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Schulze M.,Institute for the Reproduction of Farm Animals Schonow | Ammon C.,Leibniz Institute for Agricultural Engineering | Nurnberg G.,Leibniz Institute for Farm Animal Biology | Rudiger K.,Institute for the Reproduction of Farm Animals Schonow | And 2 more authors.
Veterinary Journal | Year: 2016

The current study reports the parasitological results of a quality control audit in 21 European boar studs. Field investigations were performed over a 2-year period (2012-2013) during the winter and spring. From each stud, an average of 30 (range, 25-33) individual faecal samples and ejaculates from 615 randomly selected Pietrain boars were analysed. Statistical analysis revealed a significant effect (P < 0.0001) of deworming regimen (DR) × age class of boar (A) and housing condition (H) × A on the presence of parasites. A second model indicated a significant effect (P = 0.0262) of DR × H × A on the presence of parasites. Sperm output was significantly affected (P < 0.0001) by the DR. Based on this study, recommendations for deworming AI boars are proposed. © 2015 Elsevier Ltd. Source


Gartner M.A.,Free University of Berlin | Bondzio A.,Free University of Berlin | Braun N.,Institute for the Reproduction of Farm Animals Schonow | Jung M.,Institute for the Reproduction of Farm Animals Schonow | And 2 more authors.
PLoS ONE | Year: 2015

Bacterial infections and inflammation of the uterus are common in dairy cattle after parturition. In particular, pathogenic bacteria that cause endometritis have been the focus of research in cattle reproduction in the last ten years. The aim of the present study was to identify commensal lactobacilli in the bovine uterus and to examine their influence on the synthesis of pro-inflammatory factors in bovine endometrial epithelial cells in vitro. Lactobacillus species were isolated from healthy bovine uteri and further characterised. Bovine endometrial epithelial cells in the second passage (n = 5 animals) were co-cultured with the autochthonous isolates L. buchneri, L. ruminis and L. amylovorus as well as with a commercially available L. vaginalis in different multiplicities of infection (MOI = 1, 5 and 10, respectively). Endometrial epithelial cells cultured without bacteria served as controls. At distinct points in time (2, 4 and 6 h) total RNA was extracted from co-cultured epithelial cells and subjected to reverse transcription quantitative PCR of pro-inflammatory factors. Furthermore, the release of such factors by co-cultured epithelial cells was measured by ELISA or EIA after 24 and 48 h. L. ruminis and L. amylovorus induced increased interleukin (IL) IL1A, IL6, IL8 and prostaglandin-endoperoxide synthase 2 mRNA levels and the release of IL8 and prostaglandin F2α in endometrial epithelial cells compared with control cells. In contrast, L. buchneri did not significantly influence the expression and release of these factors. Tolllike receptors 2 and 6 transcripts were found unchanged in co-cultured and untreated epithelial cells in vitro. However, endometrial epithelial cells of each animal showed individual differences in the response to bacterial load. These results suggest that Lactobacillus species are present in the bovine uterus, revealing immunomodulatory properties. © 2015 Gärtner et al. Source

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