Gordiyenko O.I.,Institute for Problems of Cryobiology and Cryomedicine NAS of Ukraine |
Barannyk M.O.,National University of Pharmacy
Journal of Adhesion Science and Technology | Year: 2016
The process of bacterial adhesion is usually discussed in terms of a two-stage sorption model. According to this model, at the first stage bacteria rapidly attach to the surface by weak physical interactions, while at the second stage irreversible molecular and cellular adhesion processes take place. An important factor, influencing the adhesion processes, is physical and chemical characteristics of the medium, in particular, the presence of monovalent and bivalent cations. In this work, we assessed the role of electrostatic component of the intercellular interactions in the media with different electrolyte concentrations (namely, 1:1 and 2:1) at the first reversible stage of adhesion and probability of further specific binding. We compared experimental data of lactobacilli Streptococcus thermophilus adhesion to human erythrocytes with theoretical Debye radius and surface potential of erythrocytes in the experimental solutions of 1:1 electrolyte. Our results showed that with decreasing ionic strength of the solution, the change in the adhesion index in our experiments is fully in line with the theory DLVO (Derjaguin–Landau–Verwey–Overbeek) predictions. The experimental results were obtained and theoretical calculations of the electrostatic interactions parameters in the experimental solutions of 2:1 electrolyte once again confirmed the acceptability of a two-stage model of sorption and DLVO theory to describe a cell–cell adhesion. © 2016 Informa UK Limited, trading as Taylor & Francis Group
Anikieieva M.O.,National University of Pharmacy |
Rozanova S.L.,Institute for Problems of Cryobiology and Cryomedicine NAS of Ukraine |
Kovalenko S.Y.,Institute for Problems of Cryobiology and Cryomedicine NAS of Ukraine |
Kovalenko I.F.,Institute for Problems of Cryobiology and Cryomedicine NAS of Ukraine |
And 2 more authors.
Journal of Adhesion Science and Technology | Year: 2015
We propose a simple and accessible model of lactobacilli adhesion to human erythrocytes. The influence of bivalent cations on the surface charge of erythrocytes and lactobacilli Streptococcus thermophilus and their adhesive interaction has been investigated. We have shown that, despite the seemingly similar unidirectional effect of Ca2+ and Mg2+ cations, the cause of decreased adhesion lactobacilli S. thermophilus to human erythrocytes differ. While Ca2+ ions affect the surface charge of erythrocytes, not changing it in S. thermophilus, Mg2+ ions, conversely, affect the surface charge of lactobacilli and do not change it in erythrocytes. Our results confirm our proposition that in this case, the bivalent cations affect the second irreversible stage of adhesion process, but not the physical interactions during the first reversible stage. © 2015 Taylor & Francis.
Sukach A.N.,Institute for Problems of Cryobiology and Cryomedicine NAS of Ukraine |
Sukach A.N.,National Pedagogical University of Ukraine |
Lebedinsky A.S.,Institute for Problems of Cryobiology and Cryomedicine NAS of Ukraine |
Grishenko V.I.,Institute for Problems of Cryobiology and Cryomedicine NAS of Ukraine |
Lyashenko T.D.,National Pedagogical University of Ukraine
Tsitologiya | Year: 2011
This study shows that toxic effect of non-modified nanoparticles of Fe 3O 4 in vitro depends on metabolic and morphological condition of cells, derived from fetuses and newborn rats. During the cultivation of cells with magnetic nanoparticles, the new-born rats. During the cultivation of cells with magnetic nanoparticles, the latter bind to the cell surface and penetrate into the intracellular space. In this case, sorption of nanoparticles on the cell surface makes it difficult for cells to adhere to substrate, and the absorption by spread cells may prevent their proliferation. Magnetic nanoparticles are well absorbed by the upper layer of cellular aggregates. In this case, the cells of the inner layer remain intact. Consequently, the cell aggregates are able to respond to the constant magnetic field. These aggregates could potentially by used in cell transplantation for directed cell delivery.
Gulevsky A.K.,Institute for Problems of Cryobiology and Cryomedicine NAS of Ukraine |
Trifonova A.V.,Institute for Problems of Cryobiology and Cryomedicine NAS of Ukraine |
Lavrik A.A.,State Research Control Institute of Biotechnology and Microorganisms Strains
Tsitologiya | Year: 2011
The influence of the cattle cord blood fraction with molecular weight of components below 5 kDa on adhesive and proliferative properties of continuous cell lines BHK-21 clone q3/04 and PK-15-IECVM was investigated in comparison with the preparation «Actovegin». It was shown that adding this fraction as well as «Actovegin » to growth media did not affect the efficiency of culture attachment, promoted culture spreading, improved cell morphology and stimulated mitotic activity of the cultures. The efficiency of the cord blood fraction below 5 kDa estimated by the induces studied was found to be higher than the «Actovegin» efficiency when cells being cultivated in the media different contents of blood serum.
Anikieieva M.,University of Kharkiv |
Gordiyenko O.,Institute for Problems of Cryobiology and Cryomedicine NAS of Ukraine
Periodicum Biologorum | Year: 2014
Background and Purpose: Diseases caused by bacterial pathogens are the result of the host-pathogen interaction, therefore the contact of bacteria with erythrocytes is highly possible. The aim of this work was to study Streptococcus thermophilus adhesion on human erythrocytes depending on the various pH and ionic strength of the medium. Materials and Methods: Streptococcus thermophilus bacterial cells and human red blood cells have been selected. Erythrocytes were washed with buffer saline and precipitated by centrifugation. Dried bacterial cells were suspended, incubated, washed in buffer saline and precipitated. The precipitate of both types of cells was resuspended in 1:2 ratio in a buffer solution with different physicochemical characteristics. The number of bacteria adhered to each erythrocyte has been counted under light microscope in each field of view and adhesion coefficient has been calculated. Results: Adhesion coefficient is strongly dependent on pH of the medium and reaches maximum at physiological pH for red blood cells. Data for the other examined pH values probabilistically differ at the same confidence level. Adhesion index was the highest in physiological saline (2.21 ± 0.87). Both increase or decrease in the ionic strength (0.2 M salt) resulted in the drop of adhesion rate, it becomes minimal (0.95 ± 0.63) in the medium with the lowest investigated ionic strength (0.25 M sucrose, 0.025 M salt). Conclusions: The results of our experiments indicate that adhesion proceeds through initial nonspecific stage which plays an important role in the adhesion process and affects the possibility of the second irreversible stage of adhesion.