Institute for Physical Chemical Medicine of Ministry of Public Health

Moscow, Russia

Institute for Physical Chemical Medicine of Ministry of Public Health

Moscow, Russia
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Varizhuk A.M.,RAS Engelhardt Institute of Molecular Biology | Kaluzhny D.N.,RAS Engelhardt Institute of Molecular Biology | Novikov R.A.,RAS Engelhardt Institute of Molecular Biology | Chizhov A.O.,RAS N. D. Zelinsky Institute of Organic Chemistry | And 6 more authors.
Journal of Organic Chemistry | Year: 2013

New oligonucleotide analogues with triazole internucleotide linkages were synthesized, and their hybridization properties were studied. The analogues demonstrated DNA binding affinities similar to those of unmodified oligonucleotides. The modification was shown to protect the oligonucleotides from nuclease hydrolysis. The modified oligonucleotides were tested as PCR primers. Modifications remote from the 3′-terminus were tolerated by polymerases. Our results suggest that these new oligonucleotide analogues are among the most promising triazole DNA mimics characterized to date. © 2013 American Chemical Society.


PubMed | Institute for Physical Chemical Medicine of Ministry of Public Health
Type: Journal Article | Journal: The Journal of organic chemistry | Year: 2013

New oligonucleotide analogues with triazole internucleotide linkages were synthesized, and their hybridization properties were studied. The analogues demonstrated DNA binding affinities similar to those of unmodified oligonucleotides. The modification was shown to protect the oligonucleotides from nuclease hydrolysis. The modified oligonucleotides were tested as PCR primers. Modifications remote from the 3-terminus were tolerated by polymerases. Our results suggest that these new oligonucleotide analogues are among the most promising triazole DNA mimics characterized to date.

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