Iii INCT Institute for Investigation in Immunology

São Paulo, Brazil

Iii INCT Institute for Investigation in Immunology

São Paulo, Brazil
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Castro L.M.,Biomedical Science Institute | Castro L.M.,Federal University of São Paulo | Berti D.A.,Biomedical Science Institute | Russo L.C.,Biomedical Science Institute | And 5 more authors.
AAPS Journal | Year: 2010

Cells produce and use peptides in distinctive ways. In the present report, using isotope labeling plus semi-quantitative mass spectrometry, we evaluated the intracellular peptide profile of TAP1/β2m-/- (transporter associated with antigen-processing 1/2 microglobulin) double-knockout mice and compared it with that of C57BL/6 wild-type animals. Overall, 92 distinctive peptides were identified, and most were shown to have a similar concentration in both mouse strains. However, some peptides showed a modest increase or decrease (~2-fold), whereas a glycine-rich peptide derived from the C-terminal of neurogranin (KGPGPGGPGGAGGARGGAGGGPSGD) showed a substantial increase (6-fold) in TAP1/β2m-/- mice. Thus, TAP1 and β2microglobulin have a small influence on the peptide profile of neuronal tissue, suggesting that the presence of peptides derived from intracellular proteins in neuronal tissue is not associated with antigens of the class I major histocompatibility complex. Therefore, it is possible that these intracellular peptides play a physiological role. © 2010 American Association of Pharmaceutical Scientists.

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