Institute Investigacion Y Formacion Agraria Y Pesquera Of Andalucia Ifapa

Pino del Río, Spain

Institute Investigacion Y Formacion Agraria Y Pesquera Of Andalucia Ifapa

Pino del Río, Spain
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Afforestation programs in Spain were performed mainly between 1930-1970 decades. The study of the state and functioning of these anthropogenic forests has a wide debate among scientists and stakeholders. Forest inventories represent a crucial source of data to evaluate the long-term functioning of these forests at broad spatial scales. This article shows several tools to identify and select plots from the third Spanish Forest Inventory composed by uniform afforestations. The functions intend to facilitate the work of scientists and technicians whose objective could be influenced by the presence or absence of these artificial forest type. Those functions are a first attempt to include or exclude plots that are composed by artificially afforested forests. I also present some preliminary results, and discuss strengths, weaknesses and future directions relative to the use of these functions. © 2016 Los Autores. Editado por la AEET.


Coste O.,Institute Investigacion Y Formacion Agraria Y Pesquera Of Andalucia Ifapa | Malta E.-J.,IFAPA Centro Agua del Pino | Lopez J.C.,Institute Investigacion Y Formacion Agraria Y Pesquera Of Andalucia Ifapa | Fernandez-Diaz C.,Institute Investigacion Y Formacion Agraria Y Pesquera Of Andalucia Ifapa
Algal Research | Year: 2015

Green macroalgae of the genus Ulva have complex and hardly degradable polysaccharidic extracellular matrix. One of its main components, the ulvan, is a 3-sulfated rhamnoglucuronan that has a wide range of properties and is a source of rare sugars. The production of mono- and oligo-saccharides from this polysaccharide could motivate its use to an industrial scale. Enzymatic tools to realize this process are still scarce. Here we describe the activity of an enzyme crude extract proceeding from a new Alteromonas species isolated from the gut of Gammarus insensibilis, an amphipod from southern Spanish salt marshes. HPTLC separation and NMR spectrometry allowed describing the formation of low molecular weight oligosaccharides formed principally of unsaturated 3-sulfated rhamnose and uronic acids. The control of hydrolysis kinetics allowed preparing different molecular weight fractions down to a 5. kDa oligosaccharides mix. These results highlight the presence of a potentially new β-lyase produced by these hitherto undescribed bacteria. This work identifies a new source of enzymes with potential application for the production of high added value compounds from Ulva sp. © 2015 Elsevier B.V..


Cruzado I.H.,Institute Investigacion Y Formacion Agraria Y Pesquera Of Andalucia Ifapa | Herrera M.,Institute Investigacion Y Formacion Agraria Y Pesquera Of Andalucia Ifapa | Quintana D.,Institute Investigacion Y Formacion Agraria Y Pesquera Of Andalucia Ifapa | Rodiles A.,Institute Investigacion Y Formacion Agraria Y Pesquera Of Andalucia Ifapa | And 3 more authors.
Aquaculture Research | Year: 2011

The present study examines the total lipid (TL) and fatty acid (FA) composition in eggs of brill Scophthalmus rhombus L. and the possible relationships with their quality parameters. Wild broodstocks were caught and maintained in captivity until eggs were collected. A lipid characterization of each egg batch was conducted in TL, lipid classes (LC) and FA of TL. The TL content was lower than the values reported for other flatfish species, showing high levels of sterol esters (SE). High viability rates were related to higher lipid reserves. Higher cholesterol (CHO) was linked to higher egg viability, whereas SE could have an opposite effect. Comparison of female quality showed that lipid composition was more related to egg batches than to individual females. However, multivariate analysis did not show a clear correlation between lipid composition and brill egg quality, neither as individual components nor as a whole profile of LC or FA. Our results suggest that some lipid components (phosphatidylcholine, CHO, SE, monounsaturated, eicosapentaenoic acid) could be related to differences in spawning quality, although these were not the only factors involved in these differences. Thus, these lipid components could be considered to be descriptors of the differences found in the rates of brill quality. © 2010 Blackwell Publishing Ltd.


Cruzado I.H.,Institute Investigacion Y Formacion Agraria Y Pesquera Of Andalucia Ifapa | Cruzado I.H.,Centro El Toruno | Rodriguez E.,University of La Laguna | Herrera M.,Institute Investigacion Y Formacion Agraria Y Pesquera Of Andalucia Ifapa | And 2 more authors.
Aquaculture Research | Year: 2013

Brill (Scophthalmus rhombus L.) is a flatfish considered of special interest for aquaculture diversification, but the high mortality observed during the early larval rearing is the main obstacle to commercial culture. The objective of this study was to contribute to the knowledge of nutrient utilization of early-hatched larvae, characterizing the changes in lipid and protein contents during embryogenesis and the yolk-sac larval stage of S. rhombus. Total lipid, lipid classes and fatty acid contents remained constant during embryogenesis and yolk-sac larval development, except for phosphatidylethanolamine and phosphatidylinositol, which increased in quantity during the yolk-sac larval stage. On the other hand, total protein (including non-protein nitrogen) and amino acids decreased their contents in both periods, especially at hatching. The decrease only in the serine, glutamic acid, proline and lysine contents during embryogenesis suggests a selective use of amino acids during this phase. Unlike embryogenesis, amino acids loss during hatching appears to be non-selective, and almost all amino acids (essential and non-essential) decreased. Our results suggest that there is higher catabolism of protein vs. lipid during embryogenesis and the yolk-sac larval stage of S. rhombus. © 2012 John Wiley & Sons Ltd.


Ruiz-Gomez F.J.,University of Cordoba, Spain | Sanchez-Cuesta R.,University of Cordoba, Spain | Navarro-Cerrillo R.M.,University of Cordoba, Spain | Perez-de-Luque A.,Institute Investigacion Y Formacion Agraria Y Pesquera Of Andalucia Ifapa
Plant Methods | Year: 2012

Phytophthora cinnamomi Rands. is an important root rot pathogen widely distributed in the north hemisphere, with a large host range. Among others diseases, it is known to be a principal factor in the decline of holm oak and cork oak, the most important tree species in the dehesa ecosystem of south-western Spain. Previously, the focus of studies on P. cinnamomi and holm oak have been on molecular tools for identification, functional responses of the host, together with other physiological and morphological host variables. However, a microscopic index to describe the degree of infection and colonization in the plant tissues has not yet been developed. A colonization or infection index would be a useful tool for studies that examine differences between individuals subjected to different treatments or to individuals belonging to different breeding accessions, together with their specific responses to the pathogen. This work presents a methodology based on the capture and digital treatment of microscopic images, using simple and accessible software, together with a range of variables that quantify the infection and colonization process. © 2012 Ruiz-Gómez et al.; licensee BioMed Central Ltd.


Hachero-Cruzado I.,Institute Investigacion Y Formacion Agraria Y Pesquera Of Andalucia Ifapa | Olmo P.,Institute Investigacion Y Formacion Agraria Y Pesquera Of Andalucia Ifapa | Sanchez B.,Institute Investigacion Y Formacion Agraria Y Pesquera Of Andalucia Ifapa | Herrera M.,Institute Investigacion Y Formacion Agraria Y Pesquera Of Andalucia Ifapa | Domingues P.,Spanish Institute of Oceanography
Aquaculture Research | Year: 2012

The effects of two diets with different lipid composition (squid Loligo gahi and one commercial pellet for breeders) on reproductive performance and egg lipid composition of brill (Scophthalmus rhombus) were compared. A total of 36 adult brill weighing 1482 ± 432 g were randomly distributed in the experimental tanks. A flow-through system composed of four tanks with 4.2 m2 of bottom area (5.0 m3 × 1.2 m water depth) was used. The experiment lasted for approximately 5 months (168 days). From the four mature females fed squid, three ovulated. Five females fed pellets matured and ovulated. Total ovulations were of 10 and 44, by the three females fed squid and the five ovulating female fed pellets respectively. Egg viability was higher, although not significantly, for females fed pellets. This indicates that pellets could be a better food for breeding females. In general, the egg total lipid content and the lipid classes composition did not seem to be affected by diet. In contrast, results indicate that not only diets but also individual females, regardless of the diet consumed, are determinant for egg fatty acid composition, clearly modulating their composition. © 2011 Blackwell Publishing Ltd.


Hachero-Cruzado I.,Institute Investigacion Y Formacion Agraria Y Pesquera Of Andalucia Ifapa | Fornies A.,Institute Ciencias Marinas Of Andalucia Icman | Herrera M.,Institute Investigacion Y Formacion Agraria Y Pesquera Of Andalucia Ifapa | Mancera J.M.,University of Cádiz | Martinez-Rodriguez G.,Institute Ciencias Marinas Of Andalucia Icman
Fish Physiology and Biochemistry | Year: 2013

The aims of the present study were to characterize sperm quality and to quantify seasonal changes in sexual hormone (testosterone [T], 11-ketotestosterone [11-KT] and 17,20β-dihydroxypregn-4-en-3-one [17,20β-P]) levels in male brill (Scophthalmus rhombus) plasma, as well as to test a more intensive sampling strategy to establish relationships between sex steroid levels and sperm production parameters. Sperm concentration ranged from 0. 5 to 3. 1 × 109 spermatozoa mL-1, and changes in sperm quality parameters depending on sampling date were observed. Plasma sexual steroid levels remained high and changed in parallel during the spawning season and afterwards decreased to very low levels in summer. The analysis of annual changes of 11-KT and T ratios suggests that 11-KT can be the main circulating androgen for stimulating spermatogenesis in S. rhombus and that T could be involved in the beginning of spermatogenesis through the positive feedback on brain-pituitary-gonad axis. Finally, daily 11-KT and T levels showed similar patterns of variation in males sampled, whereas 17,20β-P amounts showed somewhat opposite trends. These differences could be related with the different role of androgens and progestin during the spermatogenesis. © 2012 Springer Science+Business Media B.V.


Hermida M.,University of Santiago de Compostela | Rodriguez-Ramilo S.T.,University of Vigo | Rodriguez-Ramilo S.T.,Instituto Nacional Of Investigacion Y Tecnologia Agraria Y Alimentaria | Hachero-Cruzado I.,Institute Investigacion Y Formacion Agraria Y Pesquera Of Andalucia Ifapa | And 5 more authors.
Aquaculture | Year: 2014

Genetic maps constitute valuable tools to detect genomic regions associated with complex traits and to go forward to understand their genetic basis. Flatfish include several species of great commercial value for which increasing genomic resources are available including genetic maps and EST databases. Application of comparative mapping strategies to flatfish is relevant to obtain genetic information associated with productive traits. The brill (Scophthalmus rhombus) is a flatfish species closely related to turbot (S. maximus) whose meat is highly appreciated in the Spanish market. The Junta de Andalucía local Government has begun a program to adapt this species to captivity for its future production. In this study, we developed the first genetic map of brill using the current turbot genetic map as starting point. This strategy enabled us to select a number of homogeneously distributed markers in the turbot map and to apply cross-species microsatellite amplification to obtain informative markers. Nearly two hundred microsatellites from the framework turbot map were used for validation, and 100 markers were finally informative for mapping. The parents and offspring of the two families (54 and 88, respectively) used to construct the genetic map were genotyped with this panel. All markers, except eleven, were successfully grouped and ordered in 24 linkage groups. Linkage groups and order of markers were highly consistent with the previous turbot genetic map. Linkage map information was used to carry out a preliminary study on growth-related QTL for body weight, length and Fulton's condition factor in the two families, as the main phenotypic traits of interest in this species. © 2013 Elsevier B.V.


PubMed | Institute Investigacion Y Formacion Agraria Y Pesquera Of Andalucia Ifapa
Type: Journal Article | Journal: Plant methods | Year: 2012

Phytophthora cinnamomi Rands. is an important root rot pathogen widely distributed in the north hemisphere, with a large host range. Among others diseases, it is known to be a principal factor in the decline of holm oak and cork oak, the most important tree species in the dehesa ecosystem of south-western Spain. Previously, the focus of studies on P. cinnamomi and holm oak have been on molecular tools for identification, functional responses of the host, together with other physiological and morphological host variables. However, a microscopic index to describe the degree of infection and colonization in the plant tissues has not yet been developed. A colonization or infection index would be a useful tool for studies that examine differences between individuals subjected to different treatments or to individuals belonging to different breeding accessions, together with their specific responses to the pathogen. This work presents a methodology based on the capture and digital treatment of microscopic images, using simple and accessible software, together with a range of variables that quantify the infection and colonization process.

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