Institute For Bakterielle Infektionen Und Zoonosen

Jena, Germany

Institute For Bakterielle Infektionen Und Zoonosen

Jena, Germany
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Alter T.,Free University of Berlin | Bereswill S.,Charité - Medical University of Berlin | Glunder G.,Klinik fur Geflugel | Haag L.-M.,Charité - Medical University of Berlin | And 7 more authors.
Bundesgesundheitsblatt - Gesundheitsforschung - Gesundheitsschutz | Year: 2011

Over the last few years, infections with Campylobacter have significantly increased in Europe and Germany and these bacteria have even surpassed Salmonella as the most prevalent bacteria, causing gastroenteritis. Especially contamination during the handling and consumption of meat products seems to be the most important risk factor which plays a prominent role for transmission to man. In addition, contact with pets and other animals, drinking raw or improperly pasteurized milk, and the tenacity of Campylobacter in different environments, especially water, have also to be considered for an adequate risk assessment. Besides gastroenteritis, arthralgia, and Guillain-Barré syndrome are important clinical complications of Campylobacter infections in man. At the same time, it is mostly unclear why the course of infection in man and in reservoir animals differs significantly, especially as only a few classical bacterial virulence factors have been identified so far. For these reasons, the development of efficient prevention strategies is of utmost importance in order to control campylobacteriosis. © 2011 Springer Medizin Verlag.


Jandowsky A.,Tierpark Arche Warder | Bodenthin A.,Landeslabor Schleswig Holstein | Seyboldt C.,Institute For Bakterielle Infektionen Und Zoonosen | Frolich K.,Tierpark Arche Warder
Tierarztliche Praxis Ausgabe G: Grosstiere - Nutztiere | Year: 2013

In an outdoor pig-breeding unit of the Tierpark Arche Warder e. V. (Germany), 16 pigs of different age and sex died in October 2011. Necropsy findings revealed tympany, liver emphysema, subcutaneous oedema, haemopericardium, haemothorax, and intense gas bubble infiltrations in muscles. The stomachs were filled. The initial anaerobic bacteriological investigations gave negative results. In further analyses of tissue samples, the flagellin gene of C. novyi types A and B was detected using PCR. Based on the anatomical-pathological and bacteriological findings as well as PCR testing, a C. novyi infection was assumed to be the cause of the pig mortality. © 2013 Schattauer.


Backsch C.,Universitatsklinikum Jena | Pauly B.,Universitatsklinikum Jena | Pauly B.,Institute For Bakterielle Infektionen Und Zoonosen | Liesenfeld M.,Universitatsklinikum Jena | And 8 more authors.
Cancer Genetics | Year: 2011

Chromosomal aberrations are a hallmark of human papillomavirus (HPV)-induced cervical carcinogenesis. The aim of this project was to identify structural chromosomal aberrations which may be characteristic for intraepithelial neoplasias (CIN) and cervical carcinomas (CxCa). Two independent HPV16 immortalized keratinocyte cell lines (HPKIA, HPKII) were used as a cell culture model system for cervical carcinogenesis. Different passages of HPKIA and HPKII were analyzed by multicolor spectral karyotyping. Several chromosomal translocations were identified in HPK cells and were validated by interphase fluorescence in situ hybridization (I-FISH). Three unbalanced whole chromosome arm translocations, der(10;14), der(7;21), and der(7;12), were cell line specific. The presence and frequency of these translocations were then examined by I-FISH in frozen tissue sections from normal cervical epithelia (n = 6), CIN2/3 (n = 15), and CxCa (n = 15). The der(10;14) and der(7;21) were detected in 80% and 53.3% of CIN2/3, and in 60% and 46.7% of CxCa, respectively. The percentage of nuclei with translocations in individual lesions was significantly higher among CxCa. The der(7;12) could only be detected in 27% of CIN2/3. None of the translocations were detected in normal cervical epithelia. The translocated chromosomes may contribute to the clonal expansion of subpopulations in these cases and may thus be of diagnostic relevance. © 2011 Elsevier Inc.


Hilbert A.,Institute For Epidemiologie | Schmoock G.,Institute For Bakterielle Infektionen Und Zoonosen | Lenzko H.,Institute For Molekulare Pathogenese | Moog U.,Tiergesundheitsdienst | And 9 more authors.
BMC Research Notes | Year: 2012

Background: Current epidemiological data on the situation of Coxiella (C.) burnetii infections in sheep are missing, making risk assessment and the implementation of counteractive measures difficult. Using the German state of Thuringia as a model example, the estimated sero-, and antigen prevalence of C. burnetii (10% and 25%, respectively) was assessed at flock level in 39/252 randomly selected clinically healthy sheep flocks with more than 100 ewes and unknown abortion rate. Results: The CHECKIT Q-fever Test Kit identified 11 (28%) antibody positive herds, whereas real-time PCR revealed the presence of C. burnetii DNA in 2 (5%) of the flocks. Multiple-locus variable number of tandem repeats analysis of 9 isolates obtained from one flock revealed identical profiles. All isolates contained the plasmid QpH1. Conclusions: The results demonstrate that C. burnetii is present in clinically inconspicuous sheep flocks and sporadic flare-ups do occur as the notifications to the German animal disease reporting system show. Although C. burnetii infections are not a primary veterinary concern due to the lack of significant clinical impact on animal health (with the exception of goats), the eminent zoonotic risk for humans should not be underestimated. Therefore, strategies combining the interests of public and veterinary public health should include monitoring of flocks, the identification and culling of shedders as well as the administration of protective vaccines. © 2012Sprague et al; licensee BioMed Central Ltd.


Hanel I.,Institute For Bakterielle Infektionen Und Zoonosen | Tomaso H.,Institute For Bakterielle Infektionen Und Zoonosen | Neubauer H.,Institute For Bakterielle Infektionen Und Zoonosen
Bundesgesundheitsblatt - Gesundheitsforschung - Gesundheitsschutz | Year: 2016

The relevance for public health of the agent Arcobacter is mostly unclear despite of an increasing number of studies. Recent evidence shows that especially Arcobacter (A.) butzleri but also A. cryaerophilus and A. skirrowii may be involved in human enteric diseases. However, little is currently known about pathogenicity or potential virulence factors. Livestock animals, particularly poultry and pigs, might be a significant reservoir of Arcobacter spp. Furthermore, Arcobacter spp. could be isolated from retail raw meat products of these animals as well as from drinking water. There are currently no standardized isolation and detection methods to collect comparable data. Further studies and efforts of both human and veterinary medicine are needed to elucidate prevalence, epidemiology, the pathogenic role and potential virulence factors of Arcobacter spp. These data are the necessary basis for further risk assessment. © 2016, Springer-Verlag Berlin Heidelberg.


Dobler G.,University of Federal Defense Munich | Fingerle V.,Nationales Referenzzentrum fur Borrelien | Hagedorn P.,Robert Koch Institute | Pfeffer M.,University of Leipzig | And 4 more authors.
Bundesgesundheitsblatt - Gesundheitsforschung - Gesundheitsschutz | Year: 2014

Tick-transmitted diseases are of great importance for the general health of the German population. Several viruses, such as tick-borne encephalitis virus (TBEV), Uukuniemi virus, Tribec virus, Eyach virus or bacteria, such as Borrelia, Rickettsiae, Francisella tularensis, Anaplasma phagocytophilum, Candidatus Neoehrlichia mikurensis (CNM) and Coxiella burnetii were detected in the most prominent tick in Germany, the hard tick Ixodes ricinus. While infections, such as TBE and Lyme disease are well known, other infections are hardly known even among experts. Although there have been a few descriptions of isolated cases in Germany, a systematic investigation regarding the distribution and the pathogenic potential of these pathogens is still lacking. In particular elderly people and people with underlying diseases seem to be mostly affected. The importance of new infectious disease agents, such as Candidatus Neoehrlichia mikurensis but also of long known pathogens, such as Rickettsiae still remains unclear, while some of them could be detected in 20 % of investigated ticks. Whether climate change contributes to the further distribution of these infectious agents remains unclear and requires further investigation. The increasing initiatives to create natural environments and the trend towards spending more time in nature for recreational activities will increase the danger of coming into contact with ticks and the respective infectious agents. Considering these circumstances an increase of diseases caused by these pathogens is to be expected. © 2014, Springer-Verlag Berlin Heidelberg.


PubMed | Institute For Bakterielle Infektionen Und Zoonosen
Type: Journal Article | Journal: Bundesgesundheitsblatt, Gesundheitsforschung, Gesundheitsschutz | Year: 2016

The relevance for public health of the agent Arcobacter is mostly unclear despite of an increasing number of studies. Recent evidence shows that especially Arcobacter(A.) butzleri but also A.cryaerophilus and A.skirrowii may be involved in human enteric diseases. However, little is currently known about pathogenicity or potential virulence factors. Livestock animals, particularly poultry and pigs, might be asignificant reservoir of Arcobacter spp. Furthermore, Arcobacter spp. could be isolated from retail raw meat products of these animals as well as from drinking water. There are currently no standardized isolation and detection methods to collect comparable data. Further studies and efforts of both human and veterinary medicine are needed to elucidate prevalence, epidemiology, the pathogenic role and potential virulence factors of Arcobacter spp. These data are the necessary basis for further risk assessment.


PubMed | Institute For Bakterielle Infektionen Und Zoonosen
Type: | Journal: BMC veterinary research | Year: 2011

The epidemiological situation of ovine chlamydial infections in continental Europe, especially Germany is poorly characterised. Using the German state of Thuringia as a model example, the chlamydial sero- and antigen prevalence was estimated in thirty-two randomly selected sheep flocks with an average abortion rate lower than 1%. Seven vaccinated flocks were reviewed separately.A wide range of samples from 32 flocks were examined. Assumption of a seroprevalence of 10% (CI 95%) at flock level, revealed that 94% of the tested flocks were serologically positive with ongoing infection (i.e. animals with seroconversion) in nearly half (47%) of the flocks. On the basis of an estimated 25% antigen prevalence (CI 95%), PCR and DNA microarray testing, together with sequencing revealed the presence of chlamydiae in 78% of the flocks. The species most frequently found was Chlamydophila (C.) abortus (50%) followed by C. pecorum (47%) and C. psittaci genotype A (25%). Mixed infections occurred in 25% of the tested flocks. Samples obtained from the vaccinated flocks revealed the presence of C. abortus field samples in 4/7 flocks. C. pecorum was isolated from 2/7 flocks and the presence of seroconversion was determined in 3/7 flocks.The results imply that chlamydial infections occur frequently in German sheep flocks, even in the absence of elevated abortion rates. The fact that C. pecorum and the potentially zoonotic C. psittaci were found alongside the classical abortifacient agent C. abortus, raise questions about the significance of this reservoir for animal and human health and underline the necessity for regular monitoring. Further studies are needed to identify the possible role of C. psittaci infections in sheep.

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