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Kucherenko Y.V.,Institute for Problems of Cryobiology and Cryomedicine | Lang F.,University of Tubingen
Journal of Membrane Biology | Year: 2014

Non-steroidal anti-inflammatory drugs (NSAIDs) are known to induce apoptosis in a variety of cancer cells. However, the precise mechanisms by which NSAIDs facilitate apoptosis in tumor cells are not clear. In the present study, we show that niflumic acid (NA), a member of the fenamates group of NSAIDs and Cl- and Ca2+-activated Cl- (CAC) channels blocker, induced apoptosis (by ∼8 %, 24 h treatment) and potentiated (by 8-10 %) apoptotic effect of endoplasmic reticulum Ca2+ mobilizer thapsigargin (Tg) in human erythroleukemic K562 cell line. The whole-cell patch clamp and Fluo-3 flow cytometric experiments confirmed an inhibitory effect of NA (100 and 300 μM) on store-operated (SOC) channels. We also found that NA-blocked CAC channels were activated by acute application of Tg (2 μM) in K562 cells. NA blockage of CAC channels was accompanied by activation of Ca 2+-activated K+ (SK4) channels. The observed effects of NA were not connected with COX-2 inhibition since 100-nM NA (IC50 for COX-2 inhibition) did not induce either apoptosis or affect the channels activity. We conclude that inhibition of SOC channels plays a major role in NA-induced apoptosis. Increased apoptotic levels in Tg-treated K562 cells in the presence of NA may be due to the blockage of CAC and stimulation of SK4 channels in addition to SOC channels inhibition. © 2014 Springer Science+Business Media. Source

Grzhibovskis R.,Saarland University | Kramer E.,Saarland University | Bernhardt I.,Saarland University | Kemper B.,University of Munster | And 4 more authors.
European Biophysics Journal | Year: 2016

The phenomenon of physical contact between red blood cells and artificial surfaces is considered. A fully three-dimensional mathematical model of a bilayer membrane in contact with an artificial surface is presented. Numerical results for the different geometries and adhesion intensities are found to be in agreement with experimentally observed geometries obtained by means of digital holographic microscopy. © 2016 European Biophysical Societies' Association Source

Goltsev A.N.,Institute for Problems of Cryobiology and Cryomedicine | Rassokha I.V.,Institute for Problems of Cryobiology and Cryomedicine | Dubrava T.G.,Institute for Problems of Cryobiology and Cryomedicine | Ostankova L.V.,Institute for Problems of Cryobiology and Cryomedicine | And 4 more authors.
Cellular Transplantation and Tissue Engineering | Year: 2013

The results of experimental research for the effect character of nanocomposite coatings with different physicochemical parameters on structural and functional properties (adhesive potential, phenotype, expression of ido gene) of multipotent mesenchymal stromal cells (MMSCs) are presented in the work. In the whole spectrum of tested nanocoatings (Al2O3, ZrO2, Ta2O5) we have established the ability of oxide coating Al2O3 to monolayerly enrich the cultured in vitro bone marrow (BM) with the MMSCs and increase the degree of ido expression. This can expand the spectrum and give them new shapes of therapeutic potential in clinical practice. © Human stem cells institute, 2013. Source

Iukhta M.S.,Institute for Problems of Cryobiology and Cryomedicine | Volkova N.A.,Institute for Problems of Cryobiology and Cryomedicine | Zhulikova E.P.,Institute for Problems of Cryobiology and Cryomedicine | Goncharuk E.I.,Institute for Problems of Cryobiology and Cryomedicine
Cellular Transplantation and Tissue Engineering | Year: 2013

The choice object for cell therapy of degenerative changes of the intervertebral discs is a cryopreserved multipotent mesenchymal stromal cells (MMSC). The research aim was to evaluate a therapeutic potential of cryopreserved allogenic marrow-derived MMSC in this pathology. Rats with modeled compressive degenerative damage of the intervertebral disc CcVI-VII were introduced by 0.5×106 native or cryopreserved as well as PKH-26-labeled cells on a collagen sponge in the defect area. Animals with spontaneous recovery and administration of saline were served as a control. Histomorphometric study and fluorescent microscopy of intervertebral disc sections was performed on the 30th day after treatment. There was extremely low regenerative potential in the control groups of animals. Histologically after cell therapy, there was tendency to repair of cracks, fissures, collagen fiber fragmentations of the fibrous ring, which was more pronounced in the case of the native MMSC culture application. The cryopreserved MMSC administration was accompanied by increasing in the fibrochondrocyte number of the dorsal annulus per unit area of the intervertebral disc CcVI-VII slice at 1.25 times relative to the model values. At the same time the fibrous ring height increased by 12.5±3.3%, and densitometric index of cartilaginous tissue - by 64±5.7% relative to the model values. The luminescence in the red range of the spectrum in the form of drops and conglomerates, which was localized in outer parts of fibrous ring, was detected by fluorescent microscopy of intervertebral disc cryostat sections on the 30 day after introduction of labeled native or cryopreserved MMSC, that indicated the safety and partial migration of transplanted and/ or their daughter cells in the intervertebral disc CcVI-VII. Our data showed a stimulating effect of native and cryopreserved marrow-derived MMSC suspension in degenerative intervertebral disc damages. Source

Petrenko A.Y.,Institute for Problems of Cryobiology and Cryomedicine | Cherkashina D.V.,Institute for Problems of Cryobiology and Cryomedicine | Somov A.Y.,Institute for Problems of Cryobiology and Cryomedicine | Somov A.Y.,University College London | And 4 more authors.
Cryobiology | Year: 2010

Reversible uncoupling of the mitochondrial electron-transport chain may be one strategy to prevent intracellular oxidative stress during liver cold preservation/warm reperfusion (CP/WR) injury. 2,4-Dinitrophenol (DNP) is a potent water-soluble uncoupling agent for supplementation of the hepatic CP solution. The aim of this work was to investigate the possible influence of DNP in the CP solution on the isolated rat liver state during CP/WR. Livers were subjected to CP at 4°C in sucrose-phosphate based solution (SPS) for 18h, followed by WR for 60min in vitro. The final concentration of DNP was 100μM. DNP presence during the CP stage led to partial ATP level decrease accompanied by a significant diminution in liver TBARS and a prevention of antioxidant enzyme activity decrease (catalase, GSH-PO, GSH-Red) when compared with livers stored without DNP. After DNP wash-out during WR, an improvement in the mitochondrial functional state (higher respiratory control indices and ATP levels, and a decrease in V4 respiration rates) were observed. This was concurrent with lower TBARS levels, higher antioxidant enzyme activities and significant increase of bile production. The results suggest that reversible uncoupling may be one way to influence oxidative stress associated with hepatic cold preservation. © 2010 Elsevier Inc. Source

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