Institute Investigacao Cientifica do Parana

Cascavel, Brazil

Institute Investigacao Cientifica do Parana

Cascavel, Brazil
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Aigner C.P.,Paranaense University | Aigner C.P.,Institute Investigacao Cientifica do Parana | da Silva A.V.,State University of Feira de Santana | Sandrini F.,Institute Investigacao Cientifica do Parana | And 3 more authors.
Memorias do Instituto Oswaldo Cruz | Year: 2010

This study aimed to quantify Toxoplasma gondii in tissue samples of serologically positive chickens using real-time polymerase chain reaction (PCR). Of 65 chickens evaluated, 28 were positive for T. gondii antibodies. Brain and heart samples were collected from 26 seropositive chickens and DNA was extracted using Trizol® and amplified using real-time PCR with SYBR® Green. Parasite DNA was detected in 24 of the 26 samples analyzed; the number of positive tissue samples and the parasite quantity did not differ between tissue types. The results confirmed the analytical sensitivity of parasite detection in chicken tissue samples and demonstrated the possibility of using other molecular systems for genotypic analysis.

de Assis Poiares L.,Institute Investigacao Cientifica do Parana | de Sa Osorio P.,Institute Investigacao Cientifica do Parana | Gusmao L.,University of Porto | Largura A.,Institute Investigacao Cientifica do Parana | And 2 more authors.
Forensic Science International: Genetics | Year: 2010

Allele frequencies for 15 short tandem repeat (STR) loci were obtained from a sample of 12,030 individuals undergoing paternity testing. This sample includes individuals from all States in Brazil, combined according to the current country division into five regions (North, Northeast, Central West, Southeast, and South). The most polymorphic loci were D2S1338 and D18S51. All the analysed loci meet Hardy-Weinberg equilibrium expectations. Combined power of discrimination and combined power of exclusion for the 15 tested STR loci were 0.999999999999990 and 0.9999992, respectively. Comparative analysis between populations from different Brazilian macroregions as well as between Brazil and other relevant populations are presented. © 2009 Elsevier Ireland Ltd. All rights reserved.

Dalanhol M.,Setor Of Hematologia E Citometria Of Fluxo Do Laboratorio Alvaro | Barros M.,West Parana State University | Mazuchelli J.,State University of Maringá | Silva P.H.,SBAC | And 2 more authors.
Revista Brasileira de Hematologia e Hemoterapia | Year: 2010

This study evaluated possible alterations in different hematologic parameters [total erythrocyte count, hematocrit, hemoglobin concentration, mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MHCM), total leukocyte count and platelet count], of blood samples submitted to varied storage times at both 4°C and at room temperature. The analyses were performed using a Sysmex XT2000i automated hematology analyzer. Written consent was obtained from donors with no hematological disorders or clinical abnormalities and thus from individuals with apparently normal laboratory reference values for healthy adults. The results were analysed by descriptive statistics with comparisons of the means being achieved by analysis of variance (ANOVA). The means of the MCHC parameters and platelet count showed significant statistical differences for both storage temperatures. At room temperature the hematocrit, MCV and red cell distribution width (RDW) readings also presented statistically significant differences. Therefore, it can be concluded that hemogram results performed by automated blood analyzers are satisfactory when carried out within 24 hours after drawing the blood sample for most of the parameters evaluated.

Lourenco E.S.O.,UNIAMERICA e UDC | Cardoso D.L.,West Parana State University | Mateus F.H.,Institute Investigacao Cientifica do Parana
Engenharia Agricola | Year: 2010

This research had as objective to evaluate the potential of permeable reactive barriers in the reduction of aromatical hydrocarbons BTEX (Benzene, Toluene, Ethylbenzene and Xylenes) in the ground, coming from gasoline leak. The experiment was carried out in a laboratorial scale, simulating two types of PRB called: AS (H2O2+ soil) and ACA (H2O2+ activated coal). The BTEX concentrations in the gasoline sample were assessed when entering in PRB and in the percolate in pre-established retention times of 24; 36; 48; 60; 72 and 84h, using gas chromatography. The obtained results showed that the two reactive barriers reduced the BTEX concentration, close at permissible levels of contamination and prevention, and the ACA barrier presented better results.

De Andrade M.P.,University of Sao Paulo | Hirata R.D.C.,University of Sao Paulo | Sandrini F.,Institute Investigacao Cientifica do Parana | Largura A.,Institute Investigacao Cientifica do Parana | Hirata M.H.,University of Sao Paulo
Clinical Chemistry and Laboratory Medicine | Year: 2012

Background: The biorhythm of serum uric acid was evaluated in a large sample of a clinical laboratory database by spectral analysis and the influence of the gender and age on uric acid variability. Methods: Serum uric acid values were extracted from a large database of a clinical laboratory from May 2000 to August 2006. Outlier values were excluded from the analysis and the remaining data (n = 73,925) were grouped by gender and age ranges. Rhythm components were obtained by the Lomb Scargle method and Cosinor analysis. Results: Serum uric acid was higher in men than in women older than 13 years (p <0.05). Compared with 0-12 year group, uric acid increased in men but not in women older than 13 years (p <0.05). Circannual (12 months) and trans-year (17 months) rhythm components were detected, but they were significant only in adult individuals (> 26 years, p <0.05). Cosinor analysis showed that midline estimating statistic of rhythm (MESOR) values were higher in men (range:353-368 μ mol/L) than in women (range:240-278 μmol/L; p <0.05), independent of the age and rhythm component. The extent of predictable change within a cycle, approximated by the double amplitude, represented up to 20 % of the corresponding MESOR. Conclusions: Serum uric acid biorhythm is dependent on gender and age and it may have relevant influence on preana-lytical variability of clinical laboratory results. © 2012 by Walter de Gruyter • Berlin • Boston.

Tonial G.C.,Federal University of Santa Catarina | Passos A.M.,Federal University of Santa Catarina | do Livramento A.,Federal University of Santa Catarina | Scaraveli N.G.,Federal University of Santa Catarina | And 5 more authors.
Revista da Sociedade Brasileira de Medicina Tropical | Year: 2011

Introduction: Hepatitis B infection constitutes an important cause of morbidity and mortality worldwide. In Brazil, however, the current epidemiological situation is not clear. Considering the importance of establishing this prevalence, the aim of this study was to determine the prevalence of HBV markers in voluntary adolescents, junior high (secondary school) students, in the city of Itajaí, State of Santa Catarina, Brazil. Methods: A seroepidemiological, transverse study was conducted with 353 randomly chosen adolescents from elementary school in 2008. Blood samples were analyzed for HBsAg, anti-HBc and anti-HBs. All analyses were conducted by automated microparticle enzyme immunosorbent assay (Abbott®, AxSYM system, Deerfield, IL, USA), according to the manufacturer's instructions. Results: The prevalence of HBsAg was 0.6% (CI 95% 0.1 - 2.0), that of anti-HBc was 1.1% (CI 95% 0.3 - 2.9) and that of detectable anti-HBs was 83.6% (CI 95% 79.3 - 87.3). Hepatitis B vaccination coverage was 97.5% (CI 95% 95.2 - 98.8). Conclusions: These results demonstrate the success of the vaccination program against hepatitis B in the region studied and indicate that prevention strategies must be maintained and, if possible, expanded to contribute to the establishment of positive prevalence rates in all age groups.

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