Institute for Problems of Cryobiology and Cryomedicine of the NAS of Ukraine

Kharkiv, Ukraine

Institute for Problems of Cryobiology and Cryomedicine of the NAS of Ukraine

Kharkiv, Ukraine
SEARCH FILTERS
Time filter
Source Type

Rozanova S.L.,Institute for Problems of Cryobiology and Cryomedicine of the NAS of Ukraine | Narozhnyi S.V.,Institute for Problems of Cryobiology and Cryomedicine of the NAS of Ukraine | Nardid O.A.,Institute for Problems of Cryobiology and Cryomedicine of the NAS of Ukraine
Fizika Nizkikh Temperatur | Year: 2017

The purpose of the present work was to investigate influence of different freeze-thawing protocols on structure and antioxidant properties of isolated proteins. In our experiments we have studied human serum albumin, human hemoglobin and cytochrome C derived from equine heart frozen down to 77.15 K with 1-2 deg/min and 300 deg/min rate with following thawing on a water bath at 293.15 K. Native proteins were assumed as a control. Influence of freeze-thawing protocols on protein structure was investigated using spectro-photometric and fluorescent assays. Antioxidant activities of isolated proteins were estimated by their ability to reduce ABTS+ radical. It has been established that unfolding derived from freeze-thawing exposure leads to protein antioxidant activity increasing while decreasing of such an activity may be connected with macromolecule aggregation. Character of freeze-thawing influence on antioxidant activity of proteins depends on molecule structure peculiarities and freezing protocols. © S.L. Rozanova, S.V. Narozhnyi, and O.A. Nardid, 2017.


Goltsev A.N.,Institute for Problems of Cryobiology and Cryomedicine of the NAS of Ukraine | Babenko N.N.,Institute for Problems of Cryobiology and Cryomedicine of the NAS of Ukraine | Gaevskaya Yu.A.,Institute for Problems of Cryobiology and Cryomedicine of the NAS of Ukraine | Chelombytko O.V.,Institute for Problems of Cryobiology and Cryomedicine of the NAS of Ukraine | And 7 more authors.
Genes and Cells | Year: 2015

An actual task of current oncology is the search for the structures, enabling to selectively affect the tumor stem cells, on the expansion rate of those the activity of oncologic process depends. Perspective in this aspect is the use of nanostructures selectively recognizing and inactivating tumor stem cells. In the research there was studied the effect of synthesized hybrid nanocomplexes based on nanoparticles of rare earth orthovanadates GdYVO4:Eu3+, cholesterol and hydrophobic luminescent stain Dil on functional activity of Ehrlich carcinoma cells and expression in them of nanog, oct-4, sox-2 genes. After pre-Treatment of Ehrlich carcinoma cells with nanocomplexes there was shown an inhibition of tumor growth in vivo due to inactivation of the most carcinogenic CD44hi cells, which was accompanied with the reduced expression rate of the studied genes in total pool of cells. The findings contribute to the understanding of the effect mechanisms of orthovanadates and open the prospects to apple new forms of nanocomposites in treatment of oncology diseases.


Sandomirsky B.P.,Institute for Problems of Cryobiology and Cryomedicine of the NAS of Ukraine | Repin N.V.,Institute for Problems of Cryobiology and Cryomedicine of the NAS of Ukraine | Mikhailova I.P.,Institute for Problems of Cryobiology and Cryomedicine of the NAS of Ukraine | Manchenko A.A.,Institute for Problems of Cryobiology and Cryomedicine of the NAS of Ukraine
International Journal of Artificial Organs | Year: 2016

Introduction: The project goal was to study special aspects of biointegration and the functional efficiency of the modified xenopericardium on the experimental model in vivo. Xenopericardium devitalization was performed using low temperatures and ionizing radiation (β-radiation) in an original manner. Methods: In rabbits, a urinary bladder (UB) wall defect was repaired through tissue replacement. Observation period: up to 1 year. After implantation, tissue reaction and biomaterial structure changes were studied using light and electron microscopy. Dynamic ultrasound diagnostics were performed. Results: After surgery all animals displayed normal physiological activity. No cases of material rejection or postoperative period complications were detected. At all stages the xenopericardium preserved structural integrity and served as a skeleton for forming an adequate UB wall. Small peripheral areas were exposed to lysis. After 3 months the collagen structure of the graft was rearranged and mucous membrane epithelization appeared. Folding was already formed and invasion of narrow SMC panniculi was registered within deeper layers. After 6 months the inner surface of the UB wall consisted of normal mucosa, lined by fully formed epithelium. By the 7th month an almost solid muscular sheet was formed in the lower layers. Conclusions: Modified pericardium tissue with the given physical and mechanical properties was sustainable and able to endure the work load in an aggressive environment. It causes minimal inflammatory response, has a potential for cellular repopulation in vivo, stimulates formation of fibrous tissue, and embedded and restored cellular integrity and UB storage function. © 2016 Wichtig Publishing.


PubMed | Institute for Problems of Cryobiology and Cryomedicine of the NAS of Ukraine
Type: Journal Article | Journal: The International journal of artificial organs | Year: 2016

The project goal was to study special aspects of biointegration and the functional efficiency of the modified xenopericardium on the experimental model in vivo. Xenopericardium devitalization was performed using low temperatures and ionizing radiation (--radiation) in an original manner.In rabbits, a urinary bladder (UB) wall defect was repaired through tissue replacement. Observation period: up to 1 year. After implantation, tissue reaction and biomaterial structure changes were studied using light and electron microscopy. Dynamic ultrasound diagnostics were performed.After surgery all animals displayed normal physiological activity. No cases of material rejection or postoperative period complications were detected. At all stages the xenopericardium preserved structural integrity and served as a skeleton for forming an adequate UB wall. Small peripheral areas were exposed to lysis. After 3 months the collagen structure of the graft was rearranged and mucous membrane epithelization appeared. Folding was already formed and invasion of narrow SMC panniculi was registered within deeper layers. After 6 months the inner surface of the UB wall consisted of normal mucosa, lined by fully formed epithelium. By the 7th month an almost solid muscular sheet was formed in the lower layers.Modified pericardium tissue with the given physical and mechanical properties was sustainable and able to endure the work load in an aggressive environment. It causes minimal inflammatory response, has a potential for cellular repopulation in vivo, stimulates formation of fibrous tissue, and embedded and restored cellular integrity and UB storage function.

Loading Institute for Problems of Cryobiology and Cryomedicine of the NAS of Ukraine collaborators
Loading Institute for Problems of Cryobiology and Cryomedicine of the NAS of Ukraine collaborators