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Barbarroja N.,University of Cambridge | Barbarroja N.,University of Cordoba, Spain | Rodriguez-Cuenca S.,University of Cambridge | Nygren H.,VTT Technical Research Center of Finland | And 17 more authors.
Diabetes | Year: 2015

Adipose tissue dysfunction is an important determinant of obesity-associated, lipid-induced metabolic complications. Ceramides are well-known mediators of lipidinduced insulin resistance in peripheral organs such as muscle. DEGS1 is the desaturase catalyzing the last step in the main ceramide biosynthetic pathway. Functional suppression of DEGS1 activity results in substantial changes in ceramide species likely to affect fundamental biological functions such as oxidative stress, cell survival, and proliferation. Here, we show that degs1 expression is specifically decreased in the adipose tissue of obese patients and murine models of genetic and nutritional obesity. Moreover, loss-of-function experiments using pharmacological or genetic ablation of DEGS1 in preadipocytes prevented adipogenesis and decreased lipid accumulation. This was associated with elevated oxidative stress, cellular death, and blockage of the cell cycle. These effects were coupled with increased dihydroceramide content. Finally, we validated in vivo that pharmacological inhibition of DEGS1 impairs adipocyte differentiation. These data identify DEGS1 as a new potential target to restore adipose tissue function and prevent obesity-associated metabolic disturbances. © 2015 by the American Diabetes Association.

Gomez-Zumaquero J.L.,Institute Investigacion Biomedica Of Malaga
Tissue Antigens | Year: 2013

Two novels alleles, HLA-A*01:128 and HLA-C*05:88 are characterized. © 2013 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

Rueda A.,Institute Investigacion Biomedica Of Malaga | Olmos D.,CSIC - National Center for Metallurgical Research | Vicioso L.,Hospital Universitario Virgen Of La Victoria | Quero C.,Hospital Universitario Virgen Of La Victoria | And 7 more authors.
Leukemia and Lymphoma | Year: 2015

We performed three different studies to obtain additional data on the biological and prognostic role of vascular endothelial growth factor C (VEGFC) in classical Hodgkin lymphoma (cHL): (1) serum VEGFC levels were measured by enzyme-linked immunosorbent assay in 80 patients; (2) immunohistochemical VEGFC expression in tumor tissue was evaluated using a case-control study in 62 patients; (3) gene expression of VEGFC was investigated in vitro in six cHL cell lines, and was compared with expression in inflammatory lymph nodes. Higher serum VEGFC levels were found in patients with cHL than in healthy volunteers (median, 7675 vs. 1358 pg/mL, p < 0.001) and high VEGFC expression in tissue predicted worse progression-free survival at 7 years (41% vs. 91%, p < 0.0001). VEGFC expression in all Hodgkin lymphoma cell lines was higher than the mean expression level in inflammatory lymph nodes. Our data suggest that Hodgkin and Reed-Sternberg cells produce VEGFC, and VEGFC expression could be a novel prognostic factor in cHL. © 2015 Informa UK, Ltd.

Bellon J.A.,University of Malaga | Bellon J.A.,Institute Investigacion Biomedica Of Malaga | Moreno-Peral P.,Institute Investigacion Biomedica Of Malaga | Moreno-Kustner B.,University of Malaga | And 12 more authors.
PLoS ONE | Year: 2014

Background: The predictD study developed and validated a risk algorithm for predicting the onset of major depression in primary care. We aimed to explore the opinion of patients about knowing their risk for depression and the values and criteria upon which these opinions are based. Methods: A maximum variation sample of patients was taken, stratified by city, age, gender, immigrant status, socioeconomic status and lifetime depression. The study participants were 52 patients belonging to 13 urban health centres in seven different cities around Spain. Seven Focus Groups (FGs) were given held with primary care patients, one for each of the seven participating cities. Results: The results showed that patients generally welcomed knowing their risk for depression. Furthermore, in light of available evidence several patients proposed potential changes in their lifestyles to prevent depression. Patients generally preferred to ask their General Practitioners (GPs) for advice, though mental health specialists were also mentioned. They suggested that GPs undertake interventions tailored to each patient, from a "patient-centred" approach, with certain communication skills, and giving advice to help patients cope with the knowledge that they are at risk of becoming depressed. Conclusions: Patients are pleased to be informed about their risk for depression. We detected certain beliefs, attitudes, values, expectations and behaviour among the patients that were potentially useful for future primary prevention programmes on depression. © 2014 Bellón et al.

Perez I.,Hospital Universitario Virgen Of La Victoria | Moreno T.,Hospital Universitario Virgen Of La Victoria | Navarro F.,Hospital Universitario Virgen Of La Victoria | Santos J.,Hospital Universitario Virgen Of La Victoria | And 3 more authors.
International Journal of STD and AIDS | Year: 2013

To determine the prevalence of erectile dysfunction and its associated factors we conducted an observational study on a consecutive cohort of asymptomatic HIV-positive men. All the patients completed a questionnaire to evaluate erectile dysfunction based on the International Index of Erectile Function, a validated survey for the diagnosis of anxiety and depression (self-administered HAD), and a questionnaire about cardiovascular risk factors. Epidemiological, clinical, and analytical data were collected. In all, 158 men, participated: mean age 46.0 years, 96.2% on antiretroviral therapy (91.3% undetectable viral load), and the mean CD4 count was 534 cells/mL. Erectile dysfunction was present in 106 (67.1%) patients, and associated factors were age (OR 4.5 for each 5 years; 95% CI 4.3-4.7; p = 0.0001) and anxiety (OR 8.2, 95% CI 2.2-30.4; p = 0.002). The prevalence of erectile dysfunction is high in men living with HIV, even in those with good immunovirological control. It is related to increasing age and anxiety, both of which are important factors within our HIV cohort. © The Author(s) 2013.

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