Huang S.,Peking Union Medical College |
Lyu Y.,Institute for Infectious Disease and Endemic Disease Prevention and Control |
Qing X.,Qufu Normal University |
Wang W.,Qufu Normal University |
And 3 more authors.
Biotechnology Letters | Year: 2013
A gene encoding the 3BC of human enterovirus 71 (EV71) was cloned and inserted into a derivative of plasmid pET-32a(+) driven by T7 promoter. The expressed 3C protease (3Cpro) autocatalytically cleaved itself from the recombinant protein Trx-3BC and the mature 3Cpro partitioned in the soluble fraction of bacterial lysate. The 13-amino-acid peptide substrates with the junction of 3B/3C were used to verify the proteolysis activity of the purified 3Cpro. The EV71 3Cpro had a Km value of 63 μM (measured by a continuous fluorescence assay). The other solid-phase activity assay of the EV71 3Cpro was developed using HPLC to analyze the proteolytic products. The combination of two activity assays contributes to promote the identification of the specific inhibitors targeted to the EV71 3Cpro. © 2013 Springer Science+Business Media Dordrecht.
Li Y.,Institute for Infectious Disease and Endemic Disease Prevention and Control |
Quanyi W.,Institute for Infectious Disease and Endemic Disease Prevention and Control |
Li X.,Institute for Infectious Disease and Endemic Disease Prevention and Control |
He Z.,Institute for Infectious Disease and Endemic Disease Prevention and Control |
And 4 more authors.
Chinese Journal of Endemiology | Year: 2014
Objective To investigate the nutritional status among residents and salt iodine content in Beijing, and to provide a basis for iodine deficiency disorders (IDD) prevention and control.Methods A cross-sectional investigation of 120 families from Chaoyang District and Daxing District in Beijing was carried out in 2012. Twenty-four hours urine samples were collected from 360 family members to test urine iodine level. Household salt samples were collected and detected of salt iodine ; Iodine nutrition per capita intake and daily family salt consumption were investigated to calculate the contribution rate of iodine by salt iodine.Results The median of urinary iodine concentration in children aged 8 - 10, adult females and males were 165.40, 138.85 and 155.65 μg/L, respectively. Iodine nutrition per capita intake for children aged 8-10, adult females and males were 263.54, 286.68 and 328.02 μg/d, respectively. Salt iodine supply contributed 84.68% of human iodine nutrition intake among residents in Beijing. Conclusions Per capita iodine nutrition intake among residents in Beijing has reached the recommended nutrient intake. Iodine in salt is the main source of dietary intake of iodine.