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Scatassa M.L.,Institute For Experimental Veterinary Medicine Of Sicily A Mirri | Cardamone C.,Institute For Experimental Veterinary Medicine Of Sicily A Mirri | Miraglia V.,Institute For Experimental Veterinary Medicine Of Sicily A Mirri | Lazzara F.,Institute For Experimental Veterinary Medicine Of Sicily A Mirri | And 5 more authors.
Italian Journal of Food Safety | Year: 2015

Traditional Sicilian cheese productions are carried out employing traditional wooden vats, called tina. Many studies have highlighted the beneficial role of wooden dairy equipment by contributing to enriching the milk microflora and improving the acidification processes. The present work was undertaken to evaluate the safety of the wooden vats used to coagulate milk. To this purpose, the different microbial populations hosted onto the internal surfaces of the vats used to produce two different stretched cheeses, namely Caciocavallo Palermitano and Vastedda della valle del Belìce DOP, were investigated for the presence of spoilage and pathogenic microorganisms as well as for bacteria with inhibitory effect in vitro against pathogenic microorganisms. A wide biodiversity of protechnological lactic acid bacteria (LAB), in terms of species, was revealed. Several LAB inhibited the growth of Listeria monocytogenes ATCC 7644. The wooden vats analysed resulted safe for three main findings: absence of the main pathogenic species, presence of high levels of LAB, anti-Listeria activity of many LAB. © Copyright M.L. Scatassa et al., 2015. Source


Ferrantelli V.,Institute For Experimental Veterinary Medicine Of Sicily A Mirri | Costa A.,Institute For Experimental Veterinary Medicine Of Sicily A Mirri | Graci S.,Institute For Experimental Veterinary Medicine Of Sicily A Mirri | Buscemi M.D.,Institute For Experimental Veterinary Medicine Of Sicily A Mirri | And 4 more authors.
Italian Journal of Food Safety | Year: 2015

In this work a total of 949 fish samples were analysed for the identification of nematode larvae belonging to the Anisakidae family. Biomolecular application for the identification of Anisakidae larvae can be an optimal instrument for the traceability of fish products, described on the Reg. EC 178/2002. Results confirm a correlation between geographical distribution of fishes and presence of specific Anisakid larvae. FAO 37 zone (Mediterranean sea) showed a prevailing distribution of Anisakis pegreffii and a minimal presence of A. simplex s.s. in hybrid form with Anisakis pegreffii. FAO 27 zone showed a prevailing distribution of A. simplex s.s. in fish like Brosme (Brosme brosme) and infestation prevalence of Pseudoterranova krabbei and P. decipiens s.s. in Gadus morhua. Obtained results validate the hypothesis that molecular biology methods for identifying Anisakidae larvae are effective traceability markers of fish products. © 2015, Ashgate Publishing Ltd. All rights reserved. Source

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