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Vojvodic S.,Institute for Blood Transfusion of Vojvodina
Genetika | Year: 2011

The polymorphism at the HLA DRB1 and DQB1 loci in the population of Vojvodina was studied by PCR-SSP method. A total of 13 DRB1 and 5 DQB1 specificities displaying population-specific frequency distribution pattern were described. The most frequent HLA Class II alleles in Vojvodina population were: HLA-DRB1*11 (af = 0.30), -DRB1*04 (af = 0.28), -DRB1*07(af = 0.21), -DRB1*13 and -DRB1*16 (af = 0.18), -DQB1*03 (af = 0.64), -DQB1*05 (af = 0.39) and -DQB1*02 (af = 0.35). The haplotypes with high frequencies (> or = 0.02) included HLA DRB1*11 DQB1*03 (0.0825), DRB1*04DQB1*03 (0.0725), DRB1*07DQB1*02 (0.0475). The allele DRB1*07 showed the strongest association with DQB1*02 (delta = 0.0261, chi2 = 4.437) and DRB1*13 allele with DQB1*06 (delta = 0.0222, chi2 = 4.247). The allelic frequencies and populations distance dendrogram revealed the closest relationship of Vojvodina population with Hungarians, Croat, and Greeks which can be the result of turbulent migration within this region and admixture with neighbour populations during the history. Source


Vojvodic S.,Institute for Blood Transfusion of Vojvodina | Ademovic-Sazdanic D.,Institute for Blood Transfusion of Vojvodina | Busarcevic I.,University of Novi Sad
Genetika | Year: 2013

Major histocompatibility complex (MHC) genes are involved in various mechanisms of pathogenesis and immunoediting of hematological diseases. This study aimed to investigate the association between HLA -A, -B and -DRB1 alleles with hematological diseases. In this study, we performed DNA-based HLA typing by polymerase chain reaction analysis with sequence-specific primers (PCR-SSP) to distinguish HLA-A, -B, and -DRB1 alleles. Eighty-two patients with hematological diseases (29 with acute lymphoblastic leukemia (ALL), 19 with acute nonlymphoblastic leukemia (ANLL), 5 with chronic myelogenous leukemia (CML), 2 with chronic lymphocytic leukemia (CLL), 9 with myelodysplastic syndrome (MDS), 9 with lymphomas (M.Hodgkin (HL) and non-Hodgkin (NHL)), 7 with aplastic anemia (AA) and 2 with multiple myeloma (MM)), were included in the study and compared with 111 healthy blood donors, residents from Vojvodina, evaluating the strength of the observed associations by measuring the aetiologic and preventive fractions. Among the alleles significantly associated with hematological diseases, HLA-A*24 showed an aetiologic fraction higher than those of HLA-A*26 and A*25 (RR=1.027, EF=1.233, RR=1.047, EF=1.141 and RR=1.213, EF=0.910).Negative association with significant preventive fraction was observed with HLA-B*18 and HLA-DRB1*11 alleles, with RR=0.400, PF=0.179 and RR=0.587, PF=0.176. Our results suggest that HLA-A*24, A*26 and A*25 as associated more frequently than other specificities with a hypothetical disease predisposing genes, may play a role in the pathogenesis of hematological diseases. Source


Vojvodic S.I.,Institute for Blood Transfusion of Vojvodina | Ademovic-Sazdanic D.S.,Institute for Blood Transfusion of Vojvodina | Popovic S.L.,Institute for Internal Medicine | Konstantinidis N.V.,Oncology Institute of Vojvodina
Annals of Human Biology | Year: 2015

Background: Killer cell immunoglobulin-like receptors (KIRs) are glycoproteins regulating the response of natural killer (NK) cells and a few sub-sets of T-cells. The KIR gene frequencies and genotype content vary considerably among different ethnic groups.Aim: The aim of this study was to analyse KIR gene polymorphism in the population of Vojvodina and to compare it with selected worldwide populations.Subjects and methods: The studied sample consists of 134 healthy unrelated individuals, residents of different geographical regions of Vojvodina. DNA samples isolated from peripheral blood leukocytes by the silica-based extraction method were used in reverse PCR-SSO and PCR-SSP technique to detect the presence and absence of KIR genes.Results: All 16 KIR genes, a total of 37 different KIR genotypes, were observed in the Vojvodina population with the presence of framework and pseudogenes in all individuals. The neighbour-joining phylogenetic tree shows that the Vojvodina population is in the same cluster with Croatians, Turkish, Russians, Czechs, Irish, Italians, French, Macedonians and Polish. The Vojvodina population shows polymorphism of the KIR gene family present in other European and European-derived populations studied previously.Conclusion: The present study may serve as a reference for comparisons in further anthropological and disease association studies and also provide more informative data valuable for donor search strategy in haematopoietic stem cell transplantation. © 2014 © 2014 Informa UK Ltd. Source


Vasic N.G.,General Hospital Dorde Joanovic | Vojvodic S.I.,Institute for Blood Transfusion of Vojvodina | Ademovic-Sazdanic D.S.,Institute for Blood Transfusion of Vojvodina
Central European Journal of Medicine | Year: 2013

Objective: The preformed antibodies detection in potential kidney recipients is necessary in pretransplant evaluation. The aim of this study was to determine the correlation between the application of blood transfusions and sensitization level in potential kidney transplants. Methods: The study included 268 potential kidney transplants from the region of Vojvodina. The presence of preformed antibodies was tested by microlymphocitotoxicity test. Pearson's correlation coefficient was applied to determine the influence of three variables on sensitization level: number and volume of transfused blood units and time elapsed from last transfused unit. Results: Of 268 eligible patients, 206 patients had a history of blood transfusion. Results of the study showed that the application of great number and volume of transfused units increased the sensitization level (correlation coefficients were r = +0,283 and r = +0,235, respectively) with statistical significance as well as the negative correlation between the time elapsed from the last transfusion and the degree of sensitization (r = -0,082) with no statistical significance. Conclusion: Our study revealed that there is a stronger positive correlation between the number of received blood units than the volume of received blood products and the level of preformed antibodies. © 2013 Versita Warsaw and Springer-Verlag Berlin Heidelberg. Source


Vojvodic S.,Institute for Blood Transfusion of Vojvodina | Ademovic-Sazdanic D.,Institute for Blood Transfusion of Vojvodina
Central European Journal of Medicine | Year: 2013

Background: The reactivity between donor's Human Leukocyte Antigen (HLA) and recipient's anti-HLA antibody in pretransplantation assessment is one of the critical factors to determining successful outcome of renal transplantation. Objective: The aim of present study was to compare different techniques of HLA antibody detection in patients waiting for a kidney transplant. Methods: Two techniques of HLA antibody screening were compared: the complement-dependent citotoxicity (CDC) test and enzyme-linked immunosorbent assay (ELISA). The study included 606 sera samples of 236 patients waiting for a first kidney transplantation. Results: Of 606 tested sera, 469 (77.39%) were negative by both methods. Of the 137 (22.6%) positive sera, 73 (12.04%) were positive only by ELISA method, 48 (7.92%) by both CDC and ELISA methods and 16 (2.64%) only by CDC method. There was a significant (p<0.05) correlation between optical densities obtained by ELISA and the PRA determined by cytotoxicity testing. Conclusion: Fast and precise characterisation of antibodies in patients before transplantation can be performed by both methods, CDC and ELISA, as complementary techniques. ELISA method is more sensitive and effective than CDC, enabling significant reduction of the CDC workload of the laboratory, but can be used only as adjunct to serum screening by citotoxic testing. © 2013 Versita Warsaw and Springer-Verlag Berlin Heidelberg. Source

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