Institute for the Application of Nuclear Energy

Banatska, Serbia

Institute for the Application of Nuclear Energy

Banatska, Serbia
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Matko Stamenkovic U.,University of Belgrade | Andrejic G.,University of Belgrade | Andrejic G.,Institute for the Application of Nuclear Energy | Mihailovic N.,Institute for the Application of Nuclear Energy | Sinzar-Sekulic J.,University of Belgrade
Applied Ecology and Environmental Research | Year: 2017

We investigate the nickel concentration in serpentine soil samples from several areas in central region of Bosnia and Herzegovina. Concentration of some other elements such as P, K, Ca, Mg, Fe, Mn, Pb, Co, and Cd were determined as well. Selected soil samples were typical of ultramafic sites with low concentrations of P, K and Ca and high concentrations of Mg, Fe, Ni and Zn. The Mg/Ca ratio was relatively high (0.78–30.61). The levels of P, K, Fe and Zn were high, Mn and Cu occurred in low amounts, whereas, Cd, Co and Pb were only traceable. Principal component analysis pointed out the correlation of elements originating from ultramafic bedrock and their possible phytoextraction. The concentration of Ni in tissues of all investigated plant samples was high to extremely high. High concentrations of Co in plant tissues were recorded also. Bearing in mind that A. murale at investigated sites in Bosnia and Herzegovina showed a high capacity for accumulation and translocation of Ni and generally, shared high tolerance to other metals, this plant may be used to clean up soils contaminated with heavy metals, as well as for phytomining. © 2017, ALÖKI Kft., Budapest, Hungary.

Penezic A.,Institute for the Application of Nuclear Energy | Miljus G.,Institute for the Application of Nuclear Energy | Milutinovic B.,Institute for the Application of Nuclear Energy | Nedic O.,Institute for the Application of Nuclear Energy
Clinica Chimica Acta | Year: 2017

A microscale procedure for the isolation of transferrin directly from human serum (hTf) is described in this study. The protocol is based on three precipitation steps without application of chromatography. It lasts 90 min with the initial sample volume of 250 μL. The yield of the isolated hTf is 58%, which is considerable in biochemical terms. The purity of the isolated hTf is 97%, as assessed by three methods: electrophoresis followed by protein staining, immunoblotting and HPLC. Immunoblotting with antibodies against other major serum proteins indicated that isolated hTf does not contain albumin, immunoglobulin G or alpha-2-macroglobulin. Lectin dot-blot demonstrated that isolated hTf preserved its glycan moieties. Fluorescent emission spectroscopy of the isolated hTf has shown no changes in tertiary structure. Isolated hTf was approximately 26% saturated with iron ion, which is comparable to physiological value (although a degree of saturation decreases to some extent during isolation procedure). Finally, co-immunoprecipitation experiment confirmed that isolated hTf retained its ligand characteristics crucial for the ligand-receptor type of interaction with the hTf receptor. To conclude, the procedure described in this work, is time and cost-effective, allows multiple sample handling and provides high-purity hTf isolate with preserved structural and functional properties. © 2017 Elsevier B.V.

Momcilovic M.,Institute for the Application of Nuclear Energy | Kovacevic J.,Geological Institute of Serbia | Dragovic S.,Institute for the Application of Nuclear Energy
Radiation Measurements | Year: 2010

A uranium mineralized area of Stara Planina Mt., Serbia, where uranium ore was exploited for seven years was characterized radiologically. Results were compared with those for an area of background radiation in the northern part of the mountain. The terrestrial gamma dose rate due to 238U, 232Th and 40K in the area affected by mining activities was twofold higher than that of background area. The radiological situation of the affected area is not of immediate concern, except one location with elevated external hazard index where remediative measures taking into account site-specific ecological characteristics should be planned and implemented. © 2010 Elsevier Ltd. All rights reserved.

Durovic S.,University of Belgrade | Jakovljevic K.,University of Belgrade | Buzurovic U.,Natural History Museum | Niketic M.,Natural History Museum | And 2 more authors.
Australian Journal of Botany | Year: 2016

The aim of the present study was to compare trace element profiles and the differences in uptake and translocation of trace elements in plants from five populations (three from Greece and two from Serbia) belonging to three subspecies of Silene parnassica Boiss. & Sprun. growing on ophiolitic substrates. For comparison of the subspecies, bioconcentration and translocation factors were used, as well as Spearman's rank correlation coefficients for concentrations of elements in root and shoot samples. Chemical characteristics of the soil samples (pH, organic C, P2O5, K2O, Ca, Fe, Mg, Mn, Ni, Zn, Cu, Cr, Co, Cd and Pb) and plant samples (P2O5, K2O, Ca, Fe, Mg, Mn, Ni, Zn, Cu, Cr, Co, Cd and Pb) were determined, as well as bioconcentration and translocation factors and correlation matrices. All the three subspecies acted as strong Ni accumulators, with equal concentrations of Ni in roots and shoots, the values being several times higher than 100mgkg-1, exceeding concentrations of available Ni in the soil. Concentrations of Cu and Cr in the aboveground plant tissues in samples from three localities were several times higher than expected, even for plants growing on metalliferous soils, exceeding, by multiple times, the available concentrations in the soil samples. © CSIRO 2016.

Mihajlovski K.R.,University of Belgrade | Carevic M.B.,University of Belgrade | Devic M.L.,Institute for the Application of Nuclear Energy | Siler-Marinkovic S.,University of Belgrade | And 2 more authors.
International Biodeterioration and Biodegradation | Year: 2015

A novel strain of Paenibacillus chitinolyticus CKS1 was isolated from forest soil and identified as a potent cellulase producer. The strain was able to grow on various commercial substrates including microcrystalline cellulose (Avicel), carboxymethylcellulose (CMC), and cellobiose but also on lignocellulosic waste material such as medicinal herbs waste and sawdust. On all these substrates the strain produced cellulase composed of two subunits (~70 and ~45 kDa) that was active on CMC, Avicel and filter paper. The maximal Avicelase activity (1.94 U/ml) was reached in a medium that contained 0.1% (w/v) of medicinal herbs waste, 3 g l-1 of yeast extract and 5.0 g l-1 of casein hydrolysate in 0.1 M phosphate buffer pH 7, after 48 h of incubation at 30 °C. The Avicelase performed optimally at 80 °C and at pH 4.8. Addition of K+ increased the Avicelase activity almost three fold and the enzyme retained 48.39% of the initial activity after 60 min. The product of Avicel and CMC hydrolysis was glucose with traces of other soluble sugars, indicating that the crude cellulase produced on waste material using the novel P. chitinolyticus strain CKS 1 could be used in eco-friendly processes of cellulose bioconversion, such as enzymatic saccharification of lignocellulosic materials in processes performed under acidophilic conditions and high temperatures. © 2015 Elsevier Ltd.

Radovic I.,Institute for the Application of Nuclear Energy | Gruden-Movsesijan A.,Institute for the Application of Nuclear Energy | Ilic N.,Institute for the Application of Nuclear Energy | Mostarica-Stojkovic M.,University of Belgrade | Sofronic-Milosavljevic L.,Institute for the Application of Nuclear Energy
Memorias do Instituto Oswaldo Cruz | Year: 2012

Like other helminths, Trichinella spiralis has evolved strategies to allow it to survive in the host organism, including the expression of epitopes similar to those present in either expressed or hidden host antigens. To identify T. spiralis-derived antigens that are evolutionarily conserved in the parasite and its host and that could be responsible for its evasion of the host immune response, we examined the reactivity of six different types of autoantibodies to T. spiralis larvae from muscle. T. spiralis antigens that share epitopes with human autoantigens were identified by assessing the cross-reactivity of autoantibody-containing serum samples with T. spiralis antigens in the absence of specific anti-parasite antibodies. Of the 55 autoantibody-containing human serum samples that we analysed by immunohistological screening, 24 (43.6%) recognised T. spiralis muscle larvae structures such as the subcuticular region, the genital primordium or the midgut. Using Western blots, we demonstrated that the same sera reacted with 24 protein components of T. spiralis muscle larvae excretory-secretory L1 antigens. We found that the human autoantibodies predominantly bound antigens belonging to the TSL1 group; more specifically, the autoantibody-containing sera reacted most frequently with the 53-kDa component. Thus, this protein is a good candidate for further studies of the mechanisms of T. spiralis-mediated immunomodulation.

Jankovic M.,University of Belgrade | Jankovic M.,Institute for the Application of Nuclear Energy
Journal of Medical Biochemistry | Year: 2011

Protein glycosylation is a ubiquitous and complex co- and post-translational modification leading to glycan formation, i.e. oligosaccharide chains covalently attached to peptide backbones. The significance of changes in glycosylation for the beginning, progress and outcome of different human diseases is widely recognized. Thus, glycans are considered as unique structures to diagnose, predict susceptibility to and monitor the progression of disease. In the «omics» era, the glycome, a glycan analogue of the proteome and genome, holds considerable promise as a source of new biomarkers. In the design of a strategy for biomarker discovery, new principles and platforms for the analysis of relatively small amounts of numerous glycoproteins are needed. Emerging glycomics technologies comprising different types of mass spectrometry and affinity-based arrays are next in line to deliver new analytical procedures in the field of biomarkers. Screening different types of glycomolecules, selection of differentially expressed components, their enrichment and purification or identification are the most challenging parts of experimental and clinical glycoproteomics. This requires large-scale technologies enabling high sensitivity, proper standardization and validation of the methods to be used. Further progress in the field of applied glycoscience requires an integrated systematic approach in order to explore properly all opportunities for disease diagnosis.

Sosic-Jurjevic B.,University of Belgrade | Filipovic B.,University of Belgrade | Ajdzanovic V.,University of Belgrade | Savin S.,Institute for the Application of Nuclear Energy | And 3 more authors.
Experimental Biology and Medicine | Year: 2010

High intake of soybean phytoestrogens, isoflavones genistein (G) and daidzein (D), has been associated with health benefits. However, isoflavones were reported to affect adversely thyroid function in the presence of other goitrogenic factors. As the thyroid gland becomes functionally impaired with age, we examined whether supplementary doses of G or D would affect morphology and function of pituitary-thyroid axis in middle-aged male rats. Sixteen-month-old orchidectomized Wistar rats were treated with 10 mg/kg of either G or D, while the control sham-operated and orchidectomized group received just the vehicle for three weeks. The animals were fed soy-free diet with increased iodine content, and killed 24 h after the last treatment. Their pituitaries and thyroids were excised and prepared for further immunohistochemical and morphometric investigation. The concentrations of thyroid-stimulating hormone (TSH), total T4 and T3, in the serum were determined. In both isoflavone-treated groups, pituitary TSH-immunopositive cells had increased cellular volume and relative volume density (P< 0.05), as well as increased serum TSH levels (P< 0.05) in comparison to the controls; their thyroid tissue was characterized by increased volume of thyroglobulin-immunopositive epithelium (P< 0.05), epithelial height and index of activation rate (P< 0.05), while the volume of luminal colloid, and total serum T4 and T3 levels decreased (P< 0.05) in comparison to the controls. In conclusion, this study provides the first direct evidence that both G and D can induce microfollicular changes in the thyroid tissue and reduce the level of thyroid hormones in Orx middle-aged male rats, a model of andropause. This reduction consequently led to a feedback stimulation of pituitary TSH cells. The detected stimulatory effect was higher in the daidzein-treated rats. Copyright © 2010 by the Society for Experimental Biology and Medicine.

PubMed | University of Belgrade, Institute for the application of nuclear energy and Center for Endocrine Surgery
Type: | Journal: Diagnostic pathology | Year: 2015

Nodular follicular lesions of thyroid gland comprise benign and malignant neoplasms, as well as some forms of hyperplasia. Follicular refers to origin of cells and in the same time to growth pattern - building follicles. Nodular follicular thyroid lesions have in common many morphological features, therefore attempts were made to define additional criteria for distinction between follicular adenoma, follicular carcinoma and follicular variant of papillary carcinoma. Increasing number of immunohistochemical markers is in the continual process of evaluation.Tissue microarrays incorporating, total 201 cases, out of which 122 malignant and 79 benign follicular lesions, including neoplastic and non-neoplastic, were constructed and immunostained with antibodies to CD56, CK19, Galectin-3, HBME-1. Tissue cores were exclusively being acquired from tumour/lesion on interface with normal thyroid tissue. A systematic review of literature was done for period from the year 2001 to present time.All analysed markers may make a difference between benign lesions/tumours from differentiated thyroid carcinomas (p=<0.01, for all markers). Expression of all markers is significantly higher in papillary carcinoma than in follicular adenoma (p<0.01). Statistically significant difference in expression of Galectin-3 and CD56 between follicular carcinoma and follicular adenoma was registered (p=0.043; p=0.028, respectively). The only marker which expression showed statistically significant difference between adenoma and carcinoma of Hurthle cells was Galectin 3 (p=0.041). CK19 and HBME-1 were significantly expressed more in papillary carcinoma as compared to follicular carcinoma.Galectin 3 is most sensitive marker for malignancy, while loss of expression of CD56 is very specific for malignancy. Expected co-expression for combination of markers in diagnosis of follicular lesions decreases sensitivity and increases specificity for malignancy.

Dragovic S.,Institute for the Application of Nuclear Energy | Howard B.J.,UK Center for Ecology and Hydrology | Caborn J.A.,UK Center for Ecology and Hydrology | Barnett C.L.,UK Center for Ecology and Hydrology | Mihailovic N.,Institute for the Application of Nuclear Energy
Environmental Monitoring and Assessment | Year: 2010

Few data are available to quantify the transfer of both natural and anthropogenic radionuclides to detritivorous invertebrates to facilitate estimation of the internal dose to such biota in models used to assess radiation exposure. To enhance the available data, activity concentrations of 137Cs, 40K, 90Sr, 239+240Pu, 241Am, 235U and 238U were measured in ants (Formicidae) and corresponding undisturbed soil collected from the Zlatibor mountain in Serbia and ant/soil concentration ratios (CR) calculated. The 241Am concentration ratios for ants were fourfold higher than those calculated for ants in a previous study whereas they are similar to the more numerous data previously reported for a range of detritivorous invertebrates in other studies. CR values for 137Cs in ants were similar to the few other reported values and slightly lower than those for a range of detritivorous invertebrates. Those for 239+240Pu were slightly higher than those for ants in two other studies but they were close to upper limit of a range of data reported for detritivorous invertebrates. All the CR values will be included in a future revision of the ERICA Tool database and will particularly improve the information available for uranium. © 2009 Springer Science+Business Media B.V.

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