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Hannover, Germany

Hertzsch R.,University of Leipzig | Emmerich I.U.,University of Leipzig | Lachenmeier D.W.,Chemisches und Veterinaruntersuchungsamt CVUA | Sproll C.,Chemisches und Veterinaruntersuchungsamt CVUA | And 3 more authors.
Tierarztliche Praxis Ausgabe G: Grosstiere - Nutztiere | Year: 2015

Opioid alkaloids were identified in the urine of horses during an anti-doping control and in a case of intoxication. In both cases, it was suspected that the horses had ingested poppy-contaminated feed. To verify this suspicion, possible opioid alkaloid sources in Germany were identified through a literature research. Additionally, the contaminated feed was botanically and chemically analysed. The results indicated that both cases were most probably caused by the poppy in the feed. This highlights the previously underestimated risk of an intake of poppy-contaminated feed in horses. Recommendations are formulated for the prevention of positive doping-test results and intoxications by poppy-contaminated feeds in horses. Furthermore, a threshold for morphine in urine samples in competing horses is proposed. © Schattauer 2015.

Hildebrand B.,University of Hohenheim | Boguhn J.,University of Hohenheim | Danicke S.,Institute For Tierernahrung | Rodehutscord M.,University of Hohenheim
Journal of Animal Physiology and Animal Nutrition | Year: 2012

In this study, the effect of Fusarium toxin-contaminated triticale (FUS) at high (60%) and low (30%) concentrate proportion in ruminant rations on ruminal fermentation, microbial protein synthesis and digestibility was investigated, using in vivo and in vitro methods. Significant effects of the forage-to-concentrate ratio on ruminal degradation and digestibility of crude nutrients and detergent fibre fractions as well as on the pH value and the concentration of short chain fatty acids (SCFA) in rumen fluid were found. The production of SCFA was affected, and the degradation of crude fibre and neutral detergent fibre in the rumen was reduced by the inclusion of FUS at high concentrate proportion. The efficiency of microbial crude protein synthesis was higher in diets with 60% than in diets with 30% concentrates, but was impaired in the presence of FUS in vitro at the high concentrate level. Marginal effects of FUS on the amino acid pattern of microbial protein were detected. It was concluded that the use of FUS in high concentrate diets can influence ruminal fermentation and microbial protein synthesis at a dietary deoxynivalenol concentration below 5mg/kg dry matter. © 2011 Blackwell Verlag GmbH.

Visscher C.,Institute For Tierernahrung
Praktische Tierarzt | Year: 2015

In light of the efforts in pig farming, to further reduce the use of antibiotic agents, also approaches in the field of animal nutrition will be discussed as a possible part of the solution. With the use of probiotics certain hopes are linked. Today probiotics are ascribed features such as competition with pathogenic microorganisms for nutrients and binding sites on the epithelium, the production of various antimicrobial components against pathogenic microorganisms, as well as interfering with the immune system in the gastrointestinal tract. Especially in swine, the phase of weaning of piglets from the sows is of interest. Here the piglets are particularly susceptible to diarrheal diseases of various origins. The immaturity of the intestinal immune system and the elimination of with breast milk ingested immunoglobulin A and other bioactive substances explain the susceptibility of such diseases. Although previous studies on the effects of probiotic bacteria in pigs often show positive effects on the performance and health, the positive experiences are not consistent. In view of the reduction deemed necessary concerning the use of antibiotics in food-producing animals, approaches for the targeted use of probiotic bacteria will be of interest.

Wolf P.,Institute For Tierernahrung | Siesenop U.,Institute For Mikrobiologie | Verspohl J.,Institute For Mikrobiologie | Kamphues J.,Institute For Tierernahrung
Tierarztliche Praxis Ausgabe K: Kleintiere - Heimtiere | Year: 2014

Objective: Evaluation of the hygienic status of feedstuffs for small mammals, including rabbits, guinea pigs and chinchilla, kept as pets. Material and methods: A total of 356 feedstuffs that had been sent to the consulting service of the institute with a usable case history between January 2000 and April 2011 were analysed by sensory testing and microbiological examination. The interpretation of the micro - bio logical results was performed with regard to benchmarks given by the working group "feed microbiology of section VI of the VDLUFA" for hay, straw and further feedstuffs. Results: Within the sensory evaluation, the parameters "texture" (indicating the moisture content of a feed) and "smell" (mouldy or yeasty nuances) provided the first important information on feed quality. Deviations in these parameters were observed in 29.0% and 41.2%, respectively, of the analysed roughages and in 27.4% and 15.1% of mixed feeds, respectively. In about 10% of the feeds, pests (mites, Psocoptera as an indicator of mould infestation) were diagnosed microscopically. Using the differentiation of the germs as described above, faults in the hygienic quality could be detected mainly in roughage (hay, straw) and the so-called coloured feed (mixed feed based on ingredients including oat, barley or corn) whereas they occurred rarely in commercial pel - leted mixed feeds based on green meal. Conclusion and clinical rele vance: In cases of hygienic deficits in feedstuffs, it was often unclear whether the reduced hygienic quality resulted from the pro - duction conditions (i.e. in the factory) or by improper storage of the feedstuffs by traders or pet owners. A general recommendation should be given to the owner to check the feed using a critical sensory eva - luation to avoid health disorders in pets due to higher levels of germs or poisonous plants. © Schattauer 2014.

Boguhn J.,University of Hohenheim | Neumann D.,Johann Heinrich Von Thunen Institute | Helm A.,Martin Luther University of Halle Wittenberg | Strobel E.,Institute For Tierernahrung | And 3 more authors.
Archives of Animal Nutrition | Year: 2010

The objective of this study was to investigate the effects of the concentrate proportion and Fusarium toxin-contaminated triticale (FCT) in the diet on nutrient degradation, microbial protein synthesis and structure of the microbial community, utilising a rumen simulation technique and single-strand conformation polymorphism (SSCP) profiles based on PCR-amplified small subunit ribosomal RNA genes. Four diets containing 60% or 30% concentrates on a dry matter basis with or without FCT were incubated. The fermentation of nutrients and microbial protein synthesis was measured. On the last day of incubation, microbial mass was obtained from the vessel liquid, DNA was extracted and PCR-primers targeting archaea, fibrobacter, clostridia, bifidobacteria, bacillii, fungi, and bacteria were applied to separately study the individual taxonomic groups with SSCP. The concentrate proportion affected the fermentation and the microbial community, but not the efficiency of microbial protein synthesis. Neither the fermentation of organic matter nor the synthesis and composition of microbial protein was affected by FCT. The fermentation of detergent fibre fractions was lower in diets containing FCT compared to diets with uncontaminated triticale. Except for the clostridia group, none of the microbial groups were affected by presence of FCT. In conclusion, our results give no indication that the supplementation of FCT up to a deoxynivalenol concentration in the diet of 5 mg per kg dry matter affects the fermentation of organic matter and microbial protein synthesis. These findings are independent of the concentrate level in the diets. A change in the microbial community composition of the genus Clostridia may be the reason for a reduction in the cellulolytic activity. © 2010 Taylor & Francis.

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