Institute For Lebensmittelqualitat Und Sicherheit

Hannover, Germany

Institute For Lebensmittelqualitat Und Sicherheit

Hannover, Germany
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Wickhorst J.-P.,Justus Liebig University | Hassan A.A.,University of Mosul | Sammra O.,Justus Liebig University | Huber-Schlenstedt R.,Tiergesundheitsdienst Bayern e.V. Eutergesundheitsdienst | And 4 more authors.
Folia Microbiologica | Year: 2016

In the present study, three Arcanobacterium pluranimalium strains isolated from bovine milk samples of three cows of three farms (two cows with subclinical mastitis) could successfully be identified by phenotypical investigations, by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) analysis and genotypically by sequencing the molecular targets 16S rDNA, 16S–23S rDNA intergenic spacer region (ISR), the β subunit of bacterial RNA polymerase encoding gene rpoB, the glyceraldehyde 3-phosphate dehydrogenase encoding gene gap, the elongation factor tu encoding gene tuf, and the pluranimaliumlysin encoding gene pla. The latter could also be identified by a loop-mediated isothermal amplification (LAMP) assay. The presented phenotypic and genotypic approaches might support the identification of A. pluranimalium in future and might help to understand the role this species plays in bovine mastitis. © 2016, Institute of Microbiology, Academy of Sciences of the Czech Republic, v.v.i.


Grabowski N.T.,Institute For Lebensmittelqualitat Und Sicherheit | Ahlfeld B.,Institute For Lebensmittelqualitat Und Sicherheit | Klein G.,Institute For Lebensmittelqualitat Und Sicherheit
Archiv fur Lebensmittelhygiene | Year: 2012

A reliable micro biological analysis of milk samples incl. antibiogram remains fundamental when combating mastitis on herd level. The present paper summarizes the results of mastitis pathogen isolation performed at the "milk laboratory" of the institute between 2004 and 2010. For that, quarter or half foremilk and animal composite milk samples (n = 61.357) were analysed according to NMC recommendations. In case of bacteriologically positive results, antibiograms were made for selected strains (n = 22.859; MIC performed according to CLSI IM31-A3]). Approx. V3 of samples originated from the university's clinics, the rest from local veterinarians and dairy farmers (predominantly Northern Germany). Cows amounted for 96 5 % of samples, sheep and goats for 3.4 %, and equines and zoo animals (Patagonian mara Dolichotis patagonum, night monkeys Aotus spp.) for 0.1 %. More than half of samples (52-57 %) resulted bacteriologically positive. Those samples from cows contained coagulase-negative staphylococci (CNS; 24 %), Streptococcus uberis (13 %), Corynebacterium spp. (12 %), Escherichia coli (12 %), Staphylococcus aureus (9 %), Enterococcus spp. and yeasts (7 % each) as well as other pathogens (17 %). In goats and sheep, CNS (66 %) and S. aureus (19 %) predominated. The amount of cow-associated pathogens decreased in favour of environmental and opportunistic pathogens. The evaluation of antibiograms showed that many of the most frequently isolated pathogens of bovine mastites (except for E. coll) displayed high and stable sensitivity rates (> 95 %) towards several antibiotics (e. g. oxacillin, ampicillin). Resistance towards some other products (e. g. erythromycin, tetracyclines) increased markedly. © M. & H. Schaper GmbH & Co.


Janisch S.,University of Gottingen | Wicke M.,University of Gottingen | Krischek C.,Institute For Lebensmittelqualitat Und Sicherheit
Fleischwirtschaft | Year: 2011

Poultry meat stored in modified atmosphere packages sometimes shows a high variation of the meat colour. Since the colour of the meat varies physiologically, it is necessary to reduce colour differences in the packages. In the present study the influence of gender on the meat quality of M. pectoralis superficialis (MPS) was investigated. Therefore cockerels and hens of the genetic line Ross 308 were considered. The cockerels had significantly higher carcass, breast muscle and leg weights, whereas the breast and thigh yields were similar between the genders. The MPS of the hens had significantly higher drip loss and electrical conductivity values, and lower pH values. Looking at the brightness (L *) and redness (a *) values over 3 days after slaughter, the L * of the meat increase and a * remained nearly unchanged, but this development was independent of the gender of the broilers. The results show that sexing of broiler chicks shortly after hatch might be useful, to reduce the variation of the carcass and meat quality parameters during slaughter and dissection but especially during packaging of the meat.


Grabowski N.T.,Institute For Lebensmittelqualitat Und Sicherheit | Klein G.,Institute For Lebensmittelqualitat Und Sicherheit
Archiv fur Lebensmittelhygiene | Year: 2010

Increased travelling among Europeans, enhanced immigration towards Europe, harmonization of trade legislation, retail shops for Asian and African products and food trade via internet continue to diversify the food supply. Consumers may therefore encounter "exotic" foodstuffs they may not be able to evaluate in terms of food quality and food safety. Corresponding data is scarce and eventually outdated. This paper therefore deals with the microbiological and chemical properties of selected "exotic" foodstuffs for a general survey regarding both diversity and quality of the supply. The selection comprised industrially-processed foods from abroad (milk and meat products, beverage and ice-cream powders), home-made products, fresh bison, moose, reindeer and kangaroo meat and uncommon foodstuffs, i. e. various crustaceans, dried butterfly caterpillars (Cirina forda, "kanni") and condiments made of dried shrimps ("yet") or fruits [Parkia globosa, "soumbalá"). A total of n = 33 samples was purchased regularly and analysed microbiologically and/or chemically. Most samples were analysed readily, but an objective interpretation of the results was frequently difficult due to the lack of (legal) thresholds. Still, elevated bacterial counts (up to 108 cfu/g) including Enterobacteriaceae (fresh meat), Bacillus cereus (cheese of raw ewe's milk) as well as Escherichia coli and salmonellae in cervid and kangaroo meat suggest a health risk for some products. Thus, no uniform picture forms; despite a reduced sample size consuming home-made or raw foods sent via mail clearly poses a greater risk to the consumer's health than industrially crafted products do, regardless of the distribution channel. Retail shops specialized in ethnic foods proved to offer safe products.© M. & H. Schaper GmbH & Co.


Grabowski N.T.,Institute For Lebensmittelqualitat Und Sicherheit | Klein G.,Institute For Lebensmittelqualitat Und Sicherheit | Lopez A.M.,CSIC - Institute of Agricultural Chemistry and Food Technology
Critical Reviews in Food Science and Nutrition | Year: 2013

In Europe, uncommon foodstuff (UFS, i.e., traditional foods from specific European regions and uncommon ethnic foods from non-EU countries) have been contributing to a diversification of the food supply. E-commerce and specialized retail shops are the main sources for UFS. This article discusses the legal bases for UFS introduction and evaluation. By means of 35 representative UFS, this article analyses the possibilities of trade and veterinary inspection of these products in Germany, comparing European Union and national food legislation with the many idiosyncrasies the UFS presents. Conservatory legislation bans the trade with endangered species (primates, cetaceans, songbirds), but for many other species, this is a complex matter that may ban only subpopulations from trade. Although introduction of legal UFS is regulated (yet complicated), the lack of appropriate definitions, intra-European trade harmonization, and of sufficient scientific knowledge hampers a satisfactory evaluation of many UFSs, for example, reptile meat or terrestrial insects. In these cases, official inspection would only be very basic. © 2013 Copyright Taylor and Francis Group, LLC.


Sammra O.,Justus Liebig University | Balbutskaya A.,Justus Liebig University | Nagib S.,Justus Liebig University | Alber J.,Justus Liebig University | And 5 more authors.
Berliner und Munchener Tierarztliche Wochenschrift | Year: 2014

The present study was designed to characterize phenotypically and genotypically an Arcanobacterium haemolyticum strain (A. haemolyticum P646) isolated from a purulent nasal discharge of a donkey. A. haemolyticum P646 showed, compared to sheep blood, an enhanced hemolytic reaction on rabbit blood agar, a synergistic CAMP-like reaction with Streptococcus agalactiae and Rhodococcus equi as indicator strains, a reverse CAMP reaction in the zone of Staphylococcus aureus β-hemolysin and the typical biochemical properties of this species. The species identity could be confirmed by MALDI-TOF MS analysis, by sequencing the 16S rDNA and glyceraldehyde-3-phosphate dehydrogenase encoding gene gap and by amplification of A. haemolyticum specific parts of 16S-23S rDNA intergenic spacer region and 23S rDNA. A. haemolyticum P646 and the reference strain A. haemolyticum DSM 20595 were further characterized by amplification of the putative virulence genes encoding arcanolysin, phospholipase D, hemolysin A, CAMP factor family protein, a collagen binding protein and two neuraminidases which were present for A. haemolyticum DSM 20595. A. haemolyticum P646 showed a comparable gene spectrum but was negative for the genes encoding collagen binding protein and neuraminidase H. To our knowledge, the present study is the first phenotypic and genotypic characterization of an A. haemolyticum strain isolated from a donkey. © 2014 Schlütersche Verlagsgesellschaft mbH & Co. KG.


PubMed | Justus Liebig University, Institute For Lebensmittelqualitat Und Sicherheit, SAC Consulting Veterinary Services, Animal Health and Veterinary Laboratories Agency and RIPAC LABOR GmbH
Type: Journal Article | Journal: Veterinary microbiology | Year: 2014

In the present study 13 Arcanobacterium pluranimalium strains isolated from various animal origin could successfully be identified phenotypically by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) and genotypically by sequencing 16S rDNA and the pluranimaliumlysin encoding gene pla. The detection of mass spectra by MALDI-TOF MS and the novel genotypic approach using gene pla might help to identify A. pluranimalium in future and might elucidate the role this species plays in infections of animals.


PubMed | Institute For Lebensmittelqualitat Und Sicherheit, Justus Liebig University, Tiergesundheitsdienst Bayern e.V. Eutergesundheitsdienst, University of Mosul and Bruker
Type: Journal Article | Journal: Folia microbiologica | Year: 2016

In the present study, three Arcanobacterium pluranimalium strains isolated from bovine milk samples of three cows of three farms (two cows with subclinical mastitis) could successfully be identified by phenotypical investigations, by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) analysis and genotypically by sequencing the molecular targets 16S rDNA, 16S-23S rDNA intergenic spacer region (ISR), the subunit of bacterial RNA polymerase encoding gene rpoB, the glyceraldehyde 3-phosphate dehydrogenase encoding gene gap, the elongation factor tu encoding gene tuf, and the pluranimaliumlysin encoding gene pla. The latter could also be identified by a loop-mediated isothermal amplification (LAMP) assay. The presented phenotypic and genotypic approaches might support the identification of A. pluranimalium in future and might help to understand the role this species plays in bovine mastitis.


PubMed | Justus Liebig University, Institute For Lebensmittelqualitat Und Sicherheit, Frankfurt Zoo and Landesbetrieb Hessisches Landeslabor
Type: | Journal: Journal of veterinary medicine | Year: 2015

The present study was designed to characterize phenotypically and genotypically an Arcanobacterium pluranimalium strain (A. pluranimalium 4868) following necropsy from a juvenile giraffe. The species identity could be confirmed by phenotypical investigations and by MALDI-TOF MS analysis, by sequencing the 16S rDNA, pluranimaliumlysin encoding gene pla, and glyceraldehyde-3-phosphate dehydrogenase encoding gene gap with sequence similarities to A. pluranimalium reference strain DSM 13483(T) of 99.2%, 89.9%, and 99.1%, respectively. To our knowledge, the present study is the first phenotypic and genotypic characterization of an A. pluranimalium strain isolated from a giraffe.


PubMed | Justus Liebig University, Institute For Lebensmittelqualitat Und Sicherheit, Institute For Terrestrische Und Aquatische Wildtierforschung and Bruker
Type: | Journal: Journal of veterinary medicine | Year: 2015

Arcanobacterium phocisimile, a newly described species with the type strain A. phocisimile2698(T) isolated from a vaginal swab of a harbour seal and four additional A. phocisimile strains also isolated from four harbour seals could reliably be identified by phenotypic properties, by matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS), and by sequencing the genomic targets 16S rDNA and 16S-23S rDNA intergenic spacer region and the genes rpoB and gap. The A. phocisimile strains investigated in the present study were isolated together with several other bacterial species indicating that the pathogenic importance of A. phocisimile remains unclear. However, the detection of peptidic spectra by MALDI-TOF MS and the presented phenotypic and genotypic approach might help to identify A. phocisimile in future.

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