Time filter

Source Type

Chakraborty D.,Institute of Integrated Study | Chakraborti S.,Institute of Integrated Study
Research Journal of Phytochemistry

The antibacterial activity of methanolic extract of green tea leaves (Camellia sinensis; Family: Theaceae) was studied on four different bacteria namely Escherichia coli, Bacillus cereus, Pseudomonas aeruginosa and Staphylococcus aureus and two fungi of Aspergillus species. Antibacterial activity was assigned by measuring the Zone of Inhibition (ZOI) as well as Minimum Inhibitory Concentration (MIC) at four different concentrations of the methanolic extract (10, 25, 50 and 100 mg mL-1) and erythromycin (10 mg mL-1) which was taken as the antibiotic control. Preliminary screening was done to find out the phytochemical components present in the extract. Thin Layer Chromatography (TLC) was performed to separate and isolate the bioactive components present in the extract. Further bioassay-guided fractionation of the methanolic extract with the help of column chromatography was performed using a stepwise gradient with Chloroform: Methanol against the bacteria. The fraction which gave us the best result was further taken for the FJPTLC for the identification of the bioactive compound which was identified as catechin. Zone of inhibition was observed in most of the organism. Alcoholic extract of the leaves of Camellia sinensis was found to be most effective against Bacillus cereus. © 2010 Academic Journals Inc. Source

Discover hidden collaborations