Entity

Time filter

Source Type


Korovina O.S.,Institute for Medical Biotechnology | Korneev D.V.,Institute for Medical Biotechnology | Levagina G.M.,Institute for Medical Biotechnology | Bogryantseva M.P.,Institute for Medical Biotechnology | Masycheva V.I.,Institute for Medical Biotechnology
Applied Biochemistry and Microbiology | Year: 2013

A molecular construct of a spherical shape containing double-stranded RNA (dsRNA) in the center, coated by a spermidine-polyglucine coat, and carrying recombinant deltaferon-an analogue of recombinant interferon-gamma-has been designed. It is shown that deltaferon in this construct retains its specific activity at a level of 9 × 104 U/mg. It was found that deltaferon and dsRNA in the construct were characterized by enhanced resistance to proteases (trypsin) and nucleases (E. coli ribonuclease) as compared to free deltaferon and dsRNA. An increased resistance of deltaferon in the construct to environmental temperature during storage was also demonstrated. © 2013 Pleiades Publishing, Inc.


Volosnikova E.A.,Institute for Medical Biotechnology | Akulova N.I.,Institute for Medical Biotechnology | Gogina Y.S.,Institute for Medical Biotechnology | Levagina G.M.,Institute for Medical Biotechnology | And 4 more authors.
Applied Biochemistry and Microbiology | Year: 2010

Optimization of the stage of fractioning of nucleotide material has been performed, and a technology for purification and obtaining of plasmid DNAs for pharmaceutical purposes has been developed. Plasmid DNAs can be used for construction of DNA vaccines to urgent viral infections. Conditions for extraction and preliminary purification of DNA by salt and alcohol precipitations were determined. An effective gel-filtration procedure of separation of plasmid DNA from contaminants was suggested. © 2010 Pleiades Publishing, Ltd.

Discover hidden collaborations