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Ruiz-Gomez F.J.,University of Cordoba, Spain | Sanchez-Cuesta R.,University of Cordoba, Spain | Navarro-Cerrillo R.M.,University of Cordoba, Spain | Perez-de-Luque A.,Institute Investigacion Y Formacion Agraria Y Pesquera Of Andalucia Ifapa
Plant Methods | Year: 2012

Phytophthora cinnamomi Rands. is an important root rot pathogen widely distributed in the north hemisphere, with a large host range. Among others diseases, it is known to be a principal factor in the decline of holm oak and cork oak, the most important tree species in the dehesa ecosystem of south-western Spain. Previously, the focus of studies on P. cinnamomi and holm oak have been on molecular tools for identification, functional responses of the host, together with other physiological and morphological host variables. However, a microscopic index to describe the degree of infection and colonization in the plant tissues has not yet been developed. A colonization or infection index would be a useful tool for studies that examine differences between individuals subjected to different treatments or to individuals belonging to different breeding accessions, together with their specific responses to the pathogen. This work presents a methodology based on the capture and digital treatment of microscopic images, using simple and accessible software, together with a range of variables that quantify the infection and colonization process. © 2012 Ruiz-Gómez et al.; licensee BioMed Central Ltd. Source


Hermida M.,University of Santiago de Compostela | Rodriguez-Ramilo S.T.,University of Vigo | Rodriguez-Ramilo S.T.,Instituto Nacional Of Investigacion Y Tecnologia Agraria Y Alimentaria | Hachero-Cruzado I.,Institute Investigacion Y Formacion Agraria Y Pesquera Of Andalucia Ifapa | And 5 more authors.
Aquaculture | Year: 2014

Genetic maps constitute valuable tools to detect genomic regions associated with complex traits and to go forward to understand their genetic basis. Flatfish include several species of great commercial value for which increasing genomic resources are available including genetic maps and EST databases. Application of comparative mapping strategies to flatfish is relevant to obtain genetic information associated with productive traits. The brill (Scophthalmus rhombus) is a flatfish species closely related to turbot (S. maximus) whose meat is highly appreciated in the Spanish market. The Junta de Andalucía local Government has begun a program to adapt this species to captivity for its future production. In this study, we developed the first genetic map of brill using the current turbot genetic map as starting point. This strategy enabled us to select a number of homogeneously distributed markers in the turbot map and to apply cross-species microsatellite amplification to obtain informative markers. Nearly two hundred microsatellites from the framework turbot map were used for validation, and 100 markers were finally informative for mapping. The parents and offspring of the two families (54 and 88, respectively) used to construct the genetic map were genotyped with this panel. All markers, except eleven, were successfully grouped and ordered in 24 linkage groups. Linkage groups and order of markers were highly consistent with the previous turbot genetic map. Linkage map information was used to carry out a preliminary study on growth-related QTL for body weight, length and Fulton's condition factor in the two families, as the main phenotypic traits of interest in this species. © 2013 Elsevier B.V. Source


Hachero-Cruzado I.,Institute Investigacion Y Formacion Agraria Y Pesquera Of Andalucia Ifapa | Fornies A.,Institute Ciencias Marinas Of Andalucia Icman | Herrera M.,Institute Investigacion Y Formacion Agraria Y Pesquera Of Andalucia Ifapa | Mancera J.M.,University of Cadiz | Martinez-Rodriguez G.,Institute Ciencias Marinas Of Andalucia Icman
Fish Physiology and Biochemistry | Year: 2013

The aims of the present study were to characterize sperm quality and to quantify seasonal changes in sexual hormone (testosterone [T], 11-ketotestosterone [11-KT] and 17,20β-dihydroxypregn-4-en-3-one [17,20β-P]) levels in male brill (Scophthalmus rhombus) plasma, as well as to test a more intensive sampling strategy to establish relationships between sex steroid levels and sperm production parameters. Sperm concentration ranged from 0. 5 to 3. 1 × 109 spermatozoa mL-1, and changes in sperm quality parameters depending on sampling date were observed. Plasma sexual steroid levels remained high and changed in parallel during the spawning season and afterwards decreased to very low levels in summer. The analysis of annual changes of 11-KT and T ratios suggests that 11-KT can be the main circulating androgen for stimulating spermatogenesis in S. rhombus and that T could be involved in the beginning of spermatogenesis through the positive feedback on brain-pituitary-gonad axis. Finally, daily 11-KT and T levels showed similar patterns of variation in males sampled, whereas 17,20β-P amounts showed somewhat opposite trends. These differences could be related with the different role of androgens and progestin during the spermatogenesis. © 2012 Springer Science+Business Media B.V. Source


Cruzado I.H.,Institute Investigacion Y Formacion Agraria Y Pesquera Of Andalucia Ifapa | Cruzado I.H.,Centro El Toruno | Rodriguez E.,University of La Laguna | Herrera M.,Institute Investigacion Y Formacion Agraria Y Pesquera Of Andalucia Ifapa | And 2 more authors.
Aquaculture Research | Year: 2013

Brill (Scophthalmus rhombus L.) is a flatfish considered of special interest for aquaculture diversification, but the high mortality observed during the early larval rearing is the main obstacle to commercial culture. The objective of this study was to contribute to the knowledge of nutrient utilization of early-hatched larvae, characterizing the changes in lipid and protein contents during embryogenesis and the yolk-sac larval stage of S. rhombus. Total lipid, lipid classes and fatty acid contents remained constant during embryogenesis and yolk-sac larval development, except for phosphatidylethanolamine and phosphatidylinositol, which increased in quantity during the yolk-sac larval stage. On the other hand, total protein (including non-protein nitrogen) and amino acids decreased their contents in both periods, especially at hatching. The decrease only in the serine, glutamic acid, proline and lysine contents during embryogenesis suggests a selective use of amino acids during this phase. Unlike embryogenesis, amino acids loss during hatching appears to be non-selective, and almost all amino acids (essential and non-essential) decreased. Our results suggest that there is higher catabolism of protein vs. lipid during embryogenesis and the yolk-sac larval stage of S. rhombus. © 2012 John Wiley & Sons Ltd. Source


Cruzado I.H.,Institute Investigacion Y Formacion Agraria Y Pesquera Of Andalucia Ifapa | Herrera M.,Institute Investigacion Y Formacion Agraria Y Pesquera Of Andalucia Ifapa | Quintana D.,Institute Investigacion Y Formacion Agraria Y Pesquera Of Andalucia Ifapa | Rodiles A.,Institute Investigacion Y Formacion Agraria Y Pesquera Of Andalucia Ifapa | And 3 more authors.
Aquaculture Research | Year: 2011

The present study examines the total lipid (TL) and fatty acid (FA) composition in eggs of brill Scophthalmus rhombus L. and the possible relationships with their quality parameters. Wild broodstocks were caught and maintained in captivity until eggs were collected. A lipid characterization of each egg batch was conducted in TL, lipid classes (LC) and FA of TL. The TL content was lower than the values reported for other flatfish species, showing high levels of sterol esters (SE). High viability rates were related to higher lipid reserves. Higher cholesterol (CHO) was linked to higher egg viability, whereas SE could have an opposite effect. Comparison of female quality showed that lipid composition was more related to egg batches than to individual females. However, multivariate analysis did not show a clear correlation between lipid composition and brill egg quality, neither as individual components nor as a whole profile of LC or FA. Our results suggest that some lipid components (phosphatidylcholine, CHO, SE, monounsaturated, eicosapentaenoic acid) could be related to differences in spawning quality, although these were not the only factors involved in these differences. Thus, these lipid components could be considered to be descriptors of the differences found in the rates of brill quality. © 2010 Blackwell Publishing Ltd. Source

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