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Diaz-Rubia L.,Intensive Care Unit | Ramos-Saez S.,Intensive Care Unit | Vazquez-Guillamet R.,University of New Mexico | Guerrero-Lopez F.,Intensive Care Unit | And 5 more authors.
Medicina Intensiva

Objective: To analyze the efficacy of negative fluid balance in hypoxemic patients with an elevated extravascular lung water index (EVLWI). Design: A retrospective observational study was made. Setting: Intensive Care Unit of Virgen de las Nieves Hospital (Spain). Participants: Forty-four patients participated in the study. Interventions: We analyzed our database of hypoxemic patients covering a period of 11 consecutive months. We included all hemodynamically stable and hypoxemic patients with EVLWI. >. 9. ml/kg. The protocol dictates a negative fluid balance between 500 and 1500. ml/day. We analyzed the impact of this negative fluid balance strategy upon pulmonary, hemodynamic, and renal function. Main variables of interest: Demographic data, severity scores, clinical, hemodynamic, pulmonary, metabolic and renal function data. Results: Thirty-three patients achieved negative fluid balance (NFB group) and 11 had a positive fluid balance (PFB group). In the former group, PaO2/FiO2 improved from 145 (IQR 106, 200) to 210mmHg (IQR 164, 248) (p<0.001), and EVLWI decreased from 14 (11, 18) to 10ml/kg (8, 14) (p<0.001). In the PFB group, EVLWI also decreased from 11 (10, 14) to 10ml/kg (8, 14) at the end of the protocol (p=0.004).For these patients there were no changes in oxygenation, with a PaO2/FiO2 of 216mmHg (IQR 137, 260) at the beginning versus 205mmHg (IQR 99,257) at the end of the study (p=0.08). Conclusion: Three out of four hypoxic patients with elevated EVLWI tolerated the NFB protocol. In these subjects, the improvement of various analyzed physiological parameters was greater and faster than in those unable to complete the protocol.Patients who did not tolerate the protocol were usually in more severe condition, though a larger sample would be needed to detect specific characteristics of this group. © 2014 Elsevier España, S.L.U. and SEMICYUC. Source

Garcia S.,Ugc Laboratorios Clinicos Hospital Universitario San Cecilio | de Haro T.,Ugc Laboratorios Clinicos Hospital Universitario San Cecilio | de Haro T.,Institute Investigacion Biosanitaria ibs | Zafra-Ceres M.,Ugc Laboratorios Clinicos Hospital Universitario San Cecilio | And 4 more authors.
International Journal of Medical Sciences

Background: Duchénnè/Becker muscular dystrophies (DMD/BMD) are X-linked diseases, which are caused by a de novo gene mutation in one-third of affected males. The study objectives were to determine the incidence of DMD/BMD in Andalusia (Spain) and to establish the percentage of affected males in whom a de novo gene mutation was responsible. Methods: Multiplex ligation-dependent probe amplification (MLPA) technology was applied to determine the incidence of DMD/BMD in 84 males with suspicion of the disease and 106 female relatives. Results: Dystrophin gene exon deletion (89.5%) or duplication (10.5%) was detected in 38 of the 84 males by MLPA technology; de novo mutations account for 4 (16.7%) of the 24 mother-son pairs studied. Conclusions: MLPA technology is adequate for the molecular diagnosis of DMD/BMD and establishes whether the mother carries the molecular alteration responsible for the disease, a highly relevant issue for genetic counseling. © Ivyspring International Publisher. Source

Fernandez-Gomez B.,Institute of Food Science Research CIAL CSIC UAM | Lezama A.,Institute of Food Science Research CIAL CSIC UAM | Amigo-Benavent M.,Institute of Food Science | Amigo-Benavent M.,University of Limerick | And 8 more authors.
Journal of Functional Foods

The bioaccessibility of chlorogenic acid (CGA) and caffeine in coffee silverskin extracts (CSE) and the contribution of these substances to the prophylactic effect of CSE on the pathogenesis of diabetes have not been reported. This study aimed to evaluate the bioaccessibility, bioavailability and bioactivity of CGA and caffeine alone and in CSE in the pancreas of rats treated with streptozotocin-nicotinamide (type 2 diabetes model). Metabolism of CGA and caffeine started in the gastrointestinal tract due to changes of pH taking place during digestion. Their metabolites protected pancreatic cells against the risk of diabetes. This is the first in vivo study to demonstrate a specific chemo-protective effect of CSE in pancreatic tissue, and this effect may be associated with its antioxidant capacity. Daily administration of CSE, CGA or caffeine 35 d previous to the induction of diabetes significantly reduced (p < 0.05) pancreatic oxidative stress and protein damage. © 2016 Elsevier Ltd. Source

Sanchez-Ojeda M.A.,University of Granada | De Luna-Bertos E.,University of Granada | De Luna-Bertos E.,Institute Investigacion Biosanitaria ibs
Nutricion Hospitalaria

The lifestyle is defined as the set of behavioral patterns and daily habits of a person, which maintained over time may become dimensions of risk or safety depending on their nature. The aim of this study was to know the lifestyles of university students in the following dimensions: diet, exercise, consumption of tobacco, alcohol and other drugs, sex and road safety. We made a literature review in electronic databases: PubMed, SCIELO and CUIDEN, between 2002-2014; using as keywords habits, lifestyle, health behaviors, young adult and university students. From articles found, stand out as most relevant data that university students have a high presence of favorable beliefs about healthy lifestyles and nevertheless not put into practice. We could conclude that according to different authors, university students in general have not a good eating habits, eating unbalanced diets high in calories. Besides the physical exercise is null, knowing that a good diet and doing exercise have beneficial effects on health. To this must be added the high consumption of alcohol, tobacco and marijuana among university students. © 2015 Grupo Aula Medica S.A. All rights reserved. Source

Rodriguez-Gomez R.,University of Granada | Jimenez-Diaz I.,University of Granada | Jimenez-Diaz I.,Institute Investigacion Biosanitaria ibs | Zafra-Gomez A.,University of Granada | Morales J.C.,Institute of Parasitology and Biomedicine Lopez Neyra

A simple and reliable method for the determination of the fructooligosaccharides (FOS) kestose (GF2), nystose (GF3), fructofuranosylnystose (GF4), in the presence of fructose, glucose and lactose in dairy products is proposed. The most relevant advantages of the proposed method are the simultaneous determination of the most common FOS in enriched products and a reduction of the time required for sample treatment since the method consists merely in addition of a precipitation solution for the removal of lipids and proteins. Furthermore, the method saves a substantial amount of reagents compared with other methods and sample manipulation is reduced. Two chromatographic separations are proposed. The first one is carried out on an amino phase column for liquid chromatography with refractive index detection (HPLC-RI) (concentration of analytes higher than 0.1 mg mL-1) and the second one on an anion-exchange Carbopac PA-1 column for high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) (concentration of FOS lower than 0.1 mg mL-1). The method was validated by recovery assays with spiked samples using matrix-matched calibration. The limits of quantification of the method ranged from 1.2 to 2.0 μg mL-1 for HPAEC-PAD and from 140 to 200 μg mL-1 for HPLC-RI, while inter- and intra-day variability was under 3.5% in all cases. The proposed method was applied to the determination of compounds in supplemented milk, infant formulas and milk related juices with good recoveries in all cases. © 2015 Elsevier B.V.All rights reserved. Source

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