Time filter

Source Type

Palomares-Rius F.J.,Institute Hortofruticultura Subtropical y Mediterranea la Mayora IHSM | Viruel M.A.,Institute Hortofruticultura Subtropical y Mediterranea la Mayora IHSM | Yuste-Lisbona F.J.,Institute Hortofruticultura Subtropical y Mediterranea la Mayora IHSM | Yuste-Lisbona F.J.,University of Almeria | And 2 more authors.
Theoretical and Applied Genetics | Year: 2011

A population of recombinant inbred lines (RIL) derived from a cross between the Watermelon mosaic virus (WMV) resistant genotype TGR-1551 and the susceptible Spanish cultivar 'Bola de Oro' has been evaluated for WMV resistance in spring, fall and growth chamber conditions. The quantitative trait loci (QTL) analyses detected one major QTL (wmv) on linkage group (LG) XI close to the microsatellite marker CMN04_35. This QTL controls the resistance to WMV in the three environmental conditions evaluated. Other minor QTLs affecting the severity of viral symptoms were identified, but they were not detected in all the assayed environments. The screening of the marker CMN04_35 in an F 2 progeny, derived from the same cross, confirmed the effect of this QTL on the expression of WMV resistance also in early generations, which evidences the usefulness of this marker for a marker assisted selection program. © 2011 Springer-Verlag. Source

Perez V.,Institute Hortofruticultura Subtropical y Mediterranea la Mayora IHSM | Hormaza J.I.,Institute Hortofruticultura Subtropical y Mediterranea la Mayora IHSM | Herrero M.,CSIC - Aula Dei Experimental Station
Acta Horticulturae | Year: 2015

The importance of mango growing contrasts with the paucity of information on its reproductive biology. In this work, the reproductive biology of mango was studied in the subtropical conditions of the Southern Spanish Mediterranean coast, using the cultivars 'Osteen', 'Kensington', 'Kent' and 'Keitt'. In order to establish the appropriate developmental coordinates during the reproductive phase, phenology was studied from the start of the blooming season to fruit harvest. Also to detect the reasons behind the low fruit set in mango, pollen tube pathway and fertilization were analysed, using microscopy techniques. A good proportion of the flowers were male and dropped soon after anthesis. Pollen grains readily germinated, pollen tubes reached the base of the style within one day, penetrated the ovule through the nucellus and reached the embryo sac three days after pollination. Endosperm nuclei soon developed, but the embryo remained in a quiescent stage. A good proportion of the ovules degenerated at different stages of development. Temperature had an effect on pollen performance, and low temperatures hampered pollen germination. Finally, a compatibility assay was performed, showing no differences between self and cross pollen tube growth in the style. However a paternity analysis, with microsatellite markers, showed significant differences in outcrossing rate depending on the availability of pollinizer trees. Source

Salinas M.,University of Almeria | Capel C.,University of Almeria | Alba J.M.,Institute Hortofruticultura Subtropical y Mediterranea la Mayora IHSM | Mora B.,University of Almeria | And 4 more authors.
Theoretical and Applied Genetics | Year: 2013

A novel source of resistance to two-spotted spider mite (Tetranychus urticae Koch) was found in Solanum pimpinellifolium L. accession TO-937 and thereby a potential source of desirable traits that could be introduced into new tomato varieties. This resistance was found to be controlled by a major locus modulated by minor loci of unknown location in the genome of this wild tomato. We first applied a bulked segregant analysis (BSA) approach in an F4 population as a method for rapidly identifying a genomic region of 17 cM on chromosome 2, flanked by two simple sequence repeat markers, harboring Rtu2. 1, one of the major QTL involved in the spider mite resistance. A population of 169 recombinant inbred lines was also evaluated for spider mite infestation and a highly saturated genetic map was developed from this population. QTL mapping corroborated that chromosome 2 harbored the Rtu2. 1 QTL in the same region that our previous BSA findings pointed out, but an even more robust QTL was found in the telomeric region of this chromosome. This QTL, we termed Rtu2. 2, had a LOD score of 15. 43 and accounted for more than 30 % of the variance of two-spotted spider mite resistance. Several candidate genes involved in trichome formation, synthesis of trichomes exudates and plant defense signaling have been sequenced. However, either the lack of polymorphisms between the parental lines or their map position, away from the QTL, led to their rejection as candidate genes responsible for the two-spotted spider mite resistance. The Rtu2 QTL not only serve as a valuable target for marker-assisted selection of new spider mite-resistant tomato varieties, but also as a starting point for a better understanding of the molecular genetic functions underlying the resistance to this pest. © 2012 Springer-Verlag. Source

Rodriguez-Lopez M.J.,Institute Hortofruticultura Subtropical y Mediterranea la Mayora IHSM | Garzo E.,CSIC - Center for Environmental Sciences | Bonani J.P.,CSIC - Center for Environmental Sciences | Fereres A.,CSIC - Center for Environmental Sciences | And 2 more authors.
Phytopathology | Year: 2011

Breeding of tomato genotypes that limit whitefly (Bemisia tabaci) access and feeding might reduce the spread of Tomato yellow leaf curl virus (TYLCV), a begomovirus (genus Begomovirus, family Geminiviridae) that is the causal agent of tomato yellow leaf curl disease. TYLCV is restricted to the phloem and is transmitted in a persistent manner by B. tabaci. The tomato breeding line ABL 14-8 was developed by introgressing type IV leaf glandular trichomes and secretion of acylsucroses from the wild tomato Solanum pimpinellifolium accession TO-937 into the genetic background of the whitefly-and virus-susceptible tomato cultivar Moneymaker. Results of preference bioassays with ABL 14-8 versus Moneymaker indicated that presence of type IV glandular trichomes and the production of acylsucrose deterred the landing and settling of B. tabaci on ABL 14-8. Moreover, electrical penetration graph studies indicated that B. tabaci adults spent more time in nonprobing activities and showed a reduced ability to start probing. Such behavior resulted in a reduced ability to reach the phloem. The superficial type of resistance observed in ABL 14-8 against B. tabaci probing significantly reduced primary and secondary spread of TYLCV. © 2011 The American Phytopathological Society. Source

Yuste-Lisbona F.J.,Institute Hortofruticultura Subtropical y Mediterranea la Mayora IHSM | Yuste-Lisbona F.J.,University of Almeria | Capel C.,University of Almeria | Gomez-Guillamon M.L.,Institute Hortofruticultura Subtropical y Mediterranea la Mayora IHSM | And 3 more authors.
Theoretical and Applied Genetics | Year: 2011

Powdery mildew caused by Podosphaera xanthii is a major disease in melon crops, and races 1, 2, and 5 of this fungus are those that occur most frequently in southern Europe. The genotype TGR-1551 bears a dominant gene that provides resistance to these three races of P. xanthii. By combining bulked segregant analysis and amplified fragment length polymorphisms (AFLP), we identified eight markers linked to this dominant gene. Cloning and sequencing of the selected AFLP fragments allowed the development of six codominant PCR-based markers which mapped on the linkage group (LG) V. Sequence analysis of these markers led to the identification of two resistance-like genes, MRGH5 and MRGH63, belonging to the nucleotide binding site (NBS)-leucine-rich repeat (LRR) gene family. Quantitative trait loci (QTL) analysis detected two QTLs, Pm-R1-2 and Pm-R5, the former significantly associated with the resistance to races 1 and 2 (LOD score of 26.5 and 33.3; 53.6 and 61.9% of phenotypic variation, respectively), and the latter with resistance to race 5 (LOD score of 36.8; 65.5% of phenotypic variation), which have been found to be colocalized with the MRGH5 and MRGH63 genes, respectively. The results suggest that the cluster of NBS-LRR genes identified in LG V harbours candidate genes for resistance to races 1, 2, and 5 of P. xanthii. The evaluation of other resistant germplasm showed that the codominant markers here reported are also linked to the Pm-w resistance gene carried by the accession 'WMR-29' proving their usefulness as genotyping tools in melon breeding programmes. © 2011 Springer-Verlag. Source

Discover hidden collaborations