging from 79% to 89%. The extraradical mycelia were significantly more abundant in the dry season of the second year in all environments, with a maximum of 279mg/dm3 in the RS ecosystem. The density of AMF spores was highest in the dry season of the second year for the three studied ecosystems. The RS ecosystem hosted 5 670 spores/100g dry soil. In general, the influence of rainfall seasonality on the function of AMF was stronger than the influence of ecosystem management. The root biomass and extraradical mycelia were high in the dry seasons, suggesting strategies to increase the volume of soil for the mutual benefit of the symbionts. The increase in spore density during the dry seasons appears as an adaptation allowing AMF to survive period of water shortage. This study improves our understanding of the adaptative responses of arbuscular mycorrhizal symbiosis to seasonal variations in soil water availability. © 2015, Universidad de Costa Rica. All Rights Reserved.

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