Time filter

Source Type

Kucherenko Y.V.,Institute for Problems of Cryobiology and Cryomedicine | Lang F.,University of Tübingen
Journal of Membrane Biology | Year: 2014

Non-steroidal anti-inflammatory drugs (NSAIDs) are known to induce apoptosis in a variety of cancer cells. However, the precise mechanisms by which NSAIDs facilitate apoptosis in tumor cells are not clear. In the present study, we show that niflumic acid (NA), a member of the fenamates group of NSAIDs and Cl- and Ca2+-activated Cl- (CAC) channels blocker, induced apoptosis (by ∼8 %, 24 h treatment) and potentiated (by 8-10 %) apoptotic effect of endoplasmic reticulum Ca2+ mobilizer thapsigargin (Tg) in human erythroleukemic K562 cell line. The whole-cell patch clamp and Fluo-3 flow cytometric experiments confirmed an inhibitory effect of NA (100 and 300 μM) on store-operated (SOC) channels. We also found that NA-blocked CAC channels were activated by acute application of Tg (2 μM) in K562 cells. NA blockage of CAC channels was accompanied by activation of Ca 2+-activated K+ (SK4) channels. The observed effects of NA were not connected with COX-2 inhibition since 100-nM NA (IC50 for COX-2 inhibition) did not induce either apoptosis or affect the channels activity. We conclude that inhibition of SOC channels plays a major role in NA-induced apoptosis. Increased apoptotic levels in Tg-treated K562 cells in the presence of NA may be due to the blockage of CAC and stimulation of SK4 channels in addition to SOC channels inhibition. © 2014 Springer Science+Business Media.


Petrenko A.Y.,Institute for Problems of Cryobiology and Cryomedicine | Cherkashina D.V.,Institute for Problems of Cryobiology and Cryomedicine | Somov A.Y.,Institute for Problems of Cryobiology and Cryomedicine | Somov A.Y.,University College London | And 4 more authors.
Cryobiology | Year: 2010

Reversible uncoupling of the mitochondrial electron-transport chain may be one strategy to prevent intracellular oxidative stress during liver cold preservation/warm reperfusion (CP/WR) injury. 2,4-Dinitrophenol (DNP) is a potent water-soluble uncoupling agent for supplementation of the hepatic CP solution. The aim of this work was to investigate the possible influence of DNP in the CP solution on the isolated rat liver state during CP/WR. Livers were subjected to CP at 4°C in sucrose-phosphate based solution (SPS) for 18h, followed by WR for 60min in vitro. The final concentration of DNP was 100μM. DNP presence during the CP stage led to partial ATP level decrease accompanied by a significant diminution in liver TBARS and a prevention of antioxidant enzyme activity decrease (catalase, GSH-PO, GSH-Red) when compared with livers stored without DNP. After DNP wash-out during WR, an improvement in the mitochondrial functional state (higher respiratory control indices and ATP levels, and a decrease in V4 respiration rates) were observed. This was concurrent with lower TBARS levels, higher antioxidant enzyme activities and significant increase of bile production. The results suggest that reversible uncoupling may be one way to influence oxidative stress associated with hepatic cold preservation. © 2010 Elsevier Inc.


Goltsev A.N.,Institute for Problems of Cryobiology and Cryomedicine | Rassokha I.V.,Institute for Problems of Cryobiology and Cryomedicine | Dubrava T.G.,Institute for Problems of Cryobiology and Cryomedicine | Ostankova L.V.,Institute for Problems of Cryobiology and Cryomedicine | And 4 more authors.
Cellular Transplantation and Tissue Engineering | Year: 2013

The results of experimental research for the effect character of nanocomposite coatings with different physicochemical parameters on structural and functional properties (adhesive potential, phenotype, expression of ido gene) of multipotent mesenchymal stromal cells (MMSCs) are presented in the work. In the whole spectrum of tested nanocoatings (Al2O3, ZrO2, Ta2O5) we have established the ability of oxide coating Al2O3 to monolayerly enrich the cultured in vitro bone marrow (BM) with the MMSCs and increase the degree of ido expression. This can expand the spectrum and give them new shapes of therapeutic potential in clinical practice. © Human stem cells institute, 2013.


Iukhta M.S.,Institute for Problems of Cryobiology and Cryomedicine | Volkova N.A.,Institute for Problems of Cryobiology and Cryomedicine | Zhulikova E.P.,Institute for Problems of Cryobiology and Cryomedicine | Goncharuk E.I.,Institute for Problems of Cryobiology and Cryomedicine
Cellular Transplantation and Tissue Engineering | Year: 2013

The choice object for cell therapy of degenerative changes of the intervertebral discs is a cryopreserved multipotent mesenchymal stromal cells (MMSC). The research aim was to evaluate a therapeutic potential of cryopreserved allogenic marrow-derived MMSC in this pathology. Rats with modeled compressive degenerative damage of the intervertebral disc CcVI-VII were introduced by 0.5×106 native or cryopreserved as well as PKH-26-labeled cells on a collagen sponge in the defect area. Animals with spontaneous recovery and administration of saline were served as a control. Histomorphometric study and fluorescent microscopy of intervertebral disc sections was performed on the 30th day after treatment. There was extremely low regenerative potential in the control groups of animals. Histologically after cell therapy, there was tendency to repair of cracks, fissures, collagen fiber fragmentations of the fibrous ring, which was more pronounced in the case of the native MMSC culture application. The cryopreserved MMSC administration was accompanied by increasing in the fibrochondrocyte number of the dorsal annulus per unit area of the intervertebral disc CcVI-VII slice at 1.25 times relative to the model values. At the same time the fibrous ring height increased by 12.5±3.3%, and densitometric index of cartilaginous tissue - by 64±5.7% relative to the model values. The luminescence in the red range of the spectrum in the form of drops and conglomerates, which was localized in outer parts of fibrous ring, was detected by fluorescent microscopy of intervertebral disc cryostat sections on the 30 day after introduction of labeled native or cryopreserved MMSC, that indicated the safety and partial migration of transplanted and/ or their daughter cells in the intervertebral disc CcVI-VII. Our data showed a stimulating effect of native and cryopreserved marrow-derived MMSC suspension in degenerative intervertebral disc damages.


Grzhibovskis R.,Saarland University | Kramer E.,Saarland University | Bernhardt I.,Saarland University | Kemper B.,University of Munster | And 4 more authors.
European Biophysics Journal | Year: 2016

The phenomenon of physical contact between red blood cells and artificial surfaces is considered. A fully three-dimensional mathematical model of a bilayer membrane in contact with an artificial surface is presented. Numerical results for the different geometries and adhesion intensities are found to be in agreement with experimentally observed geometries obtained by means of digital holographic microscopy. © 2016 European Biophysical Societies' Association


PubMed | Institute for Problems of Cryobiology and Cryomedicine, Kharkiv Polytechnic Institute, Saarland University, University of Munster and Chalmers University of Technology
Type: | Journal: European biophysics journal : EBJ | Year: 2016

The phenomenon of physical contact between red blood cells and artificial surfaces is considered. A fully three-dimensional mathematical model of a bilayer membrane in contact with an artificial surface is presented. Numerical results for the different geometries and adhesion intensities are found to be in agreement with experimentally observed geometries obtained by means of digital holographic microscopy.


PubMed | Ukrainian Academy of Sciences and Institute for Problems of Cryobiology and Cryomedicine
Type: Journal Article | Journal: Cryo letters | Year: 2017

Cryopreservation of mesenchymal stromal cells (MSCs) and MSCs-based tissue engineered constructs (TECs) is a promising strategy for regenerative medicine.To examine vitrification system consisting of multicomponent vitreous solution, closed type container, human adult MSCs and two-step exposure procedure as a platform for cryopreservation of MSCs-based TECs.Vitrification properties of solutions were studied by visual analysis and calorimetry. Viability (trypan blue, MTT-test), metabolic activity (Alamar Blue assay) and adhesion of cells were assessed both after exposure with vitreous solutions and following rapid cooling-thawing in standard cryovials.The feasibility of the vitrification system was tested on MSCs suspensions (S-MSCs) and alginate encapsulated MSCs (AE-MSCs). The minimal concentrations of cryoprotectants, which allowed avoiding ice formation during rapid cooling and rewarming comprised 10 % for dimethylsulfoxide, 20 % for ethylene glycol, 20 % for 1.2-propanediol and 0.5 M sucrose. To achieve viability and metabolic activity rates of AE-MSCs comparable to S-MSCs after vitrification the extension of the exposure time within the same vitreous solution was sufficient. After vitrification both S-MSCs and AE-MSCs retained the capacity to osteogenic and adipogenic differentiation.Data demonstrate that this vitrification system can be used as a platform for development of effective protocols for cryopreservation of MSCs-based TECs.


PubMed | Institute for Problems of Cryobiology and Cryomedicine
Type: Journal Article | Journal: Cryobiology | Year: 2010

Reversible uncoupling of the mitochondrial electron-transport chain may be one strategy to prevent intracellular oxidative stress during liver cold preservation/warm reperfusion (CP/WR) injury. 2,4-Dinitrophenol (DNP) is a potent water-soluble uncoupling agent for supplementation of the hepatic CP solution. The aim of this work was to investigate the possible influence of DNP in the CP solution on the isolated rat liver state during CP/WR. Livers were subjected to CP at 4 degrees C in sucrose-phosphate based solution (SPS) for 18 h, followed by WR for 60 min in vitro. The final concentration of DNP was 100 microM. DNP presence during the CP stage led to partial ATP level decrease accompanied by a significant diminution in liver TBARS and a prevention of antioxidant enzyme activity decrease (catalase, GSH-PO, GSH-Red) when compared with livers stored without DNP. After DNP wash-out during WR, an improvement in the mitochondrial functional state (higher respiratory control indices and ATP levels, and a decrease in V(4) respiration rates) were observed. This was concurrent with lower TBARS levels, higher antioxidant enzyme activities and significant increase of bile production. The results suggest that reversible uncoupling may be one way to influence oxidative stress associated with hepatic cold preservation.

Loading Institute for Problems of Cryobiology and Cryomedicine collaborators
Loading Institute for Problems of Cryobiology and Cryomedicine collaborators