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Rojas M.M.,University of Habana | Hernandez A.,University of Habana | Rives N.,Institute Investigaciones Of Granos | Tejera B.,University of Habana | And 2 more authors.
Acta Biologica Colombiana | Year: 2012

Rabbit polyclonal antisera against indoleacetic acid (IAA) bound to nitrocellulose membrane were obtained, which exhibited a high titer and specificity. The dot immunobinding technique with colloidal gold was used to detect auxin production by several strains belonging to Gluconacetobacter, Herbaspirillum, Azospirillum, Pseudomonas, Burkholderia and Bacillus genera, using culture supernatants as antigens. Moreover, auxins production was quantified by the Salkowski's method to corroborate the previous results. It was found that that all microorganisms studied produce IAA and the feasibility of using these antisera to detect the metabolite was confirmed. Taking into account the potentialities of plant growth promoting bacteria as biofertilizers, the use of these antisera for a rapid and easy detection of IAA in bacteria associated with important crops is thus recommended.

Muniz Ugarte O.,Institute Suelos | Cabello Martinez R.,Institute Investigaciones Of Granos | Socorro Quesada M.,Institute Investigaciones Of Granos | Nuviola Montoya A.,Institute Suelos | Duenas Vega G.,Institute Suelos
Spanish Journal of Soil Science | Year: 2012

This study evaluated the effect of using the green manure Sesbania rostrata as a preceding crop on the agricultural yield of rice. The rice was cultivated with two levels of mineral fertilizers (50 and 100% of the economical optimum level of N-P-K). Additionally, the contribution to the nitrogen nutrition of rice from the green manure previously incorporated into the soil was studied in pots using 15N stable isotope analysis. The results indicated that the Sesbania rostrata incorporation allowed the paddy rice yield to increase by at least 1 Mg ha-1 and to save 50% of the mineral fertilization employed, during two consecutive harvests. The 15N isotope analysis showed that although the N accumulation by the crop was significantly stimulated, the green manure N did not contribute directly to the growth of the rice plants, at least during their first cycle. Nevertheless, it is expected that in the medium term, the organic N derived from this green manure would restore the soil mineralized N extracted by the rice crop, thereby balancing the N losses in the system and even improving the soil nitrogen fertility. © 2012, Universia. All rights reserved.

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