Bartolome A.,Complutense University of Madrid |
Bartolome A.,Research Center Biomedica En Red Of Diabetes fermedades Metabolicas Asociadas |
Bartolome A.,Institute Investigacion Sanitaria Del Hospital Clinico San Carlos Of Madrid |
Lopez-Herradon A.,Institute Investigacion Sanitaria IIS Fundacion Jimenez Diaz |
And 11 more authors.
Autophagy is a highly regulated homoeostatic process involved in the lysosomal degradation of damaged cell organelles and proteins. This process is considered an important pro-survival mechanism under diverse stress conditions. A diabetic milieu is known to hamper osteoblast viability and function. In the present study, we explored the putative protective role of autophagy in osteoblastic cells exposed to an HG (high glucose) medium. HG was found to increase protein oxidation and triggered autophagy by a mechanism dependent on reactive oxygen species overproduction in osteoblastic MC3T3-E1 cells. MC3T3-E1 cell survival was impaired by HG and worsened by chemical or genetic inhibition of autophagy. These findings were mimicked by H2O2-induced oxidative stress in these cells. Autophagy impairment led to both defective mitochondrial morphology and decreased bioenergetic machinery and inhibited further osteoblast differentiation in MC3T3-E1 cells upon exposure to HG. These novel findings indicate that autophagy is an essential mechanism to maintain osteoblast viability and function in an HG environment. © 2013 Biochemical Society. Source