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Kruševac, Serbia

Peseva V.,University of Prishtina | Stoyanov I.Y.,Plovdiv University | Andjelkovic B.,Institute for Forage Crops | Mladenovic M.,University of Belgrade | And 2 more authors.
Acta Zoologica Bulgarica | Year: 2015

The genetic variability of selectively reared honey bees Apis mellifera from Kosovo was studied within and among populations. We used six alloenzymic systems (MDH-1, ME, EST-3, ACP, PGM and HK), which corresponded to six loci. Five of the studied enzyme systems. i.e. MDH 1, ACP, EST, PGM and HK, manifested intra- And inter-population polymorphism with two or three alleles. ME locus was monomorphic in all of the tested honey bee populations. The observed and expected heterozygosities ranged from 0.104 to 0.164 and from 0.093 to 0.130, respectively. The percentage of polymorphic loci among the studied honey bee populations from Kosovo was between 16.7% and 50%. The calculated NEI's genetic distance ranged between 0.001 and 0.019. The estimated mean FST value from allozyme data was 0.028.

Andjelkovic S.,Institute for Forage Crops
Journal of Food, Agriculture and Environment | Year: 2012

Different plant organs (leaves, flowers, stems or roots) from naturally occurring wild plants and trees, and cultivated plants (vegetables and fruits) were evaluated as possible bioindicators of heavy-metal pollution in the Republic of Serbia. Concentrations of Pb, Zn and Cd were determined in plant parts collected from areas with different degrees of metal pollution (Kosovska Mitrovica - industrial area and Leposavić - control area). Accumulation and distribution of heavy metals in the plant depend on the plant species, the levels of the metals in the soil and air, the element species and bioavailiability, pH, cation exchange capacity, climacteric condition, vegetation period and multiple other factors. The highest amounts of heavy metals were found in the root of the sensitive Plantago major (Pb 660, Zn 2300 and Cd 33.25 μg g -1 d.w.) and less in the resistant Rumex acetosella. In over ground organs the highest amounts of heavy metals were found in the leaves (283 μg g -1 d.w. Pb) and then in the fruits (3,5-136 μg g -1) and vegetables (1.5-13 μg g -1). In this study, the bark of Robimia pseudoacacia was a better bioindicator of heavy-metal pollution than other plant parts. Summarizing the results, it can be concluded that R. pseudoacacia, P. major and R. acetosella were better metal accumulators and fruits and vegetables were metal avoiders.

Popovic V.,Serbian Institute of Field and Vegetable Crops | Sokolovic D.,Institute for Forage Crops
Genetika | Year: 2011

This research studied the phenotypic correlation coefficients between three Sorghum species, namely forage sorghum S. bicolor Moench. (c. NS-Džin), Sudan grass S. sudanense L. (c. Zora) and interspecies hybrid S. bicolor × S. sudanense (c. Siloking). The analyses were performed on plant material samples taken from the first cutting, when plants were in the beginning phase of tasseling. The following morphologic traits were studied: plant height, number of leaves per plant, stem leaf weight and mean stem weight. Additionally, their direct and indirect effect on dependent variable green biomass yield was analysed, for which path coefficients were calculated. This method enables more quality and full insight into relations existing among the studied traits, more precise establishment of cause-effect connections among them, as well as to separate direct from indirect effects of any particular trait on dependent variable, being biomass yield in this case. The analysis of phenotypic coefficients revealed differences in direct and indirect effect of certain traits on dependent variable. Sudan grass had the highest stem (2.281 m) and most leaves per plant (7.917). Forage sorghum had the largest leaf weight per plant (49.05 g), while interspecies hybrid had the highest mean stem weight (80.798 g). Variations of these morphologic traits among species were found to be significant and very significant. Morphologic traits - stem height and weight significantly affected sorghum green biomass yield. Leaf number and leaf portion in total biomass were negatively correlated with yield. Cultivars differed significantly regarding morphologic and productive traits. Sudan grass had the lowest green biomass yield, while forage sorghum and interspecies hybrid had significant yield increase.

Krnjaja V.,Serbian Institute for Animal Husbandry | Mandic V.,Serbian Institute for Animal Husbandry | Levic J.,Maize Research Institute Zemun Polje | Stankovic S.,Maize Research Institute Zemun Polje | And 3 more authors.
Crop Protection | Year: 2015

In this study, the influence of nitrogen (N)-fertilization on Fusarium head blight (FHB) intensity (disease index), spike weight (SW), grain weight per spike (GWS), fungal incidence and deoxynivalenol (DON) and zearalenone (ZON) contamination was evaluated in winter wheat after artificial inoculation with Fusarium graminearum under field conditions. The trial was conducted using two Serbian wheat cultivars (Pobeda and Renesansa) during two growing seasons (2010-2011 and 2011-2012). Three nitrogen rates, 0, 75 and 150kgNha-1 were evaluated.Nitrogen at higher rates (75 and 150kgha-1) significantly increased SW and GWS in 2011 but there was no significant impact on FHB index in both growing seasons. Cultivars did not differ significantly in SW and GWS; however, FHB index was significantly higher (P=0.05) in Renesansa in 2012. The effect of N rates was not significant on incidence of F. graminearum and DON and ZON levels in wheat kernels in 2011, but it was significant (P≤0.01) in 2012 in which the highest level of incidence of F.graminearum and mycotoxins was observed in the treatment with 150kgNha-1. In both growing seasons, the effects of cultivar and spike treatments were significant (P≤0.01) for DON, whereas these effects were not significant for ZON in 2011. Higher levels of DON were observed in Pobeda, whereas Renesansa had higher levels of ZON. Furthermore, there were higher levels of DON and ZON in inoculated than non-inoculated spikes in both growing seasons.These results indicated that nitrogen fertilization did not increase FHB intensity in both growing seasons and did not increase levels of DON and ZON in 2011. On the other hand, artificial inoculation significantly increased FHB index in both growing seasons, resulting in greater contamination of kernels with F.graminearum and mycotoxins, in particular DON. Although the two cultivars differed in their effects on some of the investigated parameters, both were susceptible to FHB and mycotoxin accumulation, in particular DON. © 2014 Elsevier Ltd.

Vasic T.,Institute for Forage Crops | Krnjaja V.,Serbian Institute for Animal Husbandry | Jevremovic D.,Fruit Research Institute | Stankovic S.,Maize Research Institute Zemun Polje | And 3 more authors.
Genetika | Year: 2016

A total of 17 isolates of Colletotrichum from alfalfa (Medicago sativa L.) and red clover (Trifolium pratense L.) plants with anthracnose symptoms were collected from 11 districts in Serbia during 2005-2010 and tested for variability in vegetative compatibility groups (VCGs) and restriction fragment length polymorphisms (RFLP). Nitrate nonutilising (nit) mutants were isolated from each of investigated C. destructivum isolates by selecting chlorate-resistant sectors on medium with chlorate. The isolates were grouped in five VCGs while one isolate was self-incompatible. No relationship was found between VCGs and geographical origin of the isolates. Restriction Fragment Length Polymorphism (RFLP) analysis of a 900 bp intron of the glutamine synthetase (GS) gene revealed a unique polymorphic profile of C. destructivum isolates, distinct from the profiles of other Colletotrichum species. An identical profile was produced for all C. destructivum isolates, regardless of their host and geographical origin. PCR-RFLP failed to detect some the Serbian C. destructivum isolates.

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