Institute Investigacion Biosanitaria ibs

Granada, Spain

Institute Investigacion Biosanitaria ibs

Granada, Spain

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PubMed | Karolinska Institutet, Danish Cancer Society, University Paris Diderot, IDIBELL Catalan Institute of Oncology and 23 more.
Type: | Journal: International journal of cancer | Year: 2016

The association between H. pylori infection and pancreatic cancer risk remains controversial. We conducted a nested case-control study with 448 pancreatic cancer cases and their individually matched control subjects, based on the European Prospective Investigation into Cancer and Nutrition (EPIC) cohort, to determine whether there was an altered pancreatic cancer risk associated with H. pylori infection and chronic corpus atrophic gastritis. Conditional logistic regression models were applied to calculate odds ratios (ORs) and corresponding 95% confidence intervals (CIs), adjusted for matching factors and other potential confounders. Our results showed that pancreatic cancer risk was neither associated with H. pylori seropositivity (OR=0.96; 95% CI: 0.70, 1.31) nor CagA seropositivity (OR=1.07; 95% CI: 0.77, 1.48). We also did not find any excess risk among individuals seropositive for H. pylori but seronegative for CagA, compared with the group seronegative for both antibodies (OR=0.94; 95% CI: 0.63, 1.38). However, we found that chronic corpus atrophic gastritis was non-significantly associated with an increased pancreatic cancer risk (OR=1.35; 95% CI: 0.77, 2.37), and although based on small numbers, the excess risk was particularly marked among individuals seronegative for both H. pylori and CagA (OR=5.66; 95% CI: 1.59, 20.19, p value for interaction<0.01). Our findings provided evidence supporting the null association between H. pylori infection and pancreatic cancer risk in western European populations. However, the suggested association between chronic corpus atrophic gastritis and pancreatic cancer risk warrants independent verification in future studies, and, if confirmed, further studies on the underlying mechanisms. This article is protected by copyright. All rights reserved.

Ruiz-Ojeda F.J.,University of Granada | Ruiz-Ojeda F.J.,Institute Investigacion Biosanitaria ibs | Aguilera C.M.,University of Granada | Aguilera C.M.,Institute Investigacion Biosanitaria ibs | And 8 more authors.
Molecular and Cellular Endocrinology | Year: 2016

The present study was undertaken to investigate the effects of C-atrial natriuretic peptide (C-ANP4-23) in human adipose-derived stem cells differentiated into adipocytes over 10 days (1 μM for 4 h). The intracellular cAMP, cGMP and protein kinase A levels were determined by ELISA and gene and protein expression were determined by qRT-PCR and Western blot, respectively, in the presence or absence of C-ANP4-23. The levels of lipolysis and glucose uptake were also determined. C-ANP4-23 treatment significantly increased the intracellular cAMP levels and the gene expression of glucose transporter type 4 (GLUT4) and protein kinase, AMP-activated, alpha 1 catalytic subunit (AMPK). Western blot showed a significant increase in GLUT4 and phosphor-AMPKα levels. Importantly, the adenylate cyclase inhibitor SQ22536 abolished these effects. Additionally, C-ANP4-23 increased glucose uptake by 2-fold. Our results show that C-ANP4-23 enhances glucose metabolism and might contribute to the development of new peptide-based therapies for metabolic diseases. © 2016 Elsevier Ireland Ltd.

Fernandez-Gomez B.,Institute of Food Science Research CIAL CSIC UAM | Lezama A.,Institute of Food Science Research CIAL CSIC UAM | Amigo-Benavent M.,Institute of Food Science | Amigo-Benavent M.,University of Limerick | And 8 more authors.
Journal of Functional Foods | Year: 2016

The bioaccessibility of chlorogenic acid (CGA) and caffeine in coffee silverskin extracts (CSE) and the contribution of these substances to the prophylactic effect of CSE on the pathogenesis of diabetes have not been reported. This study aimed to evaluate the bioaccessibility, bioavailability and bioactivity of CGA and caffeine alone and in CSE in the pancreas of rats treated with streptozotocin-nicotinamide (type 2 diabetes model). Metabolism of CGA and caffeine started in the gastrointestinal tract due to changes of pH taking place during digestion. Their metabolites protected pancreatic cells against the risk of diabetes. This is the first in vivo study to demonstrate a specific chemo-protective effect of CSE in pancreatic tissue, and this effect may be associated with its antioxidant capacity. Daily administration of CSE, CGA or caffeine 35 d previous to the induction of diabetes significantly reduced (p < 0.05) pancreatic oxidative stress and protein damage. © 2016 Elsevier Ltd.

Sanchez-Ojeda M.A.,University of Granada | De Luna-Bertos E.,University of Granada | De Luna-Bertos E.,Institute Investigacion Biosanitaria ibs
Nutricion Hospitalaria | Year: 2015

The lifestyle is defined as the set of behavioral patterns and daily habits of a person, which maintained over time may become dimensions of risk or safety depending on their nature. The aim of this study was to know the lifestyles of university students in the following dimensions: diet, exercise, consumption of tobacco, alcohol and other drugs, sex and road safety. We made a literature review in electronic databases: PubMed, SCIELO and CUIDEN, between 2002-2014; using as keywords habits, lifestyle, health behaviors, young adult and university students. From articles found, stand out as most relevant data that university students have a high presence of favorable beliefs about healthy lifestyles and nevertheless not put into practice. We could conclude that according to different authors, university students in general have not a good eating habits, eating unbalanced diets high in calories. Besides the physical exercise is null, knowing that a good diet and doing exercise have beneficial effects on health. To this must be added the high consumption of alcohol, tobacco and marijuana among university students. © 2015 Grupo Aula Medica S.A. All rights reserved.

Garzon I.,University of Granada | Garzon I.,Institute Investigacion Biosanitaria ibs | Alfonso-Rodriguez C.A.,University of Granada | Alfonso-Rodriguez C.A.,Institute Investigacion Biosanitaria ibs | And 11 more authors.
Placenta | Year: 2014

Introduction: Human umbilical cord stem cells have inherent differentiation capabilities and potential usefulness in regenerative medicine. However, the epithelial differentiation capability and the heterogeneity of these cells have not been fully explored to the date. Methods: We analyzed the expression of several undifferentiation and epithelial markers in cells located in situ in different zones of the umbilical cord ein situ analysise and in primary ex vivo cell cultures of Wharton's jelly stem cells by microarray and immunofluorescence. Results: Our results demonstrated that umbilical cord cells were heterogeneous and had intrinsic capability to express in situ stem cell markers, CD90 and CD105 and the epithelial markers cytokeratins 3, 4, 7, 8, 12, 13, 19, desmoplakin and zonula occludens 1 as determined by microarray and immunofluorescence, and most of these markers remained expressed after transferring the cells from the in situ to the ex vivo cell culture conditions. However, important differences were detected among some cell types in the umbilical cord, with subvascular zone cells showing less expression of stem cell markers and cells in Wharton's jelly and the amnioblastic zones showing the highest expression of stem cells and epithelial markers. Conclusion: These results suggest that umbilical cord mesenchymal cells have intrinsic potential to express relevant epithelial markers, and support the idea that they could be used as alternative cell sources for epithelial tissue engineering. Copyright © 2014 Elsevier Ltd. All rights reserved.

Garcia S.,Ugc Laboratorios Clinicos Hospital Universitario San Cecilio | de Haro T.,Ugc Laboratorios Clinicos Hospital Universitario San Cecilio | de Haro T.,Institute Investigacion Biosanitaria ibs | Zafra-Ceres M.,Ugc Laboratorios Clinicos Hospital Universitario San Cecilio | And 4 more authors.
International Journal of Medical Sciences | Year: 2014

Background: Duchénnè/Becker muscular dystrophies (DMD/BMD) are X-linked diseases, which are caused by a de novo gene mutation in one-third of affected males. The study objectives were to determine the incidence of DMD/BMD in Andalusia (Spain) and to establish the percentage of affected males in whom a de novo gene mutation was responsible. Methods: Multiplex ligation-dependent probe amplification (MLPA) technology was applied to determine the incidence of DMD/BMD in 84 males with suspicion of the disease and 106 female relatives. Results: Dystrophin gene exon deletion (89.5%) or duplication (10.5%) was detected in 38 of the 84 males by MLPA technology; de novo mutations account for 4 (16.7%) of the 24 mother-son pairs studied. Conclusions: MLPA technology is adequate for the molecular diagnosis of DMD/BMD and establishes whether the mother carries the molecular alteration responsible for the disease, a highly relevant issue for genetic counseling. © Ivyspring International Publisher.

Diaz-Rubia L.,Virgen Of Las Nieves Hospital | Ramos-Saez S.,Virgen Of Las Nieves Hospital | Vazquez-Guillamet R.,University of New Mexico | Guerrero-Lopez F.,Virgen Of Las Nieves Hospital | And 5 more authors.
Medicina Intensiva | Year: 2015

Objective: To analyze the efficacy of negative fluid balance in hypoxemic patients with an elevated extravascular lung water index (EVLWI). Design: A retrospective observational study was made. Setting: Intensive Care Unit of Virgen de las Nieves Hospital (Spain). Participants: Forty-four patients participated in the study. Interventions: We analyzed our database of hypoxemic patients covering a period of 11 consecutive months. We included all hemodynamically stable and hypoxemic patients with EVLWI. >. 9. ml/kg. The protocol dictates a negative fluid balance between 500 and 1500. ml/day. We analyzed the impact of this negative fluid balance strategy upon pulmonary, hemodynamic, and renal function. Main variables of interest: Demographic data, severity scores, clinical, hemodynamic, pulmonary, metabolic and renal function data. Results: Thirty-three patients achieved negative fluid balance (NFB group) and 11 had a positive fluid balance (PFB group). In the former group, PaO2/FiO2 improved from 145 (IQR 106, 200) to 210mmHg (IQR 164, 248) (p<0.001), and EVLWI decreased from 14 (11, 18) to 10ml/kg (8, 14) (p<0.001). In the PFB group, EVLWI also decreased from 11 (10, 14) to 10ml/kg (8, 14) at the end of the protocol (p=0.004).For these patients there were no changes in oxygenation, with a PaO2/FiO2 of 216mmHg (IQR 137, 260) at the beginning versus 205mmHg (IQR 99,257) at the end of the study (p=0.08). Conclusion: Three out of four hypoxic patients with elevated EVLWI tolerated the NFB protocol. In these subjects, the improvement of various analyzed physiological parameters was greater and faster than in those unable to complete the protocol.Patients who did not tolerate the protocol were usually in more severe condition, though a larger sample would be needed to detect specific characteristics of this group. © 2014 Elsevier España, S.L.U. and SEMICYUC.

Rodriguez-Gomez R.,University of Granada | Jimenez-Diaz I.,University of Granada | Jimenez-Diaz I.,Institute Investigacion Biosanitaria Ibs | Zafra-Gomez A.,University of Granada | Morales J.C.,Institute of Parasitology and Biomedicine lopez Neyra
Talanta | Year: 2015

A simple and reliable method for the determination of the fructooligosaccharides (FOS) kestose (GF2), nystose (GF3), fructofuranosylnystose (GF4), in the presence of fructose, glucose and lactose in dairy products is proposed. The most relevant advantages of the proposed method are the simultaneous determination of the most common FOS in enriched products and a reduction of the time required for sample treatment since the method consists merely in addition of a precipitation solution for the removal of lipids and proteins. Furthermore, the method saves a substantial amount of reagents compared with other methods and sample manipulation is reduced. Two chromatographic separations are proposed. The first one is carried out on an amino phase column for liquid chromatography with refractive index detection (HPLC-RI) (concentration of analytes higher than 0.1 mg mL-1) and the second one on an anion-exchange Carbopac PA-1 column for high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) (concentration of FOS lower than 0.1 mg mL-1). The method was validated by recovery assays with spiked samples using matrix-matched calibration. The limits of quantification of the method ranged from 1.2 to 2.0 μg mL-1 for HPAEC-PAD and from 140 to 200 μg mL-1 for HPLC-RI, while inter- and intra-day variability was under 3.5% in all cases. The proposed method was applied to the determination of compounds in supplemented milk, infant formulas and milk related juices with good recoveries in all cases. © 2015 Elsevier B.V.All rights reserved.

Puche-Sanz I.,Institute Investigacion Biosanitaria ibs | Flores-Martin J.F.,Institute Investigacion Biosanitaria ibs | Vazquez-Alonso F.,Institute Investigacion Biosanitaria ibs | Exposito-Ruiz M.,Institute Investigacion Biosanitaria ibs | And 4 more authors.
Archivos Espanoles de Urologia | Year: 2016

OBJECTIVE: To compare the characteristics, clinical course, and survival of pairs of renal grafts from the same donor, with special interest in cold ischemia times (CIT) as a risk factor for graft survival. METHODS: We retrospectively reviewed paired grafts originating from the same cadaver donor from our prospectively recorded database of kidney transplants, from 1987 to 2015. We selected and divided them into two groups depending on whether they corresponded to the first or second graft. RESULTS: We studied a total of 860 paired kidneys. Mean CIT for the first and second groups were 15.12 and 19.16 hours, respectively. In the second group we observed higher incidences of acute tubular necrosis and initial delayed graft function (59.9% vs. 69.4% and 54.9% vs. 63.5%, respectively; p<0.001). No significant differences in either creatinine clearance rate or the rate of dialysis were observed between the two groups. No difference was found between the first and second groups in terms of graft survival (18.4 vs. 18.1 years, respectively; log-rank, p=0.667), and no differences were found by dividing the grafts into different categories according to their CIT (<14, 14-17, 17-20, >20 hours). For the set of grafts studied, CIT did not act as a risk factor for graft survival (hazard ratio [HR]=1.014; p=0.312). CONCLUSIONS: The proportion of ATN and DGF were greater in second transplants. However, there were no differences in long-term graft survival. Furthermore, we found no evidence that a CIT for less than 24 hours acted as a risk factor to graft survival.

PubMed | Hospital La Inmaculada, Servicio Of Urologia Del Hospital La Inmaculada and Institute Investigacion Biosanitaria IBS
Type: Journal Article | Journal: International braz j urol : official journal of the Brazilian Society of Urology | Year: 2016

The aim of this work is to study the density of the renal papillae in stone-forming patients and to determine its usefulness.This study included a total of 79 patients diagnosed with renal stones and on whom a computed tomography without contrast was performed from June 2014 to May 2015. The patients were divided into two groups: Group 1 (single episode) included 43 patients, and Group 2 (recurrent episodes) included 36 patients. The density of six renal papillae (3 per kidney) was measured, and the means obtained were compared between Groups 1 and 2. Statistical analysis was performed using SPSS 20.0.The mean papillary density in Group 1 was 32.26 (SD 4.07) HU compared to 42.36 (SD 8.03) HU in Group 2 (P=00001). A ROC curve was constructed, obtaining an optimal cut-off point of 36.8HU [area under the curve, 0.881 (95% CI; 0.804-0.958); P=0001], with a sensitivity of 80% and a specificity of 90%. The relative risk was estimated at 40.3 (95% CI; 10.8-151.1), meaning that a patient with a mean papillary density greater than 36.8HU would have a 40 times greater risk of having recurrent renal stones. The positive predictive value (PPV) was 81% and the negative predictive value (NPV) was 90%.The measurement of renal papillary density could be useful in predicting recurrent stone-formers. These results need to be confirmed in future studies with a greater number of patients and a longer follow-up.

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