Institute for Fruit Growing

Fruit, Belarus

Institute for Fruit Growing

Fruit, Belarus
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Cieslinska M.,Research Institute of Horticulture | Valasevich N.,Institute for Fruit Growing
Journal of Plant Diseases and Protection | Year: 2016

Apple mosaic virus (ApMV) infects over 65 plants species of Rosaceae family including hazelnut. The virus causes chlorotic or yellow patterns, rings and mosaic symptoms on the leaves and losses in yield of nuts. The occurrence and genetic diversity of ApMV were evaluated in 14 plantations of hazelnut in Poland. A total of 13 of 125 hazelnut samples tested positive for ApMV using ELISA and reverse transcriptase-polymerase chain reaction. Several primer pairs were used for RT-PCR to amplify the coat protein (CP) and movement protein (MP) genes as well as complete RNA-3 of the ApMV. Genetic diversity was observed in full-length sequences of RNA-3 of the ApMV isolates from hazelnut. The similarity of nucleotide sequence of the CP and MP genes of the isolates was 90.3-99.9, and 87-99.7 %, respectively. Phylogenetic analysis showed that ApMV isolates from hazelnut grouped separately from the virus strains found in other plant species. © The Author(s) 2016.


Valasevich N.,Julius Kuhn Institute | Valasevich N.,Institute for Fruit Growing | Schneider B.,Julius Kuhn Institute
Journal of Plant Pathology | Year: 2016

The first survey on the occurrence of ‘Candidatus Phytoplasma prunorum’,the etiological agent of European stone fruit yellows (ESFY),on stone fruit trees was conducted in Belarus in autumn 2014 when apricot trees in the experimental field of the Institute for Fruit Growing in Samochvalovichi showed leaf yellowing and rolling with early leaf shedding. In one of the six symptomatic apricot trees examined,phytoplasmas were detected using an universal 16S rDNA based PCR assay. Apricot is not common in Belarus and the origin of infection was unclear. A potential source of local ‘Ca. P. prunorum’ strains is blackthorn (Prunus spinosa),which is an asymptomatic natural host for the pathogen. Roots from three randomly selected blackthorn trees growing next to the apricot orchard were tested and two of them were found to be infected. Phytoplasma species were identified by sequence analysis of the 16S rDNA amplicons which confirmed that apricot and blackthorn trees were infected by ‘Ca. P. prunorum’. To further characterize the Belarusian ‘Ca. P. prunorum’ accessions for strain diversity their imp and hflB genes were examined. This is the first report on the occurrence and molecular diversity of ‘Ca. P. prunorum’ in apricot and blackthorn plants in Belarus. © 2016,Edizioni ETS. All rights reserved.


Valasevich N.,Institute for Fruit Growing | Cieslinska M.,Research Institute of Horticulture | Kolbanova E.,Institute for Fruit Growing
European Journal of Plant Pathology | Year: 2015

Apple mosaic virus (ApMV) is one of the important viruses of apple and rose. Although the virus occurs worldwide the information on the virus presence in Poland and Belarus is limited. Genetic diversity of ApMV isolates from apple originated from Poland and Belarus as well as rose isolates from Poland was investigated based on phylogenetic analyses of movement protein (MP) and coat protein (CP) genes. Comparison of CP gene sequences showed the high sequence identity of the studied ApMV isolates and their clustering with apple and pear strains of ApMV deposited in the GenBank database. There was no correlation between the geographical origin and CP gene sequence variability of Polish and Belarusian ApMV isolates. © 2014, Koninklijke Nederlandse Planteziektenkundige Vereniging.


Urbanovich O.Y.,National Academy of Sciences of Belarus | Kazlouvskaya Z.A.,Institute for Fruit Growing | Yakimovich O.A.,Institute for Fruit Growing | Kartel N.A.,National Academy of Sciences of Belarus
Russian Journal of Genetics | Year: 2011

Using SSR markers designed for Malus × domestica Borkh. genetic polymorphism of 43 pear accessions cultivated in Belarus was examined. A total of 217 alleles were identified with the mean number of 12.8 alleles per marker. The mean PIC value was 0.81; the mean number of informative alleles, 6.49. The heterozygosity level ranged from 0.30 to 0.84. Genetic diversity of SSR alleles in pear and apple genomes was compared. A method of identification of commercial pear cultivars using a set of six SSR markers was suggested. © 2011 Pleiades Publishing, Ltd.


Samus V.,Institute for Fruit Growing | Ozcan M.M.,Selcuk University | Harmankaya M.,Selcuk University | Ozkalp B.,Selcuk University | Al Juhaimi F.,King Saud University
Journal of Food Safety and Food Quality | Year: 2016

Mineral contents of several fruits growing in Minsk in Belarus were determined by Inductively Coupled Plasma Atomic Emission Spectrometry (ICP-AES). The highest K (19141 mg/kg) was found in redberry sample, followed by red plum (big) (13352 mg/Kg), strawberry (13287 mg/Kg), blackberry (12896 mg/kg), yellow grape (12215 mg/kg), yellow plum H1 (12438 mg/kg), yellow plum H2 (12124 mg/kg), black grape (big) (11472 mg/kg), black grape (small) (11692 mg/kg), red plum (small) (10743 mg/Kg) and grape (red) (10572 mg/kg). P contents of fruits changed between 394 mg/kg wild apple) and 3422 mg/kg (redberry). Cr, Mo, Ni and Pb were found in very low concentrations. Generally, while Cu contents of fruits change between 1.6 mg/Kg (red hybrit apple) and 8 mg/kg (red berry), Fe contents ranged from 12.5 mg/kg (yellow pear) to 51.4 mg/kg (black berry). It appears that fruits could serve as good source of some nutrients. © M. & H. Schaper GmbH & Co.


Krivorot A.M.,Institute for Fruit Growing | Martsynkevich D.I.,Institute for Fruit Growing
Acta Horticulturae | Year: 2010

The research was carried out at the Storage and Processing Department of the Institute for Fruit Growing of the National Academy of Sciences of Belarus during the period from 2001 till 2004. The influence of afterharvest treatment of fruits with different kinds of wax-like substances on keeping characteristics of apples (Malus ×domestica) of Belarusian cultivars was studied. Extracts of reed peat, Phragmites communis and?otentilla palustris were used as wax-like substances; and untreated fruits were used as control in the experiment. Treating fruits with reed peat, Phragmites and Potentilla extracts has a positive influence on fruit keeping characteristics in cold storage. It reduces natural loss of mass, increases commodity output, decreases the spread of physiological disorders and microbiological diseases of apples. According to apple fruits keeping characteristics during long-time storage reed peat and Phragmites extracts have the highest effect.?otentilla extract yields a bit to the above mentioned extracts. The apple cultivar factor has more influence on the change of shelf life characteristics in comparison with the extract processing factor.


PubMed | Institute for Fruit Growing
Type: Journal Article | Journal: Archives of virology | Year: 2011

The complete coding sequences were determined for RNA-1 and RNA-2 of five raspberry isolates of Raspberry bushy dwarf virus (RBDV) from Belarus (BY1, BY3, BY8, BY22) and Sweden (SE3). The analysed sequences for both RNA-1 and RNA-2 were highly conserved among these isolates. Phylogenetic analyses including available sequences for the CP gene and the MP gene showed that all analysed RBDV isolates from raspberry were closely related. However, there was no strong correlation between the grouping of raspberry isolates in the phylogenetic analyses and their geographical location. In contrast, RBDV isolates showed a host-dependent relationship with isolates from raspberry and grapevine, forming two distinct clades.

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