National Institute for Forestry
National Institute for Forestry
Dimas-Gonzalez J.,National Institute of Genomic Medicine |
Maldonado-Lagunas V.,National Institute of Genomic Medicine |
Diaz-Chavez J.,National Cancer Institute |
Lopez-Arellano M.E.,National Institute for Forestry |
And 3 more authors.
Oncology Reports | Year: 2017
Breast cancer (BC) is a disease with different clinical, histological and molecular characteristics, frequently presenting mutated tumour-suppressing genes and oncogenes. P53 is a known tumour suppressor that is often mutated in BC; several mutations in p53 inhibit its role as a transcriptional repressor of several oncogenes. Topoisomerase 2a (TOP2a) is a gene target of p53, and it is also a known target for anthracyclines. The aim of the present study, was to analyse the genetic alterations of p53 and TOP2a genes and their levels of protein expression, as well as their association with survival in Mexican women with BC. A total of 102 biopsies were collected (tumour and adjacent tissues) from patients with BC. To identify point mutations and deletions in the p53 gene, the Sanger sequencing method was carried out. Deletions or amplifications for TOP2a gene were determined using quantitative polymerase chain reaction (qPCR). In addition, the expression of the TOP2a and p53 proteins was evaluated by western blotting. Furthermore, p53 protein expression was analysed by proximity ligation assay (PLA)-qPCR. Only 28.5% of the patients were found to have triple-negative breast cancer (TNBC); the average age at the time of diagnosis of these patients was 50 years, and Scarff-Bloom-Richardson (SBR) histological grade III (p=0.0089). No differences in point mutations or deletions in p53, and deletions or amplifications as well as protein expression level of TOP2a were observed between patients with TNBC and non-TNBC patients. However, patients with TNBC showed p53 protein overexpression as determined by PLA-qPCR and western blotting (P<0.0001). Furthermore, we found an association between TOP2a amplification and over-expression of its protein in patients with TNBC (P<0.0001). Concerning p53, overexpression resulted in a lower survival in patients with BC.
Aranda-Gonzalez I.,Autonomous University of Yucatán |
Betancur-Ancona D.,Autonomous University of Yucatán |
Chel-Guerrero L.,Autonomous University of Yucatán |
Moguel-Ordonez Y.,National Institute for Forestry
International Agrophysics | Year: 2017
Drying techniques can modify the composition of certain plant compounds. Therefore, the aim of the study was to assess the effect of different drying methods on steviol glycosides in Stevia rebaudiana Bertoni leaves. Four different drying methods were applied to Stevia rebaudiana Bertoni leaves, which were then subjected to aqueous extraction. Radiation or convection drying was performed in stoves at 60°C, whereas shade or sun drying methods were applied at 29.7°C and 70% of relative humidity. Stevioside, rebaudioside A, rebaudioside B, rebaudioside C, rebaudioside D, dulcoside A, and steviolbioside were quantified by a validated HPLC method. Among steviol glycosides, the content (g 100 g-1 dry basis) of stevioside, rebaudioside A, rebaudioside B, and rebaudioside C varied according to the drying method. The total glycoside content was higher in sun-dried samples, with no significant differences compared to shade or convection drying, whereas radiation drying adversely affected the content of rebaudioside A and rebaudioside C (p <0.01) and was therefore a method lowering total glycoside content. The effect of the different drying methods was also reflected in the proportion of the sweetener profile. Convection drying could be suitable for modern food processing industries while shadow or sun drying may be a low-cost alternative for farmers. © 2017 Irma Aranda-González et al., published by De Gruyter Open.
Gonzalez-Godinez A.,National Institute for Forestry |
Urrutia-Morales J.,National Institute for Forestry |
Gamez-Vazquez H.G.,National Institute for Forestry
Tropical and Subtropical Agroecosystems | Year: 2014
This study compared the ability of breeds Dorper and Katahdin to reproduce out of season. The ewes were kept in confinement conditions and fed with a whole diet (23.4% DM, 12.7% of CP and 2.3 Mcal ME). The sheep were exposed to males of their respective breeds in March-April (217 Dorper and 72 Katahdin) and in May-June (103 Dorper and 37 Katahdin). The proportion of ewe lambed was higher (P < 0.0001) in two breeds bred in March-April (67.7 and 72.2%) than May-June (38.8 and 29.7%), but prolificacy was similar (P > 0.05) in both breeds in the two breeding periods (1.35, 1.35, 1.15 and 1.36 lambs born/ewe lambed in the March-April and May-June breeding, in the Dorper and Katahdin breeds, respectively). It is concluded that ewes of hair Dorper and Katahdin sheep are able to reproduce outside of the reproductive season. However, reproductive efficiency was increased in ewes bred at the beginning than those bred at the end of spring season.
Salgado-Miranda C.,University of Central Mexico |
Loza-Rubio E.,National Institute for Forestry |
Rojas-Anaya E.,National Institute for Forestry |
Garcia-Espinosa G.,National Autonomous University of Mexico
Expert Review of Vaccines | Year: 2013
Since 1970, aquaculture production has grown. In 2010, it had an annual average rate of 6.3% with 59.9 million tons of product and soon could exceed capture fisheries as a source of fishery products. However, the occurrence of viral diseases continues to be a significant limiting factor and its control is important for the development of this sector. In aquaculture farms, fish are reared under intensive culture conditions, and the use of viral vaccines has enabled an increase in production. Several types of vaccines and strategies of vaccination have been developed; however, this approach has not reached the expected goals in the most susceptible stage (fingerlings). Currently, there are inactivated and recombinant commercial vaccines, mainly for salmonids and cyprinids. In addition, updated genomic and proteomic technology has expedited the research and expansion of new vaccine models, such as those comprised of subunits or DNA. The objective of this review is to cover the various types of viral vaccines that have been developed and are available for bony fishes, as well as the advantages and challenges that DNA vaccines present for massive administration in a growing aquaculture, possible risks for the environment, the controversy regarding genetically modified organisms and possible acceptance by consumers. © 2013 2013 Expert Reviews Ltd.
Sanchez-Cohen I.,National Water Research Institute |
Diaz-Padilla G.,National Institute for Forestry |
Velasquez-Valle M.,National Water Research Institute |
Slack D.C.,University of Arizona |
And 2 more authors.
Computers and Electronics in Agriculture | Year: 2015
Rural inhabitants of arid lands constantly face a lack of sufficient water to fulfill their agricultural and household needs. In this situation they have to take quick and precise decisions about how to cope with the situation. Moreover, there is not readily available technical information to support their decisions regarding the course of action they should follow to handle the agro-climatic risk. In this paper a computer model (soil water balance model) is described to assess the impact on crops yields of rainfall shortages in dry lands in Mexico. The model is linked to a knowledge based database where a farmer may find readily available information to support cropping decisions. The knowledge base activates when the computed average crop yield is less than the 50% of the expected crop yield. The knowledge base provides information on risk, potential crops, and the geographical location (counties) where the crop may succeed. Also, it provides a technology to increase water productivity under limited availability situations. Further, the model can evaluate the impact of a climate change scenario (IPCC B2). Other inputs to the model being equal, the user may shift the model to run the climate change scenario and to compare the outputs of the model to assess the climate change impact on future crops yields. © 2015 Elsevier B.V.
Morales A.L.,Autonomous University of Queretaro |
Guzman-Maldonado S.H.,National Institute for Forestry |
Herrera-Hernandez M.G.,National Institute for Forestry
Acta Horticulturae | Year: 2014
Industries discard the pulp (endocarp), and peel (exocarp) of xoconostle or prickly pear fruit cultivar 'Cuaresmeño' (Opuntia matudae) as waste and utilize the flesh (mesocarp) which is the edible portion. Soluble phenols, vitamin C and betalain content of the different structures from three production sites in Mexico was determined as well as the characterization of simple phenols and antioxidant capacity. Soluble phenols of peel (840-863 mg GAE/100 g, DWB) were almost similar to that of flesh (919-986 mg GAE/100 g, dry weigh basis); meanwhile, ascorbic acid concentration of flesh was 2-fold higher compared to that of peel. The phenolic fraction of xoconostle structures consisted of gallic, vanillic, and 4-hydroxybenzoic acids; catechin, epicatechin, and vanillin were also identified by high-performance liquid chromatography - didoe array detection (HPLC-DAD). Xoconostle peel showed higher antioxidant activity compared to that of flesh (2-fold) and pulp (6-fold) and commonly consumed fruits and vegetables. There is potential to use xoconostle peel and pulp for the production of feed or food products.
Guzman-Maldonado S.H.,National Institute for Forestry |
Guzman-Tovar I.,National Institute for Forestry |
Hernandez-Lopez D.,Celaya Institute of Technology
Acta Horticulturae | Year: 2014
The effect of sun-drying and oven-drying on the content of antioxidant compounds and antioxidant capacity of berrycactus fruit was evaluated. The sun drying temperature averaged 45°C while the temperature of the oven was 55°C. It takes only 14 h to produce berrycactus raisins in contrast to the 4 weeks that takes the production of grape raisins. Both the sun and stove drying processes reduced from 70 to 95% the betalains, soluble phenolics, condensed tannins, and ascorbic acid contents after 16 h of treatment. Berrycactus raisins showed similar contents of soluble phenols and condensed tannins when compared to that of blueberry and grape raisins. On the contrary, berrycactus showed 50% lower content of ascorbic acid compared to that of commercial raisins. The fiber contents of berrycactus raisins was 6-fold and 5-fold higher than the content of blueberry and grape raisins, respectively. The antioxidant capacity of berrycactus raisins was similar to that of blueberry raisins and 33% higher when compared to that of grape raisins.
Mejia-Teniente L.,Autonomous University of Queretaro |
Duran-Flores F.D.,Autonomous University of Queretaro |
Chapa-Oliver A.M.,Autonomous University of Queretaro |
Torres-Pacheco I.,Autonomous University of Queretaro |
And 4 more authors.
International Journal of Molecular Sciences | Year: 2013
Hydrogen peroxide (H2O2) is an important ROS molecule (Reactive oxygen species) that serves as a signal of oxidative stress and activation of signaling cascades as a result of the early response of the plant to biotic stress. This response can also be generated with the application of elicitors, stable molecules that induce the activation of transduction cascades and hormonal pathways, which trigger induced resistance to environmental stress. In this work, we evaluated the endogenous H2O2 production caused by salicylic acid (SA), chitosan (QN), and H2O2 elicitors in Capsicum annuum L. Hydrogen peroxide production after elicitation, catalase (CAT) and phenylalanine ammonia lyase (PAL) activities, as well as gene expression analysis of cat1, pal, and pathogenesis-related protein 1 (pr1) were determined. Our results displayed that 6.7 and 10 mM SA concentrations, and, 14 and 18 mM H2O2 concentrations, induced an endogenous H2O2 and gene expression. QN treatments induced the same responses in lesser proportion than the other two elicitors. Endogenous H2O2 production monitored during several days, showed results that could be an indicator for determining application opportunity uses in agriculture for maintaining plant alert systems against a stress. © 2013 by the authors; licensee MDPI, Basel, Switzerland.
Salgado-Hernandez E.G.,National Autonomous University of Mexico |
Bouda J.,National Autonomous University of Mexico |
Villa-Godoy A.,National Autonomous University of Mexico |
Romano-Munoz J.L.,National Institute for Forestry |
And 2 more authors.
Czech Journal of Animal Science | Year: 2014
Concentrations of calcidiol, calcitriol, and minerals in blood serum and colostrum of 14 primiparous and 16 multiparous Holstein dairy cows during short-period prepartum and postpartum were determined and compared. Blood samples were collected between days 5 and 2 prepartum and 6 h, 12 h, 7 and 21 days postpartum. Nearly 66% of primiparous and 71% of multiparous cows had subclinical postpartum hypocalcemia. Prepartum serum calcium (Ca) and inorganic phosphorus (P) were higher in primiparous cows; Ca decreased in both groups at 6 and 12 h and returned to baseline values 7 days postpartum. Calcidiol and calcitriol concentrations were equal on day 5 prepartum in both groups. In multiparous cows, calcidiol and calcitriol concentration increased at 6 h postpartum and remained elevated at 12 h postpartum; there were no changes in primiparous cows for these analytes. The total secretion of Ca in the colostrum from the first milking was similar in both groups and positively correlated with serum Ca at 6 and 12 h after calving. It is concluded that postpartum increases in the calcidiol and calcitriol concentration were a normal response to the decrease of serum calcium concentration only in multiparous cows. The total Ca secretion in the colostrum of the first milking postpartum does not reflect the grade of hypocalcemia.
Vargas-Hernandez M.,Autonomous University of Queretaro |
Munguia-Fragozo P.V.,Autonomous University of Queretaro |
Cruz-Hernandez A.,Autonomous University of Queretaro |
Guerrero B.Z.,National Institute for Forestry |
And 7 more authors.
Industrial Crops and Products | Year: 2014
Methanolic extracts of Acaciella angustissima pods (MEA) were analyzed in order to determine some biological activities such as antioxidant, antimutagenic and antimicrobial properties by 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2'-azinobis-3 ethylbenzothiazoline-6-sulfonic acid (ABTS), ferric reducing antioxidant power (FRAP), Kado microsuspension assay and in vitro antimicrobial assays, respectively. Moreover, a transcriptomic study of Timbe grown in greenhouse conditions was also performed using subtractive suppression hybridization methodology (SSH). MEA presented significant antioxidant activity by DPPH, ABTS and FRAP methods. The maximum concentration of MEA inhibited 80.33% and 77.39% of mutagenicity induced either by 4-nitro-O-phenylenediamine or sodium azide, respectively. MEA presented fungistatic activity against Fusarium oxysporum, Rhizoctonia solani and Phytophtora capsici. Moreover, a good in vivo protection against P. capsici was obtained in comparison to a commercial antifungal used toward this pathogen. Transcriptomic study displayed the expression of genes that have biological importance, such as protein TIC55, GATA transcription factor, maturase-like protein, and NAC domain protein. NAC domain protein might have some biotechnological or industrial application, because, NAC transcription factor plays an important role in responses to biotic and abiotic stress from some plants. Results suggest that Timbe might be useful in pharmaceutical, food and agro-industrial sectors. © 2013 Elsevier B.V.