Institute for Small Animal Research and Co ordination Center for Gene Conservation

Gödöllő, Hungary

Institute for Small Animal Research and Co ordination Center for Gene Conservation

Gödöllő, Hungary
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Liptoi K.,Institute for Small Animal Research and Co ordination Center for Gene Conservation | Horvath G.,Institute for Small Animal Research and Co ordination Center for Gene Conservation | Gal J.,Szent Istvan University | Varadi E.,Institute for Small Animal Research and Co ordination Center for Gene Conservation | Barna J.,Institute for Small Animal Research and Co ordination Center for Gene Conservation
Animal Reproduction Science | Year: 2013

The aim of the study was to devise a technique to allow transfer of ovarian and testicular tissues obtained from one day old chicks to recipient animals. The following combinations of chicken breeds were used: Tetra SL/Tetra SL, Tetra SL/Harco, Tetra SL/Black Transylvanian Naked Neck, Godollo New Hampshire/Speckled Transylvanian Naked Neck (recipient/donor), respectively. Only animals less than 24. h old from hatching were used as either recipient or donor. Eggs yielding recipients were treated with busulfan to hinder the development of the recipient's own gonads. Gonads were transferred surgically to the new host which was then immunosuppressed for two months. The animals were checked at 8 or 16 weeks for the existence of implanted gonads, and if found, the gonads were removed for histology examination.Tetra SL/Tetra SL pairing resulted in successfully adhered and functioning gonads in most cases. In other combinations, no gonads originating from the donors were found in the recipients. We conclude that not all breeds seem to be suitable recipients; some breeds or individuals may show incompatibility to each other and further examination is needed to find the cause of incompatibility and to establish a suitable breed combination, which can be used for gonad transfer. © 2013 Elsevier B.V.


Varadi E.,Institute for Small Animal Research and Co ordination Center for Gene Conservation | Vegi B.,Institute for Small Animal Research and Co ordination Center for Gene Conservation | Liptoi K.,Institute for Small Animal Research and Co ordination Center for Gene Conservation | Barna J.,Institute for Small Animal Research and Co ordination Center for Gene Conservation
PLoS ONE | Year: 2013

Conservation of indigenous poultry species is an important part of the new Hungarian agricultural strategy. Semen cryopreservation is the most practical method for the long term storage of poultry genetic material. The objective was to compare four protocols for cryopreservation of guinea fowl sperm (slow and fast programmable, freezing in nitrogen vapor, and pellet) and three cryoprotectants (10% ethylene glycol, 6% dimethyl-formamide and 6% dimethyl-acetamide). The efficiency of the methods was examined by in vitro tests (subjective motility scoring, sperm concentration, morphological and live/dead sperm analysis with eosin-aniline staining). Thereafter, the two most promising methods were tested by artificial insemination of frozen-thawed semen (3 times a week for 3 weeks using 300 million spermatozoa/hen), followed by candling of incubated eggs, assessment of fertilization, embryonic death, and hatching rate. The survival rate of live, intact spermatozoa was greatest (p≤0.05) in pellet method and the slow programmable protocol (with 10% ethylene glycol) (28.6 and 23.5%). The two best protocols (based on in vitro assessment of post-thaw semen quality) were subsequently tested in vivo with artificial insemination. The pellet method yielded a 64% fertility rate compared to slow protocol with only 30% fertility. Regardless, both freezing protocols significantly increased embryonic deaths compared to the control group (16,7; 9,1 and 8,3%, respectively). During the 3-week in vivo trial, fertility increased and early embryonic death decreased over time. According to the results the guinea fowl sperm could tolerate the fast freezing in pellet better than the slower freezing rates and resulted acceptable fertility rate. © 2013 Váradi et al.


Eiben C.,Institute for Small Animal Research and Co ordination Center for Gene Conservation | Godor-Surmann K.,Institute for Small Animal Research and Co ordination Center for Gene Conservation | Kustos K.,Laboratory Nyul Ltd.
Livestock Science | Year: 2013

Altered nursing or nutrition before artificial insemination (AI) can be used as a doe biostimulant to improve lactating rabbits reproduction. The timing of a shift from free to a 3-day controlled nursing to AI or the nursing method at fast-refeeding can affect the efficacy of these stimulations. In an earlier study the effects of a 3-day controlled nursing on days 8, 9 and 10 or in controlled nursing rabbits, the impact of a 24-h fast with 48-50. h re-feeding were investigated. This follow-up work tested a 3-day biostimulation with controlled nursing on days 9, 10 and 11. Another aim was to assess the same doe fast-refeeding but now in free-nursing rabbits. Pannon White does (n=480) were artificially inseminated 11 days post-partum. Control (C) does nursed freely. Rabbits simulating local farm practice (F) had controlled nursing until day 14 using a metal-plate as separation and then free nursing to weaning (day 35). In biostimulations with altered nursing, there was a shift from free to a 3-day controlled nursing (days 9-11) with a wire-mesh separation (BW), a metal-plate insertion (BM) or nest-tray removal (BN) and return to free nursing on day 12 until weaning. The C, F, BW, BM and BN does were fed ad libitum. At biostimulation with fast-refeeding (BF), the free-nursing does were subjected to a 24. h water-only fast between days 8 and 9 and a 48-50. h ad libitum re-feeding before AI. Doe reproduction and growth of the current litter were differently affected by the treatments. In the C, F, BW, BM, BN and BF does, sexual receptivity was 83, 90, 68, 80, 74 and 85% (P=0.05), the kindling rate was 79, 76, 74, 89, 68, 70% (P=0.05), the number of kits born alive was 7.9, 8.0, 8.8, 9.1, 7.9, 6.8 (P=0.005), kit weight at weaning 982, 991, 953, 986, 955, 964. g (P=0.012) and at 70 days of age 2383, 2407, 2220, 2350, 2279, 2382. g, respectively (P=0.001). Among biostimulations with altered nursing, the 3-day controlled nursing with a metal-plate separation (BM) can be advised for the practice because only this method was efficient in this (days 9-11) and previous (days 8-10) studies. There appears to be an interaction between doe nursing and feeding, since the same fast led to different production of free-nursing does compared to those in a previous work that nursed controlled. © 2013 Elsevier B.V.


PubMed | Institute for Small Animal Research and Co ordination Center for Gene Conservation
Type: Journal Article | Journal: Animal reproduction science | Year: 2013

The aim of the study was to devise a technique to allow transfer of ovarian and testicular tissues obtained from one day old chicks to recipient animals. The following combinations of chicken breeds were used: Tetra SL/Tetra SL, Tetra SL/Harco, Tetra SL/Black Transylvanian Naked Neck, Godollo New Hampshire/Speckled Transylvanian Naked Neck (recipient/donor), respectively. Only animals less than 24h old from hatching were used as either recipient or donor. Eggs yielding recipients were treated with busulfan to hinder the development of the recipients own gonads. Gonads were transferred surgically to the new host which was then immunosuppressed for two months. The animals were checked at 8 or 16 weeks for the existence of implanted gonads, and if found, the gonads were removed for histology examination. Tetra SL/Tetra SL pairing resulted in successfully adhered and functioning gonads in most cases. In other combinations, no gonads originating from the donors were found in the recipients. We conclude that not all breeds seem to be suitable recipients; some breeds or individuals may show incompatibility to each other and further examination is needed to find the cause of incompatibility and to establish a suitable breed combination, which can be used for gonad transfer.

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