Institute for Experimental Veterinary Medicine of Lombardy and Emilia Romagna

Brescia, Italy

Institute for Experimental Veterinary Medicine of Lombardy and Emilia Romagna

Brescia, Italy

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PubMed | Institute for Experimental Veterinary Medicine of Lombardy and Emilia Romagna, University of Bologna and Parmigiano Reggiano Cheese Consortium
Type: Journal Article | Journal: Italian journal of food safety | Year: 2016

This study investigated aflatoxin B1 (AFB1) contamination in dairy cow feed and the risk management of AFB1 content in concentrates undertaken by feed industries in the


Bonilauri P.,Institute for Experimental Veterinary Medicine of Lombardy and Emilia Romagna | Bardasi L.,Institute for Experimental Veterinary Medicine of Lombardy and Emilia Romagna | Leonelli R.,Institute for Experimental Veterinary Medicine of Lombardy and Emilia Romagna | Ramini M.,Institute for Experimental Veterinary Medicine of Lombardy and Emilia Romagna | And 3 more authors.
Italian Journal of Food Safety | Year: 2016

Foodstuffs should not contain microorganisms or their toxins or metabolites in quantities suggesting an unacceptable risk for human health. The detection of food hazards in foods is performed by several tests that produce results dependent on the analytical method used: an analytical reference method, defined as standard, is associated with each microbiological criterion laid down in Regulation 2073/2005/EC, but, analytical methods other than the reference ones, in particular more rapid methods, could be used. Combined screening methods performed by real time-polymerase chain reaction (RT-PCR) are currently validated as alternative methods according to the ISO 16140:2003 and certified by the Association Française de Normalisation. However, the positive results obtained with these alternative methods, the investigated molecular relations that resulted positive have to be confirmed with cultural methods using the same enrichment media in which the molecular screening was performed. Since it is necessary to assess if these testing schemes provide equivalent guarantees of food safety, the aim of this retrospective study is to analyse the data collected, from 2012 to 2014 by Emilia Romagna Region in the field of Piano Regionale Alimenti (Food Regional Plan) during official controls monitoring food samples of animal and other than animal origin. Records performed by combined methods of molecular screening of Salmonella spp., Listeria monocytogenes and thermophilic Campylobacter and cultural confirmation results were gathered together and the results were compared in order to assess the sensitivity of the methods. A total of 10,604 food samples were considered in this study: the comparison of the data revealed that the RT-PCR method detected Salmonella, L. monocytomonocytogenes, and thermophilic Campylobacter in 2.18, 3.85 and 3.73% of the samples, respectively, whereas by using cultural method these pathogens were isolated in 0.43, 1.57 and 1.57% of samples, respectively. In spite of the use of the same enrichment broth, the RT-PCR method disclosed a percentage of positive samples that was negative to cultural examination ranging between 20 and 43%, with a PCR/culture ratio between 2.37 to 5.00. In conclusion, the results of this study pose a doubt about the sensitivity of the official cultural methods regarding the isolation of the three investigated foodborne pathogens. Moreover this study may be a useful tool for veterinary authorities to assess appropriate sampling plans to control the risk relating to the consumption of contaminated foods. © P. Bonilauri et al., 2016.


Merialdi G.,Institute for Experimental Veterinary Medicine of Lombardy and Emilia Romagna | Ramini M.,Institute for Experimental Veterinary Medicine of Lombardy and Emilia Romagna | Ravanetti E.,Ham factory San Michele SRL | Gherri G.,Ham factory San Michele SRL | Bonilauri P.,Institute for Experimental Veterinary Medicine of Lombardy and Emilia Romagna
Italian Journal of Food Safety | Year: 2015

The present work aims to present the results of the application of a treatment with high hydrostatic pressure (HHP) on Italian fermented and dry-cured pork products. The products used in this study were portioned cured ham, portioned bacon and salami, vacuum-packaged and produced by a single processing company. Two studies were conducted on a single batch of the three products by means of an artificial contamination with Listeria innocua as a surrogate of L. monocytogenes. In the first trial a superficial contamination was obtained by immersion for 3 min in the culture broth with a concentration of approximately 9 log cfu/mL. At the end of the inoculum step, the pieces were dred at room temperature and vacuum packaged. In the second trial 50 kg of minced pork meat were contaminated before production of salami. In both cases the inoculum contained 5 strains of L. innocua. Subsequently, in both trials, 10 samples were randomly divided into two groups of 5 pieces each: i) TH group, samples treated with HHP; ii) group C, control samples, not subjected to any treatment. All samples were stored at refrigeration temperature at the end of HHP treatments (if applied), and analyzed for the determination of the surface (1st trial) and deep (2nd trial) quantitative contamination of L. innocua. pH and aW were also determined on 3 pieces of each products belonging to group C. The difference between the medians of the log cfu/cm2 or g established between controls and treated were compared using the non-para- metric test (Kruskal-Wallis test) with P<0.01. In all products and in both trials the level of contamination detected in treatment groups was always significantly lower than in controls (P<0.01). In particular, in vacuum-packaged ham, bacon and salami viability logarithmic viability reductions equal to -2.29, -2.54 and -2.51 were observed, respectively. This study aimed to evaluate a not-thermal treatment on Italian cured or fermented pork products. The results of this study need to be confirmed in different products and in a greater number of lots, but they appear promising, also because of the considerable literature available for different categories of products (cheese, vegetables and fruit) © G. Merialdi et al., 2015


Pavoni E.,Institute for Experimental Veterinary Medicine of Lombardy and Emilia Romagna | Barbieri I.,Institute for Experimental Veterinary Medicine of Lombardy and Emilia Romagna | Bertasi B.,Institute for Experimental Veterinary Medicine of Lombardy and Emilia Romagna | Lombardi G.,Institute for Experimental Veterinary Medicine of Lombardy and Emilia Romagna | And 2 more authors.
Italian Journal of Food Safety | Year: 2015

Hepatitis E virus (HEV) is an important public health concern in many developing countries and it occurs in sporadic forms in industrialized areas. With the discovery of swine HEV in pigs, which is genetically closely related to human HEV, hepatitis E is considered to be a zoonotic disease. To investigate the circulation of HEV within a distinct area of Lombardy region (Northern Italy), 17 pig farms were subjected to monitoring study by collection of fresh stool samples each represented by ground-pooled specimens. In particular, three distinct types of breeding farms were focused, represented by farrow to weaning, farrow to finish and fattening farms, respectively. Epidemiological data confirm that in Europe the seroprevalence in pigs, more than 9 month of age, ranges from 51.4 to 75%, while in 3-9 months fatteners is about 38%. In France and Italy, the positivity among farms is respectively 30 and 97.4% and the seroprevalence in Italy is 50.2%. Since HEV viremia was typically observed in the early period of life in swine, faeces were collected in boxes containing weaning pigs. For the study, 183 stool samples were collected and amplifications were performed with universal primers specific for the ORF2 region of genome. Twenty-eight samples resulted positive to HEV RNA and genotyping demonstrated that they were closely related to HEV strains belonging to genotype 3 and circulating in Europe. Comparison with reference strains from GenBank excluded their similarity to genotype 1, 2 or 4 confirming that genotype 3 strains are circulating in Europe. Since it was demonstrated that swine act as a reservoir for HEV, and since many strains into HEV genotype 3 share a strong molecular similarity to human HEV, it was important to detect the presence of HEV in a restricted area with a very high density of pigs. © 2015, Page Press Publications. All rights reserved.


PubMed | University of Bologna and Institute for Experimental Veterinary Medicine of Lombardy and Emilia Romagna
Type: Journal Article | Journal: Italian journal of food safety | Year: 2016

Foodstuffs should not contain microorganisms or their toxins or metabolites in quantities suggesting an unacceptable risk for human health. The detection of food hazards in foods is performed by several tests that produce results dependent on the analytical method used: an analytical reference method, defined as standard, is associated with each microbiological criterion laid down in Regulation 2073/2005/EC, but, analytical methods other than the reference ones, in particular more rapid methods, could be used. Combined screening methods performed by real time-polymerase chain reaction (RT-PCR) are currently validated as alternative methods according to the ISO 16140:2003 and certified by the


PubMed | Parma Local Health Unit, Institute for Experimental Veterinary Medicine of Lombardy and Emilia Romagna and Experimental Unit for Food Preserves Industry
Type: Journal Article | Journal: Italian journal of food safety | Year: 2016

The objective of this study was to investigate


PubMed | University of Parma and Institute for Experimental Veterinary Medicine of Lombardy and Emilia Romagna
Type: Journal Article | Journal: Italian journal of food safety | Year: 2016

Ninety pig carcasses and twenty one food contact surfaces (FCSs) were tested for


PubMed | Institute for Experimental Veterinary Medicine of Lombardy and Emilia Romagna and Ham factory San Michele SRL
Type: Journal Article | Journal: Italian journal of food safety | Year: 2016

The present work aims to present the results of the application of a treatment with high hydrostatic pressure (HHP) on Italian fermented and dry-cured pork products. The products used in this study were portioned cured ham, portioned bacon and salami, vacuumpackaged and produced by a single processing company. Two studies were conducted on a single batch of the three products by means of an artificial contamination with


PubMed | Institute for Experimental Veterinary Medicine of Lombardy and Emilia Romagna
Type: Journal Article | Journal: Italian journal of food safety | Year: 2016

The FLOQSwab is a specimen collection device worldwide recognised for its superior performance in the clinical diagnostics. The aim of this work was to evaluate FLOQSwab for the recovery of microbiological samples from surfaces compared to the traditional swab (rayon tipped swab) as per ISO 18593:2004 standard. The FLOQSwab, thanks to its innovative manufacturing technology, allows improving the efficiency of recovery and release of analyte. The study has been divided into two experiments. In the first experiment the two swabs were evaluated for their capacity to recover and release the analyte (three different bacterial loads of


PubMed | University of Bologna and Institute for Experimental Veterinary Medicine of Lombardy and Emilia Romagna
Type: Journal Article | Journal: Italian journal of food safety | Year: 2016

In temperate climates, a seasonal trend was observed in the incidence of human campylobacteriosis cases, with peaks reported in spring and autumn in some countries, or in summer in others; a similar trend was observed in

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