Institute for Medicinal Plants Research

Belgrade, Serbia

Institute for Medicinal Plants Research

Belgrade, Serbia

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Kardum N.,Institute for Medical Research | Konic-Ristic A.,Institute for Medical Research | Savikin K.,Institute for Medicinal Plants Research | Spasic S.,University of Belgrade | And 3 more authors.
Journal of Medicinal Food | Year: 2014

Berry fruits are a rich source of polyphenols, especially anthocyanins: well-known potent anti-oxidant phytochemicals. The purpose of this study was to evaluate beneficial effects of long-term consumption of polyphenol-rich organic chokeberry juice on different markers of antioxidant/pro-oxidant status in healthy female volunteers. Twenty-nine women, aged 25-49, were included in the study. Serological markers of oxidative stress and antioxidant defence, blood pressure, routine biochemical, and anthropometric parameters were analyzed at baseline and after twelve weeks of regular chokeberry juice consumption. Significant decrease in thiobarbituric acid-reactive substances level (TBARS; P<.001) and pro-oxidant-antioxidant balance (PAB; P<.05), as well as increase in paroxonase-1 activity toward diazoxon (P<.01) were found. Total oxidative status and sulphydryl groups levels were not significantly influenced by the intervention. Anthropometric, biochemical parameters, and blood pressure values were within the referent values for all subjects and were not influenced by the chokeberry juice consumption. However, we found positive correlation between age, body mass index, waist circumference, body fat percent, blood pressure, and analyzed marker of lipid peroxidation, which was influenced by the consumption. In conclusion, the fine modulation of several antioxidant/pro- oxidant status biomarkers observed in healthy subjects indicates putative prophylactic effects of polyphenol-rich chokeberry juice and supports its importance as part of an optimal diet. © 2014, Mary Ann Liebert, Inc. and Korean Society of Food Science and Nutrition.


Kardum N.,University of Belgrade | Milovanovic B.,University of Belgrade | Savikin K.,Institute for Medicinal Plants Research | Zdunic G.,Institute for Medicinal Plants Research | And 3 more authors.
Journal of Medicinal Food | Year: 2015

Epidemiological studies have shown a positive association between intake of foods rich in antioxidants and lower incidence of cardiovascular disease development. Polyphenols are considered the most abundant and important dietary antioxidants. The aim of this study was to evaluate effects of polyphenol-rich chokeberry juice consumption on 24-h ambulatory monitored blood pressure (BP) level in subjects with no pharmacologically treated high normal BP or grade I hypertension. Twenty-three subjects (12 men and 11 women) aged 33-67 were enrolled and instructed to consume 200mL of juice daily for 4 weeks. Participants were divided in two groups, based on prevalence of sympathetic or parasympathetic activity. Measurements of biochemical parameters and heart rate variability analysis were also applied. At the end of the intervention period, average 24-h and awake systolic and diastolic BP were significantly decreased (P<.05). This was more pronounced in the group with prevalence of sympathetic activity. Significant reduction in triglyceride level (P<.05) and a reducing effect on total and low-density lipoprotein cholesterol were also found. Obtained results indicate a positive impact of regular chokeberry juice consumption on BP and lipid status in pharmacologically untreated hypertensive subjects. © Copyright 2015, Mary Ann Liebert, Inc. and Korean Society of Food Science and Nutrition 2015.


Konic-Ristic A.,Institute for Medical Research | Savikin K.,Institute for Medicinal Plants Research | Zdunic G.,Institute for Medicinal Plants Research | Jankovic T.,Institute for Medicinal Plants Research | And 3 more authors.
Food Chemistry | Year: 2011

Total phenolics, total anthocyanins, mineral content, radical scavenging activity and antiproliferative activity against human cancer cell lines were evaluated in fresh pressed juices of five different berries. Total phenolic content ranged from 133.0 to 260.3mg of gallic acid equivalents/100g of fresh weight, for red currant and black currant, respectively. Bilberry juice contained the highest amount of total anthocyanins (0.18%). Significant correlation between total phenolics content and radical scavenging activity was observed (r=-0.980; p<0.01). All examined juices showed antiproliferative activity in dose-dependent manner with IC50 ranging from 10.2 to 70.5μl/ml. Black currant juice was the most effective inhibitor of proliferation in all cell lines tested (HeLa, Fem X, LS 174, MCF-7 and PC-3). Significant correlations of acidity and total anthocyanin content with antiproliferative activity of berry juices on HeLa cells, Fem X cells and MCF-7 cells were noticed. Berry juices are good sources of some minerals and contribute significantly to daily intake of these micronutrients. © 2010 Elsevier Ltd.


Stevic T.,Institute for Medicinal Plants Research | Savikin K.,Institute for Medicinal Plants Research | Ristic M.,Institute for Medicinal Plants Research | Zdunic G.,Institute for Medicinal Plants Research | And 3 more authors.
Journal of the Serbian Chemical Society | Year: 2010

The essential oil from the leaves of the Serbian black currant cultivar Čačanska crna, obtained by hydrodistillation, was analyzed by gas chromatography-flame ionization detection and GC-mass spectrometry. The most abundant volatile compounds were Δ3-carene (18.7 %), β-caryophyllene (17.7 %), sabinene (11.6 %), cis-β-ocimene (10.6 %) and α-terpinolene (10.6 %). The antimicrobial activity of the oil was evaluated against 14 micro-organisms, including two clinical isolated strains, using the broth microdilution method. The most susceptible micro-organisms were Escherichia coli, Streptococcus faecalis, Staphylococcus aureus, Candida albicans and Trichophyton mentagrophytes isolates. Furthermore, the flavonol aglycones in the leaves after acid hydrolysis were qualitatively and quantitatively analysed by HPLC, and quercetin was found to be the dominant compound (84 mg/g dw). 2009 Copyright (CC) SCS.


Savikin K.P.,Institute for Medicinal Plants Research | Menkovic N.R.,Institute for Medicinal Plants Research | Zdunic G.M.,Institute for Medicinal Plants Research | Tasic S.R.,Institute for Medicinal Plants Research | And 3 more authors.
Journal of Essential Oil Research | Year: 2010

Light yellowish, pleasant-smelling essential oils were obtained in 0.3%, 1.3%, and 0.8% yields from the aerial parts of S. cuneifolia Ten., M. thymifolia (Scop.) Fritsch., and M. dalmatica Benth, respectively. Thirty-four compounds were identified in the oil of M. dalmatica representing 98.7% of total oil composition, while in the oil of M. thymifolia, 21 were identified representing 78.2% of total oil composition. The major compounds in the oil of M. thymifolia included pulegone (50.4%), piperitenone (10.3%) and piperitenone oxide (4.3%), while the oil of M. dalmatica was rich in pulegone (26.7%), piperitenone (21.8%) and piperitenone oxide (25.4%). Fifty-five compounds were characterized in the oil of S. cuneifolia, representing 92.1% of total oil composition. Linalool (19.9%) and α-pinene (12.3%) were found to be the dominant compounds. Antimicrobial activity was noticed in all examined oils with MIC values ranging from 5.6-54 μg/mL. The most active was the oil of S. cuneifolia, which completely inhibited the growth of mostly all tested microorganisms at the lowest supplied concentration. Glandular trichomes (both peltate and capitate) and nonglandular, uniseriate, simple hairs were distributed on abaxial and adaxial surface of the leaves, calyces and stem in all examined species. © 2010 Allured Business Media.


Stevic T.,Institute for Medicinal Plants Research | Beric T.,University of Belgrade | Savikin K.,Institute for Medicinal Plants Research | Sokovic M.,University of Belgrade | And 3 more authors.
Industrial Crops and Products | Year: 2014

The development of protective products with natural origin as alternatives to synthetic fungicides is currently in the spotlight. Qualitative and quantitative chemical analysis of 16 selected essential oils was determined. Then, antifungal properties determined by in vitro microdilution method against 21 fungi isolated from herbal drugs were evaluated. All tested oils showed some antifungal activity against all fungi used. Savory, thyme and oregano oils, characterized by the presence of phenol such as carvacrol and thymol, and rose oil containing mainly monoterpene alcohols (citronellol and geraniol) proved to be the most effective inhibitor of all fungi tested. Also, combination of particular oils showed reduction of the MIC values when combined, commendatory mixtures for potential application in practice. Moreover, the reduction of the total number of fungi, in situ, using selected essential oils was determined. © 2014 Elsevier B.V.


Djordjevic B.,University of Belgrade | Savikin K.,Institute for Medicinal Plants Research | Zdunic G.,Institute for Medicinal Plants Research | Jankovic T.,Institute for Medicinal Plants Research | And 3 more authors.
Plant Foods for Human Nutrition | Year: 2010

Eleven red currant varieties (Ribes rubrum L.) cultured in Serbia were evaluated for some of their biochemical properties such as total phenolics, anthocyanins, ascorbic acid, invert sugar, soluble solid content, and acidity. The average amount of ascorbic acid varied from 50.5-71.6 mg/100 g FW, while concentration of invert sugars ranged from 6.0%-9.0%. The highest amounts of total phenolics and anthocyanins were detected in variety Redpoll (153.4 mg GAE/100 g FW and 19.3 mg/100 g, respectively). Red currants were processed to juice, and the phenolic and anthocyanin contents changed as a result of processing. Berries and juices were long-term stored at -18 °C and changes in phytochemicals were monitored. In berries, storage caused the decrease of ascorbic acid content up to 49%, and a general reduction of total phenolics was also noticed. In juices, total phenolics content increased after one year of storage. In both berries and juices total anthocyanins increased during storage by up to 85% and 50%, respectively. This study demonstrates that certain varieties, namely Redpoll, Jonkheer and London Market are good source of phytochemicals, retaining the nutritional value during processing and storage. © 2010 Springer Science+Business Media, LLC.


Kardum N.,University of Belgrade | Takic M.,University of Belgrade | Savikin K.,Institute for Medicinal Plants Research | Zec M.,University of Belgrade | And 3 more authors.
Journal of Functional Foods | Year: 2014

The effect of long-term polyphenol-rich chokeberry juice consumption on activities of antioxidant enzymes and membrane lipid status in erythrocytes of 25 healthy women was examined. Percentages of membrane fatty acids, superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities were assessed at a baseline, in the middle and at the end of a 3-month-long consumption period. A significant increase in C22:6n-3, n-3 polyunsaturated fatty acids (PUFAs), total PUFAs and unsaturation index and a significant decrease in monounsaturated fatty acids (MUFAs) and n-6:n-3 ratio were found. Significantly higher SOD and GPX activities were also recorded at the end of the study. Serum lipids and glucose were stabile during the consumption period, while the levels of thiobarbituric acid-reactive substances (TBARS), as serum indicator of lipid peroxidation, were reduced significantly. These results indicate a positive impact of regular chokeberry juice consumption on cellular oxidative damage and suggest its putative role in the protection against oxidative stress. © 2014 Elsevier Ltd.


Jovancevic M.,Center for Continental Fruit growing | Balijagic J.,Center for Continental Fruit growing | Menkovic N.,Institute for Medicinal Plants Research | sAvikin K.,Institute for Medicinal Plants Research | And 3 more authors.
Journal of Medicinal Plants Research | Year: 2011

Wild bilberry (Vaccinium myrtillus L.) is very important natural resource of Montenegro. In our study, bilberries were collected in the summer of 2009, from 11 different localities in mountain region of Montenegro (altitude ranged from 665 to 1700 m a.s.l.). The samples were freezed immediately and stored at -25°C until the analysis were done. The total phenolics were estimated by Folin-Ciocalteu method with slight modifications while the amounts of anthocyanins were analyzed according to the prescription of European Pharmacopoea 6.0. Anthocyanin aglycones were analyzed using high performance liquid chromatography (HPLC) after acid hydrolysis. Total phenolic content was determined in all analyzed samples and it ranged from 3.92 to 5.24 mgGAE/g fw. The amounts of total anthocyanins varied between 0.27 to 0.46%. Among eleven analyzed samples, 10 corresponded to the prescriptions of European Pharmacopoea 6.0. Significant correlation between total phenolics and total anthocyanins was noticed (r = 0.843, P < 0.01). Higher amounts of total phenolics and total anthocyanins were detected in samples harvested from localities more exposed to the sun in comparison with berries grown in shadow. Five cyanidin aglycones (delphinidin, cyanidin, petunidin, peonidin, and malvidin) were quantified after acid hydrolysis, where delphinidin was the most abundant in all samples (0.33 to 0.75 mg/g fw). © 2011 Academic Journals.


Brankovic S.,University of Niš | Kitic D.,University of Niš | Radenkovic M.,University of Niš | Veljkovic S.,University of Niš | And 3 more authors.
Journal of Medicinal Food | Year: 2011

Sideritis raeseri spp. raeseri Boiss. & Heldr., known as "mountain tea," has been widely used in the Mediterranean region as a spice and in folk medicine as a very popular decoction because of its anti-inflammatory, carminative, analgesic, antitussive, stomachic, and antimicrobial properties. The study was aimed to investigate the effects of an ethanol extract of S. raeseri on intestinal activity. Air-dried and powdered aerial parts were extracted with 96% ethanol. The rat ileum preparations were incubated in Tyrode's solution gassed (95% O2/5% CO2) at 37°C. The ethanol extract of S. raeseri (0.03-0.3 mg/mL) relaxed spontaneous contractions in isolated rat ileum, similar to that produced by papaverine. The plant extract in a concentration-dependent manner (0.015-0.15 mg/mL) significantly inhibited the contractile response to acetylcholine (P<.01). Atropine inhibited the response to acetylcholine. A similar relaxation-inducing effect of the S. raeseri extract was observed on the precontracted ileum by histamine and barium chloride. Plant extract (0.03-0.3 mg/mL) significantly shifted the histamine concentration-response curve to the right and down (P<.01). The S. raeseri extract (0.03-0.3 mg/mL) significantly inhibited the contractions induced by barium chloride (P<.01). The results show that the ethanol extract of S. raeseri can produce inhibition of the the spontaneous rat ileum contractions and contractions induced by different spasmogens. These data indicate that S. raeseri acts as a spasmolytic on intestinal smooth muscle, which justifies its use in gastrointestinal disorders. © 2011 Mary Ann Liebert, Inc. and Korean Society of Food Science and Nutrition.

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