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Apeldoorn, Netherlands

Chokkalingam V.,Institute for Molecules and Materials | Ma Y.,Institute for Molecules and Materials | Thiele J.,Institute for Molecules and Materials | Schalk W.,Institute for Molecules and Materials | And 2 more authors.
Lab on a Chip - Miniaturisation for Chemistry and Biology | Year: 2014

Droplet-based microfluidics is increasingly used for biological applications, where the recovery of cells or particles after an experiment or assay is desirable. Here, we present an electro-demulsification chip which circumvents the use of harsh chemicals and multiple washing/centrifugation steps and offers a mild way for extracting cells and polymer particles into an aqueous phase from microfluidic water-in-oil emulsions. This journal is © the Partner Organisations 2014.

Jin Y.,Institute for Molecules and Materials | Cristescu S.M.,Institute for Molecules and Materials | Harren F.J.M.,Institute for Molecules and Materials | Mandon J.,Institute for Molecules and Materials
CLEO: Applications and Technology, CLEO-AT 2015 | Year: 2015

We present the observation of several nonlinear effects in a two-crystal femtosecond optical parametric oscillator. Unusual parametric processes are observed when the total cavity length is detuned up to 7.3 mm within this synchronously pumped optical parametric oscillator. © 2015 OSA.

Thiele J.,Institute for Molecules and Materials | Ma Y.,Institute for Molecules and Materials | Bruekers S.M.C.,Institute for Molecules and Materials | Ma S.,University of Cambridge | Huck W.T.S.,Institute for Molecules and Materials
Advanced Materials | Year: 2014

Cell culturing, whether for tissue engineering or cell biology studies, always involves placing cells in a non-natural environment and no material currently exist that can mimic the entire complexity of natural tissues and variety of cell-matrix interactions that is found in vivo. Here, we review the vast range of hydrogels, composed of natural or synthetic polymers that provide a route to tailored microenvironments. This review surveys the current developments for hydrogels that are fabricated from natural and synthetic polymers for use as tailored cell culture materials. By focusing on chemical functionalization, physical manipulation, and material properties, this review serves as a guide to select hydrogel materials to answer specific questions in cell biology. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Leunissen E.H.P.,Institute for Molecules and Materials | Leunissen E.H.P.,Radboud University Nijmegen | Meuleners M.H.L.,Institute for Molecules and Materials | Meuleners M.H.L.,Radboud University Nijmegen | And 4 more authors.
ChemBioChem | Year: 2014

The ability of cells to incorporate azidosugars metabolically is a useful tool for extracellular glycan labelling. The exposed azide moiety can covalently react with alkynes, such as bicyclo[6.1.0]nonyne (BCN), by strain-promoted alkyne-azide cycloaddition (SPAAC). However, the use of SPAAC can be hampered by low specificity of the cycloalkyne. In this article we describe the synthesis of more polar BCN derivatives and their properties for selective cellular glycan labelling. The new polar derivatives [amino-BCN, glutarylamino-BCN and bis(hydroxymethyl)-BCN] display reaction rates similar to those of BCN and are less cell-permeable. The labelling specificity in HEK293 cells is greater than that of BCN, as determined by confocal microscopy and flow cytometry. Interestingly, amino-BCN appears to be highly specific for the Golgi apparatus. In addition, the polar BCN derivatives label the N-glycan of the membrane calcium channel TRPV5 in HEK293 cells with significantly enhanced signal-to-noise ratios. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Ma Y.,Institute for Molecules and Materials | Thiele J.,Institute for Molecules and Materials | Abdelmohsen L.,Institute for Molecules and Materials | Xu J.,Institute for Molecules and Materials | Huck W.T.S.,Institute for Molecules and Materials
Chemical Communications | Year: 2013

A series of water-soluble macro-initiators is synthesized to avoid radical loss in microfluidic on-chip photo cross-linking of hyaluronic acid methacrylate-containing water-in-oil emulsions. Their superior performance over known photo-initiators through the generation of water-soluble radicals and excellent biocompatibility are demonstrated. © 2014 The Royal Society of Chemistry.

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