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Aparicio L.A.,Complejo Hospitalario Universitario runa | Abella V.,Institute Investigacion Biomedica A Coruna INIBIC | Valladares M.,Complejo Hospitalario Universitario runa | Figueroa A.,Institute Investigacion Biomedica A Coruna INIBIC
Cellular and Molecular Life Sciences | Year: 2013

Epithelial-to-mesenchymal transition (EMT), one of the crucial steps for carcinoma cells to acquire invasive capacity, results from the disruption of cell-cell contacts and the acquisition of a motile mesenchymal phenotype. Although the transcriptional events controlling EMT have been extensively studied, in recent years, several posttranscriptional mechanisms have emerged as critical in the regulation of EMT during tumor progression. In this review, we highlight the regulation of posttranscriptional events in EMT by RNA-binding proteins (RBPs). RBPs are responsible for controlling pre-mRNA splicing, capping, and polyadenylation, as well as mRNA export, turnover, localization, and translation. We discuss the most relevant aspects of RBPs controlling the metabolism of EMT-related mRNAs, and describe the implication of novel posttranscriptional mechanisms regulating EMT in response to different signaling pathways. Novel insight into posttranscriptional regulation of EMT by RBPs is uncovering new therapeutic targets in cancer invasion and metastasis. © 2013 The Author(s). Source

Barriales-Villa R.,Institute Investigacion Biomedica A Coruna INIBIC | Centurion-Inda R.,Institute Investigacion Biomedica A Coruna INIBIC | Fernandez-Fernandez X.,Institute Investigacion Biomedica A Coruna INIBIC | Ortiz M.F.,Institute Investigacion Biomedica A Coruna INIBIC | And 4 more authors.
Revista Espanola de Cardiologia | Year: 2010

The aims of this study were to determine the prevalence of severe cardiac conduction disturbances in a cohort of 451 patients with hypertrophic cardiomyopathy and to describe the characteristics of, and outcomes in, those who required a permanent pacemaker. A pacemaker was implanted in 48 patients (11%): 20 had sinus node dysfunction and 28 had an atrioventricular conduction disturbance. Primary bradyarrhythmia (which was not related to iatrogenic atrioventricular block or therapeutic ablation of the atrioventricular node) was the reason for permanent pacemaker implantation in 36 patients (8%). In 18% of cases, at least one other family member had a permanent pacemaker. In this patient series, a high prevalence of severe cardiac conduction disturbance leading to permanent pacemaker implantation was observed. Severe cardiac conduction disturbance in hypertrophic cardiomyopathy may also have a familial component. © 2010 Sociedad Española de Cardiología. Source

Cortes A.,University of Queensland | Hadler J.,University of Queensland | Pointon J.P.,National Health Research Institute | Robinson P.C.,University of Queensland | And 68 more authors.
Nature Genetics | Year: 2013

Ankylosing spondylitis is a common, highly heritable inflammatory arthritis affecting primarily the spine and pelvis. In addition to HLA-B*27 alleles, 12 loci have previously been identified that are associated with ankylosing spondylitis in populations of European ancestry, and 2 associated loci have been identified in Asians. In this study, we used the Illumina Immunochip microarray to perform a case-control association study involving 10,619 individuals with ankylosing spondylitis (cases) and 15,145 controls. We identified 13 new risk loci and 12 additional ankylosing spondylitis-associated haplotypes at 11 loci. Two ankylosing spondylitis-associated regions have now been identified encoding four aminopeptidases that are involved in peptide processing before major histocompatibility complex (MHC) class I presentation. Protective variants at two of these loci are associated both with reduced aminopeptidase function and with MHC class I cell surface expression. © 2013 Nature America, Inc. All rights reserved. Source

Rodriguez-Rigueiro T.,Institute Investigacion Biomedica A Coruna INIBIC | Valladares-Ayerbes M.,Institute Investigacion Biomedica A Coruna INIBIC | Valladares-Ayerbes M.,Complejo Hospitalario Universitario runa | Haz-Conde M.,Institute Investigacion Biomedica A Coruna INIBIC | And 8 more authors.
Proteomics | Year: 2011

Most of the archived pathological specimens in hospitals are kept as formalin-fixed paraffin-embedded tissues (FFPE) for long-term preservation. Up to now, these samples are only used for immunohistochemistry in a clinical routine as it is difficult to recover intact protein from these FFPE tissues. Here, we report a novel, short time-consuming and cost-effective method to extract full-length, non-degraded proteins from FFPE tissues. This procedure is combined with an effective and non-toxic deparaffinisation process and an extraction method based on antigen-retrieval, high concentration of SDS and high temperature. We have obtained enough intact protein to be detected by Western blotting analysis. This technique will allow utilising these stored FFPE tissues in several applications for protein analysis helping to advance the translational studies in cancer and other diseases. © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. Source

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