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Quadri A.,University of Zurich | Gojanovic B.,La Tour Sport Medicine SOMC. | Gojanovic B.,University of Lausanne | Noack P.,Swiss Olympic Medical Center Abtwil | And 4 more authors.
Schweizerische Zeitschrift fur Sportmedizin und Sporttraumatologie | Year: 2016

Introduction: Vitamin D deficiency is very prevalent in world population and growing evidence shows that also athletes are affected. Vitamin D deficiency causes beside bone disorders, musculoskeletal pain, muscle weakness and is associated with many other health disorders. For athletes in particular it may impair training and performance, prolong recovery and increase risk of injury. We therefore analyzed Vitamin D levels in Swiss athletes focusing on prevalence according to age, gender, seasonal variations, indoor or outdoor sports, sunscreen use and Vitamin D supplementation. Methods: This study was performed in a convenient sample of 655 Swiss Olympic athletes over one year. Blood samples were obtained and a questionnaire was filled in at Swiss Olympic Medical Centers or Bases during an annual routine exam. Data were then sent to the central laboratory of the Aarau hospital where they were processed and sent to us in an anonymized version. Vitamin D levels were categorized into deficiency (< 50 nmol/1), insufficiency (between 50 and 75 nmol/1) and adequate levels (> 75 nmol/1) of 25-hydroxyvi-tamin D. By means of the questionnaire, we assessed age, gender, type of sport, symptoms possibly related to Vitamin D deficiency during the last year, frequency of sunscreen use and Vitamin D-containing medications of the athletes. Results: 13.5% of the participants (total number = 651) presented a Vitamin D deficiency, 37.8% a Vitamin D insufficiency so that more than half of the athletes (51.2%) had inadequate Vitamin D levels. Inadequate Vitamin D levels were more prevalent in younger athletes, during seasons with lower sun exposure, in indoor sports during the sun deprived seasons, in athletes without vitamines supplementation and in athletes of lower Swiss Olympic classes compared to their counterparts. Conclusions: The results of the study show that the prevalence of inadequate Vitamin D levels in Swiss athletes is substantial for younger athletes (< 18 years of age) and for indoor athletes during sun deprived periods of the year. However, a general vitamin supplementation containing Vitamin D reduced the prevalence of Vitamin D inadequacy which is especially relevant during sun deprived seasons. Source

Huber A.R.,Institute For Labormedizin
Therapeutische Umschau | Year: 2015

Outcome studies and health technology assessment and appraisals have become more and more prominent in regard to the utility of laboratory testing in clinical medicine. In the past years many valuable and useful studies have been published that demonstrate an increasing value of laboratory testing in a variety of clinical situations or for a good number of disease diagnosis and monitoring. An excellent outcome using laboratory testing can only be achieved when the tests (methods) have an outstanding performance in terms of sensitivity, specificity, imprecision and robustness. Further, not only has the test per se to be outstanding, it is also of uttermost importance that the clinical setting, in which the test is performed, fits and that the preanalytic requirements are fulfilled as well as that the statistical rules are followed. The laboratory test has to be incorporated into an integrated approach respecting in a weighted attempt as many as possible aspects of health care. © 2015 Verlag Hans Huber Source

Gruber R.,Institute For Labormedizin | Borgmann S.,Klinische Infektiologie und Hygiene
Zeitschrift fur Rheumatologie | Year: 2014

Laboratory diagnostics play a fundamental role in rheumatology but must always be interpreted in the context of symptoms and clinical signs. Laboratory tests have a variety of purposes, such as confirmation or negation of a diagnosis, differential diagnosis, evaluation of activity and prognosis, involvement of organs and drug side effects. Markers of inflammation and specific autoantibodies are the most important laboratory parameters in rheumatology. Thus, with the suspicion of rheumatoid arthritis the analysis of C-reactive protein (CRP), erythrocyte sedimentation rate (ESR), the rheumatoid factor and anti-cyclic citrullinated peptide (anti-CCP or ACPA) should be performed as the first line tests. Only a few antibody titers are suitable for monitoring of disease activity. Some autoantibodies exhibit such a high diagnostic value that the antibodies are included in the classification criteria or in the definition of a disease entity. © 2014 Springer-Verlag. Source

Egerer K.,Charite - Medical University of Berlin | Roggenbuck D.,GA Generic Assays GmbH | Roggenbuck D.,Lausitz University of Applied science | Buttner T.,GA Generic Assays GmbH | And 7 more authors.
Arthritis Research and Therapy | Year: 2011

Introduction: Diagnosis of antiphospholipid syndrome (APS) still remains a laboratory challenge due to the great diversity of antiphospholipid antibodies (aPL) and their significance regarding APS-diagnostic criteria.Methods: A multi-line dot assay (MLDA) employing phosphatidylserine (PS), phosphatidylinositol (PI), cardiolipin (CL), and beta2-glycoprotein I (β2 GPI) was used to detect aPL, immunoglobulin G (IgG) and immunoglobulin M (IgM) in 85 APS patients, 65 disease controls, and 79 blood donors. For comparison, anti-CL and anti-β2 GPI IgG and IgM were detected by enzyme-linked immunosorbent assay (ELISA).Results: The level of agreement of both methods was good for anti-CL IgG, moderate for anti-CL IgM, very good for anti-β2 GPI IgG, and moderate for anti-β2 GPI IgM (kappa = 0.641, 0.507, 0.803 and 0.506, respectively). The frequency of observed discrepancies for anti-CL IgG (1.75%), anti-CL IgM (3.93%), anti-β2 GPI IgG (1.75%), and anti-β2 GPI IgM (0.87%) was low (McNemar test, P < 0.05, not-significant, respectively). Sensitivity, specificity, positive (+LR) and negative (-LR) likelihood ratios for at least one positive aPL antibody assessed by ELISA were 58.8%, 95.8%, 14.1, and 0.4, respectively, and for at least three positive aPl IgM and/or one positive aPL IgG by MLDA were 67.1%, 96.5%, 19.3, and 0.3, respectively. The frequency of IgM to PI, PS and CL, and combination of three or more aPL IgM detected by MLDA was significantly higher in APS patients with cerebral transient ischemia (P < 0.05, respectively).Conclusions: The novel MLDA is a readily available, single-step, sensitive diagnostic tool for the multiplex detection of aPL antibodies in APS and a potential alternative for single aPL antibody testing by ELISA. © 2011 Egerer et al.; licensee BioMed Central Ltd. Source

Steglich M.,Robert Koch Institute | Nitsche A.,Robert Koch Institute | Von Muller L.,Saarland University | Von Muller L.,Institute For Labormedizin | And 6 more authors.
PLoS ONE | Year: 2015

We applied whole-genome sequencing to reconstruct the spatial and temporal dynamics underpinning the expansion of Clostridium difficile ribotype 027 in Germany. Based on resequencing of genomes from 57 clinical C. difficile isolates, which had been collected from hospitalized patients at 36 locations throughout Germany between 1990 and 2012, we demonstrate that C. difficile genomes have accumulated sequence variation sufficiently fast to document the pathogen's spread at a regional scale. We detected both previously described lineages of fluoroquinolone-resistant C. difficile ribotype 027, FQR1 and FQR2. Using Bayesian phylogeographic analyses, we show that fluoroquinolone-resistant C. difficile 027 was imported into Germany at least four times, that it had been widely disseminated across multiple federal states even before the first outbreak was noted in 2007, and that it has continued to spread since. © 2015 Steglich et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Source

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