IICT Institute Investigacao Cientifica Tropical

Lisbon, Portugal

IICT Institute Investigacao Cientifica Tropical

Lisbon, Portugal
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Miller I.,University of Veterinary Medicine Vienna | Rogel-Gaillard C.,French National Institute for Agricultural Research | Spina D.,King's College London | Fontanesi L.,University of Bologna | And 4 more authors.
Current Protein and Peptide Science | Year: 2014

The rabbit (Oryctolagus cuniculus) is an important animal species widely used for biomedical research purposes, meat production and as a pet animal. There are numerous biomedical and scientific applications that include important areas such as antibody production, muscle, eye and circulatory physiology. The use of proteomics has been limited when considering this species. The aim of this article is to provide a review on applications of proteomics to the rabbit species, including those that are most relevant and where rabbit is a key species: muscle and circulatory system physiology. © 2014 Bentham Science Publishers.

Mau M.,University of Bonn | Mau M.,King's College London | de Almeida A.M.,New University of Lisbon | de Almeida A.M.,IICT Institute Investigacao Cientifica Tropical | And 2 more authors.
American Journal of Primatology | Year: 2011

Hamadryas baboons possess salivary proline-rich proteins (PRP), as indicated by the presence of pink-staining protein bands using 1D SDS gel electrophoresis and Coomassie R250 staining. The ability of these protein bands to interact with tannic acid was further examined. In a tannin-binding assay using 5μg tannic acid mixed with hamadryas whole saliva, we recently found four distinct protein bands of apparently 72, 55, 20, and 15kDa that were precipitated during the experiments. In this work, we were able to identify these protein bands in a follow-up analysis using MS/MS mass spectrometry after excising such bands out of air-dried gels. Albumin and α-amylase were present in the tannic acid-protein complexes, with albumin already known to nonspecifically interact with a great diversity of chemical compounds. More interesting, we also identified a basic PRP and a cystatin precursor protein. This was the first successful attempt to identify a PRP from precipitated tannin-protein complexes in hamadryas baboons using MS/MS mass spectrometry. On the other hand, the role of cystatins in tannin binding is not yet well understood. However, there are recent reports on cystatin expression in saliva of rats responding to astringent dietary compounds. In conclusion, the follow-up data on tannin-binding proteins present in salivary secretions from hamadryas baboons adds important knowledge to primate physiology and feeding ecology, in order to shed light on the establishment and development of food adaptations in primates. It also demonstrates that tannin binding is characteristic for PRP, but might not be restricted to this particular group of proteins in primate species. © 2011 Wiley-Liss, Inc..

Machado G.,University of Aveiro | Hladil J.,Academy of Sciences of the Czech Republic | Slavik L.,Academy of Sciences of the Czech Republic | Koptikova L.,Academy of Sciences of the Czech Republic | And 4 more authors.
Geologica Belgica | Year: 2010

An Emsian-Eifelian carbonate-volcaniclastic sequence in south-western Ossa-Morena Zone (Portugal) was studied in terms of reef fauna, conodont biostratigraphy, macro- and micro-facies and magnetic susceptibility stratigraphy. The results point to a bracketing between the Po. patulus and T. australis conodont biozones (uppermost Emsian - middle-late Eifelian). The field data, facies analysis and reef fauna indicate that the sequence is composed entirely of calciturbidite and debris-flow deposits (intercalated with hemipelagic tuffites) related to a (up-slope) reefal system resting on top of volcanic buildings within a large volcanic complex. The purity of the limestones does not seem to be generally influenced by volcanic contributions. Although with some uncertainties, the first part of the section seems to show pre-,syn- and post-Basal Choteč Event (BCE) beds as recorded by significant shifts in lithofacies and magnetic susceptibility signal. A tentative interregional correlation with magnetic susceptibility curves is suggested with sections in Morocco, Nevada (USA) and Uzbekistan.

Almeida A.M.,IICT Institute Investigacao Cientifica Tropical | Almeida A.M.,Ciisa Centro Interdisciplinar Of Investigacao Em Sanidade Animal | Almeida A.M.,New University of Lisbon | Almeida A.M.,IBET Institute Biologia Experimental e Tecnologica | And 9 more authors.
Journal of Proteomics | Year: 2014

Seasonal weight loss is the main limitation to animal production worldwide, significantly affecting the productivity of milk, meat and wool farms, particularly in drought-prone areas of the world where most of the large-scale wool production farms are located. Although the effect of nutritional status on wool quality parameters has been extensively studied, little is known on how it affects wool protein composition. Here, a proteomic approach has been applied to study changes in fiber structure and protein composition in wool from merino sheep subjected to experimentally induced weight loss. Results indicate that there is a significant reduction in the fiber diameter of wool from the animals on a restricted diet over a 42-day period. At the same time, significant increases in the expression of the high sulfur protein KAP13.1 and proteins from the high glycine-tyrosine protein KAP6 family in the wools from the animals on the restricted diet were also detected. Such findings have strong implications for the wool industry that favors finer wool. Biological significance: Seasonal weight loss caused by poor pasture availability has strong effects on wool productivity parameters and quality traits. In this work we determine that experimentally induced weight loss causes a decrease in fiber diameter associated with an increase in the level of high sulfur protein KAP13.1 and proteins from the high glycine-tyrosine protein KAP6 family. The implication of this is that decreasing the fiber diameter of the wool by this process could result in a fiber reduced prickle but with reduced wearability and appearance retention. © 2014 Elsevier B.V.

Paredi G.,University of Parma | Sentandreu M.-A.,CSIC - Institute of Agricultural Chemistry and Food Technology | Mozzarelli A.,University of Parma | Mozzarelli A.,Italian National Institute of Biosystems and Biostructures | And 4 more authors.
Journal of Proteomics | Year: 2013

Meat consumption is an important part of human diet with strong implications in health, economy and culture worldwide. Meat is a proteinaceous product and therefore proteomics holds a considerable value to the study of the protein events underlying meat production and processing. In this article we will review this subject in an integrated "farm to fork" perspective, i.e. focusing on all the major levels of the meat producing chain: farm, abattoir and transformation industry. We will focus on the use, importance and applications of proteomics, providing clear examples of the most relevant studies in the field. A special attention will be given to meat production, as well as quality control. In the latter, a particular emphasis will be given to microbial safety and the detection of frauds.This article is part of a Special Issue entitled: New Horizons and Applications for Proteomics [EuPA 2012]. © 2013 Elsevier B.V.

Martins L.L.,University of Lisbon | Mourato M.P.,University of Lisbon | Baptista S.,University of Lisbon | Reis R.,University of Lisbon | And 6 more authors.
Acta Physiologiae Plantarum | Year: 2014

The response of tobacco plants genetically engineered with the AtTPS1 gene to stress induced by excess Cu and Cd was evaluated in hydroponic solution (100 and 400 μM Cu and 50 and 200 μM Cd) after a 48 h exposure. Two transgenic lines, transformed with the AtTPS1 (trehalose-6-phosphate synthase) gene from Arabidopsis, with different levels of trehalose-6-phosphate synthase expression (B5H, higher and B1F, lower), and a wild type (WT) were investigated. Protein content, antioxidative enzymes (CAT, POD, SOD, and APX), glucose, fructose, lipid peroxidation, hydrogen peroxide and Cd and Cu contents were determined in leaves. The two transgenic lines were differently influenced by Cd and Cu exposure as they induced a different antioxidant enzymatic defense response. B1F and B5H plants showed a better acclimation to Cd and excess Cu compared to WT. Furthermore B1F was more tolerant than B5H to Cd and excess Cu. B1F accumulated less Cd and Cu in leaves, probably due to a more efficient exclusion mechanism. Catalase was shown to be the most important enzyme in the antioxidative system of these plants. © 2013 Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków.

Almeida A.M.,IICT Institute Investigacao Cientifica Tropical | Almeida A.M.,Ciisa Centro Interdisciplinar Of Investigacao Em Sanidade Animal | Campos A.,New University of Lisbon | Francisco R.,New University of Lisbon | And 6 more authors.
Animal Genetics | Year: 2010

The study of changes within the key agents regulating metabolism during genetic upgrading because of selection can contribute to an improved understanding of genomic and physiological relationships. This may lead to increased efficiencies in animal production. These changes, regarding energy and protein metabolic saving mechanisms, can be highlighted during food restriction periods. In this study, a 20% weight reduction was induced in two rabbit breeds: New Zealand white, a selected meat producer (Oryctolagus cuniculus cuniculus), and Iberian wild rabbit (Oryctolagus cuniculus algirus), with the aim of determining differential protein expression in the gastrocnemius muscle within control (ad libitum) and restricted diet experimental animal groups, using techniques of two-dimensional gel electrophoresis and peptide mass fingerprinting. Results show that l-lactate dehydrogenase, adenylate kinase, β enolase and α enolase, fructose bisphosphate aldolase A and glyceraldehyde 3-phosphate dehydrogenase, which are enzymes involved in energy metabolism, are differentially expressed in restricted diet experimental animal groups. These enzymes are available to be further tested as relevant biomarkers of weight loss and putative objects of manipulation as a selection tool towards increasing tolerance to weight loss. Similar reasoning could be applied to 2D gel electrophoresis spots corresponding to the important structural proteins tropomyosin β chain and troponin I. Finally, a spot identified as mitochondrial import stimulation factor seems of special interest as a marker of undernutrition, and it may be the object of further studies aiming to better understand its physiological role. © 2009 Stichting International Foundation for Animal Genetics.

Belini U.L.,University of Sao Paulo | Hein P.R.G.,CIRAD - Agricultural Research for Development | Filho M.T.,University of Sao Paulo | Rodrigues J.C.,IICT Institute Investigacao Cientifica Tropical | Chaix G.,CIRAD - Agricultural Research for Development
BioResources | Year: 2011

Medium density fiberboard (MDF) is an engineered wood product formed by breaking down selected lignin-cellulosic material residuals into fibers, combining it with wax and a resin binder, and then forming panels by applying high temperature and pressure. Because the raw material in the industrial process is ever-changing, the panel industry requires methods for monitoring the composition of their products. The aim of this study was to estimate the ratio of sugarcane (SC) bagasse to Eucalyptus wood in MDF panels using near infrared (NIR) spectroscopy. Principal component analysis (PCA) and partial least square (PLS) regressions were performed. MDF panels having different bagasse contents were easily distinguished from each other by the PCA of their NIR spectra with clearly different patterns of response. The PLS-R models for SC content of these MDF samples presented a strong coefficient of determination (0.96) between the NIR-predicted and Lab-determined values and a low standard error of prediction (~1.5%) in the cross-validations. A key role of resins (adhesives), cellulose, and lignin for such PLS-R calibrations was shown. PLS-DA model correctly classified ninety-four percent of MDF samples by cross-validations and ninety-eight percent of the panels by independent test set. These NIR-based models can be useful to quickly estimate sugarcane bagasse vs. Eucalyptus wood content ratio in unknown MDF samples and to verify the quality of these engineered wood products in an online process.

Araujo R.,New University of Lisbon | Casanova C.,New University of Lisbon | Casanova C.,IICT Institute Investigacao Cientifica Tropical | Melo M.J.,New University of Lisbon | Lemos A.,New University of Lisbon
European Journal of Science and Theology | Year: 2015

This paper discusses the ethical approach and decision making for the conservation of a French book of hours (cofre no.24), currently stored in the 18th century library of Palácio Nacional de Mafra (PNM), in Lisbon, Portugal. This book of hours, named cofre no.24, has the manuscript’s original text block dated from ca. 1420 and three folios were added later, in the second half of the 15th century. The conservation decision was taken based on the study and full comprehension of the techniques and materials present in the book and bearing in mind the significance and the role of this symbolic book, throughout its life. The main aspects of diagnosis of the condition of the book are presented and the conservation treatment briefly described. The colours analysed reveal the artist’s original trace and no other alteration, reinforcing the proposed minimal intervention for the text block. As opposed, the binding of the late 18th/early 19th centuries was in very poor condition, requiring the rebinding of the whole book. Treatment decisions are raised and discussed, namely the choice of maintaining the binding found versus recovering old bookbinding features, as the main theme of an ongoing research. © 2015, Ecozone, OAIMDD.

PubMed | IICT Institute Investigacao Cientifica Tropical, University of Western Australia and Government of Western Australia
Type: Journal Article | Journal: Journal of the science of food and agriculture | Year: 2016

Muscle fatty acid profile reflects the body condition of animals and has a noticeable effect on meat quality. Herein, longissimus dorsi muscle of three different sheep breeds, Damara (a fat-tailed breed), Dorper and Australian Merino sheep, was analysed for fatty acid composition. The three breeds were subjected to two distinctive feeding levels (ad libitum and restricted feeding) over 42 days.The Damara sheep revealed several differences compared to the other two breeds, namely a higher concentration of polyunsaturated fatty acids, which can be related to being a fat-tailed breed. Even in restricted feeding conditions, this breed revealed the highest levels compared to Merino and Dorper sheep respectively, of linoleic acid (+31% and +28%), linolenic acid (+97% and +51%), eicosapentaenoic acid (EPA) (+65% and +37%), docosapentanenoic acid (DPA) (+31% Merino) and dodosahexanenoic acid (DHA) (+63% and +77%). EPA, DPA and DHA are three omega-3 fatty acids, with described beneficial characteristics.With this work we show other qualities (higher levels of the omega-3 fatty acids, EPA, DPA and DHA) of Damara meat that might present this breed as an interesting alternative for animal production in semi-arid climates.

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