Institute for Diagnosis and Animal Health

Bucharest, Romania

Institute for Diagnosis and Animal Health

Bucharest, Romania
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Crenguta Nicolae A.,Carol Davila University of Medicine and Pharmacy | Mitrea N.,Carol Davila University of Medicine and Pharmacy | Manuela Dragoi C.,Carol Davila University of Medicine and Pharmacy | Zinaida Constantinescu M.,Carol Davila University of Medicine and Pharmacy | And 3 more authors.
Farmacia | Year: 2013

The imbalance between the production and accumulation of reactive oxygen species and the body's ability to manage them by exogenous and endogenous antioxidant defense systems leads to oxidative stress. An important source to generate reactive oxygen species (ROS), as superoxide anions (O2 ·-) and hydrogen peroxide (H2O2) is the mitochondrial and microsomal system. The present study assessed the variation of oxidative status in mice, after the intraperitoneal administration of some CNS active drugs. Serum, hepatic and brain samples were used throughout the study. A chemiluminescent method was applied in order to evaluate the oxidative status. Despite the fact that the brain is considered highly sensitive to oxidative damage, explained by the large amount of readily oxidizable fatty acids and poor antioxidant defenses, in our study, for cerebral and hepatic tissues we proved that the administration of tested drugs led to a decrease of the oxidative status, while in the case of serum it was developed an increase of ROS concentrations. The oxidative stress attack limitation using therapeutic agents with additional antioxidant effect, could be a therapeutic option leading to reduced doses and adverse effects of some CNS active drugs.


Nicolae A.C.,Carol Davila University of Medicine and Pharmacy | Mitrea N.,Carol Davila University of Medicine and Pharmacy | Arsene A.L.,Carol Davila University of Medicine and Pharmacy | Constantinescu M.Z.,Carol Davila University of Medicine and Pharmacy | And 2 more authors.
Farmacia | Year: 2013

P-glycoprotein (Pgp), also called MDR1, the product of the multidrug resistance (MDR) gene, is an ATP-dependent efflux transporter that affects the absorption, distribution, and excretion of a great number of clinically important drugs. Modulation of P-glycoprotein (Pgp) through inhibition or induction can lead to interactions at the level of the central nervous system (CNS) or intestinal, renal, or biliary efflux, having consequences upon treatment optimization. In order to reveal the Pgp effect of some CNS important drugs, we investigated five structurally diverse active substances: valproic acid (V), fluoxetine hydrochloride (F), risperidone (R), thioridazine hydrochloride (T) and lithium carbonate (L) for their ability to inhibit Pgp, compared to quinidine (Q), a classic Pgp inhibitor. Pgp inhibition was studied in temporal dynamics (different cell-drug contact times), by a fluorimetric assay using calcein-acetoxymethylester as fluorophore, on the murine neuroblastoma cell line N2a. Modulation of P-glycoprotein was accomplished using a physiological concentration of glutathione (GSH), on the cell line treated with the studied drugs.


Barbuceanu S.-F.,Carol Davila University of Medicine and Pharmacy | Bancescu G.,Carol Davila University of Medicine and Pharmacy | Saramet G.,Carol Davila University of Medicine and Pharmacy | Radulescu F.S.,Carol Davila University of Medicine and Pharmacy | And 3 more authors.
Farmacia | Year: 2016

In this paper we present the antimicrobial activity screening of a series of eighteen compounds from hydrazinecarbothioamides (1-3), 1, 2, 4-triazole-3-thiones (4-6), S-alkylated 1, 2, 4-triazoles (7-12), 1, 3, 4-thiadiazoles (13-15) and 1, 3, 4-oxadiazoles (16-18) class which contain arylsulfonylphenyl and 2, 4-difluorophenyl moieties in their molecule. The antimicrobial action of these compounds has been investigated against the following reference microbial strains: Escherichia coli ATCC 25922, Enterobacter cloacae ATCC 49141, Acinetobacter baumannii ATCC 19606, Pseudomonas aeruginosa ATCC 27853, Bacillus cereus ATCC 13061, Staphylococcus aureus ATCC 29213, Candida albicans ATCC 90028, Candida glabrata ATCC 64677, Candida krusei ATCC 14243 and Candida parapsilosis ATCC 22019, by the broth microdilution method. Comparing the values of the minimum inhibitory concentrations of the compounds it was noticed that triazoles 4-6 and hydrazinecarbothioamide 2 had in general the best antibacterial activity against some tested bacteria (MIC of 32 μg/mL) and oxadiazole 17 showed the best antifungal activity against C. parapsilosis (MIC of 32 μg/mL). © 2016, Romanian Society for Pharmaceutical Sciences. All rights reserved.


Draghici C.,Romanian Academy of Sciences | Barbuceanu F.,Institute for Diagnosis and Animal Health
European Journal of Medicinal Chemistry | Year: 2012

Some new 5-(4-(4-X-phenylsulfonyl)phenyl)-4-(R)-2H-1,2,4-triazol-3(4H)- thiones 4a,b; 5a,b and 5-(4-(4-X-phenylsulfonyl)phenyl)-N-(R)-1,3,4-thiadiazol- 2-amines 6a,b; 7a,b were obtained by cyclization of new N 1-[4-(4-X- phenylsulfonyl)benzoyl]-N 4-(R)-thiosemicarbazides 2a,b; 3a,b (X = H, Br). The 1,2,4-triazoles were synthesized by intramolecular cyclization of acylthiosemicarbazides, in basic media. On the other hand, 1,3,4-thiadiazoles were obtained from same acylthiosemicarbazides, in acidic media. These new intermediates from thiosemicarbazide class were afforded by the reaction of 4-(4-X-phenylsulfonyl)benzoic acids hydrazides (X = H, Br) 1a,b with 4-trifluoromethoxyphenyl or 3,4,5-trimethoxyphenyl isothiocyanate. The newly synthesized compounds were characterized by IR, 1H NMR, 13C NMR, MS and elemental analysis. All the new compounds were screened for their antimicrobial activity against some bacteria (Staphylococcus aureus ATCC 25923, Bacillus cereus ATCC 13061, Escherichia coli ATCC 25922, Enterobacter cloacae ATCC 49141, Acinetobacter baumannii ATCC 19606 and Pseudomonas aeruginosa ATCC 27853) and yeasts (Candida albicans ATCC 90028 and Candida parapsilosis ATCC 22019). © 2012 Elsevier Masson SAS. All rights reserved.


Chitimia-Dobler L.,Institute for Diagnosis and Animal Health | Chitimia-Dobler L.,University of Federal Defense Munich
Veterinary Parasitology | Year: 2015

Dermacentor reticulatus (Fabricius, 1794), also known as the marsh tick or ornate dog tick is the second most significant vector (next to Ixodes ricinus) of protozoan, rickettsial and viral pathogens in Europe. Until now, only limited information on the distribution of D. reticulatus in Romania is available. A study was conducted on the distribution of D. reticulatus in Romania during 2012-2014. In this study, D. reticulatus was detected in 17 counties, in 14 of which the species was recorded for the first time. Tick activity was evident throughout the year, except during July and August. Additionally, D. reticulatus was recorded for the first time in Romania from wild boar, foxes and humans. These data suggest that this tick species has a broader geographic range and may have more veterinary and medical importance than previously known. © 2015 Elsevier B.V.


PubMed | Spiru Haret University, Romanian National Institute for Lasers, Plasma and Radiation Physics and Institute for Diagnosis and Animal Health
Type: Journal Article | Journal: Medical engineering & physics | Year: 2016

Reproducible animal models of osteomyelitis close to the clinical scenario are difficult to obtain as the animals either die shortly after inoculation of bacteria or the bone cures itself of infection. Additional materials used as foreign bodies offer increased chances for localized infection due to bacterial attachment and are closer to clinical pathology. Through in vivo experimentation we investigated here the influence of surface area of a series of foreign bodies on the final outcome of the animal model, in terms of reproducibility, survival rate and time necessary for onset of chronic disease. Stainless steel Kirschner wire segments, stainless steel balls and cotton meshes were employed for this purpose. The clinical, microbiological, radiological and histological results obtained were compared with the simple case where no foreign body was used. The follow-up period was 57days. The cotton meshes, which had the highest surface area, were observed to provide the best outcome, with the lowest disease onset time interval (of 1week earlier than the others), the highest survival (of 90%) and disease reproduction rate (90%). The only clinical pattern of the mesh group rabbits was short lived inflammation while the other rabbits presented also some other clinical signs such as rhinorrheas, abscesses, rush and/or dyspnea. Moreover, this model is the most suitable for further treatment studies, as the cotton meshes could be easily removed after disease onset, without any intervention on the bone. This is important, as the treatment would address the bacteria present within the bone parts (marrow, cortex, periosteum etc.) not those forming the biofilm.


Andersson M.,Lund University | Turcitu M.A.,Institute for Diagnosis and Animal Health | Stefanache M.,PAUMI VET Private Veterinary Clinics | Tamba P.,Institute for Diagnosis and Animal Health | And 2 more authors.
Ticks and Tick-borne Diseases | Year: 2013

Anaplasma platys was first identified and described in North America as a Rickettsia-like, platelet-specific organism in dogs with infectious canine cyclic thrombocytopenia. In Europe, A. platys has so far mainly been described for some Mediterranean countries. Here, we describe a case of A. platys infection in a dog from Romania, confirmed by PCR. Additionally, the dog had a co-infection with Hepatozoon canis. To the best of our knowledge, this is the first case of A. platys infection in Romania and the first case of a co-infection with A. platys and H. canis altogether. Both pathogens should be considered as possible disease agents in dogs suffering from disease associated with tick bite in south-eastern Europe. © 2013 Elsevier GmbH.


Casulli A.,Instituto Superiore Of Sanita | Interisano M.,Instituto Superiore Of Sanita | Sreter T.,Laboratories for Parasitology | Chitimia L.,Institute for Diagnosis and Animal Health | And 3 more authors.
Infection, Genetics and Evolution | Year: 2012

The genetic diversity of Echinococcus granulosus sensu stricto (s.s.) metacestodes from four European countries was evaluated by the DNA sequence analysis of the cytochrome c oxidase subunit 1 (cox1) mitochondrial gene. Of the 312 organisms investigated, 132 were from Bulgaria, 35 from Hungary, 89 from Italy and 56 from Romania. Considerable intraspecific variation was observed in the mitochondrial cox1 sequences: 24 haplotypes were detected in the Eastern European population and seven in the Italian population. The Eastern European population parsimony network displayed a star-like features consisting of the most common haplotype EG1 (G1 genotype) and the three major haplotypes: EG2, EG3 and EG4. The EG1 was also the major haplotype in the Italian population network, though with a higher prevalence (73%) compared to the Eastern European network. The percentage of the population constituted by the G1 genotype was used as an indirect index to evaluate the genetic diversity within E. granulosus s.s. populations of Eurasia. A clinal correlation between the percentage of the G1 genotype and the geographical regions of Eurasia was observed: the G1 genotype is highly represented in the Mediterranean Basin; it decreases in Eastern Europe and South-West Asia and increases in China. This clinal correlation could reflect the spreading of livestock domestication from Southern-Western Asia during the Neolithic period, beginning around 12,000. BC. © 2012 Elsevier B.V.


Mitrea I.L.,University of Agronomical Sciences and Veterinary Medicine | Enachescu V.,University of Agronomical Sciences and Veterinary Medicine | Radulescu R.,University of Agronomical Sciences and Veterinary Medicine | Radulescu R.,Institute for Diagnosis and Animal Health | Ionita M.,University of Agronomical Sciences and Veterinary Medicine
Journal of Parasitology | Year: 2012

Neospora caninum, a coccidian parasite closely related to Toxoplasma gondii, is one of the major causes of abortion in cattle worldwide. Conventional serological techniques, such as the indirect fluorescent antibody test and enzyme-linked immunosorbent assay (ELISA), are routinely used in adult animals and aborted fetuses for the detection of antiN. caninum antibodies. In Romania, infection with N. caninum in cattle has been reported recently, but only in limited areas from the north and central parts of the country. Therefore, the aim of this study was to obtain additional seroepidemiological data on infection with N. caninum on dairy farms from the south of Romania. A total of 258 blood samples was analyzed from 230 dairy cows and 28 calves from 9 dairy farms in southern Romania; the presence of specific IgG antibodies against N. caninum was determined using an indirect ELISA test. The average seroprevalence was 40.3%, but the within-herd prevalence ranged between 11.5 and 80.0%; the seroprevalence in dairy cows was 41.7%, while in calves it was 28.6%. Of the positive samples, 74.0% (77/104) had a high positive reaction (S/P ratio more than 1.0), while 26.0% (27/104) had a low positive reaction (S/P ratio between 0.5 and 1.0). This study indicates that N. caninum infection is widespread in the south of Romania, which could explain the causes of abortions registered in some herds in the studied area. However, a serological screening across the country is planned in order to assess the actual national prevalence of N. caninum infection, followed by implementation of a prevention and control program. © American Society of Parasitologists 2012.


Andersson M.,Lund University | Zaghdoudi-Allan N.,Lund University | Tamba P.,Institute for Diagnosis and Animal Health | Stefanache M.,Postdoctoral School for Zootechnical Biodiversity and Food Biotechnology | Chitimia L.,Institute for Diagnosis and Animal Health
Ticks and Tick-borne Diseases | Year: 2014

Despite the vast importance of ticks as disease vectors, the infectious agents transmitted by ticks are still incompletely known in many areas. Here, we report for the first time the detection of the bacterium '. Candidatus Neoehrlichia mikurensis' in Romania, in an Ixodes ricinus tick obtained from a human. Furthermore, the tick also had a co-infection with Borrelia afzelii. '. Candidatus Neoehrlichia mikurensis' is one of the most recent discoveries of a tick-borne agent, and has been found in human patients in several European countries as well as in China. © 2014 Elsevier GmbH.

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