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Mazzette R.,University of Sassari | Fois F.,University of Sassari | Consolati S.G.,University of Sassari | Salza S.,Institute for Experimental Veterinary Medicine of Sardinia | And 6 more authors.
Italian Journal of Food Safety | Year: 2015

Healthy pigs carrying pathogenic to human Yersinia enterocolitica strains are the main source of entry into slaughterhouse, where cross-contamination of carcasses can happen. The aim of this work was to determine Y. enterocolitica prevalence in slaughtered pigs, investigating the presence of carriers in relation to carcass contamination. A total of 132 pig samples (tonsils, mesenteric lymph nodes, colon content, carcass surface) were collected from 4 Sardinian slaughterhouses. All the samples were examined by the ISO 10273:2003 method, and the prevalence was also determined by direct plating on CIN Agar. Moreover, to detect the ail positive Y. enterocolitica strains in enrichment broths and isolates a real-time polymerase chain reaction (PCR) was applied. Y. enterocolitica prevalence was 19% with direct plating and 12% with enrichment methods. Carcass surfaces and tonsils prevalence was 5.30% by direct plating, and 5.3% and 2.2%, respectively, by enrichment method. Tonsil samples showed an average contamination level of 3.2×103 CFU/g, while the mean value on carcass was 8.7×102 CFU/g. An overall prevalence of 9.8% of ail positive Y. enterocolitica broths was detected by RT-PCR, that found a higher prevalence in tonsils (7.5%) with respect to cultural methods, confirming the greater sensitivity of this technique when applied for tonsils and faeces samples. The results show a relatively low pathogenic Y. enterocolitica prevalence in pigs slaughtered in Sardinia. Good hygiene measures should be applied at slaughterhouse in order to prevent the entry of carriers and control carcass contamination. © R. Mazzette et al., 2015.


PubMed | Local Health Unit N1, University of Sassari, Local Health Unit N6 and Institute for Experimental Veterinary Medicine of Sardinia
Type: Journal Article | Journal: Italian journal of food safety | Year: 2016

Healthy pigs carrying pathogenic to human


Tilocca M.G.,Institute for Experimental Veterinary Medicine of Sardinia | Caneglias E.,Institute for Experimental Veterinary Medicine of Sardinia | Vodret B.,Institute for Experimental Veterinary Medicine of Sardinia | Mancuso M.R.,Institute for Experimental Veterinary Medicine of Sardinia | And 3 more authors.
Italian Journal of Food Safety | Year: 2015

The European legislation set the new hygiene standards in food chain with the purpose to ensure high levels of public health protection in relation to food production. In order to guarantee excellent hygiene standards in food chain, particular attention must be paid to the presence of foreign matter, like light solid impurities of mineral, vegetable or animal origin. The light filth test is a suitable method used to detect and count light solid impurities applicable to different foodstuffs. We report the results of the analysis of 93 foodstuffs official samples investigated for the presence of foreign matter at the Institute for Experimental Veterinary Medicine of Sardinia, from 2012 to 2013. Insect fragments were found in a sample of semolina and in a sample of canned tomato; plastic fragments were found in a sample of grated bread. © M.G. Tilocca et al., 2015 Licensee PAGEPress, Italy.


Schiavo M.R.,Institute for Experimental Veterinary Medicine of Sicily | Manno C.,Institute for Experimental Veterinary Medicine of Sicily | Zimmardi A.,Institute for Experimental Veterinary Medicine of Sicily | Vodret B.,Institute for Experimental Veterinary Medicine of Sardinia | And 3 more authors.
Italian Journal of Food Safety | Year: 2015

The presence of foreign bodies in mushrooms affects their marketability and may result in health risks to consumers. The inspection of fresh or dried mushrooms today is very important in view of the increased consumption of this kind of food. Ten samples of dried mushrooms collected in supermarkets were examined for evidence of entomological contamination by macro and microscopic analytical methods, the so-called filth-test. A total of 49 46 determinations, comprising 15 g of the vegetable matrix, were made. The microscopic filth test consistently detected an irregular distribution of physical contaminants following repeated determinations of the same sample. Visual examination, on the other hand, was not sufficient to ensure a product free of contaminants © 2015, Page Press Publications. All rights reserved.


PubMed | University of Sassari, Institute for Experimental Veterinary Medicine of Piedmont and Institute for Experimental Veterinary Medicine of Sardinia
Type: Journal Article | Journal: Italian journal of food safety | Year: 2017

Sardinia (Italy, north-western Mediterranean) is a commercially important producer of edible bivalve molluscs. Since the early 2000s, it was subjected to recurring cases of mussel farm closures due to toxic algal poison. Here, we present the studies on toxin concentrations and the associated potentially toxic phytoplankton distribution and abundances carried out by a regular monitoring programme in Sardinian shellfish areas, from January to May 2015. Diarrheic shellfish poisoning (DSP) toxins were detected in several bivalve molluscs samples, while paralytic shellfish poisoning (PSP) and paralytic shellfish poisoning toxins were present just once, without exceeding the legal limits. Potentially toxic algal species have been constantly present.


PubMed | Institute for Experimental Veterinary Medicine of Sicily and Institute for Experimental Veterinary Medicine of Sardinia
Type: Journal Article | Journal: Italian journal of food safety | Year: 2016

The European legislation set the new hygiene standards in food chain with the purpose to ensure high levels of public health protection in relation to food production. In order to guarantee excellent hygiene standards in food chain, particular attention must be paid to the presence of foreign matter, like light solid impurities of mineral, vegetable or animal origin. The light filth test is a suitable method used to detect and count light solid impurities applicable to different foodstuffs. We report the results of the analysis of 93 foodstuffs official samples investigated for the presence of foreign matter at the Institute for Experimental Veterinary Medicine of Sardinia, from 2012 to 2013. Insect fragments were found in a sample of semolina and in a sample of canned tomato; plastic fragments were found in a sample of grated bread.


PubMed | Institute for Experimental Veterinary Medicine of Umbria and Marche, Institute for Experimental Veterinary Medicine of Sicily and Institute for Experimental Veterinary Medicine of Sardinia
Type: Journal Article | Journal: Italian journal of food safety | Year: 2016

The presence of foreign bodies in mushrooms affects their marketability and may result in health risks to consumers. The inspection of fresh or dried mushrooms today is very important in view of the increased consumption of this kind of food. Ten samples of dried mushrooms collected in supermarkets were examined for evidence of entomological contamination by macro and microscopic analytical methods, the so-called


Terrosu G.,Institute for Experimental Veterinary Medicine of Sardinia | Fadda A.,Institute for Experimental Veterinary Medicine of Sardinia | Fadda A.,Local Health Unit n | Frongia G.,Local Health Unit n | And 2 more authors.
Italian Journal of Food Safety | Year: 2015

Listeria (L.) monocytogenes is frequently isolated from food production environment and often persists in dairy plants despite vigorous sanitation regimes. In recent years several alert notifications were sent to Rapid Alert System for Food Products system as a consequence of Listeria monocytogenes contamination of ricotta cheese. After the alert of 2012, competent authority (Local Health Unit of Sassari Province) organised an environmental monitoring plan with the partnership of the Institute for Experimental Veterinary Medicine of Sardinia to verify analysis of dairy plants own-check according to Regulation (EC) N° 2073/05 and further modifications. In 2014 n. 665 processing areas samples of n. 50 dairy plants of Sassari Province were examined. UNI EN ISO 11290-1:2005 for detection of L. monocytogenes was used. Non-compliance in n. 5 diary plants are observed (n. 8 positive samsamples). Post-non-compliance environmental sanitisation was efficient and own-check plans included appropriate corrective actions. © G. Terrosu et al., 2015.

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