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Kardum N.,Institute for Medical Research | Konic-Ristic A.,Institute for Medical Research | Savikin K.,Institute for Medicinal Plants Research | Spasic S.,University of Belgrade | And 3 more authors.
Journal of Medicinal Food | Year: 2014

Berry fruits are a rich source of polyphenols, especially anthocyanins: well-known potent anti-oxidant phytochemicals. The purpose of this study was to evaluate beneficial effects of long-term consumption of polyphenol-rich organic chokeberry juice on different markers of antioxidant/pro-oxidant status in healthy female volunteers. Twenty-nine women, aged 25-49, were included in the study. Serological markers of oxidative stress and antioxidant defence, blood pressure, routine biochemical, and anthropometric parameters were analyzed at baseline and after twelve weeks of regular chokeberry juice consumption. Significant decrease in thiobarbituric acid-reactive substances level (TBARS; P<.001) and pro-oxidant-antioxidant balance (PAB; P<.05), as well as increase in paroxonase-1 activity toward diazoxon (P<.01) were found. Total oxidative status and sulphydryl groups levels were not significantly influenced by the intervention. Anthropometric, biochemical parameters, and blood pressure values were within the referent values for all subjects and were not influenced by the chokeberry juice consumption. However, we found positive correlation between age, body mass index, waist circumference, body fat percent, blood pressure, and analyzed marker of lipid peroxidation, which was influenced by the consumption. In conclusion, the fine modulation of several antioxidant/pro- oxidant status biomarkers observed in healthy subjects indicates putative prophylactic effects of polyphenol-rich chokeberry juice and supports its importance as part of an optimal diet. © 2014, Mary Ann Liebert, Inc. and Korean Society of Food Science and Nutrition. Source


Konic-Ristic A.,Institute for Medical Research | Savikin K.,Institute for Medicinal Plants Research | Zdunic G.,Institute for Medicinal Plants Research | Jankovic T.,Institute for Medicinal Plants Research | And 3 more authors.
Food Chemistry | Year: 2011

Total phenolics, total anthocyanins, mineral content, radical scavenging activity and antiproliferative activity against human cancer cell lines were evaluated in fresh pressed juices of five different berries. Total phenolic content ranged from 133.0 to 260.3mg of gallic acid equivalents/100g of fresh weight, for red currant and black currant, respectively. Bilberry juice contained the highest amount of total anthocyanins (0.18%). Significant correlation between total phenolics content and radical scavenging activity was observed (r=-0.980; p<0.01). All examined juices showed antiproliferative activity in dose-dependent manner with IC50 ranging from 10.2 to 70.5μl/ml. Black currant juice was the most effective inhibitor of proliferation in all cell lines tested (HeLa, Fem X, LS 174, MCF-7 and PC-3). Significant correlations of acidity and total anthocyanin content with antiproliferative activity of berry juices on HeLa cells, Fem X cells and MCF-7 cells were noticed. Berry juices are good sources of some minerals and contribute significantly to daily intake of these micronutrients. © 2010 Elsevier Ltd. Source


Savikin K.P.,Institute for Medicinal Plants Research | Menkovic N.R.,Institute for Medicinal Plants Research | Zdunic G.M.,Institute for Medicinal Plants Research | Tasic S.R.,Institute for Medicinal Plants Research | And 3 more authors.
Journal of Essential Oil Research | Year: 2010

Light yellowish, pleasant-smelling essential oils were obtained in 0.3%, 1.3%, and 0.8% yields from the aerial parts of S. cuneifolia Ten., M. thymifolia (Scop.) Fritsch., and M. dalmatica Benth, respectively. Thirty-four compounds were identified in the oil of M. dalmatica representing 98.7% of total oil composition, while in the oil of M. thymifolia, 21 were identified representing 78.2% of total oil composition. The major compounds in the oil of M. thymifolia included pulegone (50.4%), piperitenone (10.3%) and piperitenone oxide (4.3%), while the oil of M. dalmatica was rich in pulegone (26.7%), piperitenone (21.8%) and piperitenone oxide (25.4%). Fifty-five compounds were characterized in the oil of S. cuneifolia, representing 92.1% of total oil composition. Linalool (19.9%) and α-pinene (12.3%) were found to be the dominant compounds. Antimicrobial activity was noticed in all examined oils with MIC values ranging from 5.6-54 μg/mL. The most active was the oil of S. cuneifolia, which completely inhibited the growth of mostly all tested microorganisms at the lowest supplied concentration. Glandular trichomes (both peltate and capitate) and nonglandular, uniseriate, simple hairs were distributed on abaxial and adaxial surface of the leaves, calyces and stem in all examined species. © 2010 Allured Business Media. Source


Stevic T.,Institute for Medicinal Plants Research | Savikin K.,Institute for Medicinal Plants Research | Ristic M.,Institute for Medicinal Plants Research | Zdunic G.,Institute for Medicinal Plants Research | And 3 more authors.
Journal of the Serbian Chemical Society | Year: 2010

The essential oil from the leaves of the Serbian black currant cultivar Čačanska crna, obtained by hydrodistillation, was analyzed by gas chromatography-flame ionization detection and GC-mass spectrometry. The most abundant volatile compounds were Δ3-carene (18.7 %), β-caryophyllene (17.7 %), sabinene (11.6 %), cis-β-ocimene (10.6 %) and α-terpinolene (10.6 %). The antimicrobial activity of the oil was evaluated against 14 micro-organisms, including two clinical isolated strains, using the broth microdilution method. The most susceptible micro-organisms were Escherichia coli, Streptococcus faecalis, Staphylococcus aureus, Candida albicans and Trichophyton mentagrophytes isolates. Furthermore, the flavonol aglycones in the leaves after acid hydrolysis were qualitatively and quantitatively analysed by HPLC, and quercetin was found to be the dominant compound (84 mg/g dw). 2009 Copyright (CC) SCS. Source


Kardum N.,University of Belgrade | Milovanovic B.,University of Belgrade | Savikin K.,Institute for Medicinal Plants Research | Zdunic G.,Institute for Medicinal Plants Research | And 3 more authors.
Journal of Medicinal Food | Year: 2015

Epidemiological studies have shown a positive association between intake of foods rich in antioxidants and lower incidence of cardiovascular disease development. Polyphenols are considered the most abundant and important dietary antioxidants. The aim of this study was to evaluate effects of polyphenol-rich chokeberry juice consumption on 24-h ambulatory monitored blood pressure (BP) level in subjects with no pharmacologically treated high normal BP or grade I hypertension. Twenty-three subjects (12 men and 11 women) aged 33-67 were enrolled and instructed to consume 200mL of juice daily for 4 weeks. Participants were divided in two groups, based on prevalence of sympathetic or parasympathetic activity. Measurements of biochemical parameters and heart rate variability analysis were also applied. At the end of the intervention period, average 24-h and awake systolic and diastolic BP were significantly decreased (P<.05). This was more pronounced in the group with prevalence of sympathetic activity. Significant reduction in triglyceride level (P<.05) and a reducing effect on total and low-density lipoprotein cholesterol were also found. Obtained results indicate a positive impact of regular chokeberry juice consumption on BP and lipid status in pharmacologically untreated hypertensive subjects. © Copyright 2015, Mary Ann Liebert, Inc. and Korean Society of Food Science and Nutrition 2015. Source

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