Institute Ganaderia Of Montana Csic Ule

Leon, Spain

Institute Ganaderia Of Montana Csic Ule

Leon, Spain
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Bichi E.,Institute Ganaderia Of Montana Csic Ule | Bichi E.,French National Institute for Agricultural Research | Toral P.G.,Institute Ganaderia Of Montana Csic Ule | Hervas G.,Institute Ganaderia Of Montana Csic Ule | And 5 more authors.
Journal of Dairy Science | Year: 2012

This study was conducted in lactating ewes to examine the involvement of {increment} 9-desaturase in mammary lipogenesis, especially in the endogenous synthesis of cis-9, trans-11 18:2 and cis-9 18:1, because no information on this matter was available for dairy sheep. With this aim, 6 Assaf ewes were monitored in a 15-d experiment, which included a 5-d pretreatment period, a 5-d treatment period, and a 5-d posttreatment period. During the treatment period, ewes received 0.5g/d of sterculic acid (a cyclopropene fatty acid that inhibits {increment} 9-desaturase), delivered intravenously in 4 equal doses at 6-h intervals. Animals were fed pasture to supply mainly α-linolenic acid and minimize the amount of milk cis-9, trans-11 18:2 of ruminal origin. Sterculic acid administration was calculated to inhibit {increment} 9-desaturase by 70% based on the milk content of cis-9 14:1. This inhibition resulted in decreases in the milk content of the enzyme products (e.g., cis-9 10:1, cis-9 14:1, cis-9 16:1, cis-9 18:1, and cis-9, trans-11 18:2) and increases in its substrates (e.g., 14:0, 18:0, and trans-11 18:1), as well as in reductions in the desaturase indexes. Some other milk fatty acids, further to previously reported products or substrates of {increment} 9-desaturase (e.g., cis-15 18:1 and cis-9, cis-15 18:2, or trans-11, trans-15 18:2, and cis-9, trans-11, trans-15 18:3), were also affected by sterculic acid administration. Endogenous synthesis was the major source of cis-9 18:1 and cis-9, trans-11 18:2, accounting for 63 and 74% of its content in milk fat, respectively. To our knowledge, the present study provides the first estimates of endogenous synthesis of these 2 bioactive fatty acids in ovine milk fat. © 2012 American Dairy Science Association.


Gomez-Cortes P.,Institute Investigacion En Ciencias Of La Alimentacion Csic Uam | Gallardo B.,University of Valladolid | Mantecon A.R.,Institute Ganaderia Of Montana Csic Ule | Juarez M.,Institute Investigacion En Ciencias Of La Alimentacion Csic Uam | And 2 more authors.
Meat Science | Year: 2014

The main objective of this study was to evaluate the effects of supplementing lactating ewe diets with extruded linseed on the fatty acid (FA) composition of intramuscular and subcutaneous fat depots of suckling lambs. Twenty-four pregnant Churra ewes were divided into two groups based on the milk production, age, body weight and parity, and assigned to one of two treatments. Each ewe of the Control treatment was supplemented with 70. g/day of FAs from a calcium soap of palm oil, while the other treatment group (Lin) was supplemented with 128. g/day of extruded linseed. All lambs were reared exclusively on milk and were slaughtered when they reached 11. kg live weight. FA profiles of ewe milk, lamb meat and subcutaneous adipose tissue were determined by GC. Lamb performance was not affected by the treatments. Muscle fat and adipose tissue from the Lin treatment showed higher proportions of polyunsaturated fatty acids (PUFA). The percentages of α-linolenic (C18:3 n.3), docosahexaenoic (C22:6 n. 3), vaccenic (trans-11 C18:1) and rumenic (cis-9, trans-11 C18:2) acids in both fat depots were higher in Lin than in Control suckling lambs. Furthermore, meat fat from Lin carcasses displayed a lower n. 6/n. 3 ratio than Control samples. Intramuscular depots clearly showed a greater content of PUFA, including cis-9, trans-11 C18:2, and a lower n. 6/n. 3 ratio than subcutaneous fat. The results from this study demonstrate that dietary extruded linseed supplementation of lactating ewes enhances the nutritional quality of suckling lamb fat depots such as intramuscular and subcutaneous fats. © 2013 Elsevier Ltd.


Regidor-Cerrillo J.,Complutense University of Madrid | Benavides J.,Institute Ganaderia Of Montana Csic Ule | Alvarez-Garcia G.,Complutense University of Madrid | Fuertes M.,Institute Ganaderia Of Montana Csic Ule | Ortega-Mora L.M.,Complutense University of Madrid
Veterinary Research | Year: 2014

Neospora caninum has been detected only sporadically in cases of ovine abortion, and it has therefore traditionally been considered as an unimportant parasite in small ruminants. This study was carried out with the aim of identifying the pathogen causing serious reproductive problems on a commercial sheep farm. Sera from all rams and ewes tested negative for antibodies against Border disease virus, Schmallenberg virus and Coxiella burnetii, and infections by these agents were therefore ruled out. Nevertheless, seropositivity to N. caninum and/or Toxoplasma gondii was detected, although the seroprevalence was higher in the case of N. caninum. The percentage of lambings and the number of lambs per dam were significantly lower in ewes that were seropositive to N. caninum while no effect on these parameters was detected in ewes that were seropositive to T. gondii. There was also no evidence of infection by T. gondii in the foetal/lamb tissues analyzed by PCR and/or immunohistopathological techniques. On the contrary, the DNA of N. caninum was detected in 13 out of 14 foetuses/lambs descendant from dams seropositive to this parasite. Characteristic lesions caused by N. caninum and/or its antigen were also detected. Genotyping of the N. caninum DNA revealed only two closely related microsatellite multilocus genotypes. The results clearly demonstrate that infection by N. caninum was the cause of the low reproductive performance of this sheep flock. © 2014 González-Warleta et al.; licensee BioMed Central Ltd.


Gomez-Cortes P.,CSIC - Institute of Refrigeration | Toral P.G.,Institute Ganaderia Of Montana Csic Ule | Frutos P.,Institute Ganaderia Of Montana Csic Ule | Juarez M.,CSIC - Institute of Refrigeration | And 2 more authors.
Food Chemistry | Year: 2011

The aim of this study was to investigate a suitable amount of sunflower oil (SO) inclusion in dairy sheep diet, in order to enhance the milk content of some potentially healthy fatty acids (FA; such as cis-9 trans-11 C18:2 -RA- and trans-11 C18:1 -VA-) without increasing other potentially unhealthy FA (such as trans-10 C18:1) or detrimentally affecting animal performance. Eighty dairy ewes were allocated to 4 treatments: no lipid supplementation (control), supplementation with 17 (SO1), 34 (SO2), or 51 (SO3) g of SO per kg of dry matter, for 28. days. Incremental amounts of dietary SO did not affect milk production nor the milk's fat, protein and lactose contents. However, the FA profile was substantially modified. Treatment SO3 caused the highest enrichment in VA and RA and decreases in saturated FA, but it also enhanced the accumulation of trans-10 C18:1, which might jeopardise potentially the health-promoting properties of the ewe milk fat. © 2010 Elsevier Ltd.


Toral P.G.,Institute Ganaderia Of Montana Csic Ule | Frutos P.,Institute Ganaderia Of Montana Csic Ule | Hervas G.,Institute Ganaderia Of Montana Csic Ule | Gomez-Cortes P.,CSIC - Institute of Refrigeration | And 2 more authors.
Journal of Dairy Science | Year: 2010

Ruminant diet supplementation with sunflower oil (SO) and fish oil (FO) has been reported as a good strategy for enhancing some milk fat compounds such as conjugated linoleic acid (CLA) and n-3 polyunsaturated fatty acids in dairy cows, but no information is available regarding dairy sheep. In this work, ewe diet was supplemented with FO, alone or in combination with SO, with the aim of improving milk nutritional value and evaluating its effect on animal performance. Sixty-four Assaf ewes in mid lactation, fed a high-concentrate diet, were distributed in 8 lots of 8 animals each and assigned to 4 treatments (2 lots/treatment): no lipid supplementation (control) or supplementation with 20 g of SO/kg (SO), 10 g of FO/kg (FO), or 20 g of SO plus 10 g of FO/kg (SOFO). Milk production and composition, including a complete fatty acid profile, were analyzed on d 0, 3, 7, 14, 21, and 28 of treatments. Supplementation with FO tended to reduce dry matter intake compared with the control treatment (-15%), and its use in combination with SO (SOFO) resulted in a significant decrease in milk yield as well (-13%). All lipid supplements reduced milk protein content, and FO also reduced milk fat content by up to 21% alone (FO) and 27% in combination with SO (SOFO). Although the mechanisms involved in FO-induced milk fat depression are not yet well established, the observed increase in some milk trans-FA that are putative inhibitors of milk fat synthesis, such as trans-9,. cis-11 CLA, and the 63% decrease in C18:0 (consistent with the theory of reduced milk fat fluidity) may be involved. When compared with the control, lipid supplementation remarkably improved the milk content of rumenic acid (cis-9,. trans-11 CLA; up to 4-fold increases with SO and SOFO diets), whereas FO-containing diets also increased milk n-3 polyunsaturated fatty acids, mainly docosahexaenoic acid (with mean contents of 0.29 and 0.38% of total fatty acids for SOFO and FO, respectively), and reduced the n-6:n-3 FA ratio to approximately half the control value. All lipid supplements resulted in high levels of some trans-FA, mainly trans-11 C18:1 (vaccenic acid) but also trans-10 C18:1. © 2010 American Dairy Science Association.


Toral P.G.,Institute Ganaderia Of Montana Csic Ule | Hervas G.,Institute Ganaderia Of Montana Csic Ule | Gomez-Cortes P.,CSIC - Institute of Refrigeration | Frutos P.,Institute Ganaderia Of Montana Csic Ule | And 2 more authors.
Journal of Dairy Science | Year: 2010

In an attempt to develop strategies for enhancing the nutritional value of sheep milk fat, dairy ewe diet was supplemented with 3 incremental levels of marine algae (MA), in combination with sunflower oil, to evaluate the effects of these marine lipids on milk fatty acid (FA) profile and animal performance. Fifty Assaf ewes in mid lactation were distributed in 10 lots of 5 animals each and allocated to 5 treatments (2 lots per treatment): no lipid supplementation (control) or supplementation with 25g of sunflower oil/kg of DM plus 0 (SO), 8 (SOMA1), 16 (SOMA2), or 24 (SOMA3) g of MA (56.7% ether extract)/kg of DM. Milk production and composition, including FA profile, were analyzed on d 0, 3, 7, 14, 21, and 28 of treatment. Neither intake nor milk yield were significantly affected by lipid addition, but all MA supplements decreased milk fat content from d 14 onward, reaching a 30% reduction after 28 d on SOMA3. This milk fat depression might be related not only to the joint action of some putative fat synthesis inhibitors, such as trans-9,cis-11 C18:2 and probably trans-10 C18:1, but also to the limited ability of the mammary gland to maintain a desirable milk fat fluidity, that would have been caused by the noticeable increase in trans-C18:1 together with the lowered availability of stearic acid for oleic acid synthesis through Δ9-desaturase. Furthermore, all lipid supplements, and mainly MA, reduced the secretion of de novo FA (C6:0-C14:0) without increasing the yield of preformed FA (>C16). Supplementation with sunflower oil plus MA resulted in larger increases in cis-9,trans-11 C18:2 than those observed with sunflower oil alone, achieving a mean content as high as 3.22% of total FA and representing a more than 7-fold increase compared with the control. Vaccenic acid (trans-11 C18:1) was also significantly enhanced (on average +794% in SOMA treatments), as was C22:6 n-3 (DHA) content, although the transfer efficiency of the latter, from the diets to the milk, was very low (5%). However, the highest levels of MA inclusion (SOMA2 and SOMA3) reduced the milk n-6:n-3 ratio, but MA supplements caused an important increase in trans-10 C18:1, which would rule out the possibility that this milk has a healthier fat profile before determining the specific role of each individual FA and ensuring that this trans-FA is at least innocuous in relation to cardiovascular disease risk. © 2010 American Dairy Science Association.


Bodas R.,Institute Ganaderia Of Montana Csic Ule | Manso T.,University of Valladolid | Mantecon A.R.,Institute Ganaderia Of Montana Csic Ule | Juarez M.,CSIC - Institute of Refrigeration | And 2 more authors.
Journal of Agricultural and Food Chemistry | Year: 2010

The purpose of this work was to obtain a cheese from ewes milk with a healthier fatty acid (FA) profile. To achieve our aim, 48 ewes (12 per treatment) were fed diets supplemented with 3% of plant oils: palm (used as control), olive (OO), soybean (SO), and linseed (LO). Milk samples from each treatment were collected to manufacture cheeses. The cheesemaking process did not modify the dairy fat FA profile, but OO, SO, and LO did reduce the C12:0 + C14:0 + C16:0 content in dairy fat, thus decreasing the atherogenic index value in the cheeses. Percentages of cis-9 trans-11 C18:2 in cheeses ranged from the 0.43 control value to 0.92, 1.64, and 2.71 with OO, LO, and SO respectively, following the same pattern as trans-11 C18:1. In contrast, trans-10 C18:1 levels were always below 1%. The lowest n-6/n-3 ratio obtained with LO (1.43) suggests that such lipid supplementation would be the most effective nutritional strategy for improving cheese FA profiles. © 2010 American Chemical Society.


Toral P.G.,Institute Ganaderia Of Montana Csic Ule | Hervas G.,Institute Ganaderia Of Montana Csic Ule | Belenguer A.,Institute Ganaderia Of Montana Csic Ule | Bichi E.,Institute Ganaderia Of Montana Csic Ule | Frutos P.,Institute Ganaderia Of Montana Csic Ule
Journal of Dairy Science | Year: 2013

Despite controversy surrounding the ability of tannins to modulate the fatty acid (FA) profile of ruminant-derived products, reports on this issue are still very limited for dairy sheep. This study was conducted to examine the effect of the inclusion of quebracho tannins in a diet rich in linoleic acid on ewe performance and milk FA composition. Thirty-six lactating ewes were distributed into 6 lots and allocated to 2 treatments (3 lots/treatment): control or quebracho. All sheep received a total mixed ration based on alfalfa hay and a concentrate (forage:concentrate ratio of 40:60) supplemented with 20. g of sunflower oil/kg of dry matter plus 0 (control diet) or 20. g of an extract of quebracho tannins/kg of dry matter (QUE diet). Milk production and composition were analyzed on d 0, 3, 6, 9, 12, 15, 18, 21, 24, and 27 on treatments, and milk FA profile on d 0, 3, 6, 12, 18, and 27. On d 27, samples of rumen fluid were collected for pH, and lactate, ammonia, and volatile FA concentration analysis. Feeding the QUE diet had no apparent effect on animal performance and hardly modified ruminal fermentation characteristics, except for a reduction in the molar proportions of minor volatile FA. Dietary tannins increased the milk concentration of several 18:1 and 18:2 isomers and decreased that of branched-chain FA. Some of these changes were relatively constant throughout the experiment (e.g., cis-12 18:1 and trans-9,. cis-12 18:2), whereas others varied over time (e.g., trans-10 18:1, which increased gradually with the QUE diet). Significant differences between treatments in trans-11 18:1 and cis-9,. trans-11 conjugated linoleic acid were only observed on d 3. Overall, addition of quebracho tannins to a diet rich in linoleic acid did not prove useful to beneficially modify milk FA composition, especially over the long term. © 2013 American Dairy Science Association.


Carreno D.,Institute Ganaderia Of Montana Csic Ule | Hervas G.,Institute Ganaderia Of Montana Csic Ule | Toral P.G.,Institute Ganaderia Of Montana Csic Ule | Belenguer A.,Institute Ganaderia Of Montana Csic Ule | Frutos P.,Institute Ganaderia Of Montana Csic Ule
Animal Feed Science and Technology | Year: 2015

The ability of tannins to interfere with ruminal biohydrogenation (BH) and modulate the fatty acid (FA) profile of ruminant-derived products is highly controversial, which is probably related to the type of tannin and the dosage rate. Therefore, this in vitro study was conducted to analyse the effect of four commercial extracts of tannins (from chestnut, oak, quebracho and grape) at four doses (20, 40, 60 and 80. g/kg diet DM) with the aim of selecting an effective treatment to modulate the BH of unsaturated FA. Two in vitro assays with batch cultures of rumen microorganisms, using cannulated ewes as donors of rumen inocula, were performed. The incubated substrate (a total mixed ration similar to that fed to the animals) was supplemented with 20. g of sunflower oil/kg DM. The first experiment followed a 4. ×. 4. +. 1 design (i.e., 4 types of tannins. ×. 4 doses of each one, and a control), and treatment effects on the FA composition of the ruminal digesta were examined by gas chromatography. On the basis of these results, the second experiment was conducted to make sure that the selected dose and type of tannin would not impair rumen fermentation. To this end, gas production kinetic parameters, extent of degradation, in vitro true substrate digestibility, pH, and ammonia and volatile FA concentrations, as well as the bacterial community (by terminal restriction fragment length polymorphism, T-RFLP) were examined. All tannin extracts were able to modulate the in vitro BH of unsaturated FA. However, the high dose required in many cases suggests that their efficacy would be rather limited in terms of animal feeding. On the other hand, the oak tannin extract, at a dose of 20. g/kg diet DM, increased total polyunsaturated FA, 18:3n-3, 18:2n-6 and trans-11 18:1, and decreased trans-10 18:1 and 18:0 rumen concentrations without eliciting any negative response in ruminal fermentation. Although this treatment had no discernible effects on the bacterial community structure and diversity, a few fragments compatible with uncultured Lachnospiraceae species were affected. © 2015 Elsevier B.V.


Vieira C.,Leon Institute of Technology | Fernandez-Diez A.,University of León | Mateo J.,University of León | Bodas R.,Institute Ganaderia Of Montana Csic Ule | And 2 more authors.
Meat Science | Year: 2012

The effect of different vegetable oils used in the diet of lactating ewes on the meat quality of their suckling lambs has been evaluated. Lambs (males and females) were slaughtered at 11. kg. Fortyeight lactating Churra ewes (prolificacy 1.5) and their suckling lambs were assigned to four treatments according to the oil added (3% on weight basis) to the ewes' daily ration: palm oil as control (CON); olive oil (OLI); soybean oil (SOY); and linseed oil (LIN). Analyses of pH, colour, thiobarbituric acid reactive substances (TBARS), tocopherol levels, volatile compounds and a sensory evaluation were carried out on suckling lambs' meat. Results showed no substantial effect on pH, colour, TBARS and tocopherol levels. Volatiles typically derived from lipid oxidation were higher in SOY group. However, panellists were only able to correctly identify samples from LIN group. Furthermore, the meat from LIN group showed lower scores towards odour and flavour quality and overall liking than that from the rest of treatments. © 2012 Elsevier Ltd.

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