Institute Investigaciones en Fruticultura Tropical IIFT

Playa, Cuba

Institute Investigaciones en Fruticultura Tropical IIFT

Playa, Cuba

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Lepitre V.,CIRAD - Agricultural Research for Development | Nansot G.,CIRAD - Agricultural Research for Development | Grangeon R.,CIRAD - Agricultural Research for Development | Pomies V.,CIRAD - Agricultural Research for Development | And 11 more authors.
Acta Horticulturae | Year: 2010

An integrated parental linkage map of guava (Psidium guajava L., 2n = 22) based on AFLP and SSR markers has been constructed for the mapping population MP1 derived from a cross between two heterozygous guava cultivars ("Enana" x "N6"). A total of 1103 segregating AFLP markers obtained from 119 AFLP primer combinations were produced for linkage mapping. In addition, 171 SSR maker loci were analysed which generated 258 allelic fragments. In total 1364 AFLP and SSR markers were available for linkage mapping. The integrated linkage map of MP1 contains 578 markers (452 AFLPs, 126 SSRs) distributed on 11 linkage groups corresponding to the 11 chromosomes of the haploid guava genome. This map has a length of 2179 cM and an average linkage group length of 198 cM. In addition, a total of 126 so-called RF0 markers and 146 so-called associated markers were determined. The current MP1 map will be completed by the integration of the individual maps derived from other available guava mapping populations. This represents a decisive step towards a saturated genetic reference map of P. guajava in support of accelerated breeding in guava by marker-assisted selection.


Valdes-Infante J.,Institute Investigaciones en Fruticultura Tropical IIFT | Rodriguez-Medina N.N.,Institute Investigaciones en Fruticultura Tropical IIFT | Velasquez B.,Institute Investigaciones en Fruticultura Tropical IIFT | Rivero D.,Institute Investigaciones en Fruticultura Tropical IIFT | And 6 more authors.
Acta Horticulturae | Year: 2010

Agro-morphologic traits and molecular markers were compared in terms of their polymorphism level, discriminating power, and informativeness for 23 genotypes assembled in the Cuban guava germplasm collection. AFLP and SSR markers were powerful techniques for guava discrimination and variety identification, but the high level of polymorphic loci detected by the dominant AFLP marker highlights the discriminating capacity of this genetic marker. With a single AFLP primer combination all of the individuals were identified, while only a few genotypes can be differentiated with a single SSR primer combination or by morphological variables. The higher values of expected heterozygosity were detected by SSR. This value doubled the one obtained with AFLP, and reflects the high level of informativeness of this marker, due to the multiallelic and co-dominant nature of SSR; which makes them suitable for diversity studies. The morphologic diversity index provided a good estimate of diversity among guava accessions when phenotypic traits of high heritability were used, and it was comparable with the expected heterozygosity scored with DNA markers. The value of this index was the lowest. The assay efficiency index (Ai) and marker index (MI) had the same pattern of variation than discrimination capacity (D), number of banding pattern (I), number of unique banding pattern (Iu) and number of effective pattern (P) for both molecular markers. Then, these indexes are probably indicators of the discriminating capacity in guava.


Valdes-Infante J.,Institute Investigaciones en Fruticultura Tropical IIFT | Rodriguez N.N.,Institute Investigaciones en Fruticultura Tropical IIFT | Velasquez B.,Institute Investigaciones en Fruticultura Tropical IIFT | Rivero D.,Institute Investigaciones en Fruticultura Tropical IIFT | And 6 more authors.
Acta Horticulturae | Year: 2010

Guava (Psidium guajava L.) is one of the economically most important fruit trees of the Myrtaceae. Until nowadays, guava breeding programs in Cuba have been limited to the selection and introduction of genotypes with important agronomic characteristics, but focussed studies on genetic diversity which is very important for the identification of potential parents for breeding programs, had not been undertaken. The utilization of microsatellite (SSR) markers for guava germplasm characterization and gene bank management was the main objective of this work. A total of 34 different SSR alleles ranging from three to seven per locus were detected in the examined genotypes with an average number of putative alleles per locus of 4.57. Out of these, twenty-four alleles were classified as common alleles, out of which 10 were widespread and 14 sporadic. Ten alleles were classified as rare, from which 7 were sporadic and 3 localised. For all SSR loci, a major number of homozygote genotypes were identified, except for the SSR locus mPgCIR09. This result reflected the medium to low levels of heterozygosis detected, which ranged from 0.08 to 0.54 with 0.38 as the total average for this parameter. Genotypes showing cultivar-specific markers were 'Darío 19-2'; 'Belic L-98'; 'BG 76-23'; 'Belic L-205' and 'Microguayaba'. These could be important materials for conservation purposes. The high number of common alleles detected suggested that most of the plant material analyzed shares a common genetic ancestor. The microsatellites evaluated could play an important role in the identification of guava accessions representing an interesting gene pool for ex situ maintenance.


Rodriguez-Medina N.N.,Institute Investigaciones en Fruticultura Tropical IIFT | Valdes-Infante J.,Institute Investigaciones en Fruticultura Tropical IIFT | Velasquez B.,Institute Investigaciones en Fruticultura Tropical IIFT | Rivero D.,Institute Investigaciones en Fruticultura Tropical IIFT | And 6 more authors.
Acta Horticulturae | Year: 2010

Guava (Psidium guajava L.) belongs to the Myrtaceae family and is native to the tropical and subtropical regions of America. It produces a fruit of high nutritional value due to its mineral and vitamin content. During the past 2 decades, classical methods to evaluate genetic variation have been complemented by molecular DNA techniques. There is a great potential for the application of these molecular markers to fruit crops. The objective of this research was to compare results derived from individual versus combined data sets for varietal identification and diversity estimation in guava germplasm by different DNA markers. AFLP data permitted the discrimination of all the accessions evaluated, but diversity groups were not observed. Microsatellite (SSR) markers detected seven diversity groups, although all the accessions could not be differentiated. The combined AFLP and SSR data offered similar results only when AFLP markers were used. Although coincidences were identified in individuals and combined dendrograms, the integration of the information derived by each marker system individually permitted a more accurate estimation of guava germplasm diversity. This corroborates the strengths and constraints of each data set. Thus, the congruence or correspondence among the results derived from individual data sets is the most important point to consider before combining different types of data.


Banguela-Castillo A.,Institute Investigaciones en Fruticultura Tropical IIFT | Ramos-Gonzalez P.L.,Institute Investigaciones en Fruticultura Tropical IIFT | Pena-Marey M.,Institute Investigaciones en Fruticultura Tropical IIFT | Tanaka F.A.O.,University of Sao Paulo | And 3 more authors.
Crop Protection | Year: 2015

Muscodor is an endophytic fungal genus whose members produce volatile organic compounds (VOCs) with broad antimicrobial, nematocidal and insecticidal activities. This study describes the isolation and characterization of a new strain of Muscodor albus, designated as M. albus aa3, from wild pineapple (Ananas ananassoides) plants collected in Havana, Cuba. In vitro cultures of M. albus aa3 on both potato dextrose agar media (PDA) and paddy produced VOCs with antimicrobial activity against Escherichia coli and plant pathogens, including several species of the Phytophthora genus and the wood rot fungus Fomitiporia maxonii; but innocuous to the beneficial mycopathogen Trichoderma koningii. GC/MS analysis indicated the unique composition of the mixture of VOCs emitted by aa3, in which sesquiterpenes represent the most abundant compounds. VOCs emitted during the growth of M. albus aa3 on paddy grains protected Persian lime (Citrus×. latifolia Tanaka) fruits from infection by Phytophthora nicotianae Breda de Haan, suggesting the potential use of this fungus for postharvest biofumigation. Isolation of M. albus from wild pineapple points out this plant as a susceptible host to be colonized by distinct species of the Muscodor genus. © 2015.


Banguela A.,Institute Investigaciones en Fruticultura Tropical IIFT | Rodriguez R.,Institute Investigaciones en Fruticultura Tropical IIFT
Journal of Biotechnology | Year: 2011

Bacterial levansucrase (EC 2.4.1.10) converts sucrose into non-linear levan consisting of long β(2,6)-linked fructosyl chains with β(2,1) branches. Bacterial levan has wide food and non-food applications, but its production in industrial reactors is costly and low yielding. Here, we report the constitutive expression of Gluconacetobacter diazotrophicus levansucrase (LsdA) fused to the vacuolar targeting pre-pro-peptide of onion sucrose:sucrose 1-fructosyltransferase (1-SST) in tobacco, a crop that does not naturally produce fructans. In the transgenic plants, levan with degree of polymerization above 104 fructosyl units was detected in leaves, stem, root, and flowers, but not in seeds. High levan accumulation in leaves led to gradual phenotypic alterations that increased with plant age through the flowering stage. In the transgenic lines, the fructan content in mature leaves varied from 10 to 70% of total dry weight. No oligofructans were stored in the plant organs, although the in vitro reaction of transgenic LsdA with sucrose yielded β(2,1)-linked FOS and levan. Transgenic lines with levan representing up to 30mgg-1 of fresh leaf weight produced viable seeds and the polymer accumulation remained stable in the tested T1 and T2 progenies. The lsdA-expressing tobacco represents an alternative source of highly polymerized levan. © 2011 Elsevier B.V.


Gonzalez-Perez L.,University of Habana | Becker D.,Max Planck Institute for Plant Breeding Research | Rodriguez-Medina N.N.,Institute Investigaciones en Fruticultura Tropical IIFT | Valdes-Infante J.,Institute Investigaciones en Fruticultura Tropical IIFT | And 2 more authors.
Acta Horticulturae | Year: 2010

Guava (Psidium guajava L.) is native to the tropical regions of the Americas. Its economic importance is related to the use of its fresh fruit that contains high amounts of vitamins A and C, dietary fiber and calcium and to processed food products (juice, cream, marmalade, desserts etc.). Thus, in addition to the demand for local consumption, guava presents a valuable opportunity for export. The molecular analysis of the guava genome is part of an international project ("GUAVAMAP") funded by the European Commission under INCO-DEV-2. The objective of the present work was to identify gene families representing putative genes for resistance (resistance gene-like (RGL) sequences) and homeotic genes involved in plant development. For this, a guava genomic cosmid (COS) library was established for the economically important Cuban cultivar "Enana roja cubana". COS clones (6-7 equivalents of the guava genome) were individualized and spotted in duplicates onto membrane filters (macroarrays). Molecular hybridization was done with heterologous probes for resistance (RGL sequences) and homeotic genes (MADX-box and HOMEO-box genes). The macroarray hybridization data were verified by Southern blot analysis on isolated COS clone DNAs. A total of 117 positive clones for RGL sequences, 37 for MADS-box and 22 for HOMEO-box were identified. Guava mutants with respect to growth habit and flower morphology are present in the Cuban germplasm collection and may provide the material for a possible association of the mutant phenotype to the isolated genes.


Rodriguez-Medina N.N.,Institute Investigaciones en Fruticultura Tropical IIFT | Fermin G.A.,University of Los Andes, Venezuela | Valdes-Infante J.,Institute Investigaciones en Fruticultura Tropical IIFT | Velasquez B.,Institute Investigaciones en Fruticultura Tropical IIFT | And 4 more authors.
Acta Horticulturae | Year: 2010

Usually, the easiest assessment of genetic variation is through morphological or phenotypic measures. Also, the problems arising from the identification of a great number of plants in a fruit tree collection as well as those concerned with the protection of varietal names ask for the use of specific descriptors. In consequence, the guidelines published in 1987 by the International Union for the Protection of New Varieties of Plants (UPOV) provided the strategy for the conduction of tests for distinctness, homogeneity, and stability in guava. These descriptors are an important means for the standardization of guava characterization worldwide and provide an easy and rapid way to discriminate between guava phenotypes. The descriptors generally include highly heritable traits that can be easily detected visually. In addition, they may include a limited number of additional characters desirable by user consensus. The current study offers more than 50 descriptors, many of them accompanied by illustrations to help the selection of different variable states. The number of variables recommended for grouping accessions and the evaluated characters was increased in relation to the ones listed in the UPOV guidelines. Classes of quantitative traits were established to permit the evaluation of these characters for both quantitative and qualitative data. Values from 1 to 9 were used to design different states of each descriptor, thus allowing the establishment of an electronic database and data processing. A minimum of highly discriminative descriptors was identified.


Rodriguez-Medina N.N.,Institute Investigaciones en Fruticultura Tropical IIFT | Valdes-Infante J.,Institute Investigaciones en Fruticultura Tropical IIFT | Gonzalez G.,Institute Investigaciones en Fruticultura Tropical IIFT | Fuentes V.,Institute Investigaciones en Fruticultura Tropical IIFT | Canizares J.,Institute Investigaciones en Fruticultura Tropical IIFT
Acta Horticulturae | Year: 2010

In the first half part of the last century, guava (Psidium guajava) was not cultured in Cuban, and breeding programs were not formally developed. In 1965, with the foundation of the Germplasm Bank of Tropical and Subtropical Fruit Trees (Havana Province), the principal Cuban guava collection was established. This gene bank was formed from three different sources: (i) foreign cultivars, (ii) plants prospected in different localities throughout the country, and (iii) selected genotypes segregating from open-pollinated seeds. During this period, an incipient breeding program was started, which resulted in the identification of nine cultivars with 'E.E.A. 1-23', 'E.E.A. 18-40', 'Belic L-207', 'Belic L-215', 'Belic L-97' and 'E.E.A. 28-44' showed the highest yields. The two formers are also dwarf genotypes and nowadays constitute the main cultivars for commercial production in Cuba. In 2001, a breeding program by controlled crosses produced a total of 354 hybrid plants from which 25 dwarf genotypes were finally selected on the basis of quantitative and qualitative traits for the establishment of new commercial cultivars. The three populations from these crosses were further employed to develop guava genetic linkage maps and to map QTLs using morph-agronomic characters and molecular markers. The combination of molecular marker methods and phenotypic description was greatly facilitated cultivar identification, diversity estimation, and elite genotypes recommendation in guava germplasm. Other modern biotechnology techniques such as in vitro culture and the detection of candidate genes for resistance and plant development can be potential tools to assist guava breeding program.

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